Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,959 C→T 9.3% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9590CT 9.3% 279.4 / 13.9 96intergenic (‑48/+42)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base C (42/45);  new base T (3/6);  total (45/51)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

GTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCC  >  NC_000913/3324814‑3325096
                                                                                                                                                 |                                                                                                                                         
gTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCg                                                                                                                                        <  2:655725/149‑1 (MQ=255)
 tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACTTGAAGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATTAATTGATAAGAAAAAACCCCGGAGCACGCCCCgg                                                                                                                                       <  4:648945/149‑1 (MQ=255)
    caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGt                                                                                                                                    <  1:751376/149‑1 (MQ=255)
     accaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtt                                                                                                                                   <  1:183797/149‑1 (MQ=255)
      tcaTCCGAAAACGAACCGGACGTGACGATGAGGATCCCAATTTCGTGAGGATGGCTAAGGAAAAGTGAAGTACCCTGGCCACAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttt                                                                                                                                  <  1:339555/148‑1 (MQ=255)
       caTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGGTGTCATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtttt                                                                                                                                 <  2:817537/149‑1 (MQ=255)
        aTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                >  2:103057/1‑149 (MQ=255)
         tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGtttttt                                                                                                                               <  4:381716/149‑3 (MQ=255)
         tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttat                                                                                                                               <  4:813442/149‑3 (MQ=255)
         tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCg                                                                                                                               >  1:344853/1‑149 (MQ=255)
         tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCg                                                                                                                               <  1:380564/149‑1 (MQ=255)
         tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCg                                                                                                                               <  2:860804/149‑1 (MQ=255)
         tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGCGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCg                                                                                                                               <  2:850461/149‑1 (MQ=255)
          ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttc                                                                                                                              <  3:305256/149‑4 (MQ=255)
          ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttc                                                                                                                              <  3:118005/149‑4 (MQ=255)
            gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTa                                                                                                                            >  2:589323/1‑149 (MQ=255)
            gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTa                                                                                                                            >  1:745732/1‑149 (MQ=255)
              aaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCCGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAcc                                                                                                                          <  2:320852/149‑2 (MQ=255)
                    aTCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACa                                                                                                                    <  3:67661/149‑1 (MQ=255)
                     tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                   >  1:478362/1‑149 (MQ=255)
                     tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                   >  3:676223/1‑149 (MQ=255)
                       aGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTc                                                                                                                 <  3:469432/149‑1 (MQ=255)
                          cGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                              <  3:338117/149‑1 (MQ=255)
                          cGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                              <  3:682677/149‑1 (MQ=255)
                           gTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATACGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCt                                                                                                             <  2:767295/149‑1 (MQ=255)
                                cttGAGGATCCCCATTACAAGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGCTTCCTGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGc                                                                                                        <  2:640797/148‑1 (MQ=255)
                                 ttGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCg                                                                                                       <  2:889944/149‑1 (MQ=255)
                                     ggATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGaa                                                                                                   <  1:54290/149‑1 (MQ=255)
                                      gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                  <  1:103057/149‑1 (MQ=255)
                                      gATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                  <  1:845908/149‑1 (MQ=255)
                                          cccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttac                                                                                              <  1:834417/149‑1 (MQ=255)
                                          cccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttac                                                                                              <  2:559282/149‑1 (MQ=255)
                                           ccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAGAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGaa                                                                                                   >  3:858725/1‑143 (MQ=255)
                                           ccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAGAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGaa                                                                                                   <  4:858725/143‑1 (MQ=255)
                                           ccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttact                                                                                             >  2:471220/1‑149 (MQ=255)
                                           ccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGNANNAANACAGTCATCTGATGCGGGAacttact                                                                                             >  3:688929/1‑149 (MQ=255)
                                            cATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                            >  4:235227/1‑149 (MQ=255)
                                             aTTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCGTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTg                                                                                           >  2:626740/1‑149 (MQ=255)
                                               tACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTc                                                                                         <  3:817564/149‑1 (MQ=255)
                                                  gTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGcc                                                                                      >  2:46824/1‑149 (MQ=255)
                                                     aGGATGGCTAAGGGCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTaa                                                                                   <  2:550321/149‑1 (MQ=255)
                                                      ggATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAAc                                                                                  >  3:95021/1‑149 (MQ=255)
                                                          ggCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGct                                                                              >  3:326650/1‑149 (MQ=255)
                                                             tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                           >  1:257790/1‑149 (MQ=255)
                                                             tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                           >  1:350461/1‑149 (MQ=255)
                                                             tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                           >  1:690517/1‑149 (MQ=255)
                                                                ggTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACa                                                                        <  1:115482/149‑1 (MQ=255)
                                                                    cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                    <  3:159529/149‑1 (MQ=255)
                                                                        gAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTa                                                                <  1:471220/149‑1 (MQ=255)
                                                                              cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                          >  4:343589/1‑149 (MQ=255)
                                                                               ccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                      <  4:336018/136‑1 (MQ=255)
                                                                               ccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                      >  3:336018/1‑136 (MQ=255)
                                                                                 tGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTc                                                       <  4:749773/149‑1 (MQ=255)
                                                                                    gCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGc                                                    <  1:589323/149‑1 (MQ=255)
                                                                                            tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                      <  4:620587/123‑1 (MQ=255)
                                                                                            tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                      >  3:620587/1‑123 (MQ=255)
                                                                                            tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                            >  4:776172/1‑149 (MQ=255)
                                                                                            tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                            <  2:756116/149‑1 (MQ=255)
                                                                                             ttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                           <  2:677711/149‑1 (MQ=255)
                                                                                               cATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtc                                         >  3:831695/1‑149 (MQ=255)
                                                                                                 tgatgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcat                                       <  1:74791/149‑1 (MQ=255)
                                                                                                 tgatgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcat                                       >  2:277170/1‑149 (MQ=255)
                                                                                                     gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAc                                   <  2:257790/149‑1 (MQ=255)
                                                                                                     gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAc                                   <  3:485569/149‑1 (MQ=255)
                                                                                                        aTCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCgg                                >  1:8860/1‑149 (MQ=255)
                                                                                                           ccTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACg                             >  2:306604/1‑149 (MQ=255)
                                                                                                           ccTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACg                             >  2:484543/1‑149 (MQ=255)
                                                                                                            cTGGTATGAAGTGGTAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACga                            >  2:511874/1‑149 (MQ=255)
                                                                                                             tGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgag                           <  4:535665/149‑1 (MQ=255)
                                                                                                               gTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                            <  3:449785/130‑1 (MQ=255)
                                                                                                               gTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                            >  4:449785/1‑130 (MQ=255)
                                                                                                               gTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgagga                         <  3:235227/149‑1 (MQ=255)
                                                                                                                 aTGAATTGATATGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                            >  3:773941/1‑80 (MQ=255)
                                                                                                                 aTGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                            <  4:773941/80‑1 (MQ=255)
                                                                                                                 aTGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGc                       >  1:329317/1‑149 (MQ=255)
                                                                                                                  tGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGcc                      >  1:104342/1‑149 (MQ=255)
                                                                                                                     aTTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCttt                   >  4:654201/1‑149 (MQ=255)
                                                                                                                      ttGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTg                  <  3:654201/149‑1 (MQ=255)
                                                                                                                        gATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGa                <  4:688929/149‑1 (MQ=255)
                                                                                                                        gATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATAAAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGa                >  2:167992/1‑149 (MQ=255)
                                                                                                                         aTAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGAc               <  3:315837/149‑1 (MQ=255)
                                                                                                                          tAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACt              >  2:56281/1‑149 (MQ=255)
                                                                                                                          tAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACt              >  4:661473/1‑149 (MQ=255)
                                                                                                                           aaGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCttt                   >  3:510422/1‑143 (MQ=255)
                                                                                                                           aaGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCttt                   <  4:510422/143‑1 (MQ=255)
                                                                                                                             gAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc           <  3:152402/149‑1 (MQ=255)
                                                                                                                              aaaaaaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGTCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCTGACGAGGAGCCTTTGAACTACCa          >  4:590423/1‑149 (MQ=255)
                                                                                                                              aaaaaaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCa          >  2:447605/1‑149 (MQ=255)
                                                                                                                              aaaaaaCCCCGGAGCACGCCCCGGGGTTTTCGCTACAAATACAGTCATCTGATGCGGGAACATACTTGTCGCCTAACTGCTCTGATATGGTGTTACCCGGGTTCTGCGTACGAGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCa          <  3:742753/149‑1 (MQ=255)
                                                                                                                                    ccccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg    >  2:89543/1‑149 (MQ=255)
                                                                                                                                    ccccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg    >  4:593032/1‑149 (MQ=255)
                                                                                                                                    ccccGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg    >  3:315487/1‑149 (MQ=255)
                                                                                                                                     cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc   >  2:70787/1‑149 (MQ=255)
                                                                                                                                     cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc   >  2:661737/1‑149 (MQ=255)
                                                                                                                                      ccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc  >  4:321810/1‑149 (MQ=255)
                                                                                                                                      ccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc  >  2:495105/1‑149 (MQ=255)
                                                                                                                                                 cccGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCa          <  4:301651/130‑1 (MQ=255)
                                                                                                                                                 cccGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCa          >  3:301651/1‑130 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
GTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCC  >  NC_000913/3324814‑3325096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑