Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,116,705 T→G K173T (AAA→ACA)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,116,7050TG97.3% 791.2 / NA 227K173T (AAA→ACA) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (6/0);  new base G (115/104);  total (121/106)
Fisher's exact test for biased strand distribution p-value = 3.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 227 total aligned reads displayed.

GTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTG  >  NC_000913/4116431‑4116976
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                
        ccggccgttcttTGACGAAGAACCGACCAACAAGCGCGGGCGACTGGTAACCGGAGATACCGGGAAGTGAAAAACGCGGGCCGCATTTGCGCCCAACTGTGTGCTGACCGTCTACTTCGGAAGAACGACTCAGTTCTGGCAGCATCTCACGCGGCATATCCAGGACTTCCACCATTTTGTCGTCCCGGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCTTGAGTCATTGTCCAGAt                                                                                                                                                                                                                                                                           <  3:633756/267‑1 (MQ=255)
        ccATCCCGTCTTTCACGCACAACTGACCAGACAGCGCTGCCTGCTTGTCACCGGCGATCCCGGATATTTGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCa                                                                                                                                                                                                                                                                     <  2:1026960/280‑1 (MQ=255)
           accgttcgtTCAGACACCACTGACCAAACAGGGGCTCGTGTTAGTCACCGGCGATCCCGGAGATTGAAAGACGCGTTCCGCCTTTGCCGCCAATGTTATGCTGACCGAATACTTCGGAAGAACGACGCACTTCTGGCATCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAAGCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGc                                                                                                                                                                                                                                                                       <  3:985549/271‑1 (MQ=255)
            cccTTCCTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGc                                                                                                                                                                                                                                                                       <  1:13035/274‑1 (MQ=255)
             ccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGc                                                                                                                                                                                                                                                                       <  1:164791/273‑1 (MQ=255)
                tcttTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGCTAAGACACGt                                                                                                                                                                                                                                                                  >  1:1230574/1‑275 (MQ=255)
                       cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCTTTTGCCGCCAATGTTAGTCTGACCGTATCCTTCGGAAGAACGACGCCCTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTCCCAGCATTTTGTAGTCCCAGTCCAGGGTATGGATTTCGAACAACATGGTACGAGAGGCGCTGGTGTAATCGGTCCAATGGGCAAGGCCCTGATATATTTGCCCAGTAAGCCACGTacacacc                                                                                                                                                                                                                                                           >  3:1374845/1‑269 (MQ=255)
                       cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATAGGTCACATGGACACGGCACTGAGTCATTTTCCAGCTAAGCACCTTATTAACCGTAcc                                                                                                                                                                                                                                                      >  2:194173/1‑280 (MQ=255)
                       cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGAACTTCCAGCATTTTGTCGTCCCAGTCACGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACCCGGCTCTGAGTCATTGCACAGATAACCAACGTATCAACTGTAcc                                                                                                                                                                                                                                                      >  4:397716/1‑280 (MQ=255)
                       cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCCCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCGGAGTCATTGTCCAGATAAGCCACGTATCAACCGTAcc                                                                                                                                                                                                                                                      >  2:719909/1‑280 (MQ=255)
                              cTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGACAGGCGTTGGTGTAATCGGTCACATGGCAACGGCACTGAGTCATTGTCCCGATAAGCCACGTATCACCCTTCCCa                                                                                                                                                                                                                                                     >  1:524152/1‑274 (MQ=255)
                                      aCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGTCGTTGGTGTCATCGGTCACATGGCCACGTCCCTGCGTCATTGTCTCGATACGCCACTGATCCACCGTTCCCCACAGCCATTcac                                                                                                                                                                                                                                        >  2:282373/1‑279 (MQ=255)
                                                                 tCCCGGAGATTTGAATCCGGTTGCCGCCTTTCCCGCCAATGTTAGTCTTACCGTATACTGCGGAAGAACGACGCACTTCTGGCAGCATTTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCgaga                                                                                                                                                                                                                  <  3:1230574/274‑1 (MQ=255)
                                                                   ccGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCca                                                                                                                                                                                                          <  2:218914/280‑1 (MQ=255)
                                                                     ggggtttGGAAGACGCGTACCGCCTGTGCCGCCAATGTTAGTCTGACCGGATACTTCGGAAGAACGACGCACTTCTGGCAGCATCGCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGAGGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcaca                                                                                                                                                                                                        <  4:878369/275‑1 (MQ=255)
                                                                     ggAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATGCACCACGACGTGCACGCTCGCGAGAGCCTTCcaca                                                                                                                                                                                                        >  2:337865/1‑280 (MQ=255)
                                                                      ggggttGGAAAACGCGTTCCGCCTTGGCCGCCAAGGTTAGGCTGCACGGATACTTCGGAAGAACGACGCACTTCTGGCAGCAGCTCGCGCGGAATATCCCGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcaca                                                                                                                                                                                                        <  4:1018858/275‑1 (MQ=255)
                                                                       agaTTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTc                                                                                                                                                                                                            >  1:992154/1‑274 (MQ=255)
                                                                           tGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCGACGTATCAACCGTACCAAACAGCAATTAACCACGACGTGCACGCTCGCGACAGGCTTCCACATGGt                                                                                                                                                                                                    >  2:190135/1‑278 (MQ=255)
                                                                              gaTACTCGTGCCGCCTTTGCCGCCAATGTTAGTCGAACCGTATACGTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGGCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACATATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGt                                                                                                                                                                                                    <  1:395453/274‑1 (MQ=255)
                                                                                   acgTCCCGCCGTTGCCGCCAAGGTTATTCGGACCGTATACTTCGGAAGAACGACGCAATTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATGTTGTCGTCCAAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAgg                                                                                                                                                                                               <  1:722667/274‑1 (MQ=255)
                                                                                                     cAATGTTATTCTGACCGTATACTTCGGAAGAACGCCGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGGGTCATTGTCCAGATAAGCCACGTATCAACCGTACTAAACAGCAATTCACCACGACGTTCACGCTCGCGAGAGCCTTCCACATGCCCGCAGACCCACTTCCATTTGGTGCCata                                                                                                                                                                        >  2:440454/1‑278 (MQ=255)
                                                                                                            tAGTCTGACCGTATACTTCGGAAGAACGACGAACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAGTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTa                                                                                                                                                                  <  2:397716/280‑1 (MQ=255)
                                                                                                             aGTCTGACGCGATAGTACGGAAGGACGACGCAGTTCTGGCAGAAGCTCGCGAGGAAGATCCAGCACTTACAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGAACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaa                                                                                                                                                                       <  3:1001840/274‑1 (MQ=255)
                                                                                                              gTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                                                                                                                                                                <  2:886910/280‑1 (MQ=255)
                                                                                                                   aCCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTTTAATCGGTCACATGGACACGGCCCTGAGCCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCCTCCCCATGGTCGAGGATCCACTTCACTTTCGTGCCAGAACAGTAc                                                                                                                                                                 >  1:1024655/1‑274 (MQ=255)
                                                                                                                   aCCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATTGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAACCCCCGTATCAACCGTACCAAACAGCCATTCACCACCACGTGAACCCTCGCGAGAGCCTCCCCCATGGTCGAGGCGCCACTTCCCTTTTGTGCCCTAAACGTAc                                                                                                                                                                 >  3:793517/1‑274 (MQ=255)
                                                                                                                   aCCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAACCAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCCCCTCACTTTGGTGCCAGAAACGTAc                                                                                                                                                                 >  3:957128/1‑274 (MQ=255)
                                                                                                                   aCCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCCGCAAAGTACGGGTc                                                                                                                                                            >  2:372541/1‑279 (MQ=255)
                                                                                                                   aCCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCAAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACAGAACCAAACAGCAATTCACCCCGACGTGCACGCTCGCGACACCCTTCCACATGGTTGAGGAACCACTTCCCTTTGCTGCCAGAACAgaagagggc                                                                                                                                                            >  4:920168/1‑271 (MQ=255)
                                                                                                                           cgtCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACGTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAg                                                                                                                                                   <  2:675788/278‑1 (MQ=255)
                                                                                                                            ttCGGACGAACCACGCACTTCTGGCAGCATGTCGCGCGGAAGATCCAGAACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACAGGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAGTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAg                                                                                                                                                   <  4:372541/279‑1 (MQ=255)
                                                                                                                             tCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATaa                                                                                                                                                                                                                                                                         >  1:883975/1‑160 (MQ=255)
                                                                                                                             tCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGCAAAGTACGGGTCAATc                                                                                                                                                        >  1:365319/1‑273 (MQ=255)
                                                                                                                             tCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAc                                                                                                                                                 <  2:579422/280‑1 (MQ=255)
                                                                                                                              cGGAAGAACGCCGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTCGGTGTAATAGGTCACATGGACACGGCCCTGAGTCATTGTCCAGTTAAGCAACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGTCCGTGTCTCCACTTCACATTGATGCTAAACAAGTACGGGCCAATcccc                                                                                                                                                     >  3:825308/1‑275 (MQ=255)
                                                                                                                              cGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAc                                                                                                                                                      <  3:899995/274‑1 (MQ=255)
                                                                                                                              cGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCAACACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCaa                                                                                                                                                   >  2:703215/1‑276 (MQ=255)
                                                                                                                              cGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTACAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGGCATGGTCCAGATAAGCAAGGTATAAACCGTACCAAAAAGCAAGTCACCAAGACGGAGACGCTCGCGAGAGCCTTCAACATGGTCGAAGATACACTGCACTGTGGTGCCAGAAAAGTAAGGGTCAATAACCAg                                                                                                                                                   >  2:800603/1‑277 (MQ=255)
                                                                                                                               ggAAGAACGAGGCACTTCTGGCACAAACTCGAGCGGAATATCCAGACCGTCCAGCATTTTGTCTTCCCAATCCAGGGTATGTAGGTTGAACAAGATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCACTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCa                                                                                                                                                    <  3:571943/275‑1 (MQ=255)
                                                                                                                                  agaaCGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTa                                                                                                                                            <  2:244240/280‑1 (MQ=255)
                                                                                                                                             ctacTGACAGCATCTCACGCGAAATCTCAAGAACTTCAGGCAGTTTGTCGTCCCGGTCCAGGGTATGGATGTTGAACAACATGGTACGAGGGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgctg                                                                                                                                     <  3:173307/272‑1 (MQ=255)
                                                                                                                                             cTTTTGGCAGCACGTCCCGCGGAATTTCCATGACTTCAAGCATTTTGTCGTCCCAGTCCGGGGTATGGATGTTGAACAACATGGGACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgctg                                                                                                                                     <  3:1197837/275‑1 (MQ=255)
                                                                                                                                             cTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgctg                                                                                                                                     <  1:176905/275‑1 (MQ=255)
                                                                                                                                                   ccagaaTCTCGCGCGGAATAGCCAGCATTTCCAGCATTGTCGCGTCCCAGTCCAGGGTATGGATGTTGAACGACATGGTACGAGAGGCGTTGGTGTAATCGTTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGa                                                                                                                                 <  3:729068/272‑1 (MQ=255)
                                                                                                                                                     agcaTCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGAtata                                                                                                                             <  3:353239/275‑1 (MQ=255)
                                                                                                                                                        aTCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCa                                                                                                                                                                                                                                                     >  4:34492/1‑153 (MQ=255)
                                                                                                                                                          ctcCCGTGGAATTCCCAGAACTGCCAGCGTTTTGTCTCCCCAGTCCAGGGTATGGAGGTTGAACAACAGGGTACGAGAGGGGTTGGGGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTaaa                                                                                                                   <  4:660310/280‑1 (MQ=255)
                                                                                                                                                                       tCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTACACGCTCGCGATAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCTAGAAAAGTCCGGGTCAATTAACAGACCGGTATTGTTGCGGATATAATTTTCTAAACCGTCACg                                                                                                           >  3:910531/1‑275 (MQ=255)
                                                                                                                                                                           gCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTcacca                                                                                                                                                                                                                                      <  4:231840/149‑1 (MQ=255)
                                                                                                                                                                           gCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCca                                                                                                                                                                                          <  4:712512/193‑1 (MQ=255)
                                                                                                                                                                               ttCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGATGATCCACTTCACTTTGGTGcc                                                                                                                                                                           >  2:696567/1‑204 (MQ=255)
                                                                                                                                                                                 ccAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACCTGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCGGCGGCTATAACCTTCTAAAcc                                                                                                                 >  3:1081995/1‑259 (MQ=255)
                                                                                                                                                                                 ccAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCATTCCACATGGACGCGGATCCACTTCACTTGGGTGCCCGGAAAGTACGGGCCAATCCCCAAACCGGTATTGCTGAGGATATAATCTTCTAACCGGTCACGTTTTAAATGc                                                                                                 >  1:841360/1‑275 (MQ=255)
                                                                                                                                                                                        tttGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGCTAAGCCACGTATCAACCGCACCAAACAGCAATTTACCACGCAGTGCACCCTCGCGAGAGCCTTCCACATGGTCCAGGATCCACTTCACTTTGGTGCCAGAAAAGTAACGGTAAATCACCAGACCGGTATTTGGTGGGTTAAACTTTTTTAAACCGTCACGATTTAAATtctctccgcatta                                                                                      >  4:607776/1‑270 (MQ=255)
                                                                                                                                                                                          tGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGAtt                                                                                        >  3:861625/1‑275 (MQ=255)
                                                                                                                                                                                               tcCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTaaa                                                                                                                   >  4:291979/1‑243 (MQ=255)
                                                                                                                                                                                                     tCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTac                                                                              >  3:1014471/1‑274 (MQ=255)
                                                                                                                                                                                                         gggTATGGAGGTTGAACAACATGGTACGAGAGGAGTGGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACgg                                                                         <  3:379773/275‑1 (MQ=255)
                                                                                                                                                                                                         ggaaaTGGAGGTTGAACAACATGGTACGAGAGCCGTTGGTATAATCGGTCAAATGGACACGGCCATGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACgg                                                                         <  1:218507/271‑1 (MQ=255)
                                                                                                                                                                                                          ggTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGc                                                                        >  3:1168064/1‑275 (MQ=255)
                                                                                                                                                                                                              tGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCAAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACTACGTTCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGGTTTAATCTTATAAACCGACACTTCTTAAATGCCCGCAGATTTCTGCTGTACTACGGCACTg                                                                    >  3:1270903/1‑275 (MQ=255)
                                                                                                                                                                                                               ggATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGACAACAACCAGACCGGTATTGCTGCGGATATAAACTTCTAAACCGTCACGTCATACATGCGCGCAGATTTCTACGGTAAGACGGCACTGCCAAAc                                                              >  2:311484/1‑280 (MQ=255)
                                                                                                                                                                                                                gaggTTGAACAACATGGTACGGGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGcc                                                                  <  1:348195/272‑1 (MQ=255)
                                                                                                                                                                                                                    ttGAACAACATGGTTCGAGAGGCGTTGGTGTACTCGGTCACATGTACACGGCCCTTAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTAGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAGACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATg                                                          <  2:151353/279‑1 (MQ=255)
                                                                                                                                                                                                                    ttGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTAACATGGACACGGCCCTGCGGCATTGTACAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCa                                                                 <  1:119568/272‑1 (MQ=255)
                                                                                                                                                                                                                      gAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTc                                                                                                                                                                                                            >  1:915421/1‑132 (MQ=255)
                                                                                                                                                                                                                      gAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGACCTGCCAGACaa                                                            >  1:239303/1‑275 (MQ=255)
                                                                                                                                                                                                                       aaaaacaTGGTACGGGAGGCGTTGGTGTAATCGGTCACAAGGACACGGCCCTGAGTCATTATCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACaa                                                            <  3:365319/271‑1 (MQ=255)
                                                                                                                                                                                                                         caacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATg                                                          <  1:957128/274‑1 (MQ=255)
                                                                                                                                                                                                                          aacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcaca                                                                                                                                                                                                        <  3:915421/132‑1 (MQ=255)
                                                                                                                                                                                                                          aacaTGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCAAGATAAGCCACGTATCAACCGTACCAAAGAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGt                                                                                                               <  1:684078/220‑1 (MQ=255)
                                                                                                                                                                                                                            caagggaCGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCAGAGTCATTGTCCAGATAAGCCACGTATAAACCGTACCAAACGGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGt                                                      <  1:414060/269‑1 (MQ=255)
                                                                                                                                                                                                                               ggTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGt                                                                                                                                                                                                                                                         >  1:314008/1‑78 (MQ=255)
                                                                                                                                                                                                                                   cGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCa                                                                                                                                                                                                                                                     <  3:314008/78‑1 (MQ=255)
                                                                                                                                                                                                                                      gagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACCGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCCTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGCAAATGGCGTTATAGATAgg                                            >  1:779261/1‑275 (MQ=255)
                                                                                                                                                                                                                                      gagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAgg                                            >  1:1158710/1‑275 (MQ=255)
                                                                                                                                                                                                                                      gagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGc                                       >  2:942247/1‑280 (MQ=255)
                                                                                                                                                                                                                                      gagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCCTTTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACGCCCAGACAATGGCGTTATCGATAgg                                            >  1:1123370/1‑275 (MQ=255)
                                                                                                                                                                                                                                      gagGCGTTGGTGTAATCGGTCACATGGACACGGCCCTAAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGTTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTACCGTTTTAAATGCTCGCAGATTTCTGCGGTACTACGGCAATGCAAGACAATGGCGTTATAGATAGGAtttc                                       >  2:930672/1‑278 (MQ=255)
                                                                                                                                                                                                                                         gCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCAGGGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGGGCACGCTCGCGAGAGCCTTCCAAATGACCGAGGATCCACTTCACTTCGGTGCCAGAAAAGTACGGGTCAATAACCAGCACGGTATGGCTGCGGACATAATCTTCTAAACGGGTCCGTTTTATATTCTCGCAGATTTCTGTGGTACGACGCCACCGCCAGACCATGGGGTTatagttagtgt                                          >  3:1322217/1‑271 (MQ=255)
                                                                                                                                                                                                                                          agTTGGTGTAATCGGGCACATGGACACGACCCTGAGTCAGTGTCCAGGGAAGCCACGTATCAACCGTACCAAACAGCAATTCACCTCGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGGGGATCCACTTCACTTTGATGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCa                                                                       <  3:474998/243‑1 (MQ=255)
                                                                                                                                                                                                                                           gTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTTCTGCGGATCTACTCTTCTAAACCGTCCCGTTTTAAATGCTCGCAGATTTCTTCGGTACTACGGCTCTTCCACACACTGGCGTTATCGACAGGTCTGCCGGt                                   >  4:52698/1‑279 (MQ=255)
                                                                                                                                                                                                                                           gTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAAACGTAACGTTTTAAATGCTCGCAGCTTTCTGCGGTACGACGGCACTGCCAGAACATGGGCTTTTAGATAGTCTTg                                        >  3:1334842/1‑274 (MQ=255)
                                                                                                                                                                                                                                           gTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTCCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCTGTACGACGGCACTGCCAGACAATGGCGTTATAGCTAGGCtgt                                        >  1:1182876/1‑272 (MQ=255)
                                                                                                                                                                                                                                                   aaTCGGTCACATGGACACGGTCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCcac                                                                                                                                                                                                         <  1:743770/106‑1 (MQ=255)
                                                                                                                                                                                                                                                    aTCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCACACAGCAATCCCCCCCGACGTTCACGCCCGCGAGAGCCTTCCACATCCGCGCAGATCCACCTCACTTTCGTGCCAGACAAGCACGGGCCAATCCCCCGACCGGTATTGCTGCGGACATAACATTCAACACCGTAACGTTTTGAATGCTCCCAGATTTCCACGTTATCTCGGCACTTCCAGACACCGGCCTGATAGTTGCGCTTGCCCGCGCCTTTTTcg                         >  4:763703/1‑279 (MQ=255)
                                                                                                                                                                                                                                                    aTCGGTCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCtt                              >  1:930438/1‑275 (MQ=255)
                                                                                                                                                                                                                                                      cggacacagtgaaccGGCCCGGAGTCATTGTCCACATAAACAACGTATCAACCGTACCAAACAGCAATTCACCACGACGGGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCa                       <  4:805078/266‑1 (MQ=255)
                                                                                                                                                                                                                                                         tCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCTGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTATGACGGCACTGCCAGACCATGGCGGTATAGATAGGCTTGGCGGGTTTTTTTTcc                         >  1:547876/1‑275 (MQ=255)
                                                                                                                                                                                                                                                         tCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTg                                                                                    <  2:77752/216‑1 (MQ=255)
                                                                                                                                                                                                                                                         tCACATGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCatcc                    >  2:971325/1‑277 (MQ=255)
                                                                                                                                                                                                                                                           acaTGGACACGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCa                       <  1:1334842/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cacGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGCTCGGCTGGCCGGTTTCTTTTTCCCAGCCAATAGt              >  4:248877/1‑277 (MQ=255)
                                                                                                                                                                                                                                                                   acGGCCCTGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGtt          >  2:268544/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                          tGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCAAATAGTGGTTTCACGCt   >  2:488702/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                          tGAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCCAGGATCCACTTCCCTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTTCTGCTGATATAATCTTCTAAACCTTCACGTTTTAAATCCTCGCAGATTTCTGCGGTACTACTGCACTTCCAGACCATGTCGTTATAGATAGCCTTGCCGGTTTCTTTTTCCGCGACAATTGTGGTTTCagatt   >  2:547757/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                           gAGTCATTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                                                                                                                                                                >  4:171037/1‑123 (MQ=255)
                                                                                                                                                                                                                                                                           gAGTCAGTGTCCAGATAAGCCACGTATCAACCGTAACAAACAGCAATGCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTAGAGGATCCACGTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTg  <  2:816704/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                                aTTGTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCTCGCTCGCGAGAGCCCTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTg  <  3:50282/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                
GTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTG  >  NC_000913/4116431‑4116976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: