Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,164,290 A→T 6.0% S562T (TCG→ACG)  parC ← DNA topoisomerase IV, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,164,2900AT 6.0% 287.0 / 7.6 83S562T (TCG→ACG) parCDNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base A (38/40);  new base T (2/3);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

AGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGCCTTTA  >  NC_000913/3164142‑3164435
                                                                                                                                                     |                                                                                                                                                 
aGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGa                                                                                                                                                   >  2:505980/1‑149 (MQ=255)
     gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                              >  4:590204/1‑149 (MQ=255)
     gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                              >  1:291743/1‑149 (MQ=255)
     gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                              <  1:838356/149‑1 (MQ=255)
     gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                              >  4:119932/1‑149 (MQ=255)
     gTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCa                                                                                                                                              <  1:516680/149‑1 (MQ=255)
      ttAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAg                                                                                                                                             >  2:155868/1‑149 (MQ=255)
       tAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGc                                                                                                                                            <  3:164419/149‑1 (MQ=255)
          aGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtg                                                                                                                                          <  4:694235/149‑6 (MQ=255)
               cAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCgg                                                                                                                                    <  1:102959/149‑1 (MQ=255)
                aGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCggg                                                                                                                                   <  1:222986/149‑1 (MQ=255)
                    gAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCaa                                                                                                                               <  2:395184/149‑1 (MQ=255)
                     aaaCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAAt                                                                                                                              <  1:271560/149‑1 (MQ=255)
                     aaaCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCAACGGCAGCGTAATCGGGACAAt                                                                                                                              <  4:587365/149‑1 (MQ=255)
                      aaCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATg                                                                                                                             <  4:854515/149‑1 (MQ=255)
                       aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtgg                                                                                                                                         <  3:106008/137‑7 (MQ=255)
                       aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtgg                                                                                                                                         >  4:106008/1‑131 (MQ=255)
                       aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATgg                                                                                                                            <  2:388734/149‑1 (MQ=255)
                       aCCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATgg                                                                                                                            >  3:847283/1‑149 (MQ=255)
                        ccGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGc                                                                                                                           >  2:224920/1‑149 (MQ=255)
                             aCCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGc                                                                                                                      <  1:226977/149‑1 (MQ=255)
                               ccGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtg                                                                                                                                          <  4:364931/128‑6 (MQ=255)
                               ccGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGCTGCCGTCGGcgcgtg                                                                                                                                          >  3:364931/1‑123 (MQ=255)
                                cGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTAc                                                                                                                   <  2:875956/149‑1 (MQ=255)
                                cGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTAc                                                                                                                   <  3:317907/149‑1 (MQ=255)
                                      ggAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGCCCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCgg                                                                                                             >  4:109919/1‑149 (MQ=255)
                                             aTCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATc                                                                                                      <  2:883451/149‑1 (MQ=255)
                                             aTCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATc                                                                                                      >  4:147425/1‑149 (MQ=255)
                                              tCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCATCGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATca                                                                                                     >  3:576574/1‑149 (MQ=255)
                                               cagcagTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATcaa                                                                                                    <  2:533984/149‑1 (MQ=255)
                                               cagcagTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAACcaa                                                                                                    <  2:143538/149‑1 (MQ=255)
                                                   agTTTCTGATCGTCGCTTTCCAACAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCaacaaa                                                                                                <  2:789233/149‑1 (MQ=255)
                                                     tttCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCaacaaaca                                                                                              >  1:475944/1‑149 (MQ=255)
                                                      ttCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAacac                                                                                             <  2:718164/149‑1 (MQ=255)
                                                       tCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACt                                                                                            >  1:95319/1‑149 (MQ=255)
                                                         tGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTAc                                                                                          >  3:358010/1‑149 (MQ=255)
                                                           aTCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCg                                                                                        >  4:482097/1‑149 (MQ=255)
                                                           aTCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCg                                                                                        <  3:727715/149‑1 (MQ=255)
                                                             cgtcgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCgg                                                                                                                                    <  2:881321/103‑1 (MQ=255)
                                                             cgtcgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCgg                                                                                                                                    >  1:881321/1‑103 (MQ=255)
                                                               tcgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCggttg                                                                                    >  3:195454/1‑149 (MQ=255)
                                                                cgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCggttgg                                                                                   <  2:95319/149‑1 (MQ=255)
                                                                cgCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCggttgg                                                                                   <  3:245375/149‑1 (MQ=255)
                                                                   tttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGgttggttg                                                                                >  4:371842/1‑149 (MQ=255)
                                                                   tttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGgttggttg                                                                                >  4:263850/1‑149 (MQ=255)
                                                                   tttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTCAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGgttggttg                                                                                >  4:625523/1‑149 (MQ=255)
                                                                    ttCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGc                                                                               >  3:274738/1‑149 (MQ=255)
                                                                     tCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGct                                                                              >  2:756068/1‑149 (MQ=255)
                                                                      ccATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGctc                                                                             <  3:521724/149‑1 (MQ=255)
                                                                        aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGTCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                           >  3:508501/1‑149 (MQ=255)
                                                                        aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                           >  2:727432/1‑149 (MQ=255)
                                                                        aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                           >  1:766949/1‑149 (MQ=255)
                                                                        aTCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCtt                                                                           >  1:67889/1‑149 (MQ=255)
                                                                          cAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTAc                                                                         >  1:763973/1‑149 (MQ=255)
                                                                          cAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTCAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTAc                                                                         <  3:625523/149‑1 (MQ=255)
                                                                             cATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATGTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCtt                                                                      <  4:546426/149‑1 (MQ=255)
                                                                               tatGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTc                                                                    >  3:719082/1‑149 (MQ=255)
                                                                                 tGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAc                                                                  <  3:371842/149‑1 (MQ=255)
                                                                                  ggTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCAcc                                                                 >  2:670916/1‑149 (MQ=255)
                                                                                          gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCttt                                                         <  3:162972/149‑1 (MQ=255)
                                                                                          gTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCttt                                                         <  4:274738/149‑1 (MQ=255)
                                                                                             gCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGaa                                                      <  3:590204/149‑1 (MQ=255)
                                                                                             gCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGaa                                                      <  4:261475/149‑1 (MQ=255)
                                                                                               cccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGc                                                    >  2:363200/1‑149 (MQ=255)
                                                                                                ccAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCt                                                   >  2:791477/1‑149 (MQ=255)
                                                                                                 cAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTa                                                  <  3:665281/149‑1 (MQ=255)
                                                                                                   ggcggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATc                                                >  2:288917/1‑149 (MQ=255)
                                                                                                     cggcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAc                                              >  4:751927/1‑149 (MQ=255)
                                                                                                       gcAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCAccc                                            <  1:852328/149‑1 (MQ=255)
                                                                                                         aaCGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCAGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGc                                          >  2:732548/1‑149 (MQ=255)
                                                                                                                aTTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATaa                                   <  3:8001/149‑1 (MQ=255)
                                                                                                                 tttGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAt                                  >  2:727241/1‑149 (MQ=255)
                                                                                                                  ttGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAAtt                                 <  1:505980/149‑1 (MQ=255)
                                                                                                                    gCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGTTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCa                               <  4:81537/149‑1 (MQ=255)
                                                                                                                               gCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGcgcg                    >  4:262975/1‑149 (MQ=255)
                                                                                                                                cTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGt                   >  1:375215/1‑149 (MQ=255)
                                                                                                                                     ccTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTAc                                                                                          <  1:179394/73‑1 (MQ=255)
                                                                                                                                     ccTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTAc                                                                                          >  2:179394/1‑73 (MQ=255)
                                                                                                                                     ccTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGata              >  4:346097/1‑149 (MQ=255)
                                                                                                                                         aCCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCAt          >  2:549345/1‑149 (MQ=255)
                                                                                                                                          ccACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATg         <  3:119932/149‑1 (MQ=255)
                                                                                                                                            aCGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGc       <  3:482097/149‑1 (MQ=255)
                                                                                                                                             cgc‑gCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAACCACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGcc      <  3:819016/149‑1 (MQ=255)
                                                                                                                                                  ccGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGCCTTTa  <  1:224920/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AGATCGTTAAAGGTGCAGACGAAACCGTAACCCGCATCGGAAGCCATCAGCAGTTTCTGATCGTCGCTTTCCATCAGCATATGGTCAACGGTCGCCCCAGGCGGCAACGTTAATTTGCCGGTGAGCGGCTCGCCCTGACCACGC‑GCCGACGGCAGCGTAATCGGGTCAATGGCATAGCTACGACCGGTGGAATCAACAAACACTACCGGTTGGTTGCTCTTACCTTTCACCGCCGCTTTGAAGCTATCACCCGCTTTATAATTCAGGCCCGGCGCGTCGATATCATGGCCTTTA  >  NC_000913/3164142‑3164435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑