| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,282,832 | C→G | 10.7% | intergenic (+31/+108) | rplY → / ← yejK | 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,282,832 | 0 | C | G | 10.7% | 303.9 / 19.0 | 92 | intergenic (+31/+108) | rplY/yejK | 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein |
| Reads supporting (aligned to +/- strand): ref base C (44/38); new base G (7/3); total (51/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
AGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTC > NC_000913/2282688‑2282975 | aGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcg > 1:232728/1‑149 (MQ=255)aGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcg > 3:431068/1‑149 (MQ=255) gCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcgg < 3:224395/149‑1 (MQ=255) gCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg‑gcgg < 1:501319/149‑1 (MQ=255) gAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCgggg > 4:786935/1‑149 (MQ=255) ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGtttt > 1:233581/1‑149 (MQ=255) ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATCGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGtttt < 2:854095/149‑1 (MQ=255) cTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGtttttt < 3:695197/149‑1 (MQ=255) atcATCGTTGTTGACGGTAAAGAAATCAAGGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAAATGCTGAATAAGTTGTAAAAAACTCCGCTCCG‑GCGGGGTCTTTTGTa > 4:34727/3‑149 (MQ=255) aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGtttttt < 4:375043/146‑1 (MQ=255) aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGtttttt > 3:375043/1‑146 (MQ=255) cATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATc > 1:726533/1‑149 (MQ=255) aTCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGttttt > 3:97063/1‑142 (MQ=255) aTCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGttttt < 4:97063/142‑1 (MQ=255) aTCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGttttttacaact > 3:460104/1‑143 (MQ=255) gACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTAt > 1:587209/1‑149 (MQ=255) cGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGc < 1:416834/149‑1 (MQ=255) gTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCt > 4:519430/1‑149 (MQ=255) tAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTg > 1:167555/1‑149 (MQ=255) aaaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGa > 3:223771/1‑149 (MQ=255) aaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGttt > 4:104832/1‑124 (MQ=255) aaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGttt < 3:104832/124‑1 (MQ=255) aGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGAtt > 4:82644/1‑149 (MQ=255) aaaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAATCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTAc < 2:761079/149‑1 (MQ=255) aTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACgg > 4:205525/1‑149 (MQ=255) tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGcc > 2:704789/1‑139 (MQ=255) tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGcc < 1:704789/139‑1 (MQ=255) tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGt > 4:878992/1‑149 (MQ=255) tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGttttt > 4:462379/1‑120 (MQ=255) tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGC‑CGGAGCGGGGttttt < 3:462379/120‑1 (MQ=255) cAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa < 3:559209/149‑1 (MQ=255) aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAAGCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCAGATTACGGTAt < 3:581587/149‑1 (MQ=255) aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGtttttt < 1:485291/119‑1 (MQ=255) aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGtttttt > 2:485291/1‑119 (MQ=255) aaaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAt > 3:300223/1‑149 (MQ=255) ttAAAGCTCAGGACGTACGGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTGGTTCGCGCTTAATTGCTGAATAAGTTGTAACAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTgat > 4:112421/1‑147 (MQ=255) ttAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGttttt < 2:526179/114‑1 (MQ=255) ttAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGttttt > 1:526179/1‑114 (MQ=255) ttAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCt < 2:726533/149‑1 (MQ=255) ttAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCt > 2:810659/1‑149 (MQ=255) aaaGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAt > 3:172688/1‑149 (MQ=255) gCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTAATTACGGTATTGCTAtttt < 2:233581/149‑1 (MQ=255) tCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt > 2:625327/1‑149 (MQ=255) tCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt > 4:734797/1‑149 (MQ=255) ggACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCa > 3:751295/1‑149 (MQ=255) gACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAt > 4:825112/1‑134 (MQ=255) gACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAt < 3:825112/134‑1 (MQ=255) gACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg > 2:671990/1‑149 (MQ=255) gACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATAGCTATTTTTTGCAg < 1:671990/149‑1 (MQ=255) gTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGcc > 4:824222/1‑149 (MQ=255) aGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGGTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGAt < 4:300223/149‑1 (MQ=255) cGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTTCAGGCCAGATaa < 4:223771/149‑1 (MQ=255) cGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATaa < 4:751295/149‑1 (MQ=255) aCCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCg > 4:5171/1‑149 (MQ=255) aCCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGA‑GCGGGGtttt < 1:537153/89‑1 (MQ=38) ccGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGtt > 3:590771/1‑149 (MQ=255) gTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATGGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCa < 4:580507/149‑1 (MQ=255) tACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAc > 3:537597/1‑149 (MQ=255) aaaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc > 4:448951/1‑149 (MQ=255) aCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgccgc > 3:578519/1‑149 (MQ=255) tGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCa > 1:357178/1‑149 (MQ=255) gcagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCATGCCAGATGCGGCGTGAACGCCTTATCTGGcct < 2:232728/149‑3 (MQ=255) cagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAtt > 4:473155/1‑149 (MQ=255) caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa < 2:357178/149‑1 (MQ=255) cTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCAt < 1:702530/130‑1 (MQ=255) cTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCAt > 2:702530/1‑130 (MQ=255) cTTCGTTCGCGCTTAAGTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaa < 3:519430/149‑1 (MQ=255) ttcgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa > 2:24794/1‑142 (MQ=255) ttcgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa < 1:24794/142‑1 (MQ=255) ttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa > 1:475329/1‑149 (MQ=255) gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCATAACACAAAACCGATTa < 2:552203/149‑1 (MQ=255) gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa < 4:128184/146‑1 (MQ=255) gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa > 3:128184/1‑146 (MQ=255) gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTa < 4:737930/149‑1 (MQ=255) cTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAAtt < 1:490396/149‑1 (MQ=255) tAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc < 3:824222/149‑1 (MQ=255) tAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc < 3:734797/149‑1 (MQ=255) gCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCa < 3:473155/149‑1 (MQ=255) tGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGATCTTACAACCGATTAATTGCCGCCAGa < 4:385862/149‑1 (MQ=255) tGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGa > 3:524545/1‑149 (MQ=255) aaTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCa < 4:647958/82‑1 (MQ=255) aaTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCa > 3:647958/1‑82 (MQ=255) aaTAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACg > 3:392062/1‑149 (MQ=255) gTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgcgc > 4:826040/1‑149 (MQ=255) tAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCt < 4:590771/149‑1 (MQ=255) aaaaaaCCCCGCCGGA‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg > 4:314683/1‑149 (MQ=255) aaaaaaCCCCGCCGGA‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg > 4:163032/1‑149 (MQ=255) aaaaaCCCCGCCGGA‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGc > 2:837999/1‑149 (MQ=255) aaaCCCCGCCGGA‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCaa > 3:655219/1‑149 (MQ=255) aaCCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAAt < 3:205525/149‑1 (MQ=255) cccGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTgg > 1:207799/1‑149 (MQ=255) cccGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTgg > 1:140696/1‑149 (MQ=255) cGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTc < 1:878729/149‑1 (MQ=255) cGCTACG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTc < 3:198605/149‑1 (MQ=255) gCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa > 3:788734/1‑112 (MQ=255) gCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa < 4:788734/112‑1 (MQ=255) | AGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCG‑GCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTC > NC_000913/2282688‑2282975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |