| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,387,774 | G→T | 5.1% | P533T (CCG→ACG) | mscM ← | mechanosensitive channel protein, miniconductance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,387,774 | 0 | G | T | 5.1% | 346.1 / 7.0 | 99 | P533T (CCG→ACG) | mscM | mechanosensitive channel protein, miniconductance |
| Reads supporting (aligned to +/- strand): ref base G (43/51); new base T (2/3); total (45/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01 | |||||||||||
CAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATC > NC_000913/4387628‑4387922 | cAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTa > 4:124448/1‑149 (MQ=255) aGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAAc > 1:782378/1‑149 (MQ=255) aGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAAc > 3:690617/1‑149 (MQ=255) gCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACg > 2:388137/1‑149 (MQ=255) gTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTg < 3:22828/149‑1 (MQ=255) tCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGAc < 4:713076/149‑1 (MQ=255) gggcgggcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGTTACCGGTgctgt < 3:471514/149‑5 (MQ=255) ggcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGcgacga < 3:71791/149‑1 (MQ=255) gcAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACgag > 2:670245/1‑149 (MQ=255) cAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACgaga > 3:106555/1‑149 (MQ=255) aaaGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGcgacga < 4:622229/146‑1 (MQ=255) aaaGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGcgacga > 3:622229/1‑146 (MQ=255) aaGGTGGCGCAAGTCAACACCCCCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGTCCGCCAGCGGATAAGGCCACGCCTCGCGCAAGACGTAACCCATCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAAt < 2:831267/149‑1 (MQ=255) aaGGTGGCGCAAATCATCACTACCCACAGCAGCGGTACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCCTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAAt < 3:602921/149‑1 (MQ=255) aaGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAAt < 2:848077/149‑1 (MQ=255) aaGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAAt < 1:388137/149‑1 (MQ=255) gTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGa < 4:44395/149‑1 (MQ=255) tGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGAc < 2:351258/149‑1 (MQ=255) tcatcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAaga < 3:544006/149‑1 (MQ=255) tcatcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAaga < 1:873919/149‑1 (MQ=255) tcatcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAaga < 3:324032/149‑1 (MQ=255) atcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGTTACCGGTgctgt < 4:411224/125‑5 (MQ=255) atcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGTTACCGGTgctgt > 3:411224/1‑121 (MQ=255) atcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGt < 2:782378/149‑1 (MQ=255) atcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGt > 2:880981/1‑149 (MQ=255) atcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGt > 3:731101/1‑149 (MQ=255) atcaCTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGt > 4:858716/1‑149 (MQ=255) tACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCa > 1:288305/1‑149 (MQ=255) ccACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACg > 3:102433/1‑149 (MQ=255) ccACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACg < 3:166619/149‑1 (MQ=255) cagcagCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCa < 2:216861/149‑1 (MQ=255) ggCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGaa < 4:106555/149‑1 (MQ=255) gCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAg > 3:288936/1‑149 (MQ=255) aCGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTg > 4:42125/1‑149 (MQ=255) cGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa < 1:700767/149‑1 (MQ=255) tGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATcc < 1:328441/149‑1 (MQ=255) ggCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCt < 4:827842/149‑1 (MQ=255) ggCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCt < 4:258882/149‑1 (MQ=255) gCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTg > 4:226759/1‑149 (MQ=255) ccGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 2:31703/149‑1 (MQ=255) ccGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 4:690617/149‑1 (MQ=255) ccGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa > 2:737080/1‑149 (MQ=255) ccGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 1:66798/149‑1 (MQ=255) ccGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 3:456322/149‑1 (MQ=255) ccGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 4:282189/149‑1 (MQ=255) acacCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCAc < 2:406444/149‑1 (MQ=255) acCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACtt < 2:42120/149‑1 (MQ=255) cATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTg > 3:793764/1‑149 (MQ=255) aTCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCTCAACGTCAGCCAGAAGTGATACTGAGTCACTTTGc > 1:173397/1‑149 (MQ=255) aTCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa > 4:701039/1‑139 (MQ=255) aTCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 3:701039/139‑1 (MQ=255) aTCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGc > 2:795138/1‑149 (MQ=255) ccAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACTCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAAc < 3:553224/149‑1 (MQ=255) ccAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAAc > 2:527221/1‑149 (MQ=255) aTCGCGACCGCCAGCGGATAAGGCCACGACTCGCGCAAGCCGTGACCCAGCGTCATCCACAGCACCGGTGACGGTGATGCGACGAGAATCGACCAGACAAGAGTGCGCAACGTCAGCCAGAAGCGATCCTGAGTCACTTTGCCAACttt < 4:548698/149‑1 (MQ=255) tCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTg > 4:422052/1‑104 (MQ=255) tCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTg < 3:422052/104‑1 (MQ=255) tCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTc < 4:753286/149‑1 (MQ=255) tCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTc < 2:838406/149‑1 (MQ=255) cgcgACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCTTCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTcg > 3:76888/1‑149 (MQ=255) cgACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTcgcc > 3:303731/1‑149 (MQ=255) cgACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTcgcc < 4:731101/149‑1 (MQ=255) aTAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCaa > 3:810036/1‑149 (MQ=255) ggCCACGCCTCGCGC‑AGCCGTAACCCAGCGTCATCCACAGCACCGGTAACg > 2:211582/1‑51 (MQ=255) ggCCACGCCTCGCGC‑AGCCGTAACCCAGCGTCATCCACAGCACCGGTAACg < 1:211582/51‑1 (MQ=255) gCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa > 3:501316/1‑110 (MQ=255) gCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 4:501316/110‑1 (MQ=255) ccACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAAc > 1:161792/1‑149 (MQ=255) ccACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAAc < 2:813624/149‑1 (MQ=255) ccACGCCTCGCGCAAGCCGTAACCCAGCGACAGCCACAGCAACGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTACGTCCCTTTGCCAACTTTCGCGGCCGAACGTTCAACAGAAc > 2:63264/1‑149 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa < 2:466864/97‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGa > 1:466864/1‑97 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGAt < 2:91563/98‑1 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGAt > 1:91563/1‑98 (MQ=255) cTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTAACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGa > 2:760603/1‑149 (MQ=255) tCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGaa > 2:688121/1‑149 (MQ=255) tCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGaa > 1:757843/1‑149 (MQ=255) cAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAgc < 2:496330/149‑1 (MQ=255) cAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAgc < 2:281741/149‑1 (MQ=255) aGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAgcgg < 2:846320/149‑1 (MQ=255) gCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAgcggc < 2:23558/149‑1 (MQ=255) gTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa > 2:696992/1‑91 (MQ=255) gTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 1:696992/91‑1 (MQ=255) gTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCAGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGcgcg > 4:607697/1‑149 (MQ=255) ccAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTaa > 2:161977/1‑149 (MQ=255) cAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTaaa < 1:561643/149‑1 (MQ=255) cAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTaaa > 2:561643/1‑149 (MQ=255) aGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa < 3:622711/84‑1 (MQ=255) aGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGa > 4:622711/1‑84 (MQ=255) aGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAAt > 2:845754/1‑149 (MQ=255) aGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAAt < 2:866325/149‑1 (MQ=255) gCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATa < 3:222751/149‑1 (MQ=255) gTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACt > 1:41693/1‑149 (MQ=255) gTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACt < 1:29937/149‑1 (MQ=255) tCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCaa > 2:370875/1‑149 (MQ=255) cgtcACCGTTACCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGt > 3:771621/4‑134 (MQ=255) cgtcACCGTTACCGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGt < 4:771621/131‑1 (MQ=255) ccGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGa > 1:363856/1‑149 (MQ=255) ccGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGa < 2:402670/149‑1 (MQ=255) gTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATc > 3:104196/1‑149 (MQ=255) | CAAGCCGTTCGGGCGGGCAAAGGTGGCGCAAATCATCACTACCCACAGCAGCGGCACGGTGGCCGTTACACCATCGCCAATCGCGACCGCCAGCGGATAAGGCCACGCCTCGCGCAAGCCGTAACCCAGCGTCATCCACAGCACCGGTAACGGTGACGCGACGAGAATCGACCAGAAAAGAGTGCGCAACGTCAGCCAGAAGTGATCCTGAGTCACTTTGCCAACTTTCGCCGCCGAACGTTCAAGAAAACGGGTGAAATAGCGGCGCGAGTAAATACTGCAACCGACCAGAATC > NC_000913/4387628‑4387922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |