Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,536,110 G→A G93S (GGT→AGT)  crr → glucose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,536,1100GA98.7% 855.6 / NA 234G93S (GGT→AGT) crrglucose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (114/116);  minor base C (1/2);  total (116/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

TGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCA  >  NC_000913/2535835‑2536380
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                               
     ttGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGt                                                                                                                                                                                                                                                                               <  1:216706/273‑1 (MQ=255)
      tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGt                                                                                                                                                                                                                                                                               >  1:266357/1‑272 (MQ=255)
      tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGa                                                                                                                                                                                                                                                                          >  2:45484/1‑277 (MQ=255)
      tGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATCTCCCCGCCCTCTGGCGAGACCGTCAATATCGAAGACGTGCCGGATGTCGCTTCTGCGGAAAAAATCGTTTGCGATGGTATTGCCATCAAACCAACGGGGACCAAAATCGTCGCGCTAGTATACGGGACCATTGGTCAAATATTCCAAACCAACCACGCTTTCTCTACCGAATCTGATACCGGCGTTGAACTGTTCCTCCGCTTCAGCATc                                                                                                                                                                                                                                                                            >  3:754887/1‑275 (MQ=255)
       gTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCg                                                                                                                                                                                                                                                                           >  3:313203/1‑275 (MQ=255)
       gTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGaccac                                                                                                                                                                                                                                                                     >  2:134004/1‑277 (MQ=255)
          cGATAACCTTAAATCTCTGGTTTCCGACGACAAGAAGGATAACGGAACTATTGAGATCATTTCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGa                                                                                                                                                                                                                                                                          <  3:470745/273‑1 (MQ=255)
           gATAAACTGAAATCTCTGGTTTCCGGCGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGtt                                                                                                                                                                                                                                                                  <  2:849305/279‑1 (MQ=255)
                            gttttCCGGCAAAAAAAAGAATTCCGGAAATACTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGTTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGc                                                                                                                                                                                                                                                <  4:134004/278‑1 (MQ=255)
                             gTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGa                                                                                                                                                                                                                                                    <  1:527488/275‑1 (MQ=255)
                                              ggATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTgaa                                                                                                                                                                                                                              <  2:973089/280‑1 (MQ=255)
                                                      gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCa                                                                                                                                                                                                                      >  2:1047873/1‑280 (MQ=255)
                                                      gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGATTCAAGCGTATTGCTgaagaa                                                                                                                                                                                                                           >  1:1327895/1‑275 (MQ=255)
                                                      gAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGGTGAAATGAAAGGCGAAGGCTTCAAGCGTATTGCTgaagaa                                                                                                                                                                                                                           >  1:737078/1‑275 (MQ=255)
                                                            ttGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGACAGGAGAAGGATTACAGACAATTGCTGAAGAAGTTCAg                                                                                                                                                                                                                     >  3:592548/1‑275 (MQ=255)
                                                            tagagaGCATTGCTACGCGCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGt                                                                                                                                                                                                                <  2:151390/278‑1 (MQ=255)
                                                                gaTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAATACGTGCCGGATGACGTTTTTGCGGAAAAAATCGTGGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGaaa                                                                                                                                                                                                            <  2:917480/280‑1 (MQ=255)
                                                                 aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTTTCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCCACACCGTTTAACTTACAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAg                                                                                                                                                                                                           >  2:50594/1‑280 (MQ=255)
                                                                 aTCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAg                                                                                                                                                                                                           >  4:616256/1‑280 (MQ=255)
                                                                    aTTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATc                                                                                                                                                                                                                                                                            >  1:1018081/1‑213 (MQ=255)
                                                                     ttGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTgg                                                                                                                                                                                                       <  2:773126/280‑1 (MQ=255)
                                                                      tGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGTACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTc                                                                                                                                                                                                                                             >  4:690086/1‑241 (MQ=255)
                                                                           cGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGCTGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATAGAATCTGATAGCGCCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTGGAACTTAAAGGCGAAGGCGTCAAGCGTATTGCTTAAGAAGGTACGCGCGTGACCGCTGGCGATAc                                                                                                                                                                                                 >  2:698653/1‑280 (MQ=255)
                                                                           cGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc                                                                                                                                                                                                      >  1:580844/1‑275 (MQ=255)
                                                                             ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGTTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGc                                                                                                                                                                                                      >  3:1324637/1‑273 (MQ=255)
                                                                             ctctctGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTCTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTg                                                                                                                                                                                               >  4:328486/1‑280 (MQ=255)
                                                                               ttctGGCGAAATCGTCAAGATCGAAGACGTGCCGGAAGCCGTTTTTGCGGAAAAAATCGTTGGTGAAGGTGTTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATa                                                                                                                                                                                                  <  3:614222/274‑1 (MQ=255)
                                                                                 atGGCGGGATCGTAAATTACGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAAACTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACt                                                                                                                                                                                                <  3:573620/274‑1 (MQ=255)
                                                                                  tGGCTAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTAGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCGCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCAt                                                                                                                                                                                           <  2:446067/279‑1 (MQ=255)
                                                                                  tGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAAAAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGGAACCAACCACGCATTCTCTATCGAATTTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACCACTGTTAACTGAAAGGCGAAGGATTCAAGAGTATTGCTTGAGAAGGTCAGCGCGGGAAAGTTGGCGATGCTg                                                                                                                                                                                               >  3:1146017/1‑275 (MQ=255)
                                                                                       agaTCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCAt                                                                                                                                                                                           >  1:607661/1‑274 (MQ=255)
                                                                                                    cGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTg                                                                                                                                                                              <  1:1226903/274‑1 (MQ=255)
                                                                                                     gAAGACGTGCCAGATGTCGTTTTTGCAGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGcc                                                                                                                                                                            <  1:1065229/275‑1 (MQ=255)
                                                                                                           gTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGCATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTTCTTAAGAAGGTCAGCGCTGGAAAGTCGGCGATACGGTCATTGAATTTGATCTTCCgctgct                                                                                                                                                                      >  3:1098874/1‑275 (MQ=255)
                                                                                                            tGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTa                                                                                                                                                                                                                                                                              >  1:131921/1‑171 (MQ=255)
                                                                                                             gCCGGATGTCGTGTTTGCGAAAAAAATCGTTGGTGATACGATTGCTATCAAACCAACGGGTTACAAAACTGTCGCGCCAGTAGACGGCACCAGTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAagag                                                                                                                                                               <  4:275416/280‑1 (MQ=255)
                                                                                                                   tGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                                                                                          <  2:597534/279‑1 (MQ=255)
                                                                                                                      cGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAg                                                                                                                                                           >  1:882895/1‑275 (MQ=255)
                                                                                                                        tttttGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTg                                                                                                                                                                                                        >  1:817389/1‑228 (MQ=255)
                                                                                                                        tttttGCGGAAAAAATCCTTGGTGATGTGATTGCTATCAAACCAACGGGTAACAAAATGGTCGAGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                                                                                          <  3:749809/274‑1 (MQ=255)
                                                                                                                         ttttGCGGAAAAAATCGTTGGTGGTTGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGATACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                                                                                          <  1:678316/273‑1 (MQ=255)
                                                                                                                            tGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGa                                                                                                                                                                                                    <  3:817389/228‑1 (MQ=255)
                                                                                                                                gAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTAc                                                                                                                                                 >  1:635444/1‑275 (MQ=255)
                                                                                                                                        cGTTGGTGATTGTATTGCTATCAAACCAACGGGTAACAAAAGGGTCGGGACAGTATACGGCACGATTGGTAAAATCTGTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACAACGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTc                                                                                                                                         <  3:875980/275‑1 (MQ=255)
                                                                                                                                           ggtgggtGGTATAGGTATAAAACCAAAGGGGAACAAAAGGGACGCGCCAGTAGACGGAACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTGCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt                                                                                                                                 <  4:1051209/274‑1 (MQ=255)
                                                                                                                                                aagggaTTGCTTGCAAACCAACGAGTAACAAAATGCTGGCGCCAGTAGACGGCAACATTGGTAAAAGCTTTGGAACCAACCACGCATTTTCATTCGAATCTGATAGCGGCGTTGAACGGTTCGTCCACGTCAGTAGCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCATATTGCTGAAGAAGGTCAGCGCGGGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt                                                                                                                                 <  3:1358168/270‑1 (MQ=255)
                                                                                                                                                aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                                                                                          >  4:659587/1‑250 (MQ=255)
                                                                                                                                                aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGc                                                                                                                                                          <  2:659587/250‑1 (MQ=255)
                                                                                                                                                aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAtctc                                                                                                                            <  2:190903/280‑1 (MQ=255)
                                                                                                                                                aTGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCAATTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt                                                                                                                                 <  1:946815/275‑1 (MQ=255)
                                                                                                                                                             cAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGa                                                                                                                <  2:434956/279‑1 (MQ=255)
                                                                                                                                                              aaaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGCATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTACGGTTGTTATCTCCAAAATggt                                                                                                                   >  1:809160/1‑274 (MQ=255)
                                                                                                                                                              aaaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCCACAACATTGACGaaa                                                                                                              >  4:36396/1‑280 (MQ=255)
                                                                                                                                                               aaCCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGa                                                                                                                   <  1:313203/274‑1 (MQ=255)
                                                                                                                                                                  cAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTc                                                                                                                                                    <  1:765209/238‑1 (MQ=255)
                                                                                                                                                                  cAACGGGTAACAAAATGGGCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGa                                                                                                                <  1:18093/274‑1 (MQ=255)
                                                                                                                                                                               actcgcccctccAGTCAACGGAACCATTGGTAAAATCTTGTAAACCAACCACTGATGCTCTAGCGGATCTGATCGCGGCGTTGAACTGTTCGTCCACTTCAGTCTCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                             <  4:102534/270‑1 (MQ=255)
                                                                                                                                                                                atagTCGGGCCAGTAGCAGGCAACGTTGGGAAAATCTTTGAAAACAACCACGCAGTTGCTAACGAAACTGAGAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGAGACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                             <  4:50594/276‑1 (MQ=255)
                                                                                                                                                                                acggtcccatcAGTAGACGGGACCATTGGTAAAAACTTTGGAACCCACCACGCATTCTCTATCGAATCTGCTAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACGGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                             <  4:248983/269‑1 (MQ=255)
                                                                                                                                                                                  ggTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGTGACAGCCAAATCTAACCTAGCTCCGGGTGTTATCTCAAACCTTCACGACATCAAAAAACTGAAAAAACta                                                                                          >  4:406677/1‑279 (MQ=255)
                                                                                                                                                                                   gTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTc                                                                                        >  2:716482/1‑280 (MQ=255)
                                                                                                                                                                                     cgggcCATTAGACTGCACCATTTGTAAAATCTTTGAAACCCACCACGCATTCTCTATCGCATCTGATATCGGGGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAGGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCaaa                                                                                             <  3:1220626/271‑1 (MQ=255)
                                                                                                                                                                                     cgcgCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTcc                                                                                       <  2:328486/280‑1 (MQ=255)
                                                                                                                                                                                      gcgcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCCCCAACATGGACGAAATCAAAGAACTGATCaacc                                                                                            >  3:847207/1‑272 (MQ=255)
                                                                                                                                                                                       cgcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGa                                                                                                       <  2:575849/262‑1 (MQ=255)
                                                                                                                                                                                       cgcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGa                                                                                                       >  4:575849/1‑262 (MQ=255)
                                                                                                                                                                                       cgcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACt                                                                                           >  1:342713/1‑274 (MQ=255)
                                                                                                                                                                                       cgcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCg                                                                                      >  2:330636/1‑279 (MQ=255)
                                                                                                                                                                                       cgacagaaaaCGGCACCATTGATAAAATCTTTGAAACCAACAACGCATTATCTATCGAATCTGATAGAGGCGTTGAACTGTTCGTCCACTTCAGTATCGAGACCGTTGAACGGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCgg                                                                                     <  4:443641/271‑1 (MQ=255)
                                                                                                                                                                                        gcCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTg                                                                                          <  1:988114/274‑1 (MQ=255)
                                                                                                                                                                                            gTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAGTCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCgg                                                                                     <  1:1376224/275‑1 (MQ=255)
                                                                                                                                                                                            gTAGACGGCACCATTGGTAAAATCGTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCgg                                                                                     <  1:406926/275‑1 (MQ=255)
                                                                                                                                                                                            gTAGACGGCACCATTGGTAAAATCATTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCgg                                                                                     <  1:10886/275‑1 (MQ=255)
                                                                                                                                                                                                  ggCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGACAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGAt                                                                                                 >  3:342676/1‑257 (MQ=255)
                                                                                                                                                                                                    cACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGTACGAAATCCAAGAACTGATAAAACTGTCAGGTAGCGTAACCGt                                                                         >  4:468326/1‑279 (MQ=255)
                                                                                                                                                                                                    cACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGt                                                                         >  2:120224/1‑279 (MQ=255)
                                                                                                                                                                                                         ttGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGAt                                                                                                 >  1:298589/1‑250 (MQ=255)
                                                                                                                                                                                                           ggTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGaaa                                                                 <  2:800900/280‑1 (MQ=255)
                                                                                                                                                                                                            tgaaaaGCTTTGAAACCAACCGCGCCTGCTCTATAGAATCTGGTAACGGCGTTGAAATGTTCGTCCACTGCAGTATCGACACCGTTGACCTGAAAGGCGAAGACTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGGTGGCGATACTGTCATTGACTGTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAc                                                                <  4:807460/278‑1 (MQ=255)
                                                                                                                                                                                                                  tgtttacaaccaaccCCGCTTTCTAAATCGAATGGGATAGGGGCGTTGATCTGTTCGTACACTTCAGTATCGACACCGTTTAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAAcc                                                               <  3:737078/268‑1 (MQ=255)
                                                                                                                                                                                                                     ttGAAACCAGCCACGCATTCTCGATCGAATCTGCTAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACAACGGTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCGAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCAGTAGCGTAACCGTGGGTGa                                                                   <  2:763359/268‑1 (MQ=255)
                                                                                                                                                                                                                      tGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTCCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATAAAATAACTGATCAAACTGTCCCGTATTGTAACCGTGGGTGAAACCCCGGTTact                                                      >  4:763359/1‑278 (MQ=255)
                                                                                                                                                                                                                          accaaccaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTAtc                                                                                                                              <  3:657635/204‑1 (MQ=255)
                                                                                                                                                                                                                              accaCGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCg                                                    >  1:118932/1‑274 (MQ=255)
                                                                                                                                                                                                                                  cGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACAGTTTAACTGAACGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGATTTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAATATGGACGAAATCAAAGAACTGATTAAATGGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATc                                                >  3:266823/1‑274 (MQ=255)
                                                                                                                                                                                                                                       tctcTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCCGTATCGACACCGTTGAACTGAAAGGCGAATGCTTCAAGCGTATTGCTGGAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTGGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCaagaag                                          <  1:1146017/275‑1 (MQ=255)
                                                                                                                                                                                                                                          ctATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTAGCCGCATCAAGAAGTaa                                       >  3:16954/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 aTCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCTAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTTTTGc                                >  3:239756/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 aTCTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAACGAACTGATCAAACTGTCCGGTATCGTAACCCTGGGTGAAACCCCGGTTCTCCGCACCAAGCAGTAATTCTTACCGCAg                           >  2:677886/1‑280 (MQ=255)
                                                                                                                                                                                                                                                   cTGATAGGGGCGTTTAACTGGTCGTCCACTTCAGTATCGACACCTGTGAACTGGAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGATGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCGCCAACATGGACGAAATCAAAGAACTGATCAACCTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCg                              <  3:93718/275‑1 (MQ=255)
                                                                                                                                                                                                                                                   cTGATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCg                              >  1:443390/1‑275 (MQ=255)
                                                                                                                                                                                                                                                     gATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTATCGTCACCGTGGGTGAAACCCCGGTTCACCGATCCAAGAAGTAATTTTTGcgca                             >  3:211589/1‑271 (MQ=255)
                                                                                                                                                                                                                                                     gATAGCGGCGTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCa                            <  3:436700/275‑1 (MQ=255)
                                                                                                                                                                                                                                                            gcgTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAGGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGc                                >  3:968870/1‑264 (MQ=255)
                                                                                                                                                                                                                                                              gTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGAt                                                                                                 >  3:380444/1‑197 (MQ=255)
                                                                                                                                                                                                                                                              gTTGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGaaaaa                    >  1:950646/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                tGAACTGTTCGTCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGACCTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGccc            >  2:640791/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                          gTCCACTTCAGAAACGACACCGTGGAACTGAAAGGCGAAGGGTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCa  <  4:120224/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tCCACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGaaa                                                                                                                                                                                                            <  1:431848/77‑1 (MQ=255)
                                                                                                                                                                                                                                                                            ccACTTCAGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTTATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTTCCGCCGTGAAAAATGGCGCACATCAGCGCCa  >  2:28999/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                               
TGGGTTTGTTCGATAAACTGAAATCTCTGGTTTCCGACGACAAGAAGGATACCGGAACTATTGAGATCATTGCTCCGCTCTCTGGCGAGATCGTCAATATCGAAGACGTGCCGGATGTCGTTTTTGCGGAAAAAATCGTTGGTGATGGTATTGCTATCAAACCAACGGGTAACAAAATGGTCGCGCCAGTAGACGGCACCATTGGTAAAATCTTTGAAACCAACCACGCATTCTCTATCGAATCTGATAGCGGCGTTGAACTGTTCGTCCACTTCGGTATCGACACCGTTGAACTGAAAGGCGAAGGCTTCAAGCGTATTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAAATCAAAGAACTGATCAAACTGTCCGGTAGCGTAACCGTGGGTGAAACCCCGGTTATCCGCATCAAGAAGTAATTCTTGCCGCAGTGAAAAATGGCGCCCATCGGCGCCA  >  NC_000913/2535835‑2536380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: