Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,536,370 C→G 12.3% intergenic (+27/+16) crr → / ← pdxK glucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,3700CG12.3% 337.9 / 34.4 106intergenic (+27/+16)crr/pdxKglucose‑specific enzyme IIA component of PTS/pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base C (44/49);  new base G (7/6);  total (51/55)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

Only 100 of 107 total aligned reads displayed.

GAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCAC  >  NC_000913/2536223‑2536516
                                                                                                                                                   |                                                                                                                                                  
gaAAGCCAAGTCTACCCAGACTCCGGTTGTTATCTCCAACATGGACGAAGTCAAAGAACTGATCAAACTTTCCGGTAGCGTAACCGTGGGTGTAACCCCGGTTATCCGCATCAATAAGCAATTCTTGCCGCAGTGAAGAATGGCGCCCa                                                                                                                                                   >  4:545106/1‑149 (MQ=255)
   aGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTTAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGAtgg                                                                                                                                                >  4:315047/1‑147 (MQ=255)
   aGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCg                                                                                                                                                >  2:676476/1‑149 (MQ=255)
    gCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCgg                                                                                                                                               >  3:436606/1‑149 (MQ=255)
     ccAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGgc                                                                                                                                              <  3:117373/149‑1 (MQ=255)
      cAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGgcg                                                                                                                                             >  2:274854/1‑149 (MQ=255)
      cAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGgcg                                                                                                                                             <  1:615544/149‑1 (MQ=255)
           cTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAtt                                                                                                                                        <  3:190643/149‑1 (MQ=255)
            tACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAttt                                                                                                                                       <  3:653898/149‑1 (MQ=255)
            tACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAttt                                                                                                                                       <  3:191781/149‑1 (MQ=255)
            tACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAttt                                                                                                                                       <  3:315047/149‑1 (MQ=255)
            tACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttt                                                                                                                                       <  3:497930/149‑1 (MQ=255)
              cccTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAttttt                                                                                                                                     <  2:826770/149‑1 (MQ=255)
              cccTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttttt                                                                                                                                     >  3:817816/1‑149 (MQ=255)
               ccTGACTCCGGTTGTTATCTCCAACATTGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAtttttt                                                                                                                                    <  3:610910/149‑1 (MQ=255)
               ccTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAtttttt                                                                                                                                    >  2:15585/1‑149 (MQ=255)
                cTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttttttt                                                                                                                                   <  3:501781/149‑1 (MQ=255)
                cTGACTCCAGTTGTGATCTCCAACATGGACGGAATCAAAGAGCTGATCAAACTGTCCGGTAGCGTAACCGGGTGTGAAACCCCGGTGATCCGGATCAAGAAGTAAATCTGGCCGCAGTGAAAAATGGCGCCCATCGGCGCCAttttttt                                                                                                                                   <  3:545106/149‑1 (MQ=255)
                 tGACTCGGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                                                                                  <  1:837378/149‑1 (MQ=255)
                 tGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                                                                                  <  1:578277/149‑1 (MQ=255)
                 tGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                                                                                  <  3:402945/149‑1 (MQ=255)
                       cGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTc                                                                                                                            >  3:793453/1‑149 (MQ=255)
                       cGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTc                                                                                                                            <  3:281267/149‑1 (MQ=255)
                         gttgttATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCg                                                                                                                          >  4:564864/1‑149 (MQ=255)
                          ttgttATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGc                                                                                                                         >  2:16696/1‑149 (MQ=255)
                             ttATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                                                                                  >  2:501785/1‑137 (MQ=255)
                             ttATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                                                                                  <  1:501785/137‑1 (MQ=255)
                                tctcCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcgg                                                                                                                   >  3:863239/1‑149 (MQ=255)
                                 ctcCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcggc                                                                                                                  <  2:364350/149‑1 (MQ=255)
                                 ctcCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcggc                                                                                                                  >  1:321902/1‑149 (MQ=255)
                                  tcCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAgcggcg                                                                                                                 >  3:6506/1‑149 (MQ=255)
                                     aaCATGGACGAAATCAAAGAACTGATCAAACTGACCGGTAGCCTAACCGTGGGAGAAACCCCGGTTATCCGTATCAACAACTAATTCCTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTACCCCAGCGGCGGCa                                                                                                              <  4:536246/149‑1 (MQ=255)
                                        aTGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCaaaa                                                                                                           <  4:793453/149‑1 (MQ=255)
                                         tGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAAt                                                                                                          <  1:141188/149‑1 (MQ=255)
                                            aCGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCaa                                                                                                       <  1:487375/149‑1 (MQ=255)
                                             cGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAAt                                                                                                      <  1:16696/149‑1 (MQ=255)
                                              gAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAAtt                                                                                                     >  4:863136/1‑149 (MQ=255)
                                              gAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAAtt                                                                                                     <  4:656613/149‑1 (MQ=255)
                                                 aTCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCAt                                                                                                  >  4:54333/1‑149 (MQ=255)
                                                 aTCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCAt                                                                                                  <  3:446197/149‑1 (MQ=255)
                                                  tCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATc                                                                                                 <  3:131342/149‑1 (MQ=255)
                                                   cAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGATTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                                                                                  <  2:442537/115‑1 (MQ=255)
                                                   cAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGATTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTa                                                                                                                                  >  1:442537/1‑115 (MQ=255)
                                                    aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc                                                                                               >  1:808489/1‑149 (MQ=255)
                                                    aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc                                                                                               >  2:308714/1‑149 (MQ=255)
                                                    aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc                                                                                               >  2:562748/1‑149 (MQ=255)
                                                    aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc                                                                                               >  3:345273/1‑149 (MQ=255)
                                                    aaaGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGc                                                                                               >  3:243809/1‑149 (MQ=255)
                                                      aGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGctc                                                                                             <  4:789743/149‑1 (MQ=255)
                                                           tGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAttt                                                                                                                                       <  3:585238/102‑1 (MQ=255)
                                                           tGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCGATGGGCGCCAttt                                                                                                                                       >  4:585238/1‑102 (MQ=255)
                                                             aTCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtgct                                                                                      >  4:188666/1‑149 (MQ=255)
                                                              tCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCAtgctg                                                                                     >  2:109724/1‑149 (MQ=255)
                                                                  aCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg                                                                                 <  4:653842/149‑1 (MQ=255)
                                                                  aCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg                                                                                 <  3:821565/149‑1 (MQ=255)
                                                                  aCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg                                                                                 <  3:259967/149‑1 (MQ=255)
                                                                  aCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg                                                                                 <  2:106912/149‑1 (MQ=255)
                                                                  aCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTgg                                                                                 <  1:873267/149‑1 (MQ=255)
                                                                      tCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGgtgt                                                                             <  3:56454/149‑1 (MQ=255)
                                                                      tCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAAGTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGgtgt                                                                             >  2:719385/1‑149 (MQ=255)
                                                                       ccGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTa                                                                            <  3:127362/149‑1 (MQ=255)
                                                                         ggTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAgc                                                                          <  3:188666/149‑1 (MQ=255)
                                                                             gCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCAt                                                                      <  2:49750/149‑1 (MQ=255)
                                                                                   ccGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTc                                                                <  1:134572/149‑1 (MQ=255)
                                                                                   ccGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTc                                                                >  2:118445/1‑149 (MQ=255)
                                                                                    cGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTcc                                                               >  1:111197/1‑149 (MQ=255)
                                                                                     gTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCa                                                              >  2:881237/1‑149 (MQ=255)
                                                                                       gggTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCa                                                              <  3:376805/147‑1 (MQ=255)
                                                                                       gggTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCa                                                              >  4:376805/1‑147 (MQ=255)
                                                                                        ggTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTa                                                           >  3:593813/1‑149 (MQ=255)
                                                                                         gTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTAc                                                          >  2:292557/1‑149 (MQ=255)
                                                                                         gTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTAc                                                          >  3:847994/1‑149 (MQ=255)
                                                                                            aaaCCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTAcgcg                                                       >  4:762456/1‑149 (MQ=255)
                                                                                                        tCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCg                                           <  4:847994/149‑1 (MQ=255)
                                                                                                          cGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGt                                         <  4:243809/149‑1 (MQ=255)
                                                                                                           gCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTg                                        >  4:395629/1‑149 (MQ=255)
                                                                                                               cAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACt                                    <  3:175520/149‑1 (MQ=255)
                                                                                                                 agaagTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGc                                  >  1:240611/1‑149 (MQ=255)
                                                                                                                 agaagTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGc                                  >  3:20511/1‑149 (MQ=255)
                                                                                                                  gaagTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCa                                 >  1:257471/1‑149 (MQ=255)
                                                                                                                     gTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCg                              >  3:759082/1‑149 (MQ=255)
                                                                                                                     gTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCg                              >  4:41353/1‑149 (MQ=255)
                                                                                                                       aaTTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGt                            <  2:240611/149‑1 (MQ=255)
                                                                                                                        aTTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGtt                           <  3:41353/149‑1 (MQ=255)
                                                                                                                          tCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTaa                         <  2:257471/149‑1 (MQ=255)
                                                                                                                           cTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAAc                        <  2:792704/149‑1 (MQ=255)
                                                                                                                           cTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCACCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAAc                        <  2:759310/149‑1 (MQ=255)
                                                                                                                            ttGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACg                       <  4:6506/149‑1 (MQ=255)
                                                                                                                                        aaaaTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCa           <  3:372912/149‑1 (MQ=255)
                                                                                                                                         aaaTGGCGCCGATGGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAg          >  2:749633/1‑149 (MQ=255)
                                                                                                                                         aaaTGGCGCCGATGGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGAAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAg          >  3:603228/1‑149 (MQ=255)
                                                                                                                                           aTGGCGCCGATGGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCATTTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCa        >  4:744111/1‑149 (MQ=255)
                                                                                                                                           aTGGCGCCGATGGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCa        >  3:465398/1‑149 (MQ=255)
                                                                                                                                           aTGGCGCCGATGGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCa        >  2:171182/1‑149 (MQ=255)
                                                                                                                                           aTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTc                  <  1:582095/139‑1 (MQ=255)
                                                                                                                                           aTGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTc                  >  2:582095/1‑139 (MQ=255)
                                                                                                                                            tGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCaa       <  1:308714/149‑1 (MQ=255)
                                                                                                                                              gcgcCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAGTTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGc     <  2:737146/149‑1 (MQ=255)
                                                                                                                                                gcCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCa   >  1:346996/1‑149 (MQ=255)
                                                                                                                                                 cccATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCAc  <  4:213141/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCATTTTTTTATGCTTCCGCCAGCGGCGGCAAAATCAATTCATCGCTCTCATGCTGCTGGGTGTAGCGCATCACTTCCAGTACGCGCAACCCCGCTCGGTGCACTGCATCGGTTAACGCCTTCCCTTTCAGCAAGCCAC  >  NC_000913/2536223‑2536516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: