Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 431,111 A→T 12.8% intergenic (+159/+18) yajD → / ← tsx HNH nuclease family protein/nucleoside channel, receptor of phage T6 and colicin K

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913431,1110AT12.8% 279.6 / 27.5 94intergenic (+159/+18)yajD/tsxHNH nuclease family protein/nucleoside channel, receptor of phage T6 and colicin K
Reads supporting (aligned to +/- strand):  ref base A (39/43);  new base T (5/7);  total (44/50)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

AATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAG  >  NC_000913/430964‑431259
                                                                                                                                                   |                                                                                                                                                    
aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAAt                                                                                                                                                     <  4:241664/149‑1 (MQ=255)
  ttGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATcc                                                                                                                                                   <  1:530803/149‑1 (MQ=255)
    gCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCg                                                                                                                                                 <  1:766005/149‑1 (MQ=255)
    gCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCg                                                                                                                                                 <  1:739200/149‑1 (MQ=255)
    gCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCg                                                                                                                                                 <  1:351525/149‑1 (MQ=255)
        gATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAt                                                                                                                                             <  4:881250/149‑1 (MQ=255)
        gATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAt                                                                                                                                             >  4:338720/1‑149 (MQ=255)
        gATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAt                                                                                                                                             <  4:109426/149‑1 (MQ=255)
        gATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCAt                                                                                                                                             <  2:842767/149‑1 (MQ=255)
           gcgcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtttc                                                                                                                                          <  4:870156/149‑2 (MQ=255)
            cgcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTc                                                                                                                                         >  4:739437/1‑149 (MQ=255)
             gcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAAt                                                                                                                                                     >  4:328641/1‑136 (MQ=255)
             gcTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAAt                                                                                                                                                     <  3:328641/136‑1 (MQ=255)
              cTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtttctga                                                                                                                                       <  2:699310/149‑5 (MQ=37)
                  gCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATc                                                                                                                                   >  1:236342/1‑149 (MQ=255)
                  gCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATc                                                                                                                                   <  1:738121/149‑1 (MQ=255)
                    ttATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAg                                                                                                                                 >  4:383104/1‑149 (MQ=255)
                      aTCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtttc                                                                                                                                          <  4:393936/138‑2 (MQ=255)
                      aTCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtttc                                                                                                                                          >  3:393936/1‑137 (MQ=255)
                      aTCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGaa                                                                                                                               <  3:135744/149‑1 (MQ=255)
                            ccTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTa                                                                                                                         <  2:236342/149‑1 (MQ=255)
                             cTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTaa                                                                                                                        <  3:636841/149‑1 (MQ=255)
                             cTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTaa                                                                                                                        >  1:532527/1‑149 (MQ=255)
                              tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAc                                                                                                                       >  4:143769/1‑149 (MQ=255)
                              tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAc                                                                                                                       >  1:7093/1‑149 (MQ=255)
                              tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAc                                                                                                                       >  1:607436/1‑149 (MQ=255)
                              tACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAAc                                                                                                                       >  3:238894/1‑149 (MQ=255)
                                        cAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAg                                                                                                             <  3:522257/149‑1 (MQ=255)
                                        cAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAg                                                                                                             <  1:587985/149‑1 (MQ=255)
                                         aGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAgg                                                                                                            >  4:823634/1‑149 (MQ=255)
                                           cAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTa                                                                                                          >  4:372936/1‑149 (MQ=255)
                                            aaTATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTaa                                                                                                         <  1:23189/149‑1 (MQ=255)
                                                  ttGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCAccc                                                                                                   >  1:544342/1‑149 (MQ=255)
                                                  ttGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCAccc                                                                                                   >  2:485651/1‑149 (MQ=255)
                                                  ttGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCAccc                                                                                                   >  1:188658/1‑149 (MQ=255)
                                                      aTTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGc                                                                                               >  1:339752/1‑149 (MQ=255)
                                                            gTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtttc                                                                                                                                          >  1:491579/1‑99 (MQ=37)
                                                            gTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGATTTATTCCCGGCAtttc                                                                                                                                          <  2:491579/100‑2 (MQ=25)
                                                            gTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTag                                                                                         >  1:683157/1‑149 (MQ=255)
                                                                ttttGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCg                                                                                     >  1:429059/1‑149 (MQ=255)
                                                                ttttGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCg                                                                                     >  3:249559/1‑149 (MQ=255)
                                                                   tgttgGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAAc                                                                                  <  1:510853/149‑1 (MQ=255)
                                                                      tgGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGtt                                                                               <  4:238894/149‑1 (MQ=255)
                                                                         ccGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCgg                                                                                            >  4:123557/1‑133 (MQ=255)
                                                                         ccGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCgg                                                                                            <  3:123557/133‑1 (MQ=255)
                                                                         ccGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGaa                                                                            <  1:485651/149‑1 (MQ=255)
                                                                         ccGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGaa                                                                            >  3:725173/1‑149 (MQ=255)
                                                                         ccGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGaa                                                                            <  2:532527/149‑1 (MQ=255)
                                                                              cAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGCTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCATCCGGTAGAGCGAACGTTGAAgttgc                                                                       >  4:254386/1‑149 (MQ=255)
                                                                               aaaGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgcc                                                                      >  3:354453/1‑149 (MQ=255)
                                                                                  gCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgtt                                                                   <  1:645627/149‑1 (MQ=255)
                                                                                    gTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTaa                                                                                                                        >  4:206201/1‑94 (MQ=255)
                                                                                    gTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTaa                                                                                                                        <  3:206201/94‑1 (MQ=255)
                                                                                    gTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgttgc                                                                 <  3:383104/149‑1 (MQ=255)
                                                                                     tttACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAgttgccgttgcc                                                                >  1:890914/1‑149 (MQ=255)
                                                                                       tACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGa                                                              >  3:756702/1‑149 (MQ=255)
                                                                                         cgccgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAag                                                            <  4:619843/149‑1 (MQ=255)
                                                                                           ccgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagtt                                                          <  4:779847/149‑1 (MQ=255)
                                                                                            cgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttc                                                         >  3:401680/1‑149 (MQ=255)
                                                                                               aTCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttcagt                                                      <  2:179808/149‑1 (MQ=255)
                                                                                                tCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttcagtt                                                     <  3:489045/149‑1 (MQ=255)
                                                                                                       tGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtc                                              <  2:722055/149‑1 (MQ=255)
                                                                                                            aaaGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATcgtcgt                                         <  4:531393/149‑1 (MQ=255)
                                                                                                            aaaGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATcgtcgt                                         >  4:534907/1‑149 (MQ=255)
                                                                                                            aaaGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATcgtcgt                                         <  3:340279/149‑1 (MQ=255)
                                                                                                              aGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTc                                       <  4:354453/149‑1 (MQ=255)
                                                                                                               gCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTcc                                      >  2:712693/1‑149 (MQ=255)
                                                                                                               gCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTcc                                      <  4:249559/149‑1 (MQ=255)
                                                                                                               gCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTcc                                      <  2:429059/149‑1 (MQ=255)
                                                                                                                 acacGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACTTACTACCAGGTAACGACCCCAGCCGGTAGAGCGAACGTTAAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAGCGCCGTTCCAc                                    >  4:143589/1‑149 (MQ=255)
                                                                                                                 acacGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCAc                                    <  1:661410/149‑1 (MQ=255)
                                                                                                                 acacGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCAc                                    >  4:424194/1‑149 (MQ=255)
                                                                                                                 acacGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCAc                                    >  1:313147/1‑149 (MQ=255)
                                                                                                                    cGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGa                                 <  2:601704/149‑1 (MQ=255)
                                                                                                                     gttgttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttca                                                        >  1:84092/1‑125 (MQ=255)
                                                                                                                     gttgttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttca                                                        <  2:84092/125‑1 (MQ=255)
                                                                                                                        gttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAagttcagt                                                      <  3:290382/124‑1 (MQ=255)
                                                                                                                        gtgaaCAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTAGCCGAagttcagt                                                      >  4:290382/4‑124 (MQ=255)
                                                                                                                         ttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGaccacc                            >  1:435187/1‑149 (MQ=255)
                                                                                                                              aaTCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTCGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACcgtcgt                       >  4:774390/1‑149 (MQ=255)
                                                                                                                               aTCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTg                      >  1:296873/1‑149 (MQ=255)
                                                                                                                                tCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGc                     <  3:803960/149‑1 (MQ=255)
                                                                                                                                    aaaTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGtt                                                                               <  2:390067/87‑1 (MQ=38)
                                                                                                                                    aaaTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGtt                                                                               >  1:390067/1‑87 (MQ=38)
                                                                                                                                    aaaTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGt                 >  3:797029/1‑149 (MQ=255)
                                                                                                                                      aTGCCGGGATTTATTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa               >  2:350457/1‑149 (MQ=255)
                                                                                                                                      aTGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa               <  2:237488/149‑1 (MQ=255)
                                                                                                                                      aTGCCAGGAAAAAATCCCGGCAATTGCATAATCAGAAGTTGTAGCGTACTACCAGGTAACCACCCCAGCCGGTAGAGTGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTCCCACTGACCACCGTCGTGCCAGTaa               <  2:607436/149‑1 (MQ=255)
                                                                                                                                       tGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAAc              <  2:7093/149‑1 (MQ=255)
                                                                                                                                       tGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAAc              >  3:205334/1‑149 (MQ=255)
                                                                                                                                         ccGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGac                                <  1:652619/129‑1 (MQ=255)
                                                                                                                                         ccGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGac                                >  2:652619/1‑129 (MQ=255)
                                                                                                                                              aaTAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTAc       <  1:158745/149‑1 (MQ=255)
                                                                                                                                              aaTAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTAc       <  3:338720/149‑1 (MQ=255)
                                                                                                                                                 aaaTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGAc    >  3:73570/1‑149 (MQ=255)
                                                                                                                                                   aTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACag  >  4:189993/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTATTTCAGCAATATATTGAATTTACGTGCTTTTGTTGGCCGGACAAAGCGTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAG  >  NC_000913/430964‑431259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: