| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,799,636 | C→T | 9.3% | intergenic (+23/+14) | alaE → / ← ygaC | alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,799,636 | 0 | C | T | 9.3% | 252.5 / 13.0 | 87 | intergenic (+23/+14) | alaE/ygaC | alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base C (40/38); new base T (5/3); total (45/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
ATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAA > NC_000913/2799491‑2799782 | aTTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGcc > 3:740926/1‑149 (MQ=255) aTGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGt > 1:265980/1‑149 (MQ=255) tGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑GATGCCAGTc < 1:800015/149‑1 (MQ=255) tGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTc < 2:743924/149‑1 (MQ=255) tGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTc < 2:657424/149‑1 (MQ=255) aGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGAT‑GCCAGTcgc < 2:533558/140‑1 (MQ=255) aGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑GATGCCAGTcgc > 1:533558/1‑140 (MQ=255) gTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAGGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACt < 4:213319/149‑1 (MQ=255) ttCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTg > 4:200634/1‑149 (MQ=255) aaaCATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCt > 4:875554/1‑149 (MQ=255) ttgttTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCaaa < 2:776348/149‑1 (MQ=255) ttCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa < 1:549861/149‑1 (MQ=255) tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc < 2:265980/149‑1 (MQ=255) tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc < 2:258394/149‑1 (MQ=255) aTGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAACAAACCAt > 4:810715/1‑149 (MQ=255) gTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTc > 1:264319/1‑149 (MQ=255) ggggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGCCTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACCGGCTTCGCCAAATACACCACTCAAATAAc < 4:842907/149‑1 (MQ=255) ggggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAAc < 2:404003/149‑1 (MQ=255) ggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGt > 3:198078/1‑149 (MQ=255) gCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGtt < 3:563630/149‑1 (MQ=255) cGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTc < 2:725640/149‑1 (MQ=255) ggTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGCC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCa < 3:875554/149‑1 (MQ=255) gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa > 2:221816/1‑149 (MQ=255) gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa < 1:221816/149‑1 (MQ=255) ggCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCaa > 4:719829/1‑149 (MQ=255) tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGt > 1:831148/1‑136 (MQ=255) tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGt < 2:831148/136‑1 (MQ=255) tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAacac > 3:83207/1‑149 (MQ=255) aCTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACAcg > 4:198209/1‑149 (MQ=255) ccTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGaa > 3:132108/1‑149 (MQ=255) cTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAAc > 4:200618/1‑149 (MQ=255) tCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACt > 1:771287/1‑149 (MQ=255) tCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑AGCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACt > 2:637891/1‑149 (MQ=255) attattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa < 3:369223/149‑1 (MQ=255) ttattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAACGCAACACGCGAACTaaa < 1:712679/148‑1 (MQ=255) ttattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCCCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa > 2:712679/1‑148 (MQ=255) ccgccgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGc < 3:121331/149‑1 (MQ=255) cgccgACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCg > 1:40294/1‑149 (MQ=255) aCTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATa > 3:604709/1‑149 (MQ=255) tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGt > 4:532926/1‑149 (MQ=255) aaaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt < 3:381881/149‑1 (MQ=255) aaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa < 4:816448/109‑1 (MQ=255) aaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa > 3:816448/1‑109 (MQ=255) tCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTaa < 3:780181/144‑1 (MQ=255) tCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTaa > 4:780181/1‑144 (MQ=255) aGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa < 3:770635/123‑1 (MQ=255) aGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaaa > 4:770635/1‑123 (MQ=255) aGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCgt < 2:441957/149‑1 (MQ=255) aGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCgt < 3:693040/149‑1 (MQ=255) gTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcg > 2:1480/1‑149 (MQ=255) ttACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgc < 1:317358/149‑1 (MQ=255) ttACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgc < 4:746798/149‑1 (MQ=255) gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAtt < 3:276794/79‑1 (MQ=255) gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCAtt > 4:276794/1‑79 (MQ=255) gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCt > 2:217534/1‑149 (MQ=255) gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCt > 4:625600/1‑149 (MQ=255) gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCt > 3:801546/1‑149 (MQ=255) ggTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCt > 4:460600/1‑149 (MQ=255) gTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt > 2:609646/1‑128 (MQ=255) gTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt < 1:609646/128‑1 (MQ=255) gTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTa > 3:744610/1‑149 (MQ=255) aaaaGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTaaa < 4:694796/149‑1 (MQ=255) aaGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATa < 2:301877/149‑1 (MQ=255) ccTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATagag < 4:29783/149‑1 (MQ=255) cTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGc < 4:198078/149‑1 (MQ=255) aCTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGa > 2:112586/1‑149 (MQ=255) aCTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGa > 1:637798/1‑149 (MQ=255) tcttctTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGaaa > 4:454541/1‑149 (MQ=255) ttcttTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt < 4:868713/149‑1 (MQ=255) tcttTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATACCGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTg < 2:638087/149‑1 (MQ=255) tcttTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTg < 4:515747/149‑1 (MQ=255) cttTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc < 1:223890/149‑1 (MQ=255) ttttCGCGTCTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCt < 2:771287/149‑1 (MQ=255) ttttCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCt > 1:502512/1‑149 (MQ=255) ttttCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCt > 2:159198/1‑149 (MQ=255) aagacgcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCt > 3:684538/5‑149 (MQ=255) ttCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTgg < 3:200618/149‑1 (MQ=255) gacgcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCACAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc < 1:847781/144‑1 (MQ=255) gacgcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCACAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc > 2:847781/3‑146 (MQ=255) gcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgct < 1:793667/117‑1 (MQ=255) gcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGtcgct > 2:793667/1‑117 (MQ=255) gcgACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa > 1:55463/1‑149 (MQ=255) gACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTTCTGGTAATg > 1:294616/1‑149 (MQ=255) tGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc > 4:227222/1‑149 (MQ=255) tGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc < 3:719829/149‑1 (MQ=255) ggC‑ATCGCCAGTGGCGTCTTTAACTGGCTTCGTCAAATAAACCATTTAAATAAGATTCAAGAGCAACACGCGAAATAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTGATGATCg > 4:836505/1‑149 (MQ=255) aTCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCaa > 2:895943/1‑149 (MQ=255) | ATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAA > NC_000913/2799491‑2799782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |