Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,536,110 G→A 100% G93S (GGT→AGT)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,1100GA100.0% 473.2 / ‑6.7 129G93S (GGT→AGT) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (58/70);  total (58/71)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 129 total aligned reads displayed.

AAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATC  >  NC_000913/2535965‑2536256
                                                                                                                                                 |                                                                                                                                                  
aaaaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTa                                                                                                                                                 >  1:695555/1‑149 (MQ=255)
 aaaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTAt                                                                                                                                                >  2:896656/1‑149 (MQ=255)
  aaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATc                                                                                                                                               <  2:170485/149‑1 (MQ=255)
  aaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATc                                                                                                                                               >  4:393736/1‑149 (MQ=255)
  aaaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGAAAGCGGCGTTGAACTGTTCGTCCACTTCAGTATc                                                                                                                                               <  1:446035/149‑1 (MQ=255)
   aaTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCg                                                                                                                                              >  3:16866/1‑149 (MQ=255)
    aTCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGa                                                                                                                                             >  3:693542/1‑149 (MQ=255)
     tCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGac                                                                                                                                            <  1:61170/149‑1 (MQ=255)
           gTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGtt                                                                                                                                      <  2:79985/149‑1 (MQ=255)
            tGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTg                                                                                                                                     <  4:305903/149‑1 (MQ=255)
              aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGaa                                                                                                                                   <  1:721302/149‑1 (MQ=255)
              aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGaa                                                                                                                                   >  2:125452/1‑149 (MQ=255)
                    ttGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGaaa                                                                                                                             <  1:403038/149‑1 (MQ=255)
                    ttGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGaaa                                                                                                                             <  1:133920/149‑1 (MQ=255)
                      gCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAgg                                                                                                                           <  3:112607/149‑1 (MQ=255)
                       cTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGc                                                                                                                          >  3:741597/1‑149 (MQ=255)
                        tATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCg                                                                                                                         >  4:821565/1‑149 (MQ=255)
                         aTCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGa                                                                                                                        >  2:537446/1‑149 (MQ=255)
                         aTCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGa                                                                                                                        >  3:193464/1‑149 (MQ=255)
                          tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGaa                                                                                                                       >  1:721864/1‑149 (MQ=255)
                          tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGaa                                                                                                                       >  3:84534/1‑149 (MQ=255)
                          tCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGaa                                                                                                                       <  3:524596/149‑1 (MQ=255)
                           cAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAg                                                                                                                      >  4:355382/1‑149 (MQ=255)
                             aaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGc                                                                                                                    >  1:212108/1‑149 (MQ=255)
                                cAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTc                                                                                                                 >  3:302815/1‑149 (MQ=255)
                                    gggTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGc                                                                                                             <  1:407899/149‑1 (MQ=255)
                                    gggTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGc                                                                                                             <  4:16866/149‑1 (MQ=255)
                                         aCAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTAtt                                                                                                        >  4:655506/1‑149 (MQ=255)
                                            aaaTGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCt                                                                                                     <  1:537446/149‑1 (MQ=255)
                                            aaaTGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCt                                                                                                     <  2:450194/149‑1 (MQ=255)
                                            aaaTGGTCGCGCCAGTAGACAGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCt                                                                                                     >  2:110308/1‑149 (MQ=255)
                                             aaTGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTg                                                                                                    <  4:724084/149‑1 (MQ=255)
                                                 gTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaaga                                                                                                <  2:572384/149‑1 (MQ=255)
                                                   cgcgCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaagaat                                                                                              <  4:515022/149‑2 (MQ=255)
                                                   cgcgCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGaagaag                                                                                              <  2:721864/149‑1 (MQ=255)
                                                     cgcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGt                                                                                            <  4:187602/149‑1 (MQ=255)
                                                     cgcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGt                                                                                            <  3:593001/149‑1 (MQ=255)
                                                      gcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTc                                                                                           <  1:637302/149‑1 (MQ=255)
                                                       ccAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCa                                                                                          <  1:658065/149‑1 (MQ=255)
                                                          gTAGACGGCACCATTGGTAAAATCTTTGAAAGCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAgcg                                                                                       <  4:764268/149‑1 (MQ=255)
                                                          gTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGTTGAAGAAGGTCAgcg                                                                                       >  3:259071/1‑149 (MQ=255)
                                                          gTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAgcg                                                                                       >  3:115319/1‑149 (MQ=255)
                                                                   aCCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGt                                                                              >  1:830735/1‑149 (MQ=255)
                                                                    ccATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGtt                                                                                                                                      >  3:661710/1‑92 (MQ=255)
                                                                     cATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGt                                                                                    >  3:675363/1‑141 (MQ=255)
                                                                     cATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGt                                                                                    <  4:675363/141‑1 (MQ=255)
                                                                     cATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTg                                                                            >  2:498704/1‑149 (MQ=255)
                                                                       ttGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc                                                                          <  4:693542/149‑1 (MQ=255)
                                                                       ttGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc                                                                          >  1:874159/1‑149 (MQ=255)
                                                                       ttGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc                                                                          <  1:125452/149‑1 (MQ=255)
                                                                          gTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGAt                                                                       >  3:565037/1‑149 (MQ=255)
                                                                          gTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGAt                                                                       >  4:830283/1‑149 (MQ=255)
                                                                           tAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                                      <  2:695555/149‑1 (MQ=255)
                                                                           tAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                                      >  2:55887/1‑149 (MQ=255)
                                                                           tAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                                      >  2:269523/1‑149 (MQ=255)
                                                                             aaaTCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACt                                                                    >  2:689684/1‑149 (MQ=255)
                                                                                tCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc                                                                          <  2:618977/140‑1 (MQ=255)
                                                                                tCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTc                                                                 <  1:624532/149‑1 (MQ=255)
                                                                                       aaccaaccACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAt                                                          >  4:259967/1‑149 (MQ=255)
                                                                                         ccaaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAttt                                                        <  3:139520/149‑1 (MQ=255)
                                                                                         ccaaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAttt                                                        <  1:326936/149‑1 (MQ=255)
                                                                                         ccaaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAttt                                                        <  3:655506/149‑1 (MQ=255)
                                                                                         ccaaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAttt                                                        >  4:372912/1‑149 (MQ=255)
                                                                                           aaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGa                                                      <  3:747017/149‑1 (MQ=255)
                                                                                           aaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGa                                                      <  4:84534/149‑1 (MQ=255)
                                                                                           aaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGa                                                      <  1:2388/149‑1 (MQ=255)
                                                                                           aaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGa                                                      >  2:508033/1‑149 (MQ=255)
                                                                                            accaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGTGTGAAAGTTGGCGATACTGTCATTGAATTTGAt                                                     >  1:206037/1‑149 (MQ=255)
                                                                                             ccaCACATTCTCTACCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGTGTGAAAGTTGGCGATACTGTCATTGAATTTGATc                                                    <  2:206037/149‑1 (MQ=255)
                                                                                               aCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTg                                                  >  1:187281/1‑149 (MQ=255)
                                                                                               aCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTg                                                  >  3:356362/1‑149 (MQ=255)
                                                                                                      ctctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCgctgc                                           <  2:212153/149‑1 (MQ=255)
                                                                                                      ctctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCgctgc                                           <  1:606076/149‑1 (MQ=255)
                                                                                                       tctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCgctgct                                          >  4:450324/1‑149 (MQ=255)
                                                                                                       tctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCgctgct                                          >  2:327810/1‑149 (MQ=255)
                                                                                                        ctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGctgctg                                         <  1:466193/149‑1 (MQ=255)
                                                                                                         tATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTgg                                        >  1:826770/1‑149 (MQ=255)
                                                                                                         tATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTgg                                        <  4:115319/149‑1 (MQ=255)
                                                                                                           tCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGaa                                      >  4:673318/1‑149 (MQ=255)
                                                                                                           tCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGaa                                      <  1:315786/149‑1 (MQ=255)
                                                                                                           tCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGaa                                      <  3:373057/149‑1 (MQ=255)
                                                                                                             gAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAaga                                    <  3:839316/149‑1 (MQ=255)
                                                                                                                 cTGATAGCGGCGTTGAACTGTTCGTCCACTTCATTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCGGAAGAAGGTCAGCGCGTGAAAGTTGTCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGaaa                                <  2:760005/149‑1 (MQ=255)
                                                                                                                 cTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGaaa                                <  3:474331/149‑1 (MQ=255)
                                                                                                                   gATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                              >  2:134572/1‑149 (MQ=255)
                                                                                                                      cgcggcgTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTCTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCaa                           <  1:57355/148‑1 (MQ=255)
                                                                                                                      aGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCaa                           <  1:30102/149‑1 (MQ=255)
                                                                                                                                aaCTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTg                 >  4:665317/1‑149 (MQ=255)
                                                                                                                                  cTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTAc                     >  1:63794/1‑143 (MQ=255)
                                                                                                                                  cTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTAc                     <  2:63794/143‑1 (MQ=255)
                                                                                                                                   tGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACt              >  3:394259/1‑149 (MQ=255)
                                                                                                                                        gTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGa                                                      <  4:144202/104‑1 (MQ=255)
                                                                                                                                         tCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgtt        >  2:40360/1‑149 (MQ=255)
                                                                                                                                            aCTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAt                                                          >  3:507418/1‑96 (MQ=255)
                                                                                                                                            aCTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt     <  2:788309/149‑1 (MQ=255)
                                                                                                                                            aCTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt     >  4:797144/1‑149 (MQ=255)
                                                                                                                                            aCTTCAGTATCGACACCGTTGAACTGAAAGACGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAAt                                                          <  4:507418/96‑1 (MQ=255)
                                                                                                                                              ttCGGTACCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCGGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAG‑AAGCCAAGTCTACCCTGACTCCGGTTGTTAtc  <  1:116782/149‑1 (MQ=255)
                                                                                                                                              ttCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAt   >  4:304623/1‑149 (MQ=255)
                                                                                                                                              ttCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCAGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAt   >  2:640350/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATC  >  NC_000913/2535965‑2536256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑