Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,371,932 A→C 5.4% E303A (GAA→GCA)  groL → Cpn60 chaperonin GroEL, large subunit of GroESL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,371,9320AC 5.4% 429.5 / 12.9 124E303A (GAA→GCA) groLCpn60 chaperonin GroEL, large subunit of GroESL
Reads supporting (aligned to +/- strand):  ref base A (50/67);  new base C (5/2);  total (55/69)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 128 total aligned reads displayed.

TAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCA  >  NC_000913/4371785‑4372079
                                                                                                                                                   |                                                                                                                                                   
tAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGaa                                                                                                                                                    <  4:128307/149‑1 (MQ=255)
tAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGaa                                                                                                                                                    >  3:175991/1‑149 (MQ=255)
 aGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAag                                                                                                                                                   >  3:658820/1‑149 (MQ=255)
  gAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTGGGCGATCGTCGTAAAGCTATGCTGCAGGATATCACAACCCTGACTGGCGGTACCGTGATCTCTGAaga                                                                                                                                                  >  2:526969/1‑149 (MQ=255)
  gAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAaga                                                                                                                                                  <  2:13887/149‑1 (MQ=255)
        gAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTTCAGAGATcac                                                                                                                                            <  3:863690/149‑3 (MQ=255)
        gAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCgg                                                                                                                                            >  2:89880/1‑149 (MQ=255)
          aGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTa                                                                                                                                          <  1:833132/149‑1 (MQ=255)
           gcgcTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTTCAGAGATcacggt                                                                                                                                         <  3:56824/149‑6 (MQ=255)
            cgcTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATg                                                                                                                                        <  4:641404/149‑1 (MQ=255)
            cgcTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATg                                                                                                                                        <  1:200786/149‑1 (MQ=255)
              cTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGa                                                                                                                                      <  1:592053/149‑1 (MQ=255)
                ggCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGc                                                                                                                                    <  2:213797/149‑1 (MQ=255)
                 gCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCt                                                                                                                                   <  4:583450/149‑1 (MQ=255)
                 gCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCt                                                                                                                                   <  2:290262/149‑1 (MQ=255)
                  cAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTg                                                                                                                                  <  4:658820/149‑1 (MQ=255)
                     ctctGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGaa                                                                                                                               <  3:507046/149‑1 (MQ=255)
                      tctGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGaaa                                                                                                                              >  1:843077/1‑149 (MQ=255)
                      tctGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGaaa                                                                                                                              <  3:279082/149‑1 (MQ=255)
                        tGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGaaaaa                                                                                                                            <  2:68326/149‑1 (MQ=255)
                        tGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGaaaaa                                                                                                                            <  4:754317/149‑1 (MQ=255)
                        tGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGaaaaa                                                                                                                            <  3:442838/149‑1 (MQ=255)
                          gttgttAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGc                                                                                                                          >  1:849527/1‑149 (MQ=255)
                           ttgttAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCa                                                                                                                         >  2:357186/1‑149 (MQ=255)
                           ttgttAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCa                                                                                                                         >  3:299737/1‑149 (MQ=255)
                            tgttAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGTTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATAGGTATGGAGCTGGAAAAATCaa                                                                                                                        <  4:371467/149‑1 (MQ=255)
                            tgttAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCaa                                                                                                                        <  4:812740/149‑1 (MQ=255)
                            tgttAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCaa                                                                                                                        <  4:38865/149‑1 (MQ=255)
                             gttAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAAc                                                                                                                       <  2:88654/149‑1 (MQ=255)
                                aaCACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTa                                                                                                                                          <  1:294963/127‑1 (MQ=255)
                                aaCACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTa                                                                                                                                          >  2:294963/1‑127 (MQ=255)
                                aaCACCATGCATGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCt                                                                                                                    >  4:198530/1‑149 (MQ=255)
                                  cacCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTgg                                                                                                                  >  4:772302/1‑149 (MQ=255)
                                     cATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGCAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAg                                                                                                               >  4:72303/1‑149 (MQ=255)
                                     cATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAg                                                                                                               >  2:550308/1‑149 (MQ=255)
                                      aTGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAGCAACCCTGGAAGAc                                                                                                             <  1:539250/149‑1 (MQ=255)
                                        gCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGAcc                                                                                                            >  4:187311/1‑149 (MQ=255)
                                         cGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCt                                                                                                           <  1:89880/149‑1 (MQ=255)
                                         cGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCt                                                                                                           <  2:626698/149‑1 (MQ=255)
                                          gTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTg                                                                                                          <  1:550308/149‑1 (MQ=255)
                                          gTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAAACCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTg                                                                                                          >  1:131524/1‑149 (MQ=255)
                                           tGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTgg                                                                                                         <  1:163481/149‑1 (MQ=255)
                                           tGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTgg                                                                                                         <  4:141750/149‑1 (MQ=255)
                                           tGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTgg                                                                                                         <  3:805055/149‑1 (MQ=255)
                                           tGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTgg                                                                                                         <  1:357186/149‑1 (MQ=255)
                                           tGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTATGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTgg                                                                                                         <  3:283348/149‑1 (MQ=255)
                                                      aaGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTaaa                                                                                              <  4:451982/149‑1 (MQ=255)
                                                      aaGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTaaa                                                                                              <  2:122788/149‑1 (MQ=255)
                                                          cGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACgtg                                                                                          <  3:772302/149‑1 (MQ=255)
                                                           gCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGGAACCCTGGAAGACCTGGGTCAGGCTAAACgtgt                                                                                         >  2:418259/1‑149 (MQ=255)
                                                                 gTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGAt                                                                                   >  1:715167/1‑149 (MQ=255)
                                                                  ttAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATc                                                                                  <  2:843077/149‑1 (MQ=255)
                                                                  ttAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATc                                                                                  <  3:202366/149‑1 (MQ=255)
                                                                    aaaGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATcaa                                                                                <  1:420330/149‑1 (MQ=255)
                                                                      aGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATcaaca                                                                              >  3:685523/1‑149 (MQ=255)
                                                                               cTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGAcacca                                                                     <  1:323975/149‑1 (MQ=255)
                                                                                      gATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTat                                                              >  4:719800/1‑149 (MQ=255)
                                                                                       aTCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTatc                                                             <  3:877336/149‑1 (MQ=255)
                                                                                       aTCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATATCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTatc                                                             <  2:45289/149‑1 (MQ=255)
                                                                                        tcgtcgTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTatca                                                            >  2:867232/1‑149 (MQ=255)
                                                                                        tcgtcgTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTatca                                                            >  1:740131/1‑149 (MQ=255)
                                                                                          gtcgtAAAGCAGCGGTGCAGGATATCGCAACGCTTCCTGGCCGTACCGTGATCGCTGAAGAGATCGTTATGGAGCTGGAAAAATCATCAGTGGACGACCTGGGTCAGGCTAAACGTGTTTTGATGTACGAAGGCAGCAGCACTatcatc                                                          <  2:265299/149‑1 (MQ=255)
                                                                                             gtAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGAt                                                       >  1:242105/1‑149 (MQ=255)
                                                                                             gtAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGAt                                                       >  1:37557/1‑149 (MQ=255)
                                                                                               aaaGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATgg                                                     >  4:190997/1‑149 (MQ=255)
                                                                                               aaaGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATgg                                                     >  1:439476/1‑149 (MQ=255)
                                                                                                  gCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGt                                                  >  3:229002/1‑149 (MQ=255)
                                                                                                    tATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTgg                                                <  3:415030/149‑1 (MQ=255)
                                                                                                    tATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTgg                                                >  2:227084/1‑149 (MQ=255)
                                                                                                    tATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTgg                                                >  1:286815/1‑149 (MQ=255)
                                                                                                    tATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTgg                                                >  3:181945/1‑149 (MQ=255)
                                                                                                     aTGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTggg                                               >  1:32742/1‑149 (MQ=255)
                                                                                                         tgcAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTggg                                               <  4:475979/145‑1 (MQ=255)
                                                                                                         tgcAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTggg                                               >  3:475979/1‑145 (MQ=255)
                                                                                                         tgcAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTgaa                                           >  1:28546/1‑149 (MQ=255)
                                                                                                         tgcAGGATATCGCAACCATGCCTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTgaa                                           >  2:83806/1‑149 (MQ=255)
                                                                                                         tacAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTgaa                                           >  3:676200/3‑149 (MQ=255)
                                                                                                           cAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATcaac                                                                               >  3:507198/1‑111 (MQ=255)
                                                                                                               atatCGCAAACCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCt                                     <  2:131524/149‑1 (MQ=255)
                                                                                                                 atCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGc                                   >  2:578341/1‑149 (MQ=255)
                                                                                                                   cGCAACCCTGACTGGCGGTATCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCaa                                 >  1:713833/1‑149 (MQ=255)
                                                                                                                    gCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAAt                                >  4:184822/1‑149 (MQ=255)
                                                                                                                        cccTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAg                            <  2:355635/149‑1 (MQ=255)
                                                                                                                         ccTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGTAAAAAGCAACCCTGGAAGACGAGGGGCAGGCTAAAGTGGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAgg                           >  4:588680/1‑149 (MQ=255)
                                                                                                                               tGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgtg                     >  3:45311/1‑149 (MQ=255)
                                                                                                                               tGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgtg                     <  4:586486/149‑1 (MQ=255)
                                                                                                                                ggCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAATCTGCAATCCAGGGCCgtgt                    <  3:729451/149‑1 (MQ=255)
                                                                                                                                ggCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgtgt                    <  2:740131/149‑1 (MQ=255)
                                                                                                                                 gCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGtt                   <  4:181945/149‑1 (MQ=255)
                                                                                                                                      aCCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCa              >  1:668825/1‑149 (MQ=255)
                                                                                                                                       taccgATCTCTTCAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAg             >  3:838992/5‑149 (MQ=255)
                                                                                                                                       taccgATCTCTTCAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAg             >  1:404772/5‑149 (MQ=255)
                                                                                                                                       taccgATCTCTTCAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAg             >  2:356233/5‑149 (MQ=255)
                                                                                                                                         gTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGAt           <  2:32742/149‑1 (MQ=255)
                                                                                                                                         gTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGAt           >  4:202452/1‑149 (MQ=255)
                                                                                                                                          tGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATc          >  4:291969/1‑149 (MQ=255)
                                                                                                                                           gATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATcc         <  1:132272/149‑1 (MQ=255)
                                                                                                                                            aTCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCg        <  2:892975/149‑1 (MQ=255)
                                                                                                                                                 tGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTcagc   >  2:232141/1‑149 (MQ=255)
                                                                                                                                                  gAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTcagca  >  2:167877/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGTCGTAAAGCTATGCTGCAGGATATCGCAACCCTGACTGGCGGTACCGTGATCTCTGAAGAGATCGGTATGGAGCTGGAAAAAGCAACCCTGGAAGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCA  >  NC_000913/4371785‑4372079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: