Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,334,704 | G→C | 7.2% | intergenic (+23/‑205) | ispB → / → sfsB | octaprenyl diphosphate synthase/malPQ operon transcriptional activator |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,334,704 | 0 | G | C | 7.2% | 459.4 / 20.5 | 140 | intergenic (+23/‑205) | ispB/sfsB | octaprenyl diphosphate synthase/malPQ operon transcriptional activator |
Reads supporting (aligned to +/- strand): ref base G (68/61); new base C (5/5); total (74/66) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 |
Only 100 of 142 total aligned reads displayed.
TGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATA > NC_000913/3334556‑3334848 | tGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGATACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAg > 3:205585/1‑149 (MQ=255) ggACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgc < 1:581860/149‑1 (MQ=255) tCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCggg > 1:99155/1‑149 (MQ=255) cAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACAACCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCATC‑GCGCGGGa > 4:491612/1‑149 (MQ=255) aGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGAt < 4:75420/149‑1 (MQ=255) cGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGATga < 4:877637/149‑2 (MQ=255) cGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGATga < 3:162528/149‑2 (MQ=255) gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACTCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATTCCCTCCCCGCATCCCGCGCAGC‑GCGCGGGATgag < 2:435880/149‑3 (MQ=255) gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑ACGCGGGATgag < 3:500064/149‑3 (MQ=255) gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa < 1:329828/149‑1 (MQ=255) gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa > 3:503166/1‑149 (MQ=255) gaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGtt < 3:118215/149‑1 (MQ=255) aagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTc < 1:595923/149‑1 (MQ=255) gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCT‑CCCACATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCAt > 1:473935/1‑149 (MQ=255) gACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaa > 3:303439/1‑149 (MQ=255) gACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaa > 3:827900/1‑149 (MQ=255) aCAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaa > 1:539083/1‑149 (MQ=255) aaaGCCATCGCAGCGTTACAGGTGCTCCCGGGCACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa > 4:791457/1‑149 (MQ=255) aaaGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa < 2:455066/149‑1 (MQ=255) aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca < 3:167399/149‑1 (MQ=255) gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac < 4:781216/149‑1 (MQ=255) gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac > 3:359656/1‑149 (MQ=255) ccATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACt > 3:343631/1‑149 (MQ=255) cATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATGCCCTCCCGTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTACCATAAAAACACtt > 1:643765/1‑149 (MQ=255) tCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAt > 3:181296/1‑149 (MQ=255) gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc < 4:827900/149‑1 (MQ=255) cGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGct < 3:712359/149‑1 (MQ=255) ttACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCGATAAAAACACTTATTCAGctct > 3:540953/1‑149 (MQ=255) ttACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGctct > 3:637578/1‑149 (MQ=255) tGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAacac > 2:380176/1‑149 (MQ=255) ccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaa > 1:705168/1‑149 (MQ=255) ggACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGa > 2:638799/1‑96 (MQ=255) ggACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGC‑GCTGCGCGGGa < 1:638799/96‑1 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg > 3:683160/1‑149 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg > 4:769340/1‑149 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg > 3:279248/1‑149 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg > 1:405819/1‑149 (MQ=255) ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa > 4:377294/1‑149 (MQ=255) ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa > 4:176490/1‑149 (MQ=255) tGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgt < 3:515134/149‑1 (MQ=255) gCGAGAAGCACTCATCGGCCTCGCACACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGc < 2:129621/149‑1 (MQ=255) gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCAt > 1:264811/1‑149 (MQ=255) gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCAt < 2:99155/149‑1 (MQ=255) agaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCT‑CCCACATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCt < 2:473935/149‑1 (MQ=255) aaGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCtt < 1:793720/149‑1 (MQ=255) aaGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCtt > 3:689297/1‑149 (MQ=255) gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta < 1:380176/149‑1 (MQ=255) aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata < 3:863150/149‑1 (MQ=255) aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata < 4:418645/149‑1 (MQ=255) aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCACATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata < 3:491612/149‑1 (MQ=255) cTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat < 4:343631/149‑1 (MQ=255) cTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat > 1:515588/1‑149 (MQ=255) tCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCTGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatata < 2:554167/149‑1 (MQ=255) tCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatata > 1:489104/1‑149 (MQ=255) tCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatata < 4:205585/149‑1 (MQ=255) aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt > 2:276552/1‑149 (MQ=255) aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt > 4:503341/1‑149 (MQ=255) ccTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGaa < 4:325289/149‑1 (MQ=255) cTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAat > 1:294133/1‑149 (MQ=255) gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa < 3:453671/118‑1 (MQ=255) gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa > 4:453671/1‑118 (MQ=255) gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGa > 4:170454/1‑144 (MQ=255) gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt < 2:405819/149‑1 (MQ=255) cacaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcac < 3:791457/149‑1 (MQ=255) acaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca > 1:48507/1‑149 (MQ=255) aTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca > 1:561304/1‑147 (MQ=255) aTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCACATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca < 2:561304/147‑1 (MQ=255) cGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACActct < 1:160629/149‑1 (MQ=255) gCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCtt < 1:546633/149‑1 (MQ=255) gCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCtt > 1:691602/1‑149 (MQ=255) tGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTa < 2:263453/149‑1 (MQ=255) tGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTa < 2:187682/149‑1 (MQ=255) cAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAgg > 1:142127/1‑149 (MQ=255) aaCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGa > 3:805467/1‑149 (MQ=255) aCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGaa < 2:515588/149‑1 (MQ=255) cgcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAAc < 2:489104/149‑1 (MQ=255) gATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttt > 1:143941/1‑149 (MQ=255) gATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttt > 3:517843/1‑149 (MQ=255) aTCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt < 4:49272/149‑1 (MQ=255) cGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTa < 2:680397/149‑1 (MQ=255) cGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTa < 4:668746/149‑1 (MQ=255) cGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTa < 4:49667/149‑1 (MQ=255) ttAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACTCCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTata < 4:794453/149‑3 (MQ=255) ttAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTaga < 4:279248/149‑1 (MQ=255) tcccctccccTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCaa > 4:678546/1‑149 (MQ=255) ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa > 3:401225/1‑149 (MQ=255) ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa < 3:790894/149‑1 (MQ=255) ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGGTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa > 4:275951/1‑149 (MQ=255) cctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa < 3:624019/85‑1 (MQ=255) cctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg > 1:860701/1‑149 (MQ=255) tcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGc > 2:785036/1‑149 (MQ=255) tcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCa < 4:503491/149‑1 (MQ=255) ccATCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTTAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCa > 3:290565/2‑149 (MQ=255) aTCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg > 4:242959/1‑149 (MQ=255) tCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa > 2:667750/1‑149 (MQ=255) tCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCGCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa > 2:768352/1‑149 (MQ=255) tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa > 1:427820/1‑149 (MQ=255) tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa < 4:372761/149‑1 (MQ=255) tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa > 2:876935/1‑149 (MQ=255) tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACA‑‑CTTTACAGGAACTTTTTAGAGCAATAGGCCATAAGGAGt > 4:461741/1‑149 (MQ=255) | TGGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATA > NC_000913/3334556‑3334848 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |