Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,100,669 G→T 5.0% A200A (GCC→GCA gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,100,6690GT 5.0% 271.0 / 4.7 80A200A (GCC→GCAgnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base G (48/28);  new base T (2/2);  total (50/30)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

CCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTAC  >  NC_000913/2100521‑2100815
                                                                                                                                                    |                                                                                                                                                  
ccAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAg                                                                                                                                                    <  1:67995/149‑1 (MQ=255)
 cAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAgg                                                                                                                                                   <  1:115065/149‑1 (MQ=255)
  aGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGc                                                                                                                                                  >  3:84950/1‑149 (MQ=255)
  aGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGc                                                                                                                                                  <  1:257288/149‑1 (MQ=255)
    gTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCtt                                                                                                                                                >  1:856380/1‑149 (MQ=255)
     tagtccccGTCGTCAGCTTGTTTGGTTAAGATATCTTTGGTTATGTCGATCAGGTAACTGCACGGTGCACCGTATTACCACTCGCTAAAGGTCTGTTACAGTTCTGCGTGAATCAGTTTCATGCCACGTATAAGAAGAAAAGAGGGTTc                                                                                                                                               <  2:73674/143‑1 (MQ=255)
       gTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAg                                                                                                                                             >  3:115282/1‑149 (MQ=255)
       gTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAg                                                                                                                                             <  3:188462/149‑1 (MQ=255)
       gTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAg                                                                                                                                             <  1:31422/149‑1 (MQ=255)
           ccGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATAACGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTACGCGCCAGTTCCTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAGTAGGCGTCAGCAAt                                                                                                                                         >  4:542844/1‑149 (MQ=255)
             gTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCa                                                                                                                                       >  4:611383/1‑149 (MQ=255)
                   aTCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCa                                                                                                                                 >  1:204357/1‑149 (MQ=255)
                    tCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCat                                                                                                                                >  2:848888/1‑149 (MQ=255)
                    tCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCat                                                                                                                                >  2:135839/1‑149 (MQ=255)
                    tCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCat                                                                                                                                >  4:29117/1‑149 (MQ=255)
                            ggTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGt                                                                                                                        <  4:115282/149‑1 (MQ=255)
                               gAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTAtt                                                                                                                     <  3:611383/149‑1 (MQ=255)
                                  gATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCaa                                                                                                                  >  3:684093/1‑149 (MQ=255)
                                  gATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCaa                                                                                                                  >  2:368569/1‑149 (MQ=255)
                                    tatCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCATTTCTTCGTTGGTGAGGTACAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATa                                                                                                                >  2:464269/1‑149 (MQ=255)
                                     atCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATATGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATAc                                                                                                               >  2:362400/1‑149 (MQ=255)
                                     atCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATATGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATAc                                                                                                               >  4:43612/1‑149 (MQ=255)
                                     atCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATAc                                                                                                               >  3:800068/1‑149 (MQ=255)
                                         ttGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGtt                                                                                                           <  3:142130/149‑1 (MQ=255)
                                         ttGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGtt                                                                                                           <  1:655533/149‑1 (MQ=255)
                                           ggTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTtgt                                                                                                         <  4:617881/149‑1 (MQ=255)
                                             tGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATATGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGa                                                                                                       <  1:362400/149‑1 (MQ=255)
                                             tGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATATGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGa                                                                                                       <  3:43612/149‑1 (MQ=255)
                                              gATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGaa                                                                                                      <  1:185850/149‑1 (MQ=255)
                                              gATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGaa                                                                                                      >  2:153306/1‑149 (MQ=255)
                                              gATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGaa                                                                                                      <  2:69837/149‑1 (MQ=255)
                                                tGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAAcc                                                                                                    >  2:244378/1‑149 (MQ=255)
                                                 gTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                                                                   >  3:239135/1‑149 (MQ=255)
                                                   cGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATc                                                                                                 <  2:19940/149‑1 (MQ=255)
                                                    gATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCt                                                                                                >  1:158670/1‑149 (MQ=255)
                                                    gATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCt                                                                                                >  1:11993/1‑149 (MQ=255)
                                                          gTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGATTAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACAt                                                                                          >  4:57204/1‑149 (MQ=255)
                                                          gTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACAt                                                                                          >  1:114366/1‑149 (MQ=255)
                                                             aCTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGt                                                                                       <  3:614506/149‑1 (MQ=255)
                                                             aCTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGt                                                                                       <  4:17839/149‑1 (MQ=255)
                                                              cTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCATAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTg                                                                                      <  4:684093/149‑1 (MQ=255)
                                                                       tCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTgcgac                                                                             <  2:11993/149‑3 (MQ=255)
                                                                         aCCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTg                                                                                      >  4:290715/1‑138 (MQ=255)
                                                                         aCCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTg                                                                                      <  3:290715/138‑1 (MQ=255)
                                                                         aCCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCAt                                                                           >  3:194106/1‑149 (MQ=255)
                                                                         aCCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCAt                                                                           >  1:387855/1‑149 (MQ=255)
                                                                         aCCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCAt                                                                           >  2:602174/1‑149 (MQ=255)
                                                                         aCCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGACAATAGGCTTCAGCAATCGGCTGCTTATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCAt                                                                           >  3:616778/1‑149 (MQ=255)
                                                                          ccGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                                                                   <  1:441820/124‑1 (MQ=255)
                                                                          ccGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCa                                                                                                   >  2:441820/1‑124 (MQ=255)
                                                                          ccGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATc                                                                          <  4:460048/149‑1 (MQ=255)
                                                                                ttCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCAcc                                                                    >  3:688153/1‑149 (MQ=255)
                                                                                 tCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCa                                                                   >  3:226931/1‑149 (MQ=255)
                                                                                  ccACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCaa                                                                  <  3:29117/149‑1 (MQ=255)
                                                                                   cACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGt                                                                                       <  2:120065/127‑1 (MQ=255)
                                                                                   cACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGt                                                                                       >  1:120065/1‑127 (MQ=255)
                                                                                    aCTGGGTGAACGTCTGCGAGAGATATTGGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAGTCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAata                                                                >  4:808500/1‑149 (MQ=255)
                                                                                    aCTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAata                                                                >  4:263834/1‑149 (MQ=255)
                                                                                     cTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAatat                                                               >  3:673933/1‑149 (MQ=255)
                                                                                      tCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAAtata                                                              <  2:409176/149‑1 (MQ=255)
                                                                                                 cTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATg                                                   <  3:419415/149‑1 (MQ=255)
                                                                                                      ccAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCa                                              >  4:304283/1‑149 (MQ=255)
                                                                                                      ccAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCa                                              >  3:178469/1‑149 (MQ=255)
                                                                                                       cAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCAc                                             <  2:18382/149‑1 (MQ=255)
                                                                                                          ttcttcGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGt                                          >  2:65162/1‑149 (MQ=255)
                                                                                                            cttcGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCt                                        >  2:810245/1‑149 (MQ=255)
                                                                                                             ttcGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCtt                                       >  2:38996/1‑149 (MQ=255)
                                                                                                             ttcGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCtt                                       >  2:253479/1‑149 (MQ=255)
                                                                                                               cGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCa                                     <  4:239135/149‑1 (MQ=255)
                                                                                                                gTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAg                                    >  4:706172/1‑149 (MQ=255)
                                                                                                                   ggTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTa                                 >  4:335044/1‑149 (MQ=255)
                                                                                                                                  aCCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTgg                  >  3:581255/1‑149 (MQ=255)
                                                                                                                                  aCCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTgg                  >  2:233966/1‑149 (MQ=255)
                                                                                                                                   ccTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGt                 <  3:202455/149‑1 (MQ=255)
                                                                                                                                    cTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTc                >  2:825876/1‑149 (MQ=255)
                                                                                                                                     tttAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTCa               >  1:728246/1‑149 (MQ=255)
                                                                                                                                          gCAGAGAATAGTCTTCAGCAATCAGCTGCATACCGCCGTATTCAATACCGTTGGGAACCATCTTCACTTAGTGACCTGCGCCACCCGCACCCATATAGGTAACGCATGGTTCACCGCCTTCAGCTACGGCGGCGATTTTGGTCAGGATc          >  4:62646/1‑149 (MQ=255)
                                                                                                                                          gCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTCCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTCAGGATc          >  3:398700/1‑149 (MQ=255)
                                                                                                                                          gCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATATTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCGGCTACGGCGGCGATTTTGGTCAGGATc          >  2:779538/1‑149 (MQ=255)
                                                                                                                                            agagAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTCAGGATCgg        >  2:75770/1‑149 (MQ=255)
                                                                                                                                                 aTAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTa   <  4:673933/149‑1 (MQ=255)
                                                                                                                                                  tAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTAc  <  1:844975/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCAGGTAGTTACCGTCTTCATCTTTTTTGGTGAAGATATCTTTGGTGATGTCGATCAGGTAACTGCTCAGTTCACCGTTATTCCACTCGGTAAAGGTCTGCGCCAGTTCTTCGTTGGTGAGGTTCAGGCCACCTTTAAGCAGAGAATAGGCTTCAGCAATCAGCTGCATATCGCCGTATTCAATACCGTTGTGAACCATCTTCACATAGTGACCTGCGCCATCGGCACCAATATAGGTAACGCATGGTTCACCGTCTTCAGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTAC  >  NC_000913/2100521‑2100815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: