Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,145 G→C 5.4% intergenic (+157/+33) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1450GC 5.4% 393.3 / 10.7 110intergenic (+157/+33)sibC/serAsRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (48/56);  new base C (2/4);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-01

Only 100 of 111 total aligned reads displayed.

GCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGG  >  NC_000913/3056998‑3057293
                                                                                                                                                   |                                                                                                                                                    
gCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGt                                                                                                                                                     <  1:733296/149‑1 (MQ=255)
 ccccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg                                                                                                                                                    >  4:120368/1‑149 (MQ=255)
   ccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAc                                                                                                                                                  <  2:612936/149‑1 (MQ=255)
     aTGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCt                                                                                                                                                <  4:775561/149‑1 (MQ=255)
       gctgcCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGc                                                                                                                                              <  2:330325/149‑1 (MQ=255)
       gctgcCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGc                                                                                                                                              >  2:301282/1‑149 (MQ=255)
         tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGccc                                                                                                                                            <  1:723076/149‑1 (MQ=255)
         tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                                                                            <  2:121588/149‑1 (MQ=255)
            cctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt                                                                                                                                         <  4:778690/149‑1 (MQ=255)
            cctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTCACCTGCCCGtt                                                                                                                                         <  3:692029/149‑1 (MQ=255)
              tgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttt                                                                                                                                       <  4:846592/149‑3 (MQ=255)
               gccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                                                                      <  4:511846/149‑1 (MQ=255)
                  aTCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtttt                                                                                                                                   >  1:175896/1‑149 (MQ=255)
                  aTCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtttt                                                                                                                                   <  2:852654/149‑1 (MQ=255)
                   tCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                                                                                  <  1:527740/149‑1 (MQ=255)
                        aaTCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAgaga                                                                                                                             <  4:3977/149‑1 (MQ=255)
                          tCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                                           >  2:775937/1‑149 (MQ=255)
                           ccccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                                                                          <  1:59433/149‑1 (MQ=255)
                           ccccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                                                                          >  2:514855/1‑149 (MQ=255)
                           ccccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                                                                          <  3:280952/149‑1 (MQ=255)
                            cccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAggg                                                                                                                         >  3:200104/1‑149 (MQ=255)
                             ccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                                        >  4:86751/1‑149 (MQ=255)
                              cATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                                                                       >  1:594241/1‑149 (MQ=255)
                              cATTAAATAAACACAACGCATTGATCTGACTTTGATTGATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                                                                       >  1:733787/1‑149 (MQ=255)
                               aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                                      <  1:806041/149‑1 (MQ=255)
                               aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                                      <  4:419584/149‑1 (MQ=255)
                               aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                                      >  2:402664/1‑149 (MQ=255)
                               aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                                      <  4:361913/149‑1 (MQ=255)
                               aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                                      >  4:645807/1‑149 (MQ=255)
                                ttAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAt                                                                                                                     >  3:843028/1‑149 (MQ=255)
                                ttAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAt                                                                                                                     >  3:16812/1‑149 (MQ=255)
                                  aaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                                                                   <  1:408625/149‑1 (MQ=255)
                                  aaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                                                                   <  4:162294/149‑1 (MQ=255)
                                  aaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                                                                   <  4:42790/149‑1 (MQ=255)
                                   aataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                                                                                  <  3:690664/149‑1 (MQ=255)
                                   aataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                                                                                  <  3:645807/149‑1 (MQ=255)
                                       aaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                                                                              <  3:120368/149‑1 (MQ=255)
                                        acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                                                                             <  3:417832/149‑1 (MQ=255)
                                        acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                                                                             <  3:79736/149‑1 (MQ=255)
                                          acaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                                                                           >  4:688575/1‑149 (MQ=255)
                                               gCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACgg                                                                                                      <  3:698866/149‑1 (MQ=255)
                                               gCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACgg                                                                                                      >  3:626839/1‑149 (MQ=255)
                                                 aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgc                                                                                                    <  2:566175/149‑1 (MQ=255)
                                                     aTCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGa                                                                                                >  2:11943/1‑149 (MQ=255)
                                                      tCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGaa                                                                                               <  3:345604/149‑1 (MQ=255)
                                                       cTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAt                                                                                              <  2:666989/149‑1 (MQ=255)
                                                        tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTGCAGCAGACGGGCGCGAATg                                                                                             <  1:677/149‑1 (MQ=255)
                                                        tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAta                                                                                             >  3:78504/1‑148 (MQ=255)
                                                        tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATg                                                                                             <  4:352050/149‑1 (MQ=255)
                                                        tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGAGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATg                                                                                             >  2:152778/1‑149 (MQ=255)
                                                            tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAGTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAc                                                                                         >  1:209108/1‑149 (MQ=255)
                                                                  ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGaa                                                                                   <  3:769840/149‑1 (MQ=255)
                                                                    atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATa                                                                                 <  4:799735/149‑1 (MQ=255)
                                                                    atttTCTGGAGCAGAATCTCAAAGTATAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACAGGCCAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGTGAAGTAGTACAGCATACGGGCGCGAATGGTACCCGGAATa                                                                                 <  4:760047/149‑1 (MQ=255)
                                                                     ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                                                                                >  4:665680/1‑149 (MQ=255)
                                                                     ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                                                                                >  3:611333/1‑149 (MQ=255)
                                                                       ttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCt                                                                              >  1:557533/1‑149 (MQ=255)
                                                                           ggAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                                                                          >  4:79631/1‑149 (MQ=255)
                                                                             aGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                                                                        <  4:744447/149‑1 (MQ=255)
                                                                                aGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                                                                     >  2:157133/1‑149 (MQ=255)
                                                                                 gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt                                                                    >  3:448806/1‑149 (MQ=255)
                                                                                    tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa                                                                 >  3:159096/1‑149 (MQ=255)
                                                                                      gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                                                               >  4:380287/1‑149 (MQ=255)
                                                                                      gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc                                                               >  4:346214/1‑149 (MQ=255)
                                                                                       cAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcg                                                              <  3:449355/149‑1 (MQ=255)
                                                                                         aaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCt                                                            <  2:668453/149‑1 (MQ=255)
                                                                                              gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAGAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTc                                                       <  2:594241/149‑1 (MQ=255)
                                                                                              gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTc                                                       <  4:71495/149‑1 (MQ=255)
                                                                                              gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTc                                                       >  3:371390/1‑149 (MQ=255)
                                                                                              gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTc                                                       >  3:57328/1‑149 (MQ=255)
                                                                                              gAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTc                                                       <  1:157133/149‑1 (MQ=255)
                                                                                               aaTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCg                                                      <  3:380287/149‑1 (MQ=255)
                                                                                                 tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGc                                                    >  2:99301/1‑149 (MQ=255)
                                                                                                    gcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAAc                                                 <  4:115534/149‑1 (MQ=255)
                                                                                                    gcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAAc                                                 >  3:813716/1‑149 (MQ=255)
                                                                                                    gcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAAc                                                 >  2:804807/1‑149 (MQ=255)
                                                                                                         gcgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCtt                                            <  4:448806/149‑1 (MQ=255)
                                                                                                          cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtc                                           <  4:88443/149‑1 (MQ=255)
                                                                                                               aCGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCgg                                      <  2:146567/149‑1 (MQ=255)
                                                                                                                  gtgtGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                                   >  1:840010/1‑149 (MQ=255)
                                                                                                                       gagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaata                              >  1:470536/1‑149 (MQ=255)
                                                                                                                         gaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatc                            >  2:226095/1‑149 (MQ=255)
                                                                                                                                tAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGaa                                                                                   <  4:645489/87‑1 (MQ=255)
                                                                                                                                tAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGaa                                                                                   >  3:645489/1‑87 (MQ=255)
                                                                                                                                 aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACca                    <  3:665680/149‑1 (MQ=255)
                                                                                                                                  aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACcac                   >  1:410625/1‑149 (MQ=255)
                                                                                                                                      aaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATaa               >  1:40241/1‑149 (MQ=255)
                                                                                                                                      aaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATaa               >  3:101601/1‑149 (MQ=255)
                                                                                                                                          ggCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa                                                                 >  3:90917/1‑95 (MQ=255)
                                                                                                                                          ggCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa                                                                 <  4:90917/95‑1 (MQ=255)
                                                                                                                                            cAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATc         >  1:188624/1‑149 (MQ=255)
                                                                                                                                             aaGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCt        <  2:209108/149‑1 (MQ=255)
                                                                                                                                             aaGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCt        >  1:620748/1‑149 (MQ=255)
                                                                                                                                              aGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAAc                                                 <  2:419545/107‑1 (MQ=255)
                                                                                                                                              aGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAAc                                                 >  1:419545/1‑107 (MQ=255)
                                                                                                                                               gTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTgg      >  1:478743/1‑149 (MQ=255)
                                                                                                                                                tCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTggg     >  4:639050/1‑149 (MQ=255)
                                                                                                                                                 cAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGGCGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGc    <  4:92826/149‑1 (MQ=255)
                                                                                                                                                 cAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGc    <  4:611333/149‑1 (MQ=255)
                                                                                                                                                   gTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCgg  >  2:388702/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGG  >  NC_000913/3056998‑3057293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: