Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 58,228 T→G 6.9% intergenic (+49/‑246) djlA → / → yabP membrane‑anchored DnaK co‑chaperone, DNA‑binding protein/pseudogene, pentapeptide repeats‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091358,2280TG 6.9% 327.0 / 13.6 96intergenic (+49/‑246)djlA/yabPmembrane‑anchored DnaK co‑chaperone, DNA‑binding protein/pseudogene, pentapeptide repeats‑containing
Reads supporting (aligned to +/- strand):  ref base T (40/49);  new base G (2/5);  total (42/54)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GAAAGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGT  >  NC_000913/58080‑58374
                                                                                                                                                    |                                                                                                                                                  
gAAAGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCt                                                                                                                                                    >  3:388720/1‑149 (MQ=255)
gAAAGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCt                                                                                                                                                    >  1:770856/1‑149 (MQ=255)
gAAAGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAACGGTTTAAATAACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCt                                                                                                                                                    >  4:521604/1‑149 (MQ=255)
gAAAGGTTAGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCt                                                                                                                                                    >  3:698960/1‑149 (MQ=255)
 aaaGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTg                                                                                                                                                   >  4:415742/1‑149 (MQ=255)
 aaaGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTg                                                                                                                                                   >  1:670421/1‑149 (MQ=255)
   aGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGc                                                                                                                                                 >  2:242937/1‑149 (MQ=255)
    ggTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCg                                                                                                                                                <  3:352292/149‑1 (MQ=255)
    ggTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCg                                                                                                                                                <  2:4223/149‑1 (MQ=255)
       ttGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTc                                                                                                                                             <  3:23140/149‑1 (MQ=255)
       ttGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGACCGCCAGGGCggcc                                                                                                                                             <  3:85704/149‑3 (MQ=255)
         gccgccTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTCAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGACCGCCAGGGCGGCCAc                                                                                                                                           <  4:519743/149‑1 (MQ=255)
         gccgccTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGACCGCCAGGGCGGCCAc                                                                                                                                           <  3:578058/149‑1 (MQ=255)
         gccgccTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCAAATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGACCGCCAGGGCGGCCAc                                                                                                                                           <  2:687047/149‑1 (MQ=39)
          ccgccTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCAct                                                                                                                                          <  2:339698/149‑1 (MQ=255)
               tGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCttt                                                                                                                                     <  2:746253/149‑1 (MQ=255)
                 agaTGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTga                                                                                                                                   <  4:147299/149‑1 (MQ=255)
                        ggAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAgg                                                                                                                            >  4:586883/1‑149 (MQ=255)
                           gaTGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGt                                                                                                                         <  1:613621/149‑1 (MQ=255)
                           gaTGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGt                                                                                                                         <  3:290107/149‑1 (MQ=255)
                            aTGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGtt                                                                                                                        <  3:415742/149‑1 (MQ=255)
                               gCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTAc                                                                                                                     <  1:382441/149‑1 (MQ=255)
                                cGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGACCGCCAGGGCGGCCAc                                                                                                                                           >  1:273060/1‑126 (MQ=39)
                                cGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGACCGCCAGGGCGGCCAc                                                                                                                                           <  2:273060/126‑1 (MQ=39)
                                 gAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTc                                                                                                                   <  4:388720/149‑1 (MQ=255)
                                 gAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATCAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTc                                                                                                                   <  1:641671/149‑1 (MQ=255)
                                  aaGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCa                                                                                                                  <  3:443780/149‑1 (MQ=255)
                                   aGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAg                                                                                                                 >  2:285281/1‑149 (MQ=255)
                                      aGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAAt                                                                                                              <  4:568741/149‑1 (MQ=255)
                                                ggAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAgg                                                                                                    <  1:242937/149‑1 (MQ=255)
                                                ggAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCGGGCGGTCACTTCTTGGAGAAAAGGCGTTTACTCAGAATGGTGGACAgg                                                                                                    >  1:489830/1‑149 (MQ=255)
                                                    aTTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCa                                                                                                >  4:346281/1‑149 (MQ=255)
                                                      tCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAAt                                                                                              >  1:134817/1‑149 (MQ=255)
                                                      tCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAAt                                                                                              >  1:282767/1‑149 (MQ=255)
                                                      tCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAAt                                                                                              <  3:259273/149‑1 (MQ=255)
                                                       cagcagGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATg                                                                                             <  2:171067/149‑1 (MQ=255)
                                                       cagcagGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATg                                                                                             <  1:29725/149‑1 (MQ=255)
                                                        agcagGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGc                                                                                            >  2:679718/1‑149 (MQ=255)
                                                         gcagGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCa                                                                                           >  3:398910/1‑149 (MQ=255)
                                                         gcaagCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCa                                                                                           <  3:17733/145‑1 (MQ=255)
                                                          cagGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCAc                                                                                          <  4:152311/149‑1 (MQ=255)
                                                          cagGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCAc                                                                                          >  1:60180/1‑149 (MQ=255)
                                                           agGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACg                                                                                         >  4:641845/1‑149 (MQ=255)
                                                               atatGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTg                                                                                                                                                   >  4:256042/1‑87 (MQ=255)
                                                               atatGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTg                                                                                                                                                   <  3:256042/87‑1 (MQ=255)
                                                                tatGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTa                                                                                    >  4:316054/1‑149 (MQ=255)
                                                                   gAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACgg                                                                                 <  1:212267/149‑1 (MQ=255)
                                                                     gCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGa                                                                               <  4:536038/149‑1 (MQ=255)
                                                                       tGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTAc                                                                                   >  2:459983/1‑143 (MQ=255)
                                                                       tGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTAc                                                                                   <  1:459983/143‑1 (MQ=255)
                                                                        gATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAggg                                                                            <  2:739506/149‑1 (MQ=255)
                                                                           aaaGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGtt                                                                         <  4:455789/149‑1 (MQ=255)
                                                                           aaaGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGtt                                                                         <  2:651720/149‑1 (MQ=255)
                                                                             agcagcAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCt                                                                       <  3:628345/149‑1 (MQ=255)
                                                                               cagcagAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGt                                                                     >  3:91003/1‑149 (MQ=255)
                                                                                 gcagAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGc                                                                                                                           <  3:15772/93‑1 (MQ=255)
                                                                                 gcagAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGc                                                                                                                           >  4:15772/1‑93 (MQ=255)
                                                                                     aaaGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGttt                                                               >  3:703601/1‑149 (MQ=255)
                                                                                       aGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTa                                                             <  1:536361/149‑1 (MQ=255)
                                                                                          gTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAATGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCAGGGTTTACGGGAGGGGTTCTGTAGGTTTTATcg                                                          >  4:453485/1‑149 (MQ=255)
                                                                                          gTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATcg                                                          >  1:329310/1‑149 (MQ=255)
                                                                                           tttAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATcgc                                                         >  1:197348/1‑149 (MQ=255)
                                                                                           tttAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATcgc                                                         >  4:229084/1‑149 (MQ=255)
                                                                                              aaaTGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGtt                                                      <  3:316054/149‑1 (MQ=255)
                                                                                                aTGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGa                                                    <  4:91003/149‑1 (MQ=255)
                                                                                                aTGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGa                                                    <  1:616585/149‑1 (MQ=255)
                                                                                                  gACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGAcc                                                  <  3:521604/149‑1 (MQ=255)
                                                                                                  gACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGAcc                                                  >  2:635481/1‑149 (MQ=255)
                                                                                                   aCCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGAccc                                                 <  4:650500/149‑1 (MQ=255)
                                                                                                   aCCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGAccc                                                 >  1:692102/1‑149 (MQ=255)
                                                                                                    cccTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCt                                                >  2:10734/1‑149 (MQ=255)
                                                                                                     ccTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTg                                               >  3:160076/1‑149 (MQ=255)
                                                                                                      cTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGc                                              >  3:186221/1‑149 (MQ=255)
                                                                                                      cTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGc                                              <  3:358085/149‑1 (MQ=255)
                                                                                                         tAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTa                                           >  3:69762/1‑149 (MQ=255)
                                                                                                           aaTGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAg                                         <  1:285281/149‑1 (MQ=255)
                                                                                                           aaTGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAg                                         <  2:477177/149‑1 (MQ=255)
                                                                                                            atgatgCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAgg                                        <  3:229084/149‑1 (MQ=255)
                                                                                                              gatgCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGtt                                      >  1:106390/1‑149 (MQ=255)
                                                                                                              gatgCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTCAAGGtt                                      >  1:592554/1‑149 (MQ=255)
                                                                                                                tgCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTga                                    <  1:638175/149‑1 (MQ=255)
                                                                                                                tgCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTga                                    <  3:666557/149‑1 (MQ=255)
                                                                                                                  cTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAATAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGAGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTgaga                                  <  4:616403/149‑1 (MQ=255)
                                                                                                                           tGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGGCAGGCTCAATGCACGGTTTACTGGAGGGGTTCTGTAGGtt                                                                <  1:225193/110‑1 (MQ=255)
                                                                                                                           tGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGtt                                                                >  2:225193/1‑110 (MQ=255)
                                                                                                                            gCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTacgac                        <  4:398910/149‑1 (MQ=255)
                                                                                                                             cccACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTACTTAAGGTTGAGAGCTTTacgacg                       >  2:505287/1‑149 (MQ=255)
                                                                                                                              ccACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGtt                                                                                      <  4:135250/85‑1 (MQ=255)
                                                                                                                              ccACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGtt                                                                                      >  3:135250/1‑85 (MQ=255)
                                                                                                                              ccACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTAcgacga                      >  3:577886/1‑149 (MQ=255)
                                                                                                                              ccACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTAcgacga                      >  2:216999/1‑149 (MQ=255)
                                                                                                                               cACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAg                     >  1:609334/1‑149 (MQ=255)
                                                                                                                                  gATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCgg                  >  4:329593/1‑149 (MQ=255)
                                                                                                                                            ggCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATAttt        <  4:186221/149‑1 (MQ=255)
                                                                                                                                             gccgccCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTacgac                        >  1:55184/1‑132 (MQ=255)
                                                                                                                                             gccgccCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTacgac                        <  2:55184/132‑1 (MQ=255)
                                                                                                                                               cgccCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTa     <  4:529121/149‑1 (MQ=255)
                                                                                                                                               cgccCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTa     >  2:353962/1‑149 (MQ=255)
                                                                                                                                                gccCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTAc    <  2:612512/149‑1 (MQ=255)
                                                                                                                                                  ccTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGt  >  2:61150/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAAAGGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGACCCTGTAAATGATGCTGAGTAACTGCCCACGATTAAAGGTGGCCGCCCTGGCGGTCACTTCTTTGAGAAAAGGCGTTTACTCAGAATGGTGGACAGGCTCAATGCACGGTTTACGGGAGGGGTTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGT  >  NC_000913/58080‑58374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: