Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,028,482 T→G 5.3% A97A (GCA→GCC) ‡ fadA ← 3‑ketoacyl‑CoA thiolase (thiolase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,028,4820TG 5.3% 305.1 / 5.9 89A97A (GCA→GCC) ‡fadA3‑ketoacyl‑CoA thiolase (thiolase I)
Reads supporting (aligned to +/- strand):  ref base T (36/48);  new base G (3/2);  total (39/50)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCC  >  NC_000913/4028334‑4028629
                                                                                                                                                    |                                                                                                                                                   
tcatGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGt                                                                                                                                                     >  2:267987/1‑149 (MQ=255)
tcatGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGt                                                                                                                                                     <  3:828024/149‑1 (MQ=255)
   tGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCGTTCGTGCTGCGTCATGCAGTGcc                                                                                                                                                  >  4:390168/1‑149 (MQ=255)
    gCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCt                                                                                                                                                 <  2:638761/149‑1 (MQ=255)
      ccgccgCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGc                                                                                                                                               >  2:277087/1‑149 (MQ=255)
        gccgagttGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGGCATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCAt                                                                                                                                             <  4:675873/143‑1 (MQ=255)
          cgcTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGCACTGCATga                                                                                                                                           <  3:563785/149‑2 (MQ=255)
            cTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGAt                                                                                                                                         <  2:503630/149‑1 (MQ=255)
            cTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGAt                                                                                                                                         <  1:15901/149‑1 (MQ=255)
               tGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGaa                                                                                                                                      <  4:229071/149‑1 (MQ=255)
                   gACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                          >  3:206444/1‑141 (MQ=255)
                   gACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                          <  4:206444/141‑1 (MQ=255)
                   gACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACcac                                                                                                                                  >  4:79566/1‑149 (MQ=255)
                     cATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAGCcacac                                                                                                                                <  1:267987/149‑1 (MQ=255)
                       ttGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACaa                                                                                                                              <  2:355589/149‑1 (MQ=255)
                       ttGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATAGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACaa                                                                                                                              >  2:834881/1‑149 (MQ=255)
                        tGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAg                                                                                                                             <  1:332022/149‑1 (MQ=255)
                         gCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                          >  3:553066/1‑135 (MQ=255)
                         gCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                          <  4:553066/135‑1 (MQ=255)
                         gCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGc                                                                                                                            <  2:234655/149‑1 (MQ=255)
                           ggCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGa                                                                                                                          >  2:622392/1‑149 (MQ=255)
                            gCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGc                                                                                                                                                   >  1:679447/1‑123 (MQ=255)
                            gCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGc                                                                                                                                                   <  2:679447/123‑1 (MQ=255)
                            gCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGAt                                                                                                                         <  4:497841/149‑1 (MQ=255)
                               cAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCCGCATGGATGAACCACACAAGCGATTaa                                                                                                                      <  3:590393/149‑1 (MQ=255)
                                 ggCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACg                                                                                                                    <  2:96665/149‑1 (MQ=255)
                                 ggCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGCACTGCATga                                                                                                                                           >  1:574567/1‑125 (MQ=255)
                                 ggCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGCACTGCATga                                                                                                                                           <  2:574567/126‑2 (MQ=255)
                                  gCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACgg                                                                                                                   <  1:622392/149‑1 (MQ=255)
                                         tGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAAccgc                                                                                                            >  4:261270/1‑149 (MQ=255)
                                               tCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGAc                                                                                                      >  2:811517/1‑149 (MQ=255)
                                                  aCGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACaga                                                                                                   <  1:149380/149‑1 (MQ=255)
                                                   cGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACagag                                                                                                  <  4:589160/149‑1 (MQ=255)
                                                     ccGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtg                                                                                                <  3:58013/149‑1 (MQ=255)
                                                      cGTGACTCACCGGCACATGGCCCATATGCGCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtgt                                                                                               <  2:218005/149‑1 (MQ=255)
                                                       gTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGtgtg                                                                                              <  3:421217/149‑1 (MQ=255)
                                                       gTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGtgtg                                                                                              <  1:677550/149‑1 (MQ=255)
                                                        tGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCGTTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTgg                                                                                             <  3:390168/149‑1 (MQ=255)
                                                        tGACTAATCGGCACATGGCGCATACGCTCGACGCGGACAACCAGATAGGCCTGCGGCGCACCAGCCATGATCATTCGTGCTGCGTCATGCAGTGCGTGGATGGATTGACCACATAAGCGATTAAACGGAACCGCCGGGACAGAGTGTgg                                                                                             <  4:607647/149‑1 (MQ=255)
                                                              aTCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGaa                                                                                                                                      >  2:6432/1‑102 (MQ=255)
                                                              aTCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGaa                                                                                                                                      <  1:6432/102‑1 (MQ=255)
                                                               tCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCGGGGACAGAGTGTGGTACTTCt                                                                                      <  4:755552/149‑1 (MQ=255)
                                                               tCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCt                                                                                      <  4:384371/149‑1 (MQ=255)
                                                               tCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCt                                                                                      <  3:92772/149‑1 (MQ=255)
                                                                  gCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGAGAGAGTGTGGTACTTCTGcc                                                                                   <  3:517942/149‑1 (MQ=255)
                                                                      aTGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACg                                                                                                                    >  2:858686/1‑112 (MQ=255)
                                                                      aTGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACg                                                                                                                    <  1:858686/112‑1 (MQ=255)
                                                                             atatGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGcgccgc                                                                        >  2:874636/1‑149 (MQ=255)
                                                                                   tCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                                  >  3:145719/1‑149 (MQ=255)
                                                                                   tCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                                  >  2:88078/1‑149 (MQ=255)
                                                                                   tCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                                  >  1:686691/1‑149 (MQ=255)
                                                                                    ccACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACgg                                                                 <  1:811517/149‑1 (MQ=255)
                                                                                      aCGCCGCCAACCAGACATGCCTGCGCATGGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                               >  3:758413/1‑149 (MQ=255)
                                                                                      aCGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                               >  1:754960/1‑149 (MQ=255)
                                                                                         ccgccAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGat                                                            <  4:710519/149‑1 (MQ=255)
                                                                                                 taGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGACATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAAc                                                    <  3:79566/148‑1 (MQ=255)
                                                                                                  aGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAAcc                                                   <  4:90050/149‑1 (MQ=255)
                                                                                                          cTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCa                                           <  1:483004/149‑1 (MQ=255)
                                                                                                           tGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAg                                          >  4:268582/1‑149 (MQ=255)
                                                                                                                aTCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTct                                     >  3:26468/1‑149 (MQ=255)
                                                                                                                  cGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgc                                   <  4:758413/149‑1 (MQ=255)
                                                                                                                    ccAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGGGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgctg                                 >  2:465024/1‑149 (MQ=255)
                                                                                                                       gTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGcac                              <  3:358296/149‑1 (MQ=255)
                                                                                                                          aTGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgc                                   <  3:206446/141‑1 (MQ=255)
                                                                                                                          aTGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgc                                   >  4:206446/1‑141 (MQ=255)
                                                                                                                           tGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACaa                          >  2:194691/1‑149 (MQ=255)
                                                                                                                           tGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCATGCTCCAGCGTCTGCTGCACACaa                          >  2:866028/1‑149 (MQ=255)
                                                                                                                            gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGc                                               >  3:679244/1‑127 (MQ=255)
                                                                                                                            gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGc                                               <  4:679244/127‑1 (MQ=255)
                                                                                                                            gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                         <  2:370691/149‑1 (MQ=255)
                                                                                                                            gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                         <  3:541708/149‑1 (MQ=255)
                                                                                                                            gATCATTCGTACTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                         <  1:300081/149‑1 (MQ=255)
                                                                                                                             aTCATTCGTGCTGCGTCCTGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAcc                        >  4:342825/1‑149 (MQ=255)
                                                                                                                             aTCATTCGTGCTAAGTCATGAAGTGCCTGAATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTCCTTCTTCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAcc                        >  4:592666/1‑149 (MQ=255)
                                                                                                                              tCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAccc                       <  3:115521/149‑1 (MQ=255)
                                                                                                                              tCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAccc                       <  1:180767/149‑1 (MQ=255)
                                                                                                                                aTTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCa                     >  4:644596/1‑149 (MQ=255)
                                                                                                                                 ttCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCTTGCTCCAGCGTCTGCTGCACACAAccc                       >  1:736319/1‑146 (MQ=255)
                                                                                                                                 ttCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAccc                       <  2:736319/146‑1 (MQ=255)
                                                                                                                                 ttCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAg                    >  4:455585/1‑149 (MQ=255)
                                                                                                                                        tgcGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAatat             >  1:51561/1‑149 (MQ=255)
                                                                                                                                         gcGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtc            <  3:722813/149‑1 (MQ=255)
                                                                                                                                           tccATGCAGGCACTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtcgt          >  2:287509/3‑149 (MQ=255)
                                                                                                                                            ccATGCAGGCACTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtcgtc         >  1:301621/2‑149 (MQ=255)
                                                                                                                                              aTGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGa       <  2:340510/149‑1 (MQ=255)
                                                                                                                                               tGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAg      >  2:333364/1‑149 (MQ=255)
                                                                                                                                                gCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAgg     >  2:316411/1‑149 (MQ=255)
                                                                                                                                                gCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAgg     <  1:10307/149‑1 (MQ=255)
                                                                                                                                                  aGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCCGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGgc   >  4:521154/1‑149 (MQ=255)
                                                                                                                                                   gTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGgcc  >  4:588050/1‑149 (MQ=255)
                                                                                                                                                   gTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCACAGTAAATATCGTCGAGGgcc  >  3:280026/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCC  >  NC_000913/4028334‑4028629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑