Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,028,484 C→A 5.8% A97S (GCA→TCA) ‡ fadA ← 3‑ketoacyl‑CoA thiolase (thiolase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,028,4840CA 5.8% 294.4 / 7.0 86A97S (GCA→TCA) ‡fadA3‑ketoacyl‑CoA thiolase (thiolase I)
Reads supporting (aligned to +/- strand):  ref base C (34/47);  new base A (3/2);  total (37/49)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

TGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCC  >  NC_000913/4028337‑4028632
                                                                                                                                                   |                                                                                                                                                    
tGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCGTTCGTGCTGCGTCATGCAGTGcc                                                                                                                                                     >  4:390168/1‑149 (MQ=255)
 gCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCt                                                                                                                                                    <  2:638761/149‑1 (MQ=255)
   ccgccgCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGc                                                                                                                                                  >  2:277087/1‑149 (MQ=255)
     gccgagttGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGGCATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCAt                                                                                                                                                <  4:675873/143‑1 (MQ=255)
       cgcTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGCACTGCATga                                                                                                                                              <  3:563785/149‑2 (MQ=255)
         cTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGAt                                                                                                                                            <  1:15901/149‑1 (MQ=255)
         cTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGAt                                                                                                                                            <  2:503630/149‑1 (MQ=255)
            tGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGaa                                                                                                                                         <  4:229071/149‑1 (MQ=255)
                gACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                             >  3:206444/1‑141 (MQ=255)
                gACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                             <  4:206444/141‑1 (MQ=255)
                gACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACcac                                                                                                                                     >  4:79566/1‑149 (MQ=255)
                  cATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAGCcacac                                                                                                                                   <  1:267987/149‑1 (MQ=255)
                    ttGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACaa                                                                                                                                 <  2:355589/149‑1 (MQ=255)
                    ttGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATAGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACaa                                                                                                                                 >  2:834881/1‑149 (MQ=255)
                     tGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAg                                                                                                                                <  1:332022/149‑1 (MQ=255)
                      gCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                             >  3:553066/1‑135 (MQ=255)
                      gCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGa                                                                                                                                             <  4:553066/135‑1 (MQ=255)
                      gCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGc                                                                                                                               <  2:234655/149‑1 (MQ=255)
                        ggCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGa                                                                                                                             >  2:622392/1‑149 (MQ=255)
                         gCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGc                                                                                                                                                      <  2:679447/123‑1 (MQ=255)
                         gCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGc                                                                                                                                                      >  1:679447/1‑123 (MQ=255)
                         gCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGAt                                                                                                                            <  4:497841/149‑1 (MQ=255)
                            cAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCCGCATGGATGAACCACACAAGCGATTaa                                                                                                                         <  3:590393/149‑1 (MQ=255)
                              ggCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACg                                                                                                                       <  2:96665/149‑1 (MQ=255)
                              ggCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGCACTGCATga                                                                                                                                              <  2:574567/126‑2 (MQ=255)
                              ggCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGGCACTGCATga                                                                                                                                              >  1:574567/1‑125 (MQ=255)
                               gCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACgg                                                                                                                      <  1:622392/149‑1 (MQ=255)
                                      tGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAAccgc                                                                                                               >  4:261270/1‑149 (MQ=255)
                                            tCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGAc                                                                                                         >  2:811517/1‑149 (MQ=255)
                                               aCGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACaga                                                                                                      <  1:149380/149‑1 (MQ=255)
                                                cGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACagag                                                                                                     <  4:589160/149‑1 (MQ=255)
                                                  ccGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtg                                                                                                   <  3:58013/149‑1 (MQ=255)
                                                   cGTGACTCACCGGCACATGGCCCATATGCGCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAgtgt                                                                                                  <  2:218005/149‑1 (MQ=255)
                                                    gTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGtgtg                                                                                                 <  3:421217/149‑1 (MQ=255)
                                                    gTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGtgtg                                                                                                 <  1:677550/149‑1 (MQ=255)
                                                     tGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCGTTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTgg                                                                                                <  3:390168/149‑1 (MQ=255)
                                                     tGACTAATCGGCACATGGCGCATACGCTCGACGCGGACAACCAGATAGGCCTGCGGCGCACCAGCCATGATCATTCGTGCTGCGTCATGCAGTGCGTGGATGGATTGACCACATAAGCGATTAAACGGAACCGCCGGGACAGAGTGTgg                                                                                                <  4:607647/149‑1 (MQ=255)
                                                           aTCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGaa                                                                                                                                         >  2:6432/1‑102 (MQ=255)
                                                           aTCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGaa                                                                                                                                         <  1:6432/102‑1 (MQ=255)
                                                            tCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCGGGGACAGAGTGTGGTACTTCt                                                                                         <  4:755552/149‑1 (MQ=255)
                                                            tCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCt                                                                                         <  4:384371/149‑1 (MQ=255)
                                                            tCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCt                                                                                         <  3:92772/149‑1 (MQ=255)
                                                               gCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGAGAGAGTGTGGTACTTCTGcc                                                                                      <  3:517942/149‑1 (MQ=255)
                                                                   aTGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACg                                                                                                                       >  2:858686/1‑112 (MQ=255)
                                                                   aTGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACg                                                                                                                       <  1:858686/112‑1 (MQ=255)
                                                                          atatGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGcgccgc                                                                           >  2:874636/1‑149 (MQ=255)
                                                                                tCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                                     >  2:88078/1‑149 (MQ=255)
                                                                                tCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                                     >  3:145719/1‑149 (MQ=255)
                                                                                tCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACg                                                                     >  1:686691/1‑149 (MQ=255)
                                                                                 ccACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACgg                                                                    <  1:811517/149‑1 (MQ=255)
                                                                                   aCGCCGCCAACCAGACATGCCTGCGCATGGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                                  >  3:758413/1‑149 (MQ=255)
                                                                                   aCGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGc                                                                  >  1:754960/1‑149 (MQ=255)
                                                                                      ccgccAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGat                                                               <  4:710519/149‑1 (MQ=255)
                                                                                              taGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGACATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAAc                                                       <  3:79566/148‑1 (MQ=255)
                                                                                               aGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAAcc                                                      <  4:90050/149‑1 (MQ=255)
                                                                                                       cTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCa                                              <  1:483004/149‑1 (MQ=255)
                                                                                                        tGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAg                                             >  4:268582/1‑149 (MQ=255)
                                                                                                             aTCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTct                                        >  3:26468/1‑149 (MQ=255)
                                                                                                               cGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgc                                      <  4:758413/149‑1 (MQ=255)
                                                                                                                 ccAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGGGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgctg                                    >  2:465024/1‑149 (MQ=255)
                                                                                                                    gTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGcac                                 <  3:358296/149‑1 (MQ=255)
                                                                                                                       aTGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgc                                      <  3:206446/141‑1 (MQ=255)
                                                                                                                       aTGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTctgc                                      >  4:206446/1‑141 (MQ=255)
                                                                                                                        tGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACaa                             >  2:194691/1‑149 (MQ=255)
                                                                                                                        tGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCATGCTCCAGCGTCTGCTGCACACaa                             >  2:866028/1‑149 (MQ=255)
                                                                                                                         gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGc                                                  >  3:679244/1‑127 (MQ=255)
                                                                                                                         gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGc                                                  <  4:679244/127‑1 (MQ=255)
                                                                                                                         gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                            <  2:370691/149‑1 (MQ=255)
                                                                                                                         gATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                            <  3:541708/149‑1 (MQ=255)
                                                                                                                         gATCATTCGTACTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAc                            <  1:300081/149‑1 (MQ=255)
                                                                                                                          aTCATTCGTGCTGCGTCCTGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAcc                           >  4:342825/1‑149 (MQ=255)
                                                                                                                          aTCATTCGTGCTAAGTCATGAAGTGCCTGAATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTCCTTCTTCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAcc                           >  4:592666/1‑149 (MQ=255)
                                                                                                                           tCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAccc                          <  1:180767/149‑1 (MQ=255)
                                                                                                                           tCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAccc                          <  3:115521/149‑1 (MQ=255)
                                                                                                                             aTTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCa                        >  4:644596/1‑149 (MQ=255)
                                                                                                                              ttCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCTTGCTCCAGCGTCTGCTGCACACAAccc                          >  1:736319/1‑146 (MQ=255)
                                                                                                                              ttCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAAccc                          <  2:736319/146‑1 (MQ=255)
                                                                                                                              ttCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAg                       >  4:455585/1‑149 (MQ=255)
                                                                                                                                     tgcGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAatat                >  1:51561/1‑149 (MQ=255)
                                                                                                                                      gcGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtc               <  3:722813/149‑1 (MQ=255)
                                                                                                                                        tccATGCAGGCACTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtcgt             >  2:287509/3‑149 (MQ=255)
                                                                                                                                         ccATGCAGGCACTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATAtcgtc            >  1:301621/2‑149 (MQ=255)
                                                                                                                                           aTGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGa          <  2:340510/149‑1 (MQ=255)
                                                                                                                                            tGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAg         >  2:333364/1‑149 (MQ=255)
                                                                                                                                             gCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAgg        >  2:316411/1‑149 (MQ=255)
                                                                                                                                             gCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAgg        <  1:10307/149‑1 (MQ=255)
                                                                                                                                               aGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCCGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGgc      >  4:521154/1‑149 (MQ=255)
                                                                                                                                                gTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGgcc     >  4:588050/1‑149 (MQ=255)
                                                                                                                                                gTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCACAGTAAATATCGTCGAGGgcc     >  3:280026/1‑149 (MQ=255)
                                                                                                                                                   ccTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGgccgcc  <  3:640724/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCCTGCGCATCGCCAGTCATGATCATTCGTGCTGCGTCATGCAGTGCCTGCATGGATGAACCACACAAGCGATTAACGGTAACCGCCGGGACAGAGTGTGGTACTTCTGCCAGCAGCGCCGCGTTACGGGCGATATTAAAACCCTGCTCCAGCGTCTGCTGCACACAACCCCAGTAAATATCGTCGAGGGCCGCC  >  NC_000913/4028337‑4028632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑