Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,035,480 T→C 11.0% T268A (ACC→GCC) ‡ prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,035,4800TC11.0% 269.3 / 20.2 98T268A (ACC→GCC) ‡prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (44/43);  new base C (7/4);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

TGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGA  >  NC_000913/3035333‑3035627
                                                                                                                                                   |                                                                                                                                                   
tGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTc                                                                                                                                                    <  3:374328/149‑1 (MQ=255)
 gTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCg                                                                                                                                                   <  2:895182/149‑1 (MQ=255)
  tCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCgg                                                                                                                                                  >  1:45803/1‑149 (MQ=255)
   cGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCggg                                                                                                                                                 <  4:725570/149‑1 (MQ=255)
    ggATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGTACTGGGTCACGATCCCGGTCGGGa                                                                                                                                                <  1:103932/149‑1 (MQ=255)
    ggATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGa                                                                                                                                                <  3:158419/149‑1 (MQ=255)
    ggATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGa                                                                                                                                                <  2:111650/149‑1 (MQ=255)
         tGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                                                                           <  2:45803/149‑1 (MQ=255)
         tGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                                                                           <  2:175948/149‑1 (MQ=255)
         tGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                                                                           <  4:232/149‑1 (MQ=255)
         tGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCAGAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTGTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                                                                           <  2:32761/149‑1 (MQ=255)
          gTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTggg                                                                                                                                          >  1:568241/1‑149 (MQ=255)
             aTCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTaa                                                                                                                                       <  1:721665/149‑1 (MQ=255)
               cTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCGGTTCATAAAGCTTCGCTTTCTTCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCTCGGTCGGGATGTGGGTAATa                                                                                                                                     >  2:71479/1‑149 (MQ=255)
                ttCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATAc                                                                                                                                    >  3:847909/1‑149 (MQ=255)
                    aTCGCCTGTTTCTCGGCATTTTTATCCTGGATCTCCGGTTCATAAAGCTTCGATTTCAGCTGGTTCATGGACTGGTCTTTGTTATTGTGCTGGGACCGGTCGTTTTCGCACTGGGTGACGATCCCGGTCGGGATGTGGGTAATACGCAc                                                                                                                                >  2:441428/1‑149 (MQ=255)
                       gCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGc                                                                                                                             >  3:149962/1‑149 (MQ=255)
                          tGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGa                                                                                                                          <  2:220200/149‑1 (MQ=255)
                            tttCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGAtt                                                                                                                        <  1:413586/149‑1 (MQ=255)
                                tcGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGACCGGGAtcgtg                                                                                                                                           <  2:225902/126‑5 (MQ=255)
                                tcGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGACCGGGAtcgtg                                                                                                                                           >  1:225902/1‑122 (MQ=255)
                                    cATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACgg                                                                                                                <  3:171761/149‑1 (MQ=255)
                                     aTTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGt                                                                                                               >  4:681209/1‑149 (MQ=255)
                                         ttcttcTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAAc                                                                                                           <  1:716254/149‑1 (MQ=255)
                                         ttcttcTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAAc                                                                                                           >  1:524313/1‑149 (MQ=255)
                                          tcttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACg                                                                                                          >  1:600118/1‑149 (MQ=255)
                                            ttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTg                                                                                                        >  2:379685/1‑149 (MQ=255)
                                             tctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGc                                                                                                       <  2:602776/149‑1 (MQ=255)
                                              ctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCt                                                                                                      <  4:847909/149‑1 (MQ=255)
                                                 cATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAc                                                                                                   >  1:146888/1‑149 (MQ=255)
                                                         gTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCcgcg                                                                                           >  1:853318/1‑149 (MQ=255)
                                                         gTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCcgcg                                                                                           >  1:385037/1‑149 (MQ=255)
                                                             aTAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCgg                                                                                       >  3:386092/1‑149 (MQ=255)
                                                             aTAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCgg                                                                                       >  3:332528/1‑149 (MQ=255)
                                                              tAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGa                                                                                      >  1:856519/1‑149 (MQ=255)
                                                               aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                                                                                     >  2:106545/1‑149 (MQ=255)
                                                               aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                                                                                     <  2:293669/149‑1 (MQ=255)
                                                               aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                                                                                     >  1:206617/1‑149 (MQ=255)
                                                               aaaGCTTCGCTTTCATCTGCTTCATGACCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTAAGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGat                                                                                     >  4:753654/1‑148 (MQ=255)
                                                                   cTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGc                                                                                 <  4:278086/149‑1 (MQ=255)
                                                                    ttCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCg                                                                                >  4:429670/1‑149 (MQ=255)
                                                                     tCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGa                                                                               <  2:443582/149‑1 (MQ=255)
                                                                      cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGAt                                                                              <  2:863006/149‑1 (MQ=255)
                                                                       gCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATa                                                                             <  2:175450/149‑1 (MQ=255)
                                                                         tttCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                                                                           >  2:455932/1‑149 (MQ=255)
                                                                         tttCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                                                                           >  1:452080/1‑149 (MQ=255)
                                                                         tttCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                                                                           >  4:250621/1‑149 (MQ=255)
                                                                             aTCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTc                                                                       >  1:726369/1‑149 (MQ=255)
                                                                                  cTTCATGGCCTGATCTTTGTGCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATTAACGTCAATgc                                                                  >  2:257253/1‑149 (MQ=255)
                                                                                     cATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCa                                                               <  3:743419/149‑1 (MQ=255)
                                                                                     cATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCa                                                               <  1:269276/149‑1 (MQ=255)
                                                                                          ccTGATCTTTGTTCTTTTGCTGGGACCGGACGTACTGGCACTGGGTCACGATCCCGGTGGGGAGGTGGGTAATACGCACCGAAAATTCGGTACGGTTAACGTGCTGACGGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATcc                                                          <  4:510677/149‑1 (MQ=255)
                                                                                           cTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATgc                                                                  <  4:773734/140‑1 (MQ=255)
                                                                                           cTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATgc                                                                  >  3:773734/1‑140 (MQ=255)
                                                                                            tGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATccgc                                                        >  1:255207/1‑149 (MQ=255)
                                                                                              aTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                                                                           >  4:396868/1‑128 (MQ=255)
                                                                                              aTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                                                                           <  3:396868/128‑1 (MQ=255)
                                                                                              aTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATccgccg                                                      >  4:377966/1‑149 (MQ=255)
                                                                                              aTCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATccgccg                                                      <  2:146888/149‑1 (MQ=255)
                                                                                               tCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCgg                                                     <  2:111719/149‑1 (MQ=255)
                                                                                               tCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCgg                                                     >  1:82257/1‑149 (MQ=255)
                                                                                                 tttGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTc                                                                                                                       <  4:754147/81‑1 (MQ=255)
                                                                                                 tttGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTc                                                                                                                       >  3:754147/1‑81 (MQ=255)
                                                                                                 tttGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGt                                                   >  2:801200/1‑149 (MQ=255)
                                                                                                     ttCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAt                                               >  3:749009/1‑149 (MQ=255)
                                                                                                      tCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAtt                                              <  2:112193/149‑1 (MQ=255)
                                                                                                      tCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAtt                                              <  4:209411/149‑1 (MQ=255)
                                                                                                          gtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                          <  2:524313/149‑1 (MQ=255)
                                                                                                          gtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                          <  2:520753/149‑1 (MQ=255)
                                                                                                           tgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                         >  2:433014/1‑149 (MQ=255)
                                                                                                               gggAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                     <  4:149962/149‑1 (MQ=255)
                                                                                                                ggAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaa                                    >  1:685325/1‑149 (MQ=255)
                                                                                                                  aaCGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaata                                  <  1:579209/149‑1 (MQ=255)
                                                                                                                      gTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcat                              >  1:735465/1‑149 (MQ=255)
                                                                                                                       tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatc                             >  4:236476/1‑149 (MQ=255)
                                                                                                                       tCGTTCTGGCACTGGGTCACGATCCCGGCCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCGGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatc                             <  2:238630/149‑1 (MQ=255)
                                                                                                                        cGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatca                            <  2:685325/149‑1 (MQ=255)
                                                                                                                        cGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatca                            <  2:385037/149‑1 (MQ=255)
                                                                                                                          ttCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatcatc                          >  4:510922/1‑149 (MQ=255)
                                                                                                                             tGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAAc                       <  4:613119/149‑1 (MQ=255)
                                                                                                                                 aCTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTcc                   >  4:835271/1‑149 (MQ=255)
                                                                                                                                    gggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGa                <  1:801200/149‑1 (MQ=255)
                                                                                                                                       tCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaa             >  1:892112/1‑149 (MQ=255)
                                                                                                                                        ccacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaata                                  >  4:182829/5‑127 (MQ=255)
                                                                                                                                        ccacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaata                                  <  3:182829/123‑1 (MQ=255)
                                                                                                                                        ccacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaat                                   >  4:875309/5‑126 (MQ=255)
                                                                                                                                        ccacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaat                                   <  3:875309/122‑1 (MQ=255)
                                                                                                                                        ccacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaa            >  4:437131/5‑149 (MQ=255)
                                                                                                                                        ccacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaa            >  3:559303/5‑149 (MQ=255)
                                                                                                                                        ccacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaa            >  2:677847/5‑149 (MQ=255)
                                                                                                                                         cacaTCCCGACCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaac           >  2:74057/4‑149 (MQ=255)
                                                                                                                                           gATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGt                                                   <  2:818914/107‑1 (MQ=255)
                                                                                                                                           gATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGt                                                   >  1:818914/1‑107 (MQ=255)
                                                                                                                                           gATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaa         >  4:210647/1‑149 (MQ=255)
                                                                                                                                             tCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaaac       >  3:181833/1‑149 (MQ=255)
                                                                                                                                                cGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAAcgcg    >  3:195509/1‑149 (MQ=255)
                                                                                                                                                  gTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGa  <  4:737281/149‑1 (MQ=255)
                                                                                                                                                  gTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGa  <  2:255207/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGA  >  NC_000913/3035333‑3035627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: