Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,388,285 | G→A | 7.3% | A281V (GCT→GTT) ‡ | aaeA ← | p‑hydroxybenzoic acid efflux system component |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,388,285 | 0 | G | A | 7.3% | 257.5 / 7.6 | 83 | A281V (GCT→GTT) ‡ | aaeA | p‑hydroxybenzoic acid efflux system component |
Reads supporting (aligned to +/- strand): ref base G (35/41); new base A (2/4); total (37/46) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 |
CCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCC > NC_000913/3388137‑3388433 | ccAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAg > 3:101130/1‑149 (MQ=255) ccAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAg > 2:136196/1‑149 (MQ=255) cAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGc > 1:385722/1‑149 (MQ=255) aGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCa < 1:673482/149‑1 (MQ=255) tttACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAgtg < 2:668865/149‑1 (MQ=255) tttACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAgtg < 4:2391/149‑1 (MQ=255) aCCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGCTA‑CAgtggtg < 3:314176/149‑1 (MQ=255) aCCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑CTACAgtggtg < 2:536167/149‑1 (MQ=255) ccGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑CTACAgtggtgg < 3:505239/149‑2 (MQ=255) ccGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑CTACAgtggtgg < 3:460338/149‑2 (MQ=255) aGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAg < 4:163422/149‑1 (MQ=255) gAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGcc > 3:198306/1‑149 (MQ=255) gAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGcc > 1:120423/1‑149 (MQ=255) aaTATGTTGGTTAGCAATCGAGAAAATACCCAGCGTGATTAACCAAAGTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAAACCTGGCTTTCGTCGCGGTCTTGTTTGCCAGTGACCACGGCTG‑TAGGAGTGGTGCCCGCAGGCCa < 4:629944/149‑1 (MQ=255) tatGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGa < 4:198306/149‑1 (MQ=255) tGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATg > 4:263087/1‑149 (MQ=255) aGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTg < 2:345384/149‑1 (MQ=255) tGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGt > 3:131314/1‑149 (MQ=255) ggAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGtt < 1:823802/149‑1 (MQ=255) ggAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGtt < 2:435154/149‑1 (MQ=255) gagaAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTg > 2:62852/1‑149 (MQ=255) gaAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTc < 3:231019/149‑1 (MQ=255) aaaTACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCga < 3:630474/149‑1 (MQ=255) tACCCACCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGAc < 2:390602/149‑1 (MQ=255) aCCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACg > 4:328826/1‑149 (MQ=255) ccATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGaa < 2:747077/149‑1 (MQ=255) ccATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGaa > 1:650375/1‑149 (MQ=255) cATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTCCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAAt > 1:160553/1‑149 (MQ=255) tCGTGCTTAACCAAACTCACGCAGGCGATGAGCCATGTTACGGAAGCACGAATCCTTCCTTTCGCCGCGATCTTGTTTGTCAGTGATAACCACTG‑TAGCAGGGGCGTTCGCAAGCCAGATGTTCTCTTGCTCGTTGTCGGGACGAATAc > 4:837128/1‑149 (MQ=255) tCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGTTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATAc < 4:694844/149‑1 (MQ=255) aCCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGaa > 2:141970/1‑149 (MQ=255) ccAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAac < 2:264093/149‑1 (MQ=255) ccAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAac > 4:200050/1‑149 (MQ=255) cACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgc < 4:77408/149‑1 (MQ=255) cAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCaa < 4:640389/149‑1 (MQ=255) aGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAAc < 1:285746/131‑1 (MQ=255) aGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAg > 1:428908/1‑149 (MQ=255) aGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTAGTTGTCGAGACGAATACGAAc > 2:285746/1‑131 (MQ=255) ggCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGa > 1:242529/1‑149 (MQ=255) gATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGc > 1:565070/1‑149 (MQ=255) gATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGc > 4:445919/1‑149 (MQ=255) gCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGa > 3:222844/1‑88 (MQ=255) gCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGa < 4:222844/88‑1 (MQ=255) ttttACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCa < 3:285837/149‑1 (MQ=255) ttttACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCa > 1:512692/1‑149 (MQ=255) tttACGGAAGCACGAATCCTGGCTTTTGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCaa < 1:641894/149‑1 (MQ=255) ttACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGTCGCACCCATTCAAg < 1:671013/149‑1 (MQ=255) ttACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAg < 3:121885/149‑1 (MQ=255) ttACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAg < 2:120423/149‑1 (MQ=255) ggAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTa > 3:86057/1‑149 (MQ=255) ggAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTa > 3:477172/1‑149 (MQ=255) acgaaCGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTaga < 1:436350/147‑1 (MQ=255) aagaaCGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTaga < 4:532248/149‑1 (MQ=255) aagaaCGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTaga < 2:497042/149‑1 (MQ=255) gaaCGAATCCTTGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGt > 3:704957/1‑149 (MQ=255) gaaCGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGt > 1:314412/1‑149 (MQ=255) aaTCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCtata < 3:660407/149‑1 (MQ=255) aaTCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCtata < 2:650375/149‑1 (MQ=255) tCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGaa > 2:691267/1‑94 (MQ=255) tCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGaa < 1:691267/94‑1 (MQ=255) tCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGt > 4:661812/1‑149 (MQ=255) ccTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTc < 3:661812/149‑1 (MQ=255) ccTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTc < 3:263087/149‑1 (MQ=255) tttCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTtc > 4:158055/1‑65 (MQ=255) tttCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTtc < 3:158055/65‑1 (MQ=255) tttCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATc > 4:510213/1‑149 (MQ=255) tttCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATc > 1:843602/1‑149 (MQ=255) gtcgCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCtt < 3:445919/149‑1 (MQ=255) gtcgCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCtt < 4:337993/149‑1 (MQ=255) tcgCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCttt < 4:86057/149‑1 (MQ=255) tcgCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCttt < 4:392484/149‑1 (MQ=255) tcgCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCttt < 1:578310/149‑1 (MQ=255) cgATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtc > 1:271110/1‑149 (MQ=255) cgATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtc < 4:84543/149‑1 (MQ=255) gATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcg > 4:531804/1‑149 (MQ=255) aTCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcgt > 4:691566/1‑149 (MQ=255) tCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcgtc < 3:604317/149‑1 (MQ=255) gCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGtgc > 1:823709/1‑149 (MQ=255) ggcaccacTG‑CTACAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGc > 1:862796/3‑149 (MQ=255) ggcaccacTG‑CTACAGTGGTGCCCGCAGGCCAGAGGTTCTCTTGCTGGTTGGCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGc > 2:703992/3‑149 (MQ=255) caccacTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGt > 4:398185/1‑149 (MQ=255) accacTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGtt > 3:59428/1‑149 (MQ=255) accacTG‑CTACAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGtt < 2:862796/149‑1 (MQ=255) ccacTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTg < 4:450118/149‑1 (MQ=255) cacTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTgg > 3:210439/1‑149 (MQ=255) gCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAcccc > 2:106085/1‑149 (MQ=255) | CCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTG‑TAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCC > NC_000913/3388137‑3388433 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |