Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,694 A→G 9.3% intergenic (+31/+29) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,6940AG 9.3% 198.1 / 8.4 65intergenic (+31/+29)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base G (3/3);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

TGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTC  >  NC_000913/681546‑681839
                                                                                                                                                    |                                                                                                                                                 
tGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCa                                                                                                                                                   >  4:72798/1‑149 (MQ=255)
tGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCa                                                                                                                                                   >  1:554257/1‑149 (MQ=255)
   agaaAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCg                                                                                                                                                >  2:83789/1‑149 (MQ=255)
    gaaAAAAATGATGAGGGTAAAAAACCACAGAGCACTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGt                                                                                                                                               <  3:797485/149‑1 (MQ=255)
         aaaTGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGGGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAcg                                                                                                                                          >  4:588631/1‑149 (MQ=255)
                  gggTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCg                                                                                                                                 <  2:545675/149‑1 (MQ=255)
                     tGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCa                                                                                                                              >  3:442181/1‑149 (MQ=255)
                        aaaaCCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                                                           >  3:534514/1‑149 (MQ=255)
                          aaCCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgt                                                                                                                         <  1:540684/149‑1 (MQ=255)
                             cacaGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGtt                                                                                                                      >  2:585820/1‑149 (MQ=255)
                                  agCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATTGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTAtcg                                                                                                                 <  3:563272/149‑3 (MQ=255)
                                  agCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATgg                                                                                                                 <  4:534514/149‑1 (MQ=255)
                                  agCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGGGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATgg                                                                                                                 <  3:588631/149‑1 (MQ=255)
                                   gCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                                                                <  1:546228/149‑1 (MQ=255)
                                     aTTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAt                                                                                                              <  4:60208/149‑1 (MQ=255)
                                           aGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                                                        <  2:375333/149‑1 (MQ=255)
                                                 aTCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaa                                                                                                  >  1:871599/1‑149 (MQ=255)
                                                  tCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaaa                                                                                                 >  1:728878/1‑149 (MQ=255)
                                                    aaaaTCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaata                                                                                               >  4:842325/1‑149 (MQ=255)
                                                        tCAGTTTAACGGTGCTGGTGATAGGCTATGCACTCGGCAAAATCGCGATGTTGTTTAGCTGAAGATGCGTTTTTTGCGGCAATGCCTGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTACATAAtcg                                                                                           >  2:682333/1‑149 (MQ=255)
                                                          aGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgct                                                                                         >  1:611704/1‑149 (MQ=255)
                                                                          tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCaa                                                                                                         <  4:400432/117‑1 (MQ=255)
                                                                          tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCaa                                                                                                         >  3:400432/1‑117 (MQ=255)
                                                                            aTAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTc                                                                       >  3:295302/1‑149 (MQ=255)
                                                                                      ctctCGGCAAAATCGCGATGTTGTTTAGCCGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCgg                                                             >  4:652451/1‑149 (MQ=255)
                                                                                       tctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                                                            >  1:492802/1‑149 (MQ=255)
                                                                                       tctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGGTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCggg                                                            >  1:524099/1‑149 (MQ=255)
                                                                                         tcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGcc                                                          <  4:781102/149‑1 (MQ=255)
                                                                                           ggCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                                                        >  4:27144/1‑149 (MQ=255)
                                                                                            gCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCata                                                       >  4:527547/1‑149 (MQ=255)
                                                                                             cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                                                      <  3:202416/149‑1 (MQ=255)
                                                                                             cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCatat                                                      <  2:827167/149‑1 (MQ=255)
                                                                                              aaaaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATc                                                     >  1:301198/1‑149 (MQ=255)
                                                                                                  tCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt                                                 <  1:361974/149‑1 (MQ=255)
                                                                                                  tCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt                                                 <  2:552877/149‑1 (MQ=255)
                                                                                                    gcgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGa                                               <  1:162956/149‑1 (MQ=255)
                                                                                                     cgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc                                              <  4:361517/149‑1 (MQ=255)
                                                                                                     cgATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc                                              <  3:336463/149‑1 (MQ=255)
                                                                                                       aTGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTg                                            >  2:424388/1‑149 (MQ=255)
                                                                                                         gttgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCa                                          >  3:601859/1‑149 (MQ=255)
                                                                                                           tgttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAc                                        <  2:728878/149‑1 (MQ=255)
                                                                                                            gttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcg                                       >  1:767696/1‑149 (MQ=255)
                                                                                                            gttTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcg                                       >  2:651567/1‑149 (MQ=255)
                                                                                                                aGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcgccgc                                   <  2:611704/149‑1 (MQ=255)
                                                                                                                 gCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCa                                  <  3:34800/149‑1 (MQ=255)
                                                                                                                       gacgtgtTTTCTGTGGCGATGCAGGATACCGCGTTTACGCCGCATCCGGCAGCCGTTTATATTGGCTCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCCACGCCGCACCaaaa                            <  4:588453/146‑1 (MQ=255)
                                                                                                                         tgtgTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATc                          >  4:628428/1‑149 (MQ=255)
                                                                                                                           tgtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGGCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCgg                        <  2:47464/149‑1 (MQ=255)
                                                                                                                            gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGt                       <  3:74792/149‑1 (MQ=255)
                                                                                                                              tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGa                     <  1:180306/149‑1 (MQ=255)
                                                                                                                              tttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGa                     <  1:83789/149‑1 (MQ=255)
                                                                                                                               ttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGAt                    <  1:585820/149‑1 (MQ=255)
                                                                                                                                 ttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCa                  <  1:709452/149‑1 (MQ=255)
                                                                                                                                  tGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCAc                 <  3:467596/149‑1 (MQ=255)
                                                                                                                                    cgcCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCAccc               <  1:118166/149‑1 (MQ=255)
                                                                                                                                      ccGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCgg             >  3:262617/1‑149 (MQ=255)
                                                                                                                                        gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGcc           >  1:544182/1‑149 (MQ=255)
                                                                                                                                         aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCg          <  4:456458/149‑1 (MQ=255)
                                                                                                                                            ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtc       <  1:140443/149‑1 (MQ=255)
                                                                                                                                             ctttcgtaGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtct      <  1:781858/142‑1 (MQ=255)
                                                                                                                                             cGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtct      >  1:149559/1‑149 (MQ=255)
                                                                                                                                              ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                                                        <  2:302751/50‑1 (MQ=17)
                                                                                                                                              ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                                                        >  1:302751/1‑50 (MQ=14)
                                                                                                                                              ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtctc     >  3:254829/1‑149 (MQ=255)
                                                                                                                                              ggATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtctc     <  2:301198/149‑1 (MQ=255)
                                                                                                                                                aTGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt                                                 >  4:320113/1‑103 (MQ=39)
                                                                                                                                                aTGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGttt                                                 <  3:320113/103‑1 (MQ=39)
                                                                                                                                                 tGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCtcttc  <  1:853536/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTC  >  NC_000913/681546‑681839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: