Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,044,330 C→G 5.4% A223A (GCC→GCG bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,044,3300CG 5.4% 318.3 / 8.1 93A223A (GCC→GCGbglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base C (39/49);  new base G (3/2);  total (42/51)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.24e-01

CCTTCAACGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGAT  >  NC_000913/3044191‑3044477
                                                                                                                                           |                                                                                                                                                   
ccTTCAACGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGGGCGCTGGCgaca                                                                                                                                            <  4:13141/149‑4 (MQ=255)
  ttCAACGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGaa                                                                                                                                          >  2:620707/1‑149 (MQ=255)
   tCAACGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGaaa                                                                                                                                         <  2:15081/149‑1 (MQ=255)
   tCAACGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGaaa                                                                                                                                         <  3:689359/149‑1 (MQ=255)
      aCGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCt                                                                                                                                      <  1:748804/149‑1 (MQ=255)
      aCGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCt                                                                                                                                      >  3:774736/1‑149 (MQ=255)
      aCGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCt                                                                                                                                      >  3:770650/1‑149 (MQ=255)
        gAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTgc                                                                                                                                    >  3:163265/1‑149 (MQ=255)
        gAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTgc                                                                                                                                    <  4:618814/149‑1 (MQ=255)
        gAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTgc                                                                                                                                    >  2:799670/1‑149 (MQ=255)
          aaTTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTgcgc                                                                                                                                  <  1:595372/149‑1 (MQ=255)
                 aacCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTCTACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCAt                                                                                                                           >  2:387393/1‑149 (MQ=255)
                 aacCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCg                                                                                                                                               >  1:616265/1‑129 (MQ=255)
                 aacCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCg                                                                                                                                               <  2:616265/129‑1 (MQ=255)
                   ccAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTa                                                                                                                         >  2:466103/1‑149 (MQ=255)
                         tAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCgg                                                                                                                   >  2:52954/1‑149 (MQ=255)
                          aaCTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGga                                                                                                                  <  4:163265/149‑1 (MQ=255)
                          aaCTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGga                                                                                                                  <  4:881296/149‑1 (MQ=255)
                          aaCTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGga                                                                                                                  <  4:488657/149‑1 (MQ=255)
                           aCTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGgag                                                                                                                 <  3:327282/149‑1 (MQ=255)
                            cTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGgaga                                                                                                                <  1:838537/149‑1 (MQ=255)
                              ggCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATg                                                                                                              <  4:669787/149‑1 (MQ=255)
                               gCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGa                                                                                                             >  4:720416/1‑149 (MQ=255)
                                 gTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGaaa                                                                                                           <  1:853193/149‑1 (MQ=255)
                                 gTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGaaa                                                                                                           <  2:730314/149‑1 (MQ=255)
                                     aCCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTca                                                                                                       <  4:376126/149‑2 (MQ=255)
                                     aCCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCg                                                                                                       <  1:249380/149‑1 (MQ=255)
                                     aCCGCTGTTCGGTGACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGACGCCAGCGCCCTGGCGGTGAACGCTGCGCGTCGCATTAGCCCGGAGATGAAAGTCg                                                                                                       <  2:636525/149‑1 (MQ=255)
                                            ttCGGTTCCTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAACCCCGGAAGAGACGCTGTATCAGGTGCTGCATCACCAGGTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTAt                                                                                                >  1:828610/1‑149 (MQ=255)
                                            ttCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGaaa                                                                                                           <  4:8729/138‑1 (MQ=255)
                                            ttCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGaaa                                                                                                           >  3:8729/1‑138 (MQ=255)
                                                     tgctgcTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGaaa                                                                                                                                         >  1:64304/1‑99 (MQ=255)
                                                     tgctgcTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGaaa                                                                                                                                         <  2:64304/99‑1 (MQ=255)
                                                     tgctgcTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGAt                                                                                       >  4:356523/1‑149 (MQ=255)
                                                      gctgctCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATg                                                                                      >  1:716218/1‑149 (MQ=255)
                                                        tgctCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGt                                                                                    <  2:22130/149‑1 (MQ=255)
                                                         gctCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTg                                                                                   <  3:247715/149‑1 (MQ=255)
                                                         gctCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTg                                                                                   <  1:60368/149‑1 (MQ=255)
                                                           tCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGcc                                                                                 >  1:34218/1‑149 (MQ=255)
                                                             cGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGc                                                                               <  4:601982/149‑1 (MQ=255)
                                                                   ggtgTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATc                                                                         <  4:221914/149‑1 (MQ=255)
                                                                    gtgtATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATcc                                                                        <  2:547312/149‑1 (MQ=255)
                                                                      gtATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCtt                                                                      >  4:7222/1‑149 (MQ=255)
                                                                        ataCCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTAc                                                                    >  1:367381/1‑149 (MQ=255)
                                                                            cGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCt                                                                <  3:309535/149‑1 (MQ=255)
                                                                              aGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGt                                                              <  2:874280/149‑1 (MQ=255)
                                                                                 aTGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGga                                                                                                                  >  3:37456/1‑94 (MQ=255)
                                                                                 aTGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGga                                                                                                                  <  4:37456/94‑1 (MQ=255)
                                                                                 aTGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAAc                                                           >  4:12166/1‑149 (MQ=255)
                                                                                 aTGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAAc                                                           >  2:375993/1‑149 (MQ=255)
                                                                                 aTGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAAc                                                           >  1:582874/1‑149 (MQ=255)
                                                                                  tGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAAcc                                                          >  4:420135/1‑149 (MQ=255)
                                                                                      aaCCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGa                                                      <  1:193987/149‑1 (MQ=255)
                                                                                      aaCCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGa                                                      <  1:209074/149‑1 (MQ=255)
                                                                                         ccGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACga                                                   >  2:819175/1‑149 (MQ=255)
                                                                                          cGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACgat                                                  <  2:771449/149‑1 (MQ=255)
                                                                                             aaGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACgatgtg                                               <  3:171082/149‑1 (MQ=255)
                                                                                              agagACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTGTATCCTTACTCCTGTAACCCGGACgatgtga                                              <  1:52954/149‑1 (MQ=255)
                                                                                              agagACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACgatgtga                                              <  2:562987/149‑1 (MQ=255)
                                                                                                  aCGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGtt                                          >  3:179063/1‑149 (MQ=255)
                                                                                                    gATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCg                                        >  1:556600/1‑149 (MQ=255)
                                                                                                     aTGTATCAGGTGCTGCATCACCAGTTTGTCGACAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGCGATGAAAGACCGCGGGATGCTGGCGATGGTGCCGCGCTATCCCTACTCCTGTAACCCGCACGATGTGATGTTCGc                                       <  2:828610/149‑1 (MQ=255)
                                                                                                        tATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCa                                    <  2:512104/149‑1 (MQ=255)
                                                                                                         aTCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAg                                   <  2:34218/149‑1 (MQ=255)
                                                                                                         aTCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAg                                   <  2:430010/149‑1 (MQ=255)
                                                                                                          tCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCa                                    <  2:681341/147‑1 (MQ=255)
                                                                                                          tCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCa                                    >  1:681341/1‑147 (MQ=255)
                                                                                                          tCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAgg                                  >  3:298033/1‑149 (MQ=255)
                                                                                                          tCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAgg                                  >  2:411216/1‑149 (MQ=255)
                                                                                                           cAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACgatgtga                                              >  1:690226/1‑136 (MQ=255)
                                                                                                           cAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACgatgtga                                              <  2:690226/136‑1 (MQ=255)
                                                                                                           cAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGa                                 <  2:556600/149‑1 (MQ=255)
                                                                                                           cAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGa                                 <  4:392809/149‑1 (MQ=255)
                                                                                                             ggTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGt                               <  3:527452/149‑1 (MQ=255)
                                                                                                               tgctgcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCg                             <  2:319525/149‑1 (MQ=255)
                                                                                                                gctgcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGa                            >  3:806127/1‑149 (MQ=255)
                                                                                                                gctgcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGa                            <  2:649518/149‑1 (MQ=255)
                                                                                                                   gcATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATgc                         >  1:633890/1‑149 (MQ=255)
                                                                                                                      tCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGcgcg                      >  4:746037/1‑149 (MQ=255)
                                                                                                                       cACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAATCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGCACGATGTGATGTTCGCTCAGAAGTCGATGCGCGa                     >  2:298676/1‑149 (MQ=255)
                                                                                                                         ccAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGa                                                      <  3:279934/114‑1 (MQ=255)
                                                                                                                         ccAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGa                                                      >  4:279934/1‑114 (MQ=255)
                                                                                                                         ccAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAAc                   >  3:673978/1‑149 (MQ=255)
                                                                                                                          cAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACg                  >  3:134803/1‑149 (MQ=255)
                                                                                                                           ctttcaccGCCAGGGCGCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGc                 >  1:184277/8‑149 (MQ=255)
                                                                                                                             tttGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTa               >  2:159387/1‑149 (MQ=255)
                                                                                                                              ttGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTAc              <  3:168876/149‑1 (MQ=255)
                                                                                                                               caccGCCAGGGCGCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACg             >  4:449017/4‑149 (MQ=255)
                                                                                                                                gTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGt            >  2:41974/1‑149 (MQ=255)
                                                                                                                                 tCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTc           <  2:582874/149‑1 (MQ=255)
                                                                                                                                 ccGCCAGGGCGCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTAc              >  4:125542/2‑146 (MQ=255)
                                                                                                                                 ccGCCAGGGCGCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTAc              <  3:125542/145‑1 (MQ=255)
                                                                                                                                   gCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCtt         <  1:799670/149‑1 (MQ=255)
                                                                                                                                          cccTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGAt  <  2:10425/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CCTTCAACGAAATTAACAACCAGCGTAACTGGCGTGCACCGCTGTTCGGTTACTGCTGCTCCGGCGTGGTGTATACCGAGCATGAAAACCCGGAAGAGACGATGTATCAGGTGCTGCATCACCAGTTTGTCGCCAGCGCCCTGGCGGTGAAAGCTGCGCGTCGCATTAACCCGGAGATGAAAGTCGGCTGTATGCTGGCGATGGTGCCGCTCTATCCTTACTCCTGTAACCCGGACGATGTGATGTTCGCTCAGGAGTCGATGCGCGAACGCTACGTCTTTACCGAT  >  NC_000913/3044191‑3044477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: