Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,951,471 G→A 9.4% R331C (CGC→TGC)  recD ← exonuclease V (RecBCD complex), alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,951,4710GA 9.4% 312.2 / 18.0 106R331C (CGC→TGC) recDexonuclease V (RecBCD complex), alpha chain
Reads supporting (aligned to +/- strand):  ref base G (46/50);  new base A (3/7);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-02

Only 100 of 117 total aligned reads displayed.

TTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGT  >  NC_000913/2951326‑2951619
                                                                                                                                                 |                                                                                                                                                    
     accacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCt                                                                                                                                              >  4:237790/1‑149 (MQ=255)
      ccacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTg                                                                                                                                             >  1:322741/1‑149 (MQ=255)
       cacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGc                                                                                                                                            >  2:845798/1‑149 (MQ=255)
       cacGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGc                                                                                                                                            >  1:517817/1‑149 (MQ=255)
        acGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGcc                                                                                                                                           <  3:629834/149‑1 (MQ=255)
         cGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCt                                                                                                                                          <  3:857268/149‑1 (MQ=255)
           gTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTgg                                                                                                                                        >  4:786362/1‑149 (MQ=255)
           gTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCAGCTAAGCCGCCTga                                                                                                                                        <  3:131298/149‑2 (MQ=255)
           gTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCAGCTAAGCCGCCTga                                                                                                                                        <  3:18714/149‑2 (MQ=255)
             tGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacg                                                                                                                                      <  1:520853/149‑4 (MQ=255)
              gATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCGGAAACGATAGCTTTGTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                     <  1:709416/149‑5 (MQ=255)
                tCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGc                                                                                                                                   >  2:848502/1‑149 (MQ=255)
                   ccgcAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacggg                                                                                                                                    >  2:346272/1‑140 (MQ=255)
                      cAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcg                                                                                                                             <  1:454353/149‑1 (MQ=255)
                       aGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcgg                                                                                                                            <  3:7348/149‑1 (MQ=255)
                       aGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcgg                                                                                                                            <  1:482219/149‑1 (MQ=255)
                          tAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTaa                                                                                                                         >  1:580627/1‑149 (MQ=255)
                           aaCTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAggcggc                                                                                                                                                    >  3:45119/1‑121 (MQ=255)
                           aaCTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAggcggc                                                                                                                                                    <  4:45119/121‑1 (MQ=255)
                           aaCTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTaaa                                                                                                                        >  1:284416/1‑149 (MQ=255)
                             cTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGc                                                                                                                      <  1:303312/149‑1 (MQ=255)
                              tGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGcc                                                                                                                     >  4:174451/1‑149 (MQ=255)
                                  cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAggcggc                                                                                                                                                    <  2:145505/114‑1 (MQ=255)
                                  cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAggcggc                                                                                                                                                    >  1:145505/1‑114 (MQ=255)
                                  cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                     <  2:669491/129‑5 (MQ=255)
                                  cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                     <  2:48063/129‑5 (MQ=255)
                                  cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                     >  1:669491/1‑125 (MQ=255)
                                  cAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGCCAGGCAGCTAAGCCGCCTgacgg                                                                                                                                     >  1:48063/1‑125 (MQ=255)
                                      gCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCAGCGTTgg                                                                                                             <  2:479379/149‑1 (MQ=255)
                                      gCCAGAATCGCGGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTgg                                                                                                             <  2:59446/149‑1 (MQ=255)
                                       ccAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGc                                                                                                                                               >  4:426383/1‑114 (MQ=255)
                                       ccAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGc                                                                                                                                               <  3:426383/114‑1 (MQ=255)
                                           aaTCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATaa                                                                                                        <  4:302526/149‑1 (MQ=255)
                                           aaTCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATaa                                                                                                        <  1:470775/149‑1 (MQ=255)
                                             tCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAgc                                                                                                      <  1:845798/149‑1 (MQ=255)
                                              cGCTACCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAgcg                                                                                                     >  2:150309/1‑149 (MQ=255)
                                                     gAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcg                                                                                                                             <  1:596059/118‑1 (MQ=255)
                                                     gAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTcggcg                                                                                                                             >  2:596059/1‑118 (MQ=255)
                                                       aaCGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGTATAAGCGCAGATATCg                                                                                            <  1:189209/149‑1 (MQ=255)
                                                          gATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGccc                                                                                         <  2:41128/149‑1 (MQ=255)
                                                          gATAGCTGTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGccc                                                                                         >  2:477041/1‑149 (MQ=255)
                                                            tAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAg                                                                                       >  2:849454/1‑149 (MQ=255)
                                                            tAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAg                                                                                       >  1:281936/1‑149 (MQ=255)
                                                                  ttttGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCATCACAGc                                                                                 <  2:478807/149‑1 (MQ=255)
                                                                   tttGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGcc                                                                                <  3:204536/149‑1 (MQ=255)
                                                                        agcagGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGc                                                                           <  4:829846/149‑1 (MQ=255)
                                                                         gcaggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGcc                                                                          >  1:521518/1‑149 (MQ=255)
                                                                          caggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCt                                                                         <  2:749890/149‑1 (MQ=255)
                                                                               agagACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAAcc                                                                    >  2:686868/1‑149 (MQ=255)
                                                                                  gaCTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAg                                                                 <  2:322741/149‑1 (MQ=255)
                                                                                     tGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGcc                                                              >  3:844572/1‑149 (MQ=255)
                                                                                           gcAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGa                                                        <  3:402100/149‑1 (MQ=255)
                                                                                            cAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGAt                                                       <  4:684999/149‑1 (MQ=255)
                                                                                              aaGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCa                                                     >  1:197408/1‑149 (MQ=255)
                                                                                                 aTGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGa                                                  <  1:711745/149‑1 (MQ=255)
                                                                                                      gCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGcc                                             >  2:880832/1‑149 (MQ=255)
                                                                                                      gCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGcc                                             >  3:242051/1‑149 (MQ=255)
                                                                                                        ttCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCga                                           >  2:56672/1‑149 (MQ=255)
                                                                                                        ttCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCga                                           >  3:798179/1‑149 (MQ=255)
                                                                                                          cTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCgaga                                         <  3:639645/149‑1 (MQ=255)
                                                                                                            gtgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGaaa                                       <  1:364682/149‑1 (MQ=255)
                                                                                                            gtgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGaaa                                       <  1:630202/149‑1 (MQ=255)
                                                                                                            gtgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGaaa                                       <  3:288023/149‑1 (MQ=255)
                                                                                                            gtgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGaaa                                       <  4:810204/149‑1 (MQ=255)
                                                                                                             tgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAg                                      <  3:68997/149‑1 (MQ=255)
                                                                                                              tcacgTTCCGGCAGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCa                                                     >  3:741999/5‑133 (MQ=255)
                                                                                                              tcacgTTCCGGCAGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGa                                     >  2:855485/5‑149 (MQ=255)
                                                                                                              tcacgTTCCGGCAGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGa                                     >  2:227867/5‑149 (MQ=255)
                                                                                                              tcacgTTCCGGCAGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGa                                     >  4:591589/5‑149 (MQ=255)
                                                                                                              gCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACACTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGa                                     <  1:244290/149‑1 (MQ=255)
                                                                                                               ccGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGAt                                    <  2:580627/149‑1 (MQ=255)
                                                                                                                cGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATc                                   <  3:688953/149‑1 (MQ=255)
                                                                                                                 ggTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCa                                  >  1:164066/1‑149 (MQ=255)
                                                                                                                 ggTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCa                                  <  4:502268/149‑1 (MQ=255)
                                                                                                                      cTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTcgc                             >  2:287897/1‑149 (MQ=255)
                                                                                                                      cTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTcgc                             >  1:179921/1‑149 (MQ=255)
                                                                                                                       tGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTcgcg                            >  1:122728/1‑149 (MQ=255)
                                                                                                                         ccGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCa                          >  3:276021/1‑149 (MQ=255)
                                                                                                                         ccGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCa                          >  2:508776/1‑149 (MQ=255)
                                                                                                                           ggAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATg                        >  2:136455/1‑149 (MQ=255)
                                                                                                                           ggAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGACCGCGTTGGCTTAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGGTCACGATCGTCGAGAAAGATCACTCGCGCATg                        >  2:50485/1‑149 (MQ=255)
                                                                                                                             aaCGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGAt                      <  4:798179/149‑1 (MQ=255)
                                                                                                                             aaCGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACCCGCGCATGAt                      <  4:156185/149‑1 (MQ=255)
                                                                                                                              aCGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATc                     <  3:129637/149‑1 (MQ=255)
                                                                                                                                gTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCgg                   >  3:805913/1‑149 (MQ=255)
                                                                                                                                gTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGACGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCgg                   <  4:842896/149‑1 (MQ=255)
                                                                                                                                  gAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGc                 >  1:436845/1‑149 (MQ=255)
                                                                                                                                  gAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGc                 >  3:585412/1‑149 (MQ=255)
                                                                                                                                     tCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAg              <  1:428177/149‑1 (MQ=255)
                                                                                                                                       cccGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGc            <  1:880832/149‑1 (MQ=255)
                                                                                                                                          gTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTc         <  2:284416/149‑1 (MQ=255)
                                                                                                                                            cAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGa       <  1:227867/149‑1 (MQ=255)
                                                                                                                                            cAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGa       <  3:237790/149‑1 (MQ=255)
                                                                                                                                            cAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGa       <  2:164338/149‑1 (MQ=255)
                                                                                                                                              ggcggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATc     <  2:791943/149‑1 (MQ=255)
                                                                                                                                              ggcggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATc     <  2:608376/149‑1 (MQ=255)
                                                                                                                                               gcggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCa    <  1:715699/149‑1 (MQ=255)
                                                                                                                                               gcggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCa    >  1:728620/1‑149 (MQ=255)
                                                                                                                                               gcggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGAACACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCa    >  2:715699/1‑149 (MQ=255)
                                                                                                                                                 ggcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGt  <  4:156864/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTATCACCACGGTTGATCGCCGCAGCTAACTGACCAATGCCAGAATCGCTGCCGAAACGATAGCTTTTTTGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGT  >  NC_000913/2951326‑2951619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: