Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,681,969 C→G 5.2% Q259H (CAG→CAC) ‡ yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,681,9690CG 5.2% 406.1 / 8.5 116Q259H (CAG→CAC) ‡yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base C (56/54);  new base G (2/4);  total (58/58)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 117 total aligned reads displayed.

CGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACA  >  NC_000913/2681821‑2682111
                                                                                                                                                    |                                                                                                                                              
cGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTAc                                                                                                                                                <  4:671808/149‑1 (MQ=255)
  ttATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTg                                                                                                                                              >  3:864802/1‑149 (MQ=255)
   tATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGc                                                                                                                                             <  2:67051/149‑1 (MQ=255)
    aTTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCg                                                                                                                                            <  4:691125/149‑1 (MQ=255)
       gTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAtt                                                                                                                                         <  4:326204/149‑1 (MQ=255)
       gTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGCAGTACGAtt                                                                                                                                         <  1:164731/149‑1 (MQ=255)
          ggTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAttttt                                                                                                                                      >  3:731946/1‑149 (MQ=255)
           gTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGAAAAATCGCAGTACGAttttgtc                                                                                                                                    <  2:517929/149‑4 (MQ=255)
            tCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCg                                                                                                                                    <  2:56403/149‑1 (MQ=255)
            tCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCg                                                                                                                                    <  1:817507/149‑1 (MQ=255)
            tCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCg                                                                                                                                    >  1:795729/1‑149 (MQ=255)
                aCTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCa                                                                                                                                >  4:784067/1‑149 (MQ=255)
                aCTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCa                                                                                                                                >  2:352851/1‑149 (MQ=255)
                 ctctTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCat                                                                                                                               <  2:624711/149‑1 (MQ=255)
                  tctTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCata                                                                                                                              >  3:432332/1‑149 (MQ=255)
                  tctTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCata                                                                                                                              >  4:438053/1‑149 (MQ=255)
                  actTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCata                                                                                                                              >  1:170863/2‑149 (MQ=255)
                   ctTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatat                                                                                                                             <  4:64073/149‑1 (MQ=255)
                    ttATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCata                                                                                                                              >  4:797582/1‑147 (MQ=255)
                      aTCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATg                                                                                                                          >  1:620210/1‑149 (MQ=255)
                      aTCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATg                                                                                                                          >  1:806851/1‑149 (MQ=255)
                       tCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCATCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGa                                                                                                                         >  1:47798/1‑149 (MQ=255)
                        cccACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAg                                                                                                                        <  2:461153/149‑1 (MQ=255)
                          cACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGt                                                                                                                      >  1:648733/1‑149 (MQ=255)
                           gcGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACGCCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGtt                                                                                                                     <  3:89052/148‑1 (MQ=255)
                           actccTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACGTGGCGCAATATGGTGGTTGGCAACTTGCAGCAAACCGCCATCTTCATCGTAACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGtt                                                                                                                     <  2:441023/146‑1 (MQ=255)
                           aCGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGtt                                                                                                                     >  3:218637/1‑149 (MQ=255)
                           aCGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGtt                                                                                                                     <  1:274270/149‑1 (MQ=255)
                             gacTGGCCAAATTCACTGTGTCCCCAACACCACTGTTTTTTACCTGGCGCAATATTATGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACGACGCGCCCACAAAATCGTACTCCGATTTTTCGGCCATATATGAAGTTgg                                                                                                                   <  1:172400/147‑1 (MQ=255)
                             gCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTgg                                                                                                                   <  3:688882/149‑1 (MQ=255)
                               cTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGaa                                                                                                                 <  4:218637/149‑1 (MQ=255)
                               cTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGaa                                                                                                                 <  1:308101/149‑1 (MQ=255)
                               cTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGaa                                                                                                                 <  4:731946/149‑1 (MQ=255)
                                   ccAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGAAAAATCGCAGTACGAttttgtc                                                                                                                                    >  4:361777/1‑122 (MQ=255)
                                   ccAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGAAAAATCGCAGTACGAttttgtc                                                                                                                                    <  3:361777/125‑4 (MQ=255)
                                     aaaTTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGca                                                                                                           >  1:233723/1‑149 (MQ=255)
                                       aTTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGCAGTACGAtttt                                                                                                                                       <  4:483453/119‑1 (MQ=255)
                                             tgtgTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTta                                                                                                   <  3:784067/149‑1 (MQ=255)
                                             tgtgTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTta                                                                                                   >  2:330277/1‑149 (MQ=255)
                                               tgtCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTtata                                                                                                 >  3:844628/1‑149 (MQ=255)
                                                 tCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGc                                                                                               <  3:484077/149‑1 (MQ=255)
                                                   cccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCga                                                                                             >  4:744096/1‑149 (MQ=255)
                                                   cccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCga                                                                                             >  2:589554/1‑149 (MQ=255)
                                                   cccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCga                                                                                             <  3:744096/149‑1 (MQ=255)
                                                   cccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCga                                                                                             >  2:31114/1‑149 (MQ=255)
                                                    ccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTGTAGCgag                                                                                            >  4:168781/1‑149 (MQ=255)
                                                    ccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgag                                                                                            <  3:472793/149‑1 (MQ=255)
                                                    ccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgag                                                                                            >  3:671866/1‑149 (MQ=255)
                                                    ccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgag                                                                                            >  3:809241/1‑149 (MQ=255)
                                                    ccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgag                                                                                            >  4:582458/1‑149 (MQ=255)
                                                    ccAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGACCATATATGAGGTTGGAACAGGCACATTTTTATATCgag                                                                                            >  4:551068/1‑149 (MQ=255)
                                                      aaCTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGaa                                                                                          >  1:1573/1‑149 (MQ=255)
                                                       aCTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGaaa                                                                                         <  3:14408/149‑1 (MQ=255)
                                                           cACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTc                                                                                     <  3:452072/149‑1 (MQ=255)
                                                            aCTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTcc                                                                                    >  1:170176/1‑149 (MQ=255)
                                                              tGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTc                                                                                     <  3:852560/146‑1 (MQ=255)
                                                              tGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTc                                                                                     >  4:852560/1‑146 (MQ=255)
                                                              tGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCAc                                                                                  >  2:349126/1‑149 (MQ=255)
                                                              tGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCAc                                                                                  >  3:422070/1‑149 (MQ=255)
                                                              tGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCAc                                                                                  >  1:643593/1‑149 (MQ=255)
                                                                  ttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATCGCGAGAAATGTCCACTCCa                                                                              <  3:695013/149‑1 (MQ=255)
                                                                  ttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCa                                                                              <  4:855600/149‑1 (MQ=255)
                                                                  ttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCa                                                                              <  3:163435/149‑1 (MQ=255)
                                                                      aCCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCgg                                                                          <  4:517477/149‑1 (MQ=255)
                                                                        cTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGa                                                                         <  3:20871/148‑1 (MQ=255)
                                                                        cTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGa                                                                         >  4:20871/1‑148 (MQ=255)
                                                                             gcAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTc                                                                   <  2:456170/149‑1 (MQ=255)
                                                                                     gtggtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTgt                                                           <  4:671866/149‑1 (MQ=255)
                                                                                      tggtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTgta                                                          >  3:328189/1‑149 (MQ=255)
                                                                                      tggtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTgta                                                          <  1:708904/149‑1 (MQ=255)
                                                                                          tGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTaa                                                      <  1:330277/149‑1 (MQ=255)
                                                                                           ggtaaCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAg                                                     >  4:159391/4‑149 (MQ=255)
                                                                                            gCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt                                                    >  1:359273/1‑149 (MQ=255)
                                                                                            gCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt                                                    >  1:38717/1‑149 (MQ=255)
                                                                                              aaCGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTGTGTAGTAAGTGc                                                  >  1:70412/1‑149 (MQ=255)
                                                                                                 gTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCgg                                               <  4:460720/149‑1 (MQ=255)
                                                                                                     agcaAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGc                                           <  3:87498/149‑1 (MQ=255)
                                                                                                          aCCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATgg                                      <  1:274665/149‑1 (MQ=255)
                                                                                                             gCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGa                                   >  3:775564/1‑149 (MQ=255)
                                                                                                                aTCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAg                                >  1:673256/1‑149 (MQ=255)
                                                                                                                  cTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATggg                                     >  2:778032/1‑142 (MQ=255)
                                                                                                                   ttCATCGTGACACCACGCGCCGACAAAATCGTGCTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGATAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCgg                             <  3:551068/149‑1 (MQ=255)
                                                                                                                     cATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGgag                           <  2:795729/149‑1 (MQ=255)
                                                                                                                      aTCGTGACACCCCGCGCCGACAAATTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGTGAAATGTCCACTCCAGCGGAGTAGTCCCCTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGgaga                          <  2:346764/149‑1 (MQ=255)
                                                                                                                      aTCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATgg                                      <  3:738035/137‑1 (MQ=255)
                                                                                                                      aTCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGgaga                          <  1:401856/149‑1 (MQ=255)
                                                                                                                                cacGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGttt                <  4:706057/149‑1 (MQ=255)
                                                                                                                                 acGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGga                            >  1:11755/1‑136 (MQ=255)
                                                                                                                                 acGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGga                            <  2:11755/136‑1 (MQ=255)
                                                                                                                                 acGCGCCGACAAAATCGTACTGCGATTTTTCCGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTg               >  2:857955/1‑149 (MQ=255)
                                                                                                                                           aaaaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCaa     >  1:820068/1‑149 (MQ=255)
                                                                                                                                           aaaaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCaa     >  2:336444/1‑149 (MQ=255)
                                                                                                                                           aaaaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCaa     >  3:653298/1‑149 (MQ=255)
                                                                                                                                            aaaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCaaa    <  1:352851/149‑1 (MQ=255)
                                                                                                                                             aaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGga                            >  1:95918/1‑124 (MQ=255)
                                                                                                                                             aaTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGga                            <  2:95918/124‑1 (MQ=255)
                                                                                                                                              aTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAaca  >  1:586416/1‑149 (MQ=255)
                                                                                                                                              aTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAaca  <  2:806851/149‑1 (MQ=255)
                                                                                                                                              aTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAaca  <  1:31114/149‑1 (MQ=255)
                                                                                                                                              aTCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAaca  >  3:782672/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACA  >  NC_000913/2681821‑2682111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: