Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,672,639 A→C 6.9% Q198P (CAG→CCG) ‡ hcaD → phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,672,6390AC 6.9% 333.4 / 15.9 101Q198P (CAG→CCG) ‡hcaDphenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base A (48/46);  new base C (3/4);  total (51/50)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

Only 100 of 103 total aligned reads displayed.

ATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTC  >  NC_000913/2672491‑2672785
                                                                                                                                                     |                                                                                                                                                  
aTTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACcagca                                                                                                                                                    <  3:82906/149‑1 (MQ=255)
  tATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAgg                                                                                                                                                  <  2:728972/149‑1 (MQ=255)
         gCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCCTGCTGgtgg                                                                                                                                           <  4:150828/149‑2 (MQ=255)
            ggAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCACACCAGCCTGCTGgtggcg                                                                                                                                         <  4:449278/149‑4 (MQ=255)
              aaCTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTCTACAGCGCCACCAGCAGGCTGGTGTGCGCa                                                                                                                                      <  1:514118/149‑1 (MQ=255)
               aCTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCAt                                                                                                                                     >  3:682172/1‑149 (MQ=255)
                cTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCAtt                                                                                                                                    >  1:347146/1‑149 (MQ=255)
                 tATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTc                                                                                                                                   >  1:801731/1‑149 (MQ=255)
                  aTTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCt                                                                                                                                  <  1:834810/149‑1 (MQ=255)
                   ttGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTg                                                                                                                                 >  3:794274/1‑149 (MQ=255)
                       tCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCa                                                                                                                             >  4:207688/1‑149 (MQ=255)
                        cTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATTCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaa                                                                                                                            <  2:284472/149‑1 (MQ=255)
                         tGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaat                                                                                                                           >  1:289297/1‑149 (MQ=255)
                         tGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaat                                                                                                                           >  1:706636/1‑149 (MQ=255)
                         tGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGAGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGCTGTGCGCATTCTGCTCaat                                                                                                                           >  4:92801/1‑149 (MQ=255)
                           gAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaataa                                                                                                                         >  2:420486/1‑149 (MQ=255)
                           gAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaataa                                                                                                                         >  2:546741/1‑149 (MQ=255)
                           gAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaataa                                                                                                                         >  3:16002/1‑149 (MQ=255)
                               tGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGcc                                                                                                                     >  4:697484/1‑149 (MQ=255)
                                 gCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCAt                                                                                                                   <  1:756490/149‑1 (MQ=255)
                                     ccAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGaa                                                                                                               >  2:796479/1‑149 (MQ=255)
                                     ccAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGaa                                                                                                               <  4:347879/149‑1 (MQ=255)
                                     ccAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGaa                                                                                                               <  2:4347/149‑1 (MQ=255)
                                           ccACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACAtgtg                                                                                                         >  1:184338/1‑149 (MQ=255)
                                            cACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTgg                                                                                                        >  4:191875/1‑149 (MQ=255)
                                            cACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTgg                                                                                                        >  4:643674/1‑149 (MQ=255)
                                              cgcagcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTc                                                                                                      <  4:794274/149‑1 (MQ=255)
                                                cagcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGa                                                                                                    <  1:483289/149‑1 (MQ=255)
                                                 agcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGAt                                                                                                   <  2:555731/149‑1 (MQ=255)
                                                 agcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGAt                                                                                                   <  3:86350/149‑1 (MQ=255)
                                                   cgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATgg                                                                                                 <  2:289297/149‑1 (MQ=255)
                                                    gcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGt                                                                                                >  1:504317/1‑149 (MQ=255)
                                                     cagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTg                                                                                               <  4:191779/149‑1 (MQ=255)
                                                        aTGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGaaa                                                                                            >  4:522976/1‑149 (MQ=255)
                                                          gTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCGACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGaaaaa                                                                                          <  4:559037/149‑1 (MQ=255)
                                                            aaGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGt                                                                                        >  1:374161/1‑149 (MQ=255)
                                                             aGGTGATAGTGATTGACCTGGCGGCAACCGTCATGGGCCGTCATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCCGCAGGCTGGTGTGCGAATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAGAAGTa                                                                                       <  4:156818/149‑1 (MQ=255)
                                                             aGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGc                                                                                                                                >  2:391918/1‑108 (MQ=255)
                                                             aGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGc                                                                                                                                <  1:391918/108‑1 (MQ=255)
                                                             aGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTa                                                                                       <  2:801731/149‑1 (MQ=255)
                                                             aGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCATGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTa                                                                                       <  2:252656/149‑1 (MQ=255)
                                                               gTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGa                                                                                     >  1:829273/1‑149 (MQ=255)
                                                                 gACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAAc                                                                                   <  3:196368/149‑1 (MQ=255)
                                                                 gACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAAc                                                                                   <  3:161480/149‑1 (MQ=255)
                                                                    aGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGa                                                                                >  2:106163/1‑149 (MQ=255)
                                                                       gATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGc                                                                             <  3:655953/149‑1 (MQ=255)
                                                                         ttGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTg                                                                           >  4:669865/1‑149 (MQ=255)
                                                                             aCTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCaaa                                                                       >  1:305566/1‑149 (MQ=255)
                                                                             aCTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCaaa                                                                       <  4:447758/149‑1 (MQ=255)
                                                                               tGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGt                                                                     <  1:546741/149‑1 (MQ=255)
                                                                                ggcggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTg                                                                    <  3:218204/149‑1 (MQ=255)
                                                                                 gcggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTgg                                                                   >  1:533615/1‑149 (MQ=255)
                                                                                  cggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTggg                                                                  <  1:219554/149‑1 (MQ=255)
                                                                                      aaCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGaaa                                                              >  1:108500/1‑149 (MQ=255)
                                                                                      aaCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGaaa                                                              >  3:141252/1‑149 (MQ=255)
                                                                                         cGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGaaa                                                                                            <  2:606289/116‑1 (MQ=255)
                                                                                         cGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGaaa                                                                                            >  1:606289/1‑116 (MQ=255)
                                                                                             atcggCCGGAATGCACCACCGCGCGAGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTTGTGTGCGCCTTCTGCTCAGTAATGCCGTGGAACATGTGGGCGATGGTGAAAAGGTAGAACGGAGGCTGCTAGGAGGTGAAAGGCttta                                                       >  4:685274/4‑147 (MQ=255)
                                                                                             aTGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCa                                                       >  3:813771/1‑149 (MQ=255)
                                                                                             aTGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCa                                                       <  1:420486/149‑1 (MQ=255)
                                                                                             aTGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCa                                                       <  1:443911/149‑1 (MQ=255)
                                                                                             aTGGGCCGTAATGCACCACCGCCCGTGCAACGCTACCTTCTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGTCGCTGCAAAGTGGGGAAACGCTTCa                                                       <  2:675509/149‑1 (MQ=255)
                                                                                               gggCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAgg                                                     >  2:406847/1‑149 (MQ=255)
                                                                                                 gCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCt                                                   <  4:525569/149‑1 (MQ=255)
                                                                                                 gCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCt                                                   >  3:249650/1‑149 (MQ=255)
                                                                                                    gTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGAt                                                >  3:8125/1‑149 (MQ=255)
                                                                                                        tGCACCACCGGCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtgg                                            <  1:538594/149‑1 (MQ=255)
                                                                                                        tGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtgg                                            <  2:41922/149‑1 (MQ=255)
                                                                                                        tGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtgg                                            <  2:376226/149‑1 (MQ=255)
                                                                                                         gCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtggt                                           <  4:229805/149‑1 (MQ=255)
                                                                                                           accaccGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGa                                         >  3:405194/1‑149 (MQ=255)
                                                                                                            ccaccGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAt                                        <  1:796479/149‑1 (MQ=255)
                                                                                                             caccGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAtt                                       >  1:433132/1‑149 (MQ=255)
                                                                                                              accGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAttt                                      >  1:146234/1‑149 (MQ=255)
                                                                                                              accGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAttt                                      >  1:560934/1‑149 (MQ=255)
                                                                                                                cGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTAc                                    <  3:669865/149‑1 (MQ=255)
                                                                                                                 gCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACg                                   <  4:405194/149‑1 (MQ=255)
                                                                                                                     gTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTAt                               <  1:58413/149‑1 (MQ=255)
                                                                                                                      tGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTAtt                              >  1:670879/1‑149 (MQ=255)
                                                                                                                       gCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTg                             <  2:533615/149‑1 (MQ=255)
                                                                                                                         aaCGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACg                                   >  2:368420/1‑141 (MQ=255)
                                                                                                                         aaCGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACg                                   <  1:368420/141‑1 (MQ=255)
                                                                                                                         aaCGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGt                           >  2:829491/1‑149 (MQ=255)
                                                                                                                         aaCGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGt                           >  3:800376/1‑149 (MQ=255)
                                                                                                                          aCGCTATCTTTTGCAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTAACGGTATTGGTa                          <  2:132714/149‑1 (MQ=255)
                                                                                                                                tCTTTTACAGCGCCACCAGCAGGCCGGTGTGCGCATGCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAgcg                    >  4:228992/1‑149 (MQ=255)
                                                                                                                                  ttttACAGCGCCACCAGCAGGCTGGTGTGTGAATTCTGCTCAATAATGCCATTGAAAATGTCGACGATTGTGAAAAAGTAGAACTGACACCGCAAAGGGGCGCAACGCTTAAAGCTGATGTGGTGCTTTAAGGCATTCGTATCAGCGcc                  >  1:105887/1‑149 (MQ=255)
                                                                                                                                         gcgcCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGc           >  2:671212/1‑149 (MQ=255)
                                                                                                                                         gcgcCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGc           >  1:227021/1‑149 (MQ=255)
                                                                                                                                           gcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTg                                                                    <  3:47836/88‑1 (MQ=255)
                                                                                                                                           gcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTg                                                                    >  4:47836/4‑91 (MQ=255)
                                                                                                                                           gcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGa                                         <  1:383451/115‑1 (MQ=255)
                                                                                                                                           gcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGa                                         >  2:383451/4‑118 (MQ=255)
                                                                                                                                             acaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAAc        >  2:261995/2‑149 (MQ=255)
                                                                                                                                              caccaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACt       >  3:507916/1‑149 (MQ=255)
                                                                                                                                               accaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGc                                                    <  3:256771/103‑1 (MQ=255)
                                                                                                                                               accaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGc                                                    >  4:256771/1‑103 (MQ=255)
                                                                                                                                                ccaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTgg     <  4:698970/149‑1 (MQ=255)
                                                                                                                                                ccaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGCTCGATGGTGAAAAAGTAGAACTGACCCTGCAAAGCGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTgg     >  4:620868/1‑149 (MQ=255)
                                                                                                                                                   gcagGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTc  <  3:272455/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTC  >  NC_000913/2672491‑2672785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: