Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,111,961 T→C 5.4% pseudogene (130/963 nt) yghE ← pseudogene, secretion pathway protein, L‑type protein homology; putative general secretion pathway for protein export (GSP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,111,9610TC 5.4% 341.3 / 5.3 107pseudogene (130/963 nt)yghEpseudogene, secretion pathway protein, L‑type protein homology; putative general secretion pathway for protein export (GSP)
Reads supporting (aligned to +/- strand):  ref base T (51/50);  new base C (3/3);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 108 total aligned reads displayed.

GCCAGCCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAA  >  NC_000913/3111816‑3112109
                                                                                                                                                 |                                                                                                                                                    
gccagcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGtt                                                                                                                                                   <  3:756227/149‑1 (MQ=255)
 ccagcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGTACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTg                                                                                                                                                  <  2:847517/149‑1 (MQ=255)
     cccGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCa                                                                                                                                              <  3:623077/149‑1 (MQ=255)
        ggCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCatat                                                                                                                                           <  1:286421/149‑1 (MQ=255)
        ggCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAACATTGCGCagtg                                                                                                                                           <  1:406582/149‑4 (MQ=255)
          cgcTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGc                                                                                                                                         <  1:82347/149‑1 (MQ=255)
          cgcTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGc                                                                                                                                         <  4:571942/149‑1 (MQ=255)
          cgcTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAACATTGCGCagtggt                                                                                                                                         <  1:19549/149‑6 (MQ=255)
             tCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGt                                                                                                                                      >  3:181910/1‑149 (MQ=255)
              ccATCCCCGGCATTCATCGATGCGGCAGCTAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGtt                                                                                                                                     >  3:208969/1‑149 (MQ=255)
               cATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGTATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGCTTTCCTGCAACCACTGCGCAACGTTGCGCATATGCTGTTg                                                                                                                                    <  1:259532/149‑1 (MQ=255)
               cATCCCCGGCATCCATCTATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTTCGGCGCGACGGTAGCGCGGGTGATGACGTTTTATTGCAACCACTGCGCAATGTTGCGCATATGCTGTTg                                                                                                                                    >  4:346536/1‑149 (MQ=255)
                 tCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAGGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGt                                                                                                                                  <  4:735388/149‑1 (MQ=255)
                   cccGGCATTCATTGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGa                                                                                                                                <  1:515576/149‑1 (MQ=255)
                   cccGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGa                                                                                                                                >  1:46462/1‑149 (MQ=255)
                   cccGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGa                                                                                                                                >  4:270059/1‑149 (MQ=255)
                         aTTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACt                                                                                                                          >  2:356791/1‑149 (MQ=255)
                          ttCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTg                                                                                                                         >  2:378929/1‑149 (MQ=255)
                                  tGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGtt                                                                                                                 <  2:826293/149‑1 (MQ=255)
                                  tGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGtt                                                                                                                 <  2:791016/149‑1 (MQ=255)
                                  tGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGtt                                                                                                                 <  2:705027/149‑1 (MQ=255)
                                              cccGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTgg                                                                                                     >  3:722448/1‑149 (MQ=255)
                                               ccGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGt                                                                                                    <  4:235518/149‑1 (MQ=255)
                                                 gTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCg                                                                                                  >  4:418849/1‑149 (MQ=255)
                                                  ttGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGa                                                                                                 >  1:179033/1‑149 (MQ=255)
                                                   tGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGat                                                                                                <  4:616979/149‑1 (MQ=255)
                                                    gcgcATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGata                                                                                               >  3:598616/1‑149 (MQ=255)
                                                      gcATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGaa                                                                                                                               >  3:55876/1‑115 (MQ=255)
                                                      gcATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGaa                                                                                                                               <  4:55876/115‑1 (MQ=255)
                                                         tCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGtt                                                                                                                                     >  2:477305/1‑106 (MQ=255)
                                                         tCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGtt                                                                                                                                     <  1:477305/106‑1 (MQ=255)
                                                         tCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCaa                                                                                          >  1:892460/1‑149 (MQ=255)
                                                         tCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCaa                                                                                          >  1:276026/1‑149 (MQ=255)
                                                          cGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAAc                                                                                         <  4:747230/149‑1 (MQ=255)
                                                           gCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACg                                                                                        <  3:708809/149‑1 (MQ=255)
                                                           gCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACg                                                                                        >  3:42721/1‑149 (MQ=255)
                                                             aaGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACggg                                                                                      <  2:297220/149‑1 (MQ=255)
                                                                ccATAAACCCACAAGGAATGGACATCCAGTCAGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCg                                                                                   >  2:491675/1‑149 (MQ=255)
                                                                 cATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCat                                                                                                                                             >  3:489240/1‑90 (MQ=255)
                                                                 cATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCat                                                                                                                                             <  4:489240/90‑1 (MQ=255)
                                                                 cATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGa                                                                                  >  3:327806/1‑149 (MQ=255)
                                                                  aTAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGtt                                                                                                          <  2:354197/124‑1 (MQ=255)
                                                                  aTAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGtt                                                                                                          >  1:354197/1‑124 (MQ=255)
                                                                  aTAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGTGaa                                                                                 >  4:76011/1‑149 (MQ=255)
                                                                  aTAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGaa                                                                                 >  1:654028/1‑149 (MQ=255)
                                                                  aTAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGaa                                                                                 >  3:473347/1‑149 (MQ=255)
                                                                    aaaCCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAAt                                                                               >  3:336088/1‑149 (MQ=255)
                                                                     aaCCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATg                                                                              >  3:13641/1‑149 (MQ=255)
                                                                     aaCCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATg                                                                              >  4:536053/1‑149 (MQ=255)
                                                                       cccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCa                                                                            <  2:282887/149‑1 (MQ=255)
                                                                       cccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCa                                                                            <  1:836405/149‑1 (MQ=255)
                                                                        ccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAg                                                                           <  1:356791/149‑1 (MQ=255)
                                                                        ccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAg                                                                           <  4:408387/149‑1 (MQ=255)
                                                                          acaAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGtt                                                                         >  1:874354/1‑149 (MQ=255)
                                                                          acaAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGtt                                                                         >  1:71700/1‑149 (MQ=255)
                                                                           caAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTc                                                                        <  2:892460/149‑1 (MQ=255)
                                                                           caAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTc                                                                        >  3:163305/1‑149 (MQ=255)
                                                                            aaGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCa                                                                       >  2:658314/1‑149 (MQ=255)
                                                                            aaGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCa                                                                       <  4:722448/149‑1 (MQ=255)
                                                                            aaGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACAACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCa                                                                       >  2:283489/1‑149 (MQ=255)
                                                                              ggAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCACTGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATc                                                                     <  3:153847/149‑1 (MQ=255)
                                                                              ggAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATc                                                                     <  1:896593/149‑1 (MQ=255)
                                                                                   ggACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACgg                                                                                       <  3:866473/126‑1 (MQ=255)
                                                                                    gACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATc                                                               >  1:343493/1‑149 (MQ=255)
                                                                                    gACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACAACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATc                                                               >  2:767725/1‑149 (MQ=255)
                                                                                       aTCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTg                                                            <  2:250973/149‑1 (MQ=255)
                                                                                       aTCCAGTCCGGCACGACGGTAGCGCGGGTGATGCCGTTTTCCATCAACCACTGCGCAATGTTGAGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGGTGCTCGTCGATATTCAACGGGGCGAAATGCAGTTCAGCGATATTCTg                                                            >  4:219609/1‑149 (MQ=255)
                                                                                        tCCAGTCCGGCGCGACGGTATCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCACAAATGCTGTTGGTGAATCACTACTACGGTAGCCAGTTACTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTgg                                                           <  2:850744/149‑1 (MQ=255)
                                                                                        tCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTgg                                                           <  3:536053/149‑1 (MQ=255)
                                                                                         ccAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCa                                                                            >  2:255579/1‑131 (MQ=255)
                                                                                         ccAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCa                                                                            <  1:255579/131‑1 (MQ=255)
                                                                                          cAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGc                                                                                                               <  3:619334/95‑1 (MQ=255)
                                                                                          cAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGc                                                                                                               >  4:619334/1‑95 (MQ=255)
                                                                                          cAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGtt                                                         >  2:96313/1‑149 (MQ=255)
                                                                                            gtcggacgcgACGGTAGCGCGGGTGAAGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCTTATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCa                                                       >  4:560793/7‑149 (MQ=255)
                                                                                            gTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCa                                                       >  2:718509/1‑149 (MQ=255)
                                                                                                ggCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGctc                                                   <  4:181910/149‑1 (MQ=255)
                                                                                                 gcgcGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGctct                                                  <  2:471172/149‑1 (MQ=255)
                                                                                                            gcgcGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTaa                                       >  2:472850/1‑149 (MQ=255)
                                                                                                            gcgcGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTaa                                       >  2:720900/1‑149 (MQ=255)
                                                                                                               cgGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGc                                    <  3:794095/149‑1 (MQ=255)
                                                                                                               cgGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGc                                    <  2:276026/149‑1 (MQ=255)
                                                                                                                gggTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCg                                   >  3:257683/1‑149 (MQ=255)
                                                                                                                 ggTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCgg                                  <  2:654028/149‑1 (MQ=255)
                                                                                                                  gTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCggg                                 <  4:780060/149‑1 (MQ=255)
                                                                                                                         cGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATa                          <  4:386792/149‑1 (MQ=255)
                                                                                                                         cGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATa                          <  1:378929/149‑1 (MQ=255)
                                                                                                                            tttCCTGCGACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTc                       <  2:457512/149‑1 (MQ=255)
                                                                                                                            tttCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTc                       <  4:11530/149‑1 (MQ=255)
                                                                                                                              tCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCgg                     <  4:473347/149‑1 (MQ=255)
                                                                                                                              tCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCgg                     <  4:790906/149‑1 (MQ=255)
                                                                                                                                 tGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCACCGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTag                  >  1:839762/1‑149 (MQ=255)
                                                                                                                                 tGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTag                  >  1:160032/1‑149 (MQ=255)
                                                                                                                                   cAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcg                >  1:5008/1‑149 (MQ=255)
                                                                                                                                       cACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcagc            >  2:731068/1‑149 (MQ=255)
                                                                                                                                       cACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcact            >  2:443197/1‑147 (MQ=255)
                                                                                                                                       atatGCGCAACATTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcagc            >  1:27625/4‑149 (MQ=255)
                                                                                                                                        tatGCGCAACATTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcagcg           >  3:65654/3‑149 (MQ=255)
                                                                                                                                        tatGCGCAACATTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcagcg           >  2:243355/3‑149 (MQ=255)
                                                                                                                                        aCTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAACTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcagcg           >  2:451907/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCCAGCCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAA  >  NC_000913/3111816‑3112109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: