Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,390,512 A→G 6.4% intergenic (+63/+71) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,390,5120AG 6.4% 336.9 / 7.7 108intergenic (+63/+71)aaeR/tldDtranscriptional regulator for aaeXAB operon/putative peptidase
Reads supporting (aligned to +/- strand):  ref base A (52/49);  new base G (4/3);  total (56/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 109 total aligned reads displayed.

GGTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTC  >  NC_000913/3390365‑3390660
                                                                                                                                                   |                                                                                                                                                    
ggTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGATGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCAc                                                                                                                                                     <  3:613962/149‑1 (MQ=255)
ggTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCAc                                                                                                                                                     <  3:581260/149‑1 (MQ=255)
 gTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGcc                                                                                                                                                    >  4:859717/1‑149 (MQ=255)
  tCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGccc                                                                                                                                                   >  2:810927/1‑149 (MQ=255)
  tCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCAccc                                                                                                                                                   >  1:157971/1‑149 (MQ=255)
  tCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCAccc                                                                                                                                                   >  3:619635/1‑149 (MQ=255)
    gTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCa                                                                                                                                                 >  3:339707/1‑149 (MQ=255)
     ggATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCaa                                                                                                                                                >  1:427867/2‑149 (MQ=255)
      gATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATCCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCaaa                                                                                                                                               <  1:156848/149‑1 (MQ=255)
       aTCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                              <  4:670929/149‑1 (MQ=255)
       aTCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                              >  3:381075/1‑149 (MQ=255)
       aTCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGCACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                              >  4:272626/1‑149 (MQ=255)
          aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAgggg                                                                                                                                           >  2:280444/1‑149 (MQ=255)
          aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAgggg                                                                                                                                           <  2:497051/149‑1 (MQ=255)
          aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAgggg                                                                                                                                           <  1:676251/149‑1 (MQ=255)
          aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                              <  1:524063/146‑1 (MQ=255)
          aaCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAg                                                                                                                                              >  2:524063/1‑146 (MQ=255)
           aCTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGc                                                                                                                                          >  2:66453/1‑149 (MQ=255)
               gCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgg                                                                                                                                      <  3:859717/149‑1 (MQ=255)
               gCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgg                                                                                                                                      <  3:661105/149‑1 (MQ=255)
               gCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAgg                                                                                                                                      <  3:479832/149‑1 (MQ=255)
                cTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAggg                                                                                                                                     >  1:891574/1‑149 (MQ=255)
                       attattTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggttag                                                                                                                                 >  3:656105/1‑142 (MQ=255)
                       attattTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGTGAgggttag                                                                                                                                 <  4:656105/146‑5 (MQ=255)
                       attattTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGtt                                                                                                                              <  3:272626/149‑1 (MQ=255)
                        ttattTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTc                                                                                                                             >  3:375630/1‑149 (MQ=255)
                         tattTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCg                                                                                                                            <  2:895161/149‑1 (MQ=255)
                           ttttGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTg                                                                                                                          >  2:255808/1‑149 (MQ=255)
                             ttgttgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCa                                                                                                                        <  4:284581/149‑1 (MQ=255)
                              tgttgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCAc                                                                                                                       <  4:672666/149‑1 (MQ=255)
                                ttgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGt                                                                                                                     >  4:348308/1‑149 (MQ=255)
                                          tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGatta                                                                                                           >  3:283679/1‑149 (MQ=255)
                                           aaaTTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGattaa                                                                                                          <  1:87911/149‑1 (MQ=255)
                                             aTTGTTTCAGGAGTTGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCAAAAGGTGAGAGGGGACCGTTCGTGCACGTAGAAAGattaatt                                                                                                        >  4:698884/1‑149 (MQ=255)
                                                gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                     >  2:792618/1‑149 (MQ=255)
                                                gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                     >  2:768419/1‑149 (MQ=255)
                                                 tttCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTAtac                                                                                                    >  4:752098/1‑147 (MQ=255)
                                                   tCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCtt                                                                                                  >  2:642575/1‑149 (MQ=255)
                                                     aGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCt                                                                                                <  1:724881/149‑1 (MQ=255)
                                                        agaTGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACACCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGaa                                                                                             <  1:586718/149‑1 (MQ=255)
                                                           ggCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGGACGTAGAAAGATTAATTATCCTTCTGAAAAt                                                                                          <  3:413205/148‑1 (MQ=255)
                                                            gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                         <  2:15526/149‑1 (MQ=255)
                                                            gCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATa                                                                                         <  3:369932/149‑1 (MQ=255)
                                                                 ggCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaa                                                                                    <  4:381075/149‑1 (MQ=255)
                                                                  gcgcGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGaaa                                                                                   <  1:66453/149‑1 (MQ=255)
                                                                        gAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAAttatta                                                                             <  1:642575/149‑1 (MQ=255)
                                                                        gAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAAttatta                                                                             <  1:255808/149‑1 (MQ=255)
                                                                            gagaAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCa                                                                         <  3:348308/149‑1 (MQ=255)
                                                                            gagaAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCa                                                                         >  2:301506/1‑149 (MQ=255)
                                                                            gagaAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCa                                                                         <  4:371840/149‑1 (MQ=255)
                                                                            gagaAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCa                                                                         <  3:265487/149‑1 (MQ=255)
                                                                                  tAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCg                                                                   >  2:533845/1‑149 (MQ=255)
                                                                                    aTTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGcc                                                                 >  2:500024/1‑149 (MQ=255)
                                                                                      tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGc                                                                                                                         >  2:470137/1‑91 (MQ=255)
                                                                                      tCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGc                                                                                                                         <  1:470137/91‑1 (MQ=255)
                                                                                       cATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAAc                                                              <  2:368592/149‑1 (MQ=255)
                                                                                           ttGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTc                                                          >  4:134409/1‑149 (MQ=255)
                                                                                            tGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCa                                                         <  2:885916/149‑1 (MQ=255)
                                                                                             gTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAg                                                        <  1:301506/149‑1 (MQ=255)
                                                                                             gTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAg                                                        <  4:346492/149‑1 (MQ=255)
                                                                                              tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAgg                                                       <  2:157971/149‑1 (MQ=255)
                                                                                               aCTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGt                                                      <  4:196349/149‑1 (MQ=255)
                                                                                                 tGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAAACCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTa                                                    >  3:503284/1‑149 (MQ=255)
                                                                                                   ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGCTTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                  <  2:348406/149‑1 (MQ=255)
                                                                                                   ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                  <  1:395978/149‑1 (MQ=255)
                                                                                                   ttACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATc                                                  >  1:248435/1‑149 (MQ=255)
                                                                                                       gtagTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGCAAATAGTGATATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACt                                              >  4:69836/4‑149 (MQ=255)
                                                                                                       gTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                       >  4:843815/1‑60 (MQ=255)
                                                                                                       gTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAg                                                                                                                                       <  3:843815/60‑1 (MQ=255)
                                                                                                           tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGACGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCa                                          >  3:728245/1‑149 (MQ=255)
                                                                                                              aaaCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACg                                       >  1:891203/1‑149 (MQ=255)
                                                                                                              aaaCCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACg                                       >  1:184077/1‑149 (MQ=255)
                                                                                                                 ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                    <  1:533845/149‑1 (MQ=255)
                                                                                                                   tgtgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggc                                  <  3:857631/149‑1 (MQ=255)
                                                                                                                    gtgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggct                                 <  4:339707/149‑1 (MQ=255)
                                                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctg                                >  3:445817/1‑149 (MQ=255)
                                                                                                                        cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGcc                             >  2:12717/1‑149 (MQ=255)
                                                                                                                        cAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGATCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTGCAACGTTGGCTGGcc                             >  1:694830/1‑149 (MQ=255)
                                                                                                                          aCGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCa                           >  1:382000/1‑149 (MQ=255)
                                                                                                                                  cccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAAcc                   <  4:307660/149‑1 (MQ=255)
                                                                                                                                   ccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCg                  >  1:862841/1‑149 (MQ=255)
                                                                                                                                    cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                    <  2:779476/130‑1 (MQ=255)
                                                                                                                                    cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtg                                    >  1:779476/1‑130 (MQ=255)
                                                                                                                                    cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                 >  4:74378/1‑149 (MQ=255)
                                                                                                                                    cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCgg                 >  1:389382/1‑149 (MQ=255)
                                                                                                                                     tCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGc                >  1:255396/1‑149 (MQ=255)
                                                                                                                                         ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTAt                                                   <  1:165984/110‑1 (MQ=255)
                                                                                                                                         ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTAt                                                   >  2:165984/1‑110 (MQ=255)
                                                                                                                                         ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAAc            >  3:691029/1‑149 (MQ=255)
                                                                                                                                         ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAAc            >  1:628366/1‑149 (MQ=255)
                                                                                                                                         ccTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAAc            >  2:561570/1‑149 (MQ=255)
                                                                                                                                            tccccTCGCCCCTTTGGGGTGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACttt         >  4:582466/3‑149 (MQ=255)
                                                                                                                                            aaCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGTCTGGCCCACGCCAACCGGCAAACttt         <  4:762158/149‑1 (MQ=255)
                                                                                                                                             aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACtttc        <  3:74378/149‑2 (MQ=255)
                                                                                                                                             aCCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTg        <  3:296085/149‑1 (MQ=255)
                                                                                                                                              cccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGc       >  3:317240/1‑149 (MQ=255)
                                                                                                                                              cccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGc       >  2:573161/1‑149 (MQ=255)
                                                                                                                                               ccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGt                                                           <  3:161994/96‑1 (MQ=255)
                                                                                                                                               ccTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGt                                                           >  4:161994/1‑96 (MQ=255)
                                                                                                                                                   aCCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCcttc  <  4:242141/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGTCGTGATCAACTCGCTGACGGATTATTTTGTTGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTC  >  NC_000913/3390365‑3390660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: