Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,794,095 C→T 6.1% intergenic (+80/‑158) gabT → / → gabP 4‑aminobutyrate aminotransferase, PLP‑dependent/gamma‑aminobutyrate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,794,0950CT 6.1% 322.9 / 8.2 98intergenic (+80/‑158)gabT/gabP4‑aminobutyrate aminotransferase, PLP‑dependent/gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base C (48/44);  new base T (2/4);  total (50/48)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 102 total aligned reads displayed.

ATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGACACACTTTCGAGA  >  NC_000913/2793947‑2794243
                                                                                                                                                    |                                                                                                                                                    
aTTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcgccgc                                                                                                                                                      <  4:744846/149‑1 (MQ=255)
aTTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTAcgccgc                                                                                                                                                      >  3:91343/1‑149 (MQ=255)
    aaGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                                  >  1:528296/1‑149 (MQ=255)
      gACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATGCGGCGTAAACGCCTTATCCGa                                                                                                                                                <  4:351366/149‑1 (MQ=255)
      gACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGa                                                                                                                                                >  4:566532/1‑149 (MQ=255)
      gACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGa                                                                                                                                                >  3:867562/1‑149 (MQ=255)
       aCGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGAc                                                                                                                                               <  2:307854/149‑1 (MQ=255)
       aCGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTCTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGAc                                                                                                                                               <  2:264886/149‑1 (MQ=255)
        cGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                              <  2:655601/149‑2 (MQ=255)
        cGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                                                                                              <  3:59431/149‑2 (MQ=255)
         gCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACaa                                                                                                                                             <  4:867562/149‑1 (MQ=255)
         gCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACaa                                                                                                                                             >  1:653618/1‑149 (MQ=255)
           tCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                                                                                                                           >  1:476891/1‑149 (MQ=255)
           tCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                                                                                                                           >  2:202787/1‑149 (MQ=255)
           tCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                                                                                                                           >  2:370500/1‑149 (MQ=255)
           tCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                                                                                                                                           <  3:96791/149‑1 (MQ=255)
               aTCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTa                                                                                                                                       >  4:695921/1‑149 (MQ=255)
                 ccGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCGTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACg                                                                                                                                     >  2:751664/1‑149 (MQ=255)
                 ccGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACg                                                                                                                                     >  1:699105/1‑149 (MQ=255)
                  cGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGt                                                                                                                                    <  4:42602/149‑1 (MQ=255)
                      aGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc                                                                                                                                >  3:237147/1‑149 (MQ=255)
                      aGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc                                                                                                                                <  4:178981/149‑1 (MQ=255)
                       gggTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACa                                                                                                                               >  1:836184/1‑149 (MQ=255)
                           cTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                                                                                                                           <  2:836184/149‑1 (MQ=255)
                           cTGGAGATCAGCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTACGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGCATTCAGGTCAGATAAGGCGTTTACGCCGCATCCGGCAATCTGTACGTGAACAGgaa                                                                                                                           <  2:862357/149‑1 (MQ=255)
                            tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTg                                                                                                                                   >  1:406622/1‑140 (MQ=255)
                            tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTg                                                                                                                                   <  2:406622/140‑1 (MQ=255)
                            tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaag                                                                                                                          <  3:213720/149‑1 (MQ=255)
                            tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaag                                                                                                                          >  2:230949/1‑149 (MQ=255)
                               agaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGaaa                                                                                                                       <  2:782051/149‑1 (MQ=255)
                               agaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGaaa                                                                                                                       <  2:136981/149‑1 (MQ=255)
                               agaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGaaa                                                                                                                       >  1:706849/1‑149 (MQ=255)
                                 atcatcAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATc                                                                                                                     >  2:872851/1‑149 (MQ=255)
                                    atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTAt                                                                                                                  <  4:259574/149‑1 (MQ=255)
                                    atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTAt                                                                                                                  <  2:848187/149‑1 (MQ=255)
                                    atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTAt                                                                                                                  <  3:429214/149‑1 (MQ=255)
                                     tcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATg                                                                                                                 <  1:503042/149‑1 (MQ=255)
                                      caGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGt                                                                                                                >  4:336162/1‑149 (MQ=255)
                                           aGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAAt                                                                                                                      <  2:340568/138‑1 (MQ=255)
                                           aGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAAt                                                                                                                      >  1:340568/1‑138 (MQ=255)
                                             tgtTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCgg                                                                                                         >  1:548584/1‑149 (MQ=255)
                                              gtTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCggg                                                                                                        <  1:872851/149‑1 (MQ=255)
                                               ttttGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGt                                                                                                       >  4:253638/1‑149 (MQ=255)
                                                   gatgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTg                                                                                                              <  3:155054/138‑1 (MQ=255)
                                                   gatgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTg                                                                                                              >  4:155054/1‑138 (MQ=255)
                                                    atgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGc                                                                                                  <  3:573571/149‑1 (MQ=255)
                                                    atgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGc                                                                                                  >  1:79500/1‑149 (MQ=255)
                                                    atgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGc                                                                                                  <  1:550346/149‑1 (MQ=255)
                                                        ggCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAg                                                                                              >  1:502491/1‑149 (MQ=255)
                                                         gCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGc                                                                                             >  2:836558/1‑149 (MQ=255)
                                                           gAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATATGTCCGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCg                                                                                           >  3:94301/1‑149 (MQ=255)
                                                              gCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGctc                                                                                        <  1:230949/149‑1 (MQ=255)
                                                                 gTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTcc                                                                                     >  2:369920/1‑149 (MQ=255)
                                                                    gcgcCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGc                                                                                  >  4:46082/1‑149 (MQ=255)
                                                                           tCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGa                                                                           >  2:275178/1‑149 (MQ=255)
                                                                            ccTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGaa                                                                          <  2:354112/149‑1 (MQ=255)
                                                                            ccTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGaa                                                                          <  2:116704/149‑1 (MQ=255)
                                                                            ccTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGaa                                                                          <  4:259361/149‑1 (MQ=255)
                                                                             cTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAAt                                                                         <  3:703664/149‑1 (MQ=255)
                                                                             cTACGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTGCGGGGATCGAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGCACGTGAACAGGAAGAAAACTATGTTGGCCGGGTAAGGCGGAGCCGCTTTCCGGCAAAAAGAAt                                                                         <  2:383394/149‑1 (MQ=255)
                                                                                  ccGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATa                                                                    >  3:533717/1‑149 (MQ=255)
                                                                                  ccGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATa                                                                    <  1:61283/149‑1 (MQ=255)
                                                                                   cGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATaa                                                                   <  1:461782/149‑1 (MQ=255)
                                                                                   cGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATaa                                                                   <  2:348334/149‑1 (MQ=255)
                                                                                      aGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACaa                                                                <  1:244582/149‑1 (MQ=255)
                                                                                      aGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACaa                                                                <  4:878767/149‑1 (MQ=255)
                                                                                         cGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATta                                                             <  3:253638/149‑1 (MQ=255)
                                                                                          gACGCTGCGCGTCTTGTCCGGTCTACGGGGATCCAGGTCGGGTAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATtat                                                            >  2:150283/1‑149 (MQ=255)
                                                                                          gACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATtat                                                            >  3:879138/1‑149 (MQ=255)
                                                                                            cGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATAc                                                          >  4:661176/1‑149 (MQ=255)
                                                                                                     tCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGAccc                                                 >  3:42947/1‑149 (MQ=255)
                                                                                                          tCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATc                                                                        >  1:743030/1‑121 (MQ=255)
                                                                                                          tCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATc                                                                        <  2:743030/121‑1 (MQ=255)
                                                                                                                    ggggATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTc                                  >  1:77574/1‑149 (MQ=255)
                                                                                                                      ggATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCgg                                <  3:695921/149‑1 (MQ=255)
                                                                                                                        aTCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCgggg                              >  1:21157/1‑149 (MQ=255)
                                                                                                                           cAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGgcgc                           <  1:369920/149‑1 (MQ=255)
                                                                                                                           attgTCGGATGCGGCGTAAACGCCTTATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGgcg                            >  3:741777/4‑148 (MQ=255)
                                                                                                                           attgTCGGATGCGGCGTAAACGCCTTATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGgcg                            <  4:741777/145‑1 (MQ=255)
                                                                                                                            aGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                                                                                                                           <  3:171461/52‑1 (MQ=255)
                                                                                                                            aGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa                                                                                                                           >  4:171461/1‑52 (MQ=255)
                                                                                                                            aGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGct                          >  2:534904/1‑149 (MQ=255)
                                                                                                                               tCGGATGCGGCGTAAACGCCTTATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCtctc                       >  1:700602/1‑149 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGTGAAATGTCGGGGCGCTCTCCCa                    >  3:728700/1‑149 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCa                    >  4:367680/1‑149 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCa                    >  4:626863/1‑149 (MQ=255)
                                                                                                                                      aGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTg                <  1:411838/149‑1 (MQ=255)
                                                                                                                                       ggCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGa               <  2:27701/149‑1 (MQ=255)
                                                                                                                                       ggCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGa               >  4:568308/1‑149 (MQ=255)
                                                                                                                                       ggCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCACAAGTGa               <  2:476891/149‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCtctc                       >  3:181570/1‑140 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCtctc                       <  4:181570/140‑1 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGac              >  1:460613/1‑149 (MQ=255)
                                                                                                                                        gCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGac              >  1:709939/1‑149 (MQ=255)
                                                                                                                                         cGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGaca             <  4:565243/149‑1 (MQ=255)
                                                                                                                                          gTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAGTAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGacac            >  4:758913/1‑149 (MQ=255)
                                                                                                                                           tttACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGacaca           >  1:475094/1‑149 (MQ=255)
                                                                                                                                            ttACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGacacac          <  2:653618/149‑1 (MQ=255)
                                                                                                                                                 ccgcATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGACACACTTTCg     <  4:334458/149‑1 (MQ=255)
                                                                                                                                                    cATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGACACACTTTCgaga  <  4:879138/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGACACACTTTCGAGA  >  NC_000913/2793947‑2794243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: