Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,408,759 G→A 6.4% L702L (CTG→CTA) ‡ rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,408,7590GA 6.4% 402.1 / 12.4 124L702L (CTG→CTA) ‡rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base G (67/49);  new base A (2/6);  total (69/55)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

Only 100 of 126 total aligned reads displayed.

CGTCACTGGCTTTGGCTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCG  >  NC_000913/4408612‑4408907
                                                                                                                                                   |                                                                                                                                                    
cGTCACTGGCTTTGGCTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTgg                                                                                                                                                     <  1:377484/149‑1 (MQ=255)
  tCACTGGCTTTGGCTTCTTCGTCCGTCTGGAGGACTTGTTCATGGATGGTCTGGTCCGTGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGTTAGGGCATCGCCTGAGGCGGGAATCGAGCTGCCAGACTTATCGCCTGGGc                                                                                                                                                   >  4:82046/1‑149 (MQ=255)
    aCTGGCTTTGGCTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGa                                                                                                                                                 <  2:796449/149‑1 (MQ=255)
       ggctttggcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCCAGGCGAtaa                                                                                                                                              <  1:122467/149‑3 (MQ=255)
          tttggcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCCAGGCGAtaagtc                                                                                                                                           <  3:248149/149‑6 (MQ=255)
           ttggcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCCAGGCGAtaagtct                                                                                                                                          <  3:598851/149‑7 (MQ=255)
              gcttCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGaa                                                                                                                                       >  4:795662/1‑149 (MQ=255)
                ttcttcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGt                                                                                                                                     >  1:345398/1‑149 (MQ=255)
                ttcttcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGt                                                                                                                                     >  3:781490/1‑149 (MQ=255)
                ttcttcGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCCGATGGGGGAATCCAGCGGCCAGACTTATCGCCCAGGCGAtaa                                                                                                                                              >  1:820685/1‑138 (MQ=255)
                    tcGTCCGTCTGGACGACTTGTTCATTGATGGTCTAGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTcgc                                                                                                                                 <  3:528286/149‑1 (MQ=255)
                      gtccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGt                                                                                                                               >  1:843292/1‑149 (MQ=255)
                       tccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCAGGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTc                                                                                                                              >  1:734768/1‑149 (MQ=255)
                        ccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCCAGGCGAtaa                                                                                                                                              <  3:636330/132‑3 (MQ=255)
                        ccgtcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCCAGGCGAtaa                                                                                                                                              >  4:636330/1‑130 (MQ=255)
                           tcTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAg                                                                                                                          >  4:536924/1‑149 (MQ=255)
                            cTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGc                                                                                                                         <  1:519057/149‑1 (MQ=255)
                                  gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTaa                                                                                                                   >  3:647603/1‑149 (MQ=255)
                                  gacTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTaa                                                                                                                   >  3:345100/1‑149 (MQ=255)
                                     ttGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGAt                                                                                                                                                >  4:157526/1‑117 (MQ=255)
                                     ttGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGAt                                                                                                                                                <  3:157526/117‑1 (MQ=255)
                                     ttGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAatat                                                                                                                >  4:53661/1‑149 (MQ=255)
                                      tGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATg                                                                                                               <  1:746672/149‑1 (MQ=255)
                                       gTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATgg                                                                                                              <  2:764018/149‑1 (MQ=255)
                                           aTTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa                                                                                                          >  3:255737/1‑149 (MQ=255)
                                           aTTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa                                                                                                          >  1:497915/1‑149 (MQ=255)
                                           aTTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa                                                                                                          >  2:880382/1‑149 (MQ=255)
                                           aTTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa                                                                                                          <  1:405419/149‑1 (MQ=255)
                                           aTTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa                                                                                                          >  4:263568/1‑149 (MQ=255)
                                           aTTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGa                                                                                                          >  2:405419/1‑149 (MQ=255)
                                            ttGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAg                                                                                                         >  1:270946/1‑149 (MQ=255)
                                            ttGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAg                                                                                                         >  3:865841/1‑149 (MQ=255)
                                             tGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgc                                                                                                        <  2:734768/149‑1 (MQ=255)
                                             tGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTAGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgc                                                                                                        <  3:619328/149‑1 (MQ=255)
                                                 ggtctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCaa                                                                                                    >  2:469438/1‑149 (MQ=255)
                                                   tctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCaaaa                                                                                                  <  1:858323/149‑1 (MQ=255)
                                                    ctggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAAt                                                                                                 <  2:863699/149‑1 (MQ=255)
                                                     tggtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATc                                                                                                <  1:898025/149‑1 (MQ=255)
                                                       gtcCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGa                                                                                              >  2:164127/1‑149 (MQ=255)
                                                          cATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACtt                                                                                           <  2:270946/149‑1 (MQ=255)
                                                           aTGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTg                                                                                                                                          >  4:458155/1‑101 (MQ=255)
                                                           aTGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACttt                                                                                          >  2:295402/1‑149 (MQ=255)
                                                            tGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTa                                                                                         >  4:392907/1‑149 (MQ=255)
                                                             gTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAg                                                                                        >  4:459421/1‑149 (MQ=255)
                                                              tctcTTCGCTGGACGATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGGTGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGc                                                                                       >  3:879889/1‑149 (MQ=255)
                                                              tctcTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgcg                                                                                                       <  2:85153/133‑1 (MQ=255)
                                                              tctcTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAgcg                                                                                                       >  1:85153/1‑133 (MQ=255)
                                                              tctcTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGc                                                                                       >  4:66765/1‑149 (MQ=255)
                                                              tctcTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGc                                                                                       <  1:612891/149‑1 (MQ=255)
                                                                tctTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCt                                                                                     >  1:69551/1‑149 (MQ=255)
                                                                  ttCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGa                                                                                   >  4:576287/1‑149 (MQ=255)
                                                                     gCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGAtct                                                                                >  4:50370/1‑149 (MQ=255)
                                                                     gCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGAtct                                                                                >  1:354297/1‑149 (MQ=255)
                                                                              tGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAAc                                                                       <  1:535020/149‑1 (MQ=255)
                                                                               gACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAAcg                                                                      >  3:378756/1‑149 (MQ=255)
                                                                                actactATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAAcgc                                                                     >  1:821898/1‑149 (MQ=255)
                                                                                actactATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAAcgc                                                                     >  3:422477/1‑149 (MQ=255)
                                                                                 ctactaTCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGAc                                                                                             <  4:385933/124‑1 (MQ=255)
                                                                                 ctactaTCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGAc                                                                                             >  3:385933/1‑124 (MQ=255)
                                                                                  tactaTCGCTTTGACCAGGTGGGGCAACGCCTGATGGGGGAATCCAGCGACCAGACTTATCGCCTGGGCGATGGCGTGGAAGTTCGCGGCGAAGCGGATAATATGGACGAGCGCAAAGTCGACTTTAGCCTGATCTCCAGCGAACgcgc                                                                   >  2:330355/1‑149 (MQ=255)
                                                                                  tactaTCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACgcgc                                                                   >  3:472055/1‑149 (MQ=255)
                                                                                    ctaTCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCAc                                                                 <  4:341143/149‑1 (MQ=255)
                                                                                     taTCGCTTTGACTAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCTCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATTTCCAGCGAACCCGCAcc                                                                >  4:745339/1‑149 (MQ=255)
                                                                                       tCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACcgc                                                              >  2:843001/1‑149 (MQ=255)
                                                                                       tCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACcgc                                                              <  3:665377/149‑1 (MQ=255)
                                                                                        cGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACcgcg                                                             <  1:81780/149‑1 (MQ=255)
                                                                                         gCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCgcgc                                                            >  3:578164/1‑149 (MQ=255)
                                                                                          cTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCa                                                           >  2:725717/1‑149 (MQ=255)
                                                                                           tttGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCaa                                                          >  1:89885/1‑149 (MQ=255)
                                                                                           tttGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGCCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCaa                                                          >  4:600283/1‑149 (MQ=255)
                                                                                                ccAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCg                                                     <  1:864531/149‑1 (MQ=255)
                                                                                                 cAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCgg                                                    <  1:553823/149‑1 (MQ=255)
                                                                                                 cAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCgg                                                    >  4:208330/1‑149 (MQ=255)
                                                                                                 cAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCgg                                                    <  3:392907/149‑1 (MQ=255)
                                                                                                  aGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGt                                                   >  3:856622/1‑149 (MQ=255)
                                                                                                  aGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGt                                                   <  2:150424/149‑1 (MQ=255)
                                                                                                         gCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACgg                                            >  1:855156/1‑149 (MQ=255)
                                                                                                           aaCGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGgcg                                          >  1:224049/1‑149 (MQ=255)
                                                                                                               ccTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGcgcgcg                                      >  2:559086/1‑149 (MQ=255)
                                                                                                                cTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCga                                     >  4:359663/1‑149 (MQ=255)
                                                                                                                 tGATGGGGGAATCCAGCGGACAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCCCGTCGAAGCGGCTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCgag                                    >  4:610925/1‑149 (MQ=255)
                                                                                                                  gATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCgaga                                   >  2:187336/1‑149 (MQ=255)
                                                                                                                    tGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGaaa                                 >  3:566625/1‑149 (MQ=255)
                                                                                                                    tGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGaaa                                 >  4:804562/1‑149 (MQ=255)
                                                                                                                    tGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCCTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGaaa                                 <  2:280552/149‑1 (MQ=255)
                                                                                                                      ggggAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGc                               <  4:494683/149‑1 (MQ=255)
                                                                                                                       gggAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCg                              <  4:353011/149‑1 (MQ=255)
                                                                                                                       gggAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCg                              >  2:12911/1‑149 (MQ=255)
                                                                                                                             ccAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGaaaaaa                        <  4:647603/149‑1 (MQ=255)
                                                                                                                              cAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAg                       <  2:497915/149‑1 (MQ=255)
                                                                                                                                gCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGc                     <  1:164127/149‑1 (MQ=255)
                                                                                                                                    ccAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATg                 <  3:114630/149‑1 (MQ=255)
                                                                                                                                      aGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCa               >  1:9906/1‑149 (MQ=255)
                                                                                                                                       taCTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCGTGATCTCCAGCGAAGGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAg              >  4:764780/2‑149 (MQ=255)
                                                                                                                                       gACTTATCGCCTGGGCGATCGCGTGGCAGTTCGCGTCGAAGCGGTTAAAAAGGACGAGCGCAAAAGCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAg              <  2:843944/149‑1 (MQ=255)
                                                                                                                                          ttATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTa           >  2:886521/1‑149 (MQ=255)
                                                                                                                                                ccTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAg     <  3:466695/149‑1 (MQ=255)
                                                                                                                                                 cTGGGCGATCGCGTGGAAGGTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACTTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAgg    <  4:211229/149‑1 (MQ=255)
                                                                                                                                                  tGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAggc   >  3:469709/1‑149 (MQ=255)
                                                                                                                                                  tGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAggc   <  3:536924/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTCACTGGCTTTGGCTTCTTCGTCCGTCTGGACGACTTGTTCATTGATGGTCTGGTCCATGTCTCTTCGCTGGACAATGACTACTATCGCTTTGACCAGGTAGGGCAACGCCTGATGGGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCG  >  NC_000913/4408612‑4408907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: