Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,375,484 A→T 11.6% L160H (CTT→CAT) ‡ menC ← O‑succinylbenzoyl‑CoA synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,375,4840AT11.6% 314.8 / 29.2 103L160H (CTT→CAT) ‡menCO‑succinylbenzoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base A (47/44);  new base T (7/5);  total (54/49)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-01

Only 100 of 108 total aligned reads displayed.

GGCAATGCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAT  >  NC_000913/2375336‑2375631
                                                                                                                                                    |                                                                                                                                                   
ggcaatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCaa                                                                                                                                                     >  2:184772/1‑149 (MQ=255)
  caatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGa                                                                                                                                                   <  3:407089/149‑1 (MQ=255)
  caaggCCGGTGTCACGGGCAAACGCTCGCGAAGCATCGCGGGTCTTGCACGGCTCGTCGAGAAACGCGATGCGGGCGCGATAAGCCGGGTTAACGTAGTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGGCAAGa                                                                                                                                                   <  2:634837/149‑1 (MQ=255)
   aatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGAc                                                                                                                                                  >  3:173854/1‑149 (MQ=255)
   aatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGAc                                                                                                                                                  >  3:20559/1‑149 (MQ=255)
   aatgCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGAc                                                                                                                                                  <  2:218198/149‑1 (MQ=255)
         ggTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCTTGACGCAAAt                                                                                                                                            <  1:221039/149‑1 (MQ=255)
         ggTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAAt                                                                                                                                            >  4:150161/1‑149 (MQ=255)
          gTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCTTGACGCAAAtc                                                                                                                                           <  4:154518/149‑2 (MQ=255)
              cACGGGCAAACGCTAGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGACACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGAAGa                                                                                                                                       <  2:750327/149‑1 (MQ=255)
                cGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATc                                                                                                                                     <  3:410388/149‑1 (MQ=255)
                  ggCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCg                                                                                                                                   <  2:346385/149‑1 (MQ=255)
                    cAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGa                                                                                                                                 <  2:638867/149‑1 (MQ=255)
                    cAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGat                                                                                                                                <  4:572692/149‑2 (MQ=255)
                     aaaCGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGaa                                                                                                                                >  4:27676/1‑149 (MQ=255)
                         gCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGc                                                                                                                            >  2:107372/1‑149 (MQ=255)
                         gCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGc                                                                                                                            <  4:206086/149‑1 (MQ=255)
                           tCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAAt                                                                                                                                            >  2:122487/1‑131 (MQ=255)
                           tCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAAt                                                                                                                                            <  1:122487/131‑1 (MQ=255)
                           tCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCt                                                                                                                          >  1:786467/1‑149 (MQ=255)
                           tCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCt                                                                                                                          >  3:822475/1‑149 (MQ=255)
                             gcgAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTcc                                                                                                                        >  4:613927/1‑149 (MQ=255)
                                  tcatcGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCaa                                                                                                                   >  2:365658/1‑149 (MQ=255)
                                      cgcgGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGa                                                                                                               <  1:816851/149‑1 (MQ=255)
                                      cgcgGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGa                                                                                                               <  3:354473/149‑1 (MQ=255)
                                         gggTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTc                                                                                                            >  1:727748/1‑149 (MQ=255)
                                          ggTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTca                                                                                                           <  4:277354/149‑1 (MQ=255)
                                           gTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGATTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTcac                                                                                                          >  3:249731/1‑149 (MQ=255)
                                                   cGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGc                                                                                                  <  2:754878/149‑1 (MQ=255)
                                                    ggCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGcc                                                                                                 >  1:813542/1‑149 (MQ=255)
                                                    ggCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGcc                                                                                                 >  3:444569/1‑149 (MQ=255)
                                                         ttagagaAACGCGATGCGGTCGCGATAATCAGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  2:292270/4‑149 (MQ=255)
                                                         ttCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGTCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  4:719276/1‑149 (MQ=255)
                                                         ttCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  2:682045/1‑149 (MQ=255)
                                                         ttCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  4:822456/1‑149 (MQ=255)
                                                         ttCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  3:510616/1‑149 (MQ=255)
                                                         ttCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  1:680839/1‑149 (MQ=255)
                                                            gagaAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCa                                                                                         <  1:256779/149‑1 (MQ=255)
                                                               aaaCGCTATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCAcct                                                                                      >  4:584815/1‑148 (MQ=255)
                                                                      aTGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTa                                                                               <  4:444569/149‑1 (MQ=255)
                                                                             cgcgATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTc                                                                                                            >  1:801371/1‑113 (MQ=255)
                                                                             cgcgATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTc                                                                                                            <  2:801371/113‑1 (MQ=255)
                                                                              gcgATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCa                                                                       <  1:898141/149‑1 (MQ=255)
                                                                              gcgATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCa                                                                       <  2:727748/149‑1 (MQ=255)
                                                                                gATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACt                                                                     >  4:811187/1‑149 (MQ=255)
                                                                                gATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACt                                                                     >  2:546787/1‑149 (MQ=255)
                                                                                  tAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACttt                                                                   <  2:139463/149‑1 (MQ=255)
                                                                                   aaTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTg                                                                  >  4:419142/1‑149 (MQ=255)
                                                                                    aTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGc                                                                                                  >  2:742964/1‑116 (MQ=255)
                                                                                    aTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGc                                                                                                  <  1:742964/116‑1 (MQ=255)
                                                                                    aTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGa                                                                 >  4:235160/1‑149 (MQ=255)
                                                                                    aTCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGa                                                                 <  2:813002/149‑1 (MQ=255)
                                                                                      ccGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGCTTCACCACCCGGCCGACCCGCACCGCTTCCCACCATCCCACTCTgcac                                                               <  2:618382/149‑4 (MQ=255)
                                                                                          gTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCg                                                           >  3:809861/1‑149 (MQ=255)
                                                                                            tAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGcc                                                         >  1:298081/1‑149 (MQ=255)
                                                                                            tAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGcc                                                         <  2:786467/149‑1 (MQ=255)
                                                                                              aCGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCAc                                                       >  4:120972/1‑149 (MQ=255)
                                                                                              aCGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCAACACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCAc                                                       <  2:680839/149‑1 (MQ=255)
                                                                                                 tATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACttt                                                    <  1:107372/149‑1 (MQ=255)
                                                                                                    ttGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTtctc                                                 <  1:365658/149‑1 (MQ=255)
                                                                                                        cAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCTAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCt                                             <  2:551218/149‑1 (MQ=255)
                                                                                                           aCTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGc                                          >  1:830861/1‑149 (MQ=255)
                                                                                                            ctgctgACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCa                                         >  3:130504/1‑149 (MQ=255)
                                                                                                                 gACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCt                                    >  1:219333/1‑149 (MQ=255)
                                                                                                                  aCCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTg                                   >  3:871318/1‑149 (MQ=255)
                                                                                                                    cTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCa                                 >  4:155986/1‑149 (MQ=255)
                                                                                                                     tttCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTcgc                                                                                            >  4:32311/1‑89 (MQ=255)
                                                                                                                     tttCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCaa                                >  2:601632/1‑149 (MQ=255)
                                                                                                                     tttCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCaa                                <  2:194836/149‑1 (MQ=255)
                                                                                                                     tttCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCaa                                <  2:270519/149‑1 (MQ=255)
                                                                                                                           cGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGa                          <  1:682045/149‑1 (MQ=255)
                                                                                                                            ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         <  3:403465/149‑1 (MQ=255)
                                                                                                                            ggTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         <  4:280209/149‑1 (MQ=255)
                                                                                                                               gtCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGAt                      <  2:811024/149‑1 (MQ=255)
                                                                                                                               gtCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGAt                      >  2:382747/1‑149 (MQ=255)
                                                                                                                                  cAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCaa                                >  1:821795/1‑136 (MQ=255)
                                                                                                                                  cAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCaa                                <  2:821795/136‑1 (MQ=255)
                                                                                                                                   aGGCGCGACTTGCGGCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGGACAATACCACTTTGACCTGCGCTACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGa                  <  3:738237/149‑1 (MQ=255)
                                                                                                                                    ggCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGAt                 >  4:817434/1‑149 (MQ=255)
                                                                                                                                        cgATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTc             >  1:11585/1‑149 (MQ=255)
                                                                                                                                         gATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCt                                    >  1:602642/1‑125 (MQ=255)
                                                                                                                                         gATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCt                                    <  2:602642/125‑1 (MQ=255)
                                                                                                                                         gATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTcc            <  4:871318/149‑1 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAAc                                                                                                                  <  3:794046/46‑1 (MQ=25)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAAc                                                                                                                  >  4:794046/1‑46 (MQ=37)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCAcc                                                                                       >  4:30195/1‑73 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCAcc                                                                                       <  3:30195/73‑1 (MQ=39)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           >  2:447049/1‑149 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           >  1:10055/1‑149 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           >  1:183183/1‑149 (MQ=255)
                                                                                                                                          aTTTGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCg           >  1:230310/1‑149 (MQ=255)
                                                                                                                                           tttGCGTCTTGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCgg          >  2:574588/1‑149 (MQ=255)
                                                                                                                                           tttGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCgg          >  3:804552/1‑149 (MQ=255)
                                                                                                                                              gCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATc       <  1:750204/149‑1 (MQ=255)
                                                                                                                                                gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGTCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGc     >  1:15189/1‑149 (MQ=255)
                                                                                                                                                gTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGc     <  1:184772/149‑1 (MQ=255)
                                                                                                                                                   aaGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAt  <  3:811187/149‑1 (MQ=255)
                                                                                                                                                   aaGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAt  <  4:880081/149‑1 (MQ=255)
                                                                                                                                                    aGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         <  3:264663/125‑1 (MQ=255)
                                                                                                                                                    aGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAg                         >  4:264663/1‑125 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCAATGCCGGTTTCACGGGCAAACGCTCGCGAATCATCGCGGGTTTTGCACGGCTCTTCGAGAAACGCGATGCGGTCGCGATAATCCGGGTTAACGTATTTGGCAAACTGCTGACCTTTCAGCGGTGTCCAGGCGCGATTTGCGTCAAGACGCAAATGCAGATCCGGAATTGCCTCCAGCAACAGATTCACCACCATGCCGTCGCGCACCGCTTCGTACAATCCCACTTTGACCTTCGCCACTTTCTCGCCTGGCATATCTGCAAGTTTGAGGATCAGATCGTCCGGATCGCCAT  >  NC_000913/2375336‑2375631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: