Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,740,791 G→C 5.5% G363R (GGC→CGC)  avtA → valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,740,7910GC 5.5% 1591.1 / 58.8 447G363R (GGC→CGC) avtAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
Reads supporting (aligned to +/- strand):  ref base G (196/226);  new base C (15/10);  total (211/236)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 460 total aligned reads displayed.

GTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGC  >  NC_000913/3740644‑3740939
                                                                                                                                                   |                                                                                                                                                    
gtTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACgcgg                                                                                                                                                     >  1:834371/1‑149 (MQ=255)
 ttCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACgcggc                                                                                                                                                    <  4:13290/149‑1 (MQ=255)
 ttCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACgcggc                                                                                                                                                    >  4:165230/1‑149 (MQ=255)
   cAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGt                                                                                                                                                  <  2:116365/149‑1 (MQ=255)
    aGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTg                                                                                                                                                 >  2:378598/1‑149 (MQ=255)
     ggAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGc                                                                                                                                                <  2:331576/149‑1 (MQ=255)
     ggAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGc                                                                                                                                                <  3:404247/149‑1 (MQ=255)
      gAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCt                                                                                                                                               >  2:821195/1‑149 (MQ=255)
         aCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGAt                                                                                                                                            >  1:448884/1‑149 (MQ=255)
         aCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGAt                                                                                                                                            <  1:585385/149‑1 (MQ=255)
         aCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGAt                                                                                                                                            >  3:357227/1‑149 (MQ=255)
         aCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCCGCGTGCtttc                                                                                                                                            <  1:855820/149‑4 (MQ=255)
         aCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCCGCGTGCtttc                                                                                                                                            <  1:759401/149‑4 (MQ=255)
                ccgtcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCg                                                                                                                                     >  2:22352/4‑149 (MQ=255)
                ccATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCg                                                                                                                                     >  3:99226/1‑149 (MQ=255)
                ccATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCg                                                                                                                                     >  3:389915/1‑149 (MQ=255)
                 catcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg                                                                                                                                    <  4:135467/149‑1 (MQ=255)
                 catcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg                                                                                                                                    >  4:833212/1‑149 (MQ=255)
                 catcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg                                                                                                                                    >  2:34637/1‑149 (MQ=255)
                   tcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGCTTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAATCACGCGGCGTGCTGATGGTGCCgggg                                                                                                                                  <  4:62359/149‑1 (MQ=255)
                   tcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgggg                                                                                                                                  <  2:508415/149‑1 (MQ=255)
                     atTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGca                                                                                                                                <  4:297958/149‑1 (MQ=255)
                          ccgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAAct                                                                                                                           >  2:204347/1‑149 (MQ=255)
                          ccgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTCGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCCGCGTGCTGATGGTGCCGGGGCACAAct                                                                                                                           >  2:796643/1‑149 (MQ=255)
                            gcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActtc                                                                                                                         >  1:524222/1‑149 (MQ=255)
                                tttACCGGAAAATCGCTGCCTGATTCATAAACTGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTcc                                                                                                                     <  3:743007/149‑1 (MQ=255)
                                   aCCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAg                                                                                                                  <  2:105463/149‑1 (MQ=255)
                                     cGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgggg                                                                                                                                  <  4:792201/131‑1 (MQ=255)
                                      ggAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActtc                                                                                                                         <  2:553635/139‑1 (MQ=255)
                                       gAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCt                                                                                                              <  1:208037/149‑1 (MQ=255)
                                        aaaaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTGTGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTg                                                                                                             >  1:343006/1‑149 (MQ=255)
                                          aaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGa                                                                                                           <  3:633185/149‑1 (MQ=255)
                                           aTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGAt                                                                                                          >  4:337973/1‑149 (MQ=255)
                                                tGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGcac                                                                                                                               >  1:686051/1‑123 (MQ=255)
                                                   cTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTg                                                                                                  >  2:439438/1‑149 (MQ=255)
                                                   cTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTg                                                                                                  >  2:772108/1‑149 (MQ=255)
                                                   cTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTg                                                                                                  <  1:894509/149‑1 (MQ=255)
                                                    tGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAAc                                                                                                                            <  4:280217/122‑1 (MQ=255)
                                                    tGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTgg                                                                                                 <  3:69755/149‑1 (MQ=255)
                                                     gATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAc                                                                                                      >  3:567872/1‑143 (MQ=255)
                                                           tAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATa                                                                                          <  3:665979/149‑1 (MQ=255)
                                                            aaaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATAc                                                                                         <  1:102208/149‑1 (MQ=255)
                                                             aaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACg                                                                                        >  4:345868/1‑149 (MQ=255)
                                                              aCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAACGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGc                                                                                       <  4:880234/149‑1 (MQ=255)
                                                                 ggAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATc                                                                                    <  4:389915/149‑1 (MQ=255)
                                                                   aaGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTCTCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCaa                                                                                  <  1:639536/149‑1 (MQ=255)
                                                                   aaGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCaa                                                                                  <  3:517266/149‑1 (MQ=255)
                                                                    aGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAAt                                                                                 <  1:315742/149‑1 (MQ=255)
                                                                    aGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAAt                                                                                 <  4:71283/149‑1 (MQ=255)
                                                                      gAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGt                                                                               >  3:33882/1‑149 (MQ=255)
                                                                       aGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTa                                                                              >  1:361128/1‑149 (MQ=255)
                                                                       aGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTa                                                                              >  3:711916/1‑149 (MQ=255)
                                                                           aTTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATgcg                                                                          >  4:572758/1‑149 (MQ=255)
                                                                               tCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATg                                                                      <  2:393112/149‑1 (MQ=255)
                                                                                 ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                    >  4:657098/1‑149 (MQ=255)
                                                                                 ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                    <  1:612259/149‑1 (MQ=255)
                                                                                  tctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAAc                                                                   <  3:165230/149‑1 (MQ=255)
                                                                                   ctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGcccc                                                                                                                                    <  3:254533/83‑3 (MQ=255)
                                                                                     ggCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTAc                                                                <  1:433628/149‑1 (MQ=255)
                                                                                      gCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACg                                                               >  4:419721/1‑149 (MQ=255)
                                                                                      gCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACg                                                               >  4:206865/1‑149 (MQ=255)
                                                                                        tATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTa                                                             >  3:795008/1‑149 (MQ=255)
                                                                                               tAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGc                                                      >  2:699282/1‑149 (MQ=255)
                                                                                                  ggATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                   >  2:837677/1‑149 (MQ=255)
                                                                                                   gATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGga                                                  <  2:882056/149‑1 (MQ=255)
                                                                                                          ccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAAtt                                           <  3:777619/149‑1 (MQ=255)
                                                                                                           cATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                   >  3:231548/1‑140 (MQ=255)
                                                                                                           cATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                   <  2:165613/140‑1 (MQ=255)
                                                                                                            aTTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGa                                         <  1:169488/149‑1 (MQ=255)
                                                                                                             ttACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAg                                        <  4:184819/149‑1 (MQ=255)
                                                                                                             ttACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAg                                        <  3:130878/149‑1 (MQ=255)
                                                                                                               aCGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGTGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGc                                      <  4:733228/149‑1 (MQ=255)
                                                                                                                   ccAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCgggg                                  <  2:568277/149‑1 (MQ=255)
                                                                                                                      agcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTAc                                                            >  4:477810/1‑120 (MQ=255)
                                                                                                                      agcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTAc                                                            <  3:477810/120‑1 (MQ=255)
                                                                                                                      agcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                   <  3:280894/129‑1 (MQ=255)
                                                                                                                      agcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggggggg                               >  2:14213/1‑147 (MQ=255)
                                                                                                                       gcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCgggg                                  >  1:730209/1‑145 (MQ=255)
                                                                                                                       gcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTATTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCgggg                                  >  2:732430/1‑145 (MQ=255)
                                                                                                                          gcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAg                                        <  1:252404/136‑1 (MQ=255)
                                                                                                                          gcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGa                           <  2:277949/149‑1 (MQ=255)
                                                                                                                            tctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAtt                         >  2:482421/1‑149 (MQ=255)
                                                                                                                              tATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCt                       >  1:547950/1‑149 (MQ=255)
                                                                                                                                tCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTgg                     >  1:124643/1‑149 (MQ=255)
                                                                                                                                 cAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGc                    <  1:796643/149‑1 (MQ=255)
                                                                                                                                   gcgcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCgg                  >  3:201742/1‑149 (MQ=255)
                                                                                                                                     gcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGaa                <  1:273521/149‑1 (MQ=255)
                                                                                                                                      ccTGAGAGCACGCGGAGTGCTGATGGTGCCGGCGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGGATACGCATCAAGGTATGCGCATGAAGTACGTACCAGAGCCGGAGAAAATGGAGGCGGGGGGGAAGATCCTGGCGGAag               <  4:373341/149‑1 (MQ=255)
                                                                                                                                      accatcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGaa                <  1:484174/142‑1 (MQ=255)
                                                                                                                                      accatcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAag               >  3:855473/7‑149 (MQ=255)
                                                                                                                                       cTGAAAGCACGCGGCGTGCTGATGGTGCCGGTGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCAGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGAGAAGATTCTGGCGGgaga              >  2:359804/1‑149 (MQ=255)
                                                                                                                                       cTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGa                                         >  1:236464/1‑122 (MQ=255)
                                                                                                                                         gAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAgaga            <  4:253800/149‑1 (MQ=255)
                                                                                                                                         gAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAgaga            <  4:676006/149‑1 (MQ=255)
                                                                                                                                         atccgCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAgaga            >  1:415233/5‑149 (MQ=255)
                                                                                                                                           aaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATa          <  2:247935/149‑1 (MQ=255)
                                                                                                                                            aGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAg         >  2:856149/1‑149 (MQ=255)
                                                                                                                                               aCGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaaa      <  2:685370/149‑1 (MQ=255)
                                                                                                                                                 gcggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAaga    <  3:426538/149‑1 (MQ=255)
                                                                                                                                                 gcggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAaga    <  2:622631/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGC  >  NC_000913/3740644‑3740939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: