Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 285,887 C→A 5.6% L165M (CTG→ATG)  yagG → CP4‑6 prophage; putative sugar transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913285,8870CA 5.6% 310.9 / 6.9 90L165M (CTG→ATG) yagGCP4‑6 prophage; putative sugar transporter
Reads supporting (aligned to +/- strand):  ref base C (40/45);  new base A (3/2);  total (43/47)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

CTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGG  >  NC_000913/285740‑286031
                                                                                                                                                   |                                                                                                                                                
ctcCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCt                                                                                                                                                 <  4:674319/149‑1 (MQ=255)
 tcCTGACCATGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGACGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCGGCGGCATCGCGCTg                                                                                                                                                >  4:257079/1‑149 (MQ=255)
  ccTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGc                                                                                                                                               <  2:823441/149‑1 (MQ=255)
  ccTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGc                                                                                                                                               >  2:723694/1‑149 (MQ=255)
   cTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGTGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGcc                                                                                                                                              <  4:9147/149‑1 (MQ=255)
       cccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTg                                                                                                                                          >  1:62340/1‑149 (MQ=255)
       cccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTg                                                                                                                                          <  2:311731/149‑1 (MQ=255)
       cccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCCCTATCAGCGGCATCGCGCTGCCGCTg                                                                                                                                          <  4:754201/149‑1 (MQ=255)
        ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTgg                                                                                                                                         >  1:894951/1‑149 (MQ=255)
        ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTgg                                                                                                                                         >  3:767253/1‑149 (MQ=255)
        ccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTgg                                                                                                                                         >  2:231674/1‑149 (MQ=255)
         cTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGGGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCGCTgat                                                                                                                                        <  4:200760/149‑3 (MQ=255)
          tGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCGCTgata                                                                                                                                       <  1:814260/149‑4 (MQ=255)
           ggTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGa                                                                                                                                      <  1:284924/149‑1 (MQ=255)
           ggTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGa                                                                                                                                      <  1:892206/149‑1 (MQ=255)
           ggTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGa                                                                                                                                      <  3:795188/149‑1 (MQ=255)
               tACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGcat                                                                                                                                  >  3:507821/1‑149 (MQ=255)
               tACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGcat                                                                                                                                  >  1:42866/1‑149 (MQ=255)
                  acCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGcatcat                                                                                                                               >  4:873141/1‑149 (MQ=255)
                   ccTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCatcatc                                                                                                                              <  2:212956/149‑1 (MQ=255)
                   ccTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGCCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCatcatc                                                                                                                              <  4:864269/149‑1 (MQ=255)
                    cTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCg                                                                                                                             >  2:308424/1‑149 (MQ=255)
                    cTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCg                                                                                                                             >  3:299305/1‑149 (MQ=255)
                    cTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCg                                                                                                                             >  1:146655/1‑149 (MQ=255)
                     ttCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCgg                                                                                                                            >  2:439384/1‑149 (MQ=255)
                         ttAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaaa                                                                                                                        >  3:298319/1‑149 (MQ=255)
                         ttAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCaaa                                                                                                                        >  4:182650/1‑149 (MQ=255)
                              gTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                                   <  2:679367/149‑1 (MQ=255)
                              gTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                                   <  1:639215/149‑1 (MQ=255)
                              gTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAATCCTGGCGCTTCTTCCCGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGa                                                                                                                   >  2:388611/1‑149 (MQ=255)
                                 ccGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGa                                                                                                                <  1:231674/149‑1 (MQ=255)
                                 ccGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGa                                                                                                                <  3:692487/149‑1 (MQ=255)
                                   gTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGc                                                                                                              >  3:251633/1‑149 (MQ=255)
                                     aCTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAg                                                                                                            <  3:873141/149‑1 (MQ=255)
                                         cgcCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTgg                                                                                                        <  4:589560/149‑1 (MQ=255)
                                                 cGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTc                                                                                                <  2:62340/149‑1 (MQ=255)
                                                      gTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGgcgc                                                                                           >  4:504700/1‑149 (MQ=255)
                                                       tcatcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGcc                                                                                          <  1:500528/149‑1 (MQ=255)
                                                          tcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGTCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtg                                                                                       >  4:510739/1‑149 (MQ=255)
                                                           caCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgt                                                                                      <  2:80643/149‑1 (MQ=255)
                                                           caCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgt                                                                                      <  1:720119/149‑1 (MQ=255)
                                                            aCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgtg                                                                                     >  1:799053/1‑149 (MQ=255)
                                                                   aCCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTg                                                                              <  3:257079/149‑1 (MQ=255)
                                                                     ccGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTggg                                                                            >  1:78632/1‑149 (MQ=255)
                                                                       gAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGc                                                                          <  1:160799/149‑1 (MQ=255)
                                                                                 cACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgt                                                                >  1:676067/1‑149 (MQ=255)
                                                                                    gCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggt                                                             <  1:804777/149‑1 (MQ=255)
                                                                                        tGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGCGCGGCGTGGtgctg                                                         <  3:67183/149‑1 (MQ=255)
                                                                                            gTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGctct                                                     <  3:641669/149‑1 (MQ=255)
                                                                                             tCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTa                                                    <  4:821315/149‑1 (MQ=255)
                                                                                              ccTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTAc                                                   <  4:751894/149‑1 (MQ=255)
                                                                                              ccTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTAc                                                   <  2:676067/149‑1 (MQ=255)
                                                                                                  gcgcTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCt                                               <  4:394067/149‑1 (MQ=255)
                                                                                                    gcTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGc                                             >  4:407355/1‑149 (MQ=255)
                                                                                                      ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGctt                                           >  3:193293/1‑149 (MQ=255)
                                                                                                       tcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGcttc                                          >  1:313760/1‑149 (MQ=255)
                                                                                                       tcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGcttc                                          <  1:308424/149‑1 (MQ=255)
                                                                                                         ttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTAGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGcttctt                                        <  3:232838/149‑1 (MQ=255)
                                                                                                             tGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCCTCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCCCg                                    >  4:20141/1‑149 (MQ=255)
                                                                                                             tGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACg                                    >  1:884497/1‑149 (MQ=255)
                                                                                                              ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGa                                   <  4:767253/149‑1 (MQ=255)
                                                                                                               gcggcggcgGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGAc                                  >  2:391956/1‑149 (MQ=255)
                                                                                                                 ggcggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCa                                >  1:565745/1‑149 (MQ=255)
                                                                                                                   cggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCaaa                              <  2:78632/149‑1 (MQ=255)
                                                                                                                     gcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAaga                            >  2:589128/1‑149 (MQ=255)
                                                                                                                       gggCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgc                          <  2:799053/149‑1 (MQ=255)
                                                                                                                         gctcgctcGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGt                                                                                                          >  2:674780/1‑67 (MQ=255)
                                                                                                                         gctcgctcGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGt                                                                                                          <  1:674780/67‑1 (MQ=255)
                                                                                                                         gctcgctcGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgcgc                        <  3:510739/149‑1 (MQ=255)
                                                                                                                         gctcgctcGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAgcgc                        >  3:297842/1‑149 (MQ=255)
                                                                                                                             gctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGc                                             >  1:184426/1‑124 (MQ=255)
                                                                                                                             gctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGc                                             <  2:184426/124‑1 (MQ=255)
                                                                                                                             gctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTaca                    >  3:811346/1‑149 (MQ=255)
                                                                                                                             gctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTaca                    >  2:309083/1‑149 (MQ=255)
                                                                                                                             gctcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTaca                    >  2:296576/1‑149 (MQ=255)
                                                                                                                               tcgctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACAcc                  >  1:244502/1‑149 (MQ=255)
                                                                                                                                  ctATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCttt               <  1:723694/149‑1 (MQ=255)
                                                                                                                                    aTCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGa             <  3:496119/149‑1 (MQ=255)
                                                                                                                                     tCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAg            >  2:17386/1‑149 (MQ=255)
                                                                                                                                     tCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCGTCTTCACGACCAAAGAGCGCTACACCTTTGAg            <  3:520276/149‑1 (MQ=255)
                                                                                                                                     tCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGACGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAg            <  2:146655/149‑1 (MQ=255)
                                                                                                                                      cAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAgg           >  2:420439/1‑149 (MQ=255)
                                                                                                                                      cAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAgg           >  3:178489/1‑149 (MQ=255)
                                                                                                                                       aGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGt          <  4:507821/149‑1 (MQ=255)
                                                                                                                                       aGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGt          <  1:296576/149‑1 (MQ=255)
                                                                                                                                        gCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTg         >  2:595522/1‑149 (MQ=255)
                                                                                                                                        gCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTg         >  3:427327/1‑149 (MQ=255)
                                                                                                                                           gCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAg      >  4:255363/1‑149 (MQ=255)
                                                                                                                                             aTCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGcc    <  2:244502/149‑1 (MQ=255)
                                                                                                                                               cgcgCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCgg  <  4:95990/149‑1 (MQ=255)
                                                                                                                                                  gcTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCttcttc                                       >  4:406618/1‑109 (MQ=255)
                                                                                                                                                  gcTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCttcttc                                       <  3:406618/109‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGG  >  NC_000913/285740‑286031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: