Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,409,168 A→C 5.0% intergenic (+73/‑107) rnr → / → rlmB exoribonuclease R, RNase R/23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,409,1680AC 5.0% 280.3 / 6.3 80intergenic (+73/‑107)rnr/rlmBexoribonuclease R, RNase R/23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (34/42);  new base C (2/2);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

CCATCGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACG  >  NC_000913/4409021‑4409302
                                                                                                                                                   |                                                                                                                                      
ccATCGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                       <  3:70680/149‑1 (MQ=255)
 cATCGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                      >  3:280696/1‑149 (MQ=255)
 cATCGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                      >  1:296237/1‑149 (MQ=255)
 cATCGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                      <  4:657180/149‑1 (MQ=255)
   tCGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                    <  3:774618/149‑1 (MQ=255)
    cGGCGAAAACGCAGAAAATAGCTGCAGCGAGCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCACGCCGCATCCGGCATGAAcaaa                                                                                                                                   <  1:187336/149‑4 (MQ=255)
    cGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCACGCCGCATCCGGCATGAAcaaa                                                                                                                                   <  4:206863/149‑4 (MQ=255)
    cGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGAAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCACGCCGCATCCGGCATGAAcaaa                                                                                                                                   <  1:498146/149‑4 (MQ=255)
      gCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                                                                                 >  3:803826/1‑149 (MQ=255)
       cGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                <  2:280681/149‑1 (MQ=255)
        gAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                               >  1:621502/1‑149 (MQ=255)
            aCGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                           <  1:330355/149‑1 (MQ=255)
             cGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                          <  1:725717/149‑1 (MQ=39)
              gCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                         <  2:202842/149‑1 (MQ=255)
              gCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATAAGTCGTTCACGCCGCATCCGGca                                                                                                                         <  2:69534/149‑2 (MQ=255)
               cAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                                                        <  4:436335/149‑1 (MQ=255)
                       aGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGGGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATACTc                                                                                                                <  4:176460/149‑1 (MQ=255)
                         cTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCAc                                                                                                              <  1:341393/149‑1 (MQ=255)
                         cTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCAc                                                                                                              <  4:803826/149‑1 (MQ=255)
                              gCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCaaa                                                                                                         <  2:282383/149‑1 (MQ=255)
                                gACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAAtt                                                                                                       <  1:718507/149‑1 (MQ=255)
                                   cAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                      <  1:337941/115‑1 (MQ=255)
                                   cAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                      >  2:337941/1‑115 (MQ=255)
                                   cAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                                                                    >  2:603760/1‑149 (MQ=255)
                                   cAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTCCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                                                                    <  1:782328/149‑1 (MQ=255)
                                     aagcgaagcgTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAata                                                                                                  >  3:445906/1‑149 (MQ=255)
                                          aagcgTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAACAGGTTTAGATTGCTGACAAAATGCGCTTTGTTCCTGCCGGATGCGGTGTGACCGTCTTATCCGGCCTACATAATCACGCAAATGCAATATATTg                                                                                             >  2:208856/1‑149 (MQ=255)
                                              gTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCACTATATTGCaga                                                                                         <  3:764780/149‑1 (MQ=255)
                                              gTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCaga                                                                                         <  4:638043/149‑1 (MQ=255)
                                                 cggcgAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGAt                                                                                      <  4:364746/149‑1 (MQ=255)
                                                     gaagaaAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATg                                                                                  <  3:804562/149‑1 (MQ=255)
                                                         aaaaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAgg                                                                              >  1:493665/1‑149 (MQ=255)
                                                           aaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                      >  4:851757/1‑91 (MQ=255)
                                                           aaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                      <  3:851757/91‑1 (MQ=255)
                                                           aaaaGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGcc                                                                            >  2:172811/1‑149 (MQ=255)
                                                                    agagTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACg                                                                   >  3:441322/1‑149 (MQ=255)
                                                                     gagTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                                                      <  1:79831/97‑1 (MQ=255)
                                                                     gagTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                                                      >  2:79831/1‑97 (MQ=255)
                                                                     gagTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGt                                                                  >  3:725762/1‑149 (MQ=255)
                                                                      agTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTa                                                                 <  1:886521/149‑1 (MQ=255)
                                                                          aTCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGc                                                             <  3:740872/149‑1 (MQ=255)
                                                                          aTCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGc                                                             >  4:772275/1‑149 (MQ=255)
                                                                             aaTACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATc                                                          <  2:296237/149‑1 (MQ=255)
                                                                                aCCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAgg                                                       >  4:262215/1‑149 (MQ=255)
                                                                                  ccTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCa                                                     >  4:587502/1‑149 (MQ=255)
                                                                                   ctctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCaa                                                    >  2:869663/1‑149 (MQ=255)
                                                                                    tctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCaaa                                                                                                         <  2:705723/95‑1 (MQ=255)
                                                                                    tctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCaaa                                                                                                         >  1:705723/1‑95 (MQ=255)
                                                                                      tttAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCTTCAGGCAAAAc                                                 >  3:572966/1‑149 (MQ=255)
                                                                                           aagaagAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGc                                                             >  1:895895/1‑132 (MQ=255)
                                                                                           aagaagAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGc                                                             <  2:895895/132‑1 (MQ=255)
                                                                                            agaagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAaac                                           <  2:800636/149‑1 (MQ=255)
                                                                                               agagGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAaacaac                                        >  2:712506/1‑149 (MQ=255)
                                                                                                 agGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGa                                      <  3:234871/149‑1 (MQ=255)
                                                                                                 agGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGa                                      <  3:262215/149‑1 (MQ=255)
                                                                                                  gggTTAGATTGCTGACAAAGTGCGCTTTGATCATGCCGGATGCTGCGTGAACGGCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCACAGACCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAg                                     <  1:208856/149‑1 (MQ=21)
                                                                                                   ggTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                          >  2:626867/1‑63 (MQ=255)
                                                                                                   ggTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                          <  1:626867/63‑1 (MQ=255)
                                                                                                   ggTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGa                                      >  2:449598/1‑147 (MQ=255)
                                                                                                   ggTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGa                                      <  1:449598/147‑1 (MQ=255)
                                                                                                    gTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTa                                   >  1:266087/1‑149 (MQ=255)
                                                                                                    gTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTa                                   >  3:672150/1‑149 (MQ=255)
                                                                                                      tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACa                                 >  2:435689/1‑149 (MQ=255)
                                                                                                             cTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATGATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGa                          <  1:869663/149‑1 (MQ=255)
                                                                                                             cTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGa                          >  2:702803/1‑149 (MQ=255)
                                                                                                               gACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTa                                                                 >  1:828676/1‑108 (MQ=255)
                                                                                                               gACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTa                                                                 <  2:828676/108‑1 (MQ=255)
                                                                                                               gACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGc                        >  4:109259/1‑149 (MQ=255)
                                                                                                               gACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGc                        <  3:531285/149‑1 (MQ=255)
                                                                                                                aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                                                                    >  1:171997/1‑72 (MQ=255)
                                                                                                                aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                                                                    <  2:171997/72‑1 (MQ=255)
                                                                                                                aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAg                         <  2:414283/147‑1 (MQ=255)
                                                                                                                aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAg                         >  1:414283/1‑147 (MQ=255)
                                                                                                                     aTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATg                  >  2:600375/1‑149 (MQ=255)
                                                                                                                           tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATAAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTAc            >  1:598777/1‑149 (MQ=255)
                                                                                                                            ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACg           <  2:266087/149‑1 (MQ=255)
                                                                                                                            ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACg           <  1:12911/149‑1 (MQ=255)
                                                                                                                             tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACgg          <  2:24000/149‑1 (MQ=255)
                                                                                                                             tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACgg          <  1:295979/149‑1 (MQ=255)
                                                                                                                                  aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAg                                                        >  3:442605/3‑98 (MQ=18)
                                                                                                                                     ccGGATAAGGCGTTCACGCCGCATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACg  >  1:876044/1‑149 (MQ=37)
                                                                                                                                                   |                                                                                                                                      
CCATCGGCGAAAACGCAGAAAATAGCTGCAGCGACCAAAGCGAAGCGTGCGGCGAAGAAAAAAGTGGCAGAGTGATCAATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACG  >  NC_000913/4409021‑4409302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: