Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,227,051 G→C 8.2% intergenic (+83/+20) ycgJ → / ← pliG uncharacterized protein/periplasmic inhibitor of g‑type lysozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,227,0510GC 8.2% 285.2 / 14.8 85intergenic (+83/+20)ycgJ/pliGuncharacterized protein/periplasmic inhibitor of g‑type lysozyme
Reads supporting (aligned to +/- strand):  ref base G (36/42);  new base C (3/4);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

GATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAG  >  NC_000913/1226903‑1227196
                                                                                                                                                    |                                                                                                                                                 
gATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgag                                                                                                                                                   >  3:5909/1‑149 (MQ=255)
 aTGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGgaga                                                                                                                                                  <  3:333917/149‑1 (MQ=255)
    gTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTc                                                                                                                                               <  1:265654/149‑1 (MQ=255)
       aGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTcccc                                                                                                                                            <  3:110163/149‑1 (MQ=255)
          ggAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGACTCTCCCCGTc                                                                                                                                         <  2:278844/149‑1 (MQ=255)
          ggAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGACTCTCCCCGTc                                                                                                                                         <  1:35969/149‑1 (MQ=255)
           gAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGACTCTCCCCGTca                                                                                                                                        <  1:15935/149‑2 (MQ=255)
            aGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCgg                                                                                                                                       <  3:331011/149‑1 (MQ=255)
                gAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGa                                                                                                                                   <  4:9774/149‑1 (MQ=255)
                      tcaatcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                             >  3:286990/1‑149 (MQ=255)
                      tcaatcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                             <  2:357925/149‑1 (MQ=255)
                         atcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                             <  1:324783/146‑1 (MQ=255)
                         atcaaACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTa                                                                                                                             >  2:324783/1‑146 (MQ=255)
                             aaCCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGtt                                                                                                                                     >  4:497945/1‑134 (MQ=255)
                             aaCCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGtt                                                                                                                                     <  3:497945/134‑1 (MQ=255)
                             aaCCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAAtt                                                                                                                      >  4:141801/1‑149 (MQ=255)
                             aaCCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAAtt                                                                                                                      <  1:145379/149‑1 (MQ=255)
                              accaccACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAAttt                                                                                                                     <  1:653424/149‑1 (MQ=255)
                                caccacAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGa                                                                                                                   <  2:731511/149‑1 (MQ=255)
                                   cacaAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGaa                                                                                                                  >  1:129357/1‑147 (MQ=255)
                                   cacaAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGaa                                                                                                                  <  2:129357/147‑1 (MQ=255)
                                   cacaAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAata                                                                                                                >  3:486929/1‑149 (MQ=255)
                                    acaAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAatat                                                                                                               >  3:746997/1‑149 (MQ=255)
                                        aGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGAc                                                                                                           <  1:19731/149‑1 (MQ=255)
                                        aGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGAc                                                                                                           <  4:374741/149‑1 (MQ=255)
                                         gATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACg                                                                                                          >  4:180108/1‑149 (MQ=255)
                                                   atGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAttttt                                                                                                <  3:699618/149‑1 (MQ=255)
                                                   atGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAttttt                                                                                                <  3:81164/149‑1 (MQ=255)
                                                   atGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAttttt                                                                                                <  4:746997/149‑1 (MQ=255)
                                                    tgtgTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATAtttttt                                                                                               <  2:594673/149‑1 (MQ=255)
                                                          gtgagtgaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgtttt                                                                                         <  1:756719/149‑1 (MQ=255)
                                                           tgagtgaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTatat                                                                                                    >  1:87917/1‑137 (MQ=255)
                                                           tgagtgaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTatat                                                                                                    <  2:87917/137‑1 (MQ=255)
                                                            gagtgaTGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgt                                                                                       <  4:448478/149‑1 (MQ=255)
                                                                    tCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACgg                                                                               >  3:626304/1‑149 (MQ=255)
                                                                    acTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTCATCTTTTTTAGTTTTGTTTTTACgg                                                                               >  2:473411/2‑149 (MQ=255)
                                                                     cTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACggg                                                                              >  1:167295/1‑149 (MQ=255)
                                                                         tttACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGcgtc                                                                          >  4:393204/1‑149 (MQ=255)
                                                                         tttACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGcgtc                                                                          <  4:563439/149‑1 (MQ=255)
                                                                          ttACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGcgtcg                                                                         >  3:148495/1‑149 (MQ=255)
                                                                              aTTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTc                                                                     >  1:486769/1‑149 (MQ=255)
                                                                               ttATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCg                                                                    >  2:393141/1‑149 (MQ=255)
                                                                                tATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTGATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCgg                                                                   <  4:203165/149‑1 (MQ=255)
                                                                                  tGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgtt                                                                                      >  3:73497/1‑128 (MQ=255)
                                                                                  tGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAgttttgtt                                                                                      <  4:73497/128‑1 (MQ=255)
                                                                                     tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTa                                                                                  <  3:394894/129‑1 (MQ=255)
                                                                                     tCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTa                                                                                  >  4:394894/1‑129 (MQ=255)
                                                                                      cTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGc                                                             >  1:267628/1‑149 (MQ=255)
                                                                                        aTTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCaa                                                           <  3:357656/149‑1 (MQ=255)
                                                                                          tCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAaca                                                         >  4:318366/1‑149 (MQ=255)
                                                                                             tCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCgg                                                                   >  2:509742/1‑136 (MQ=255)
                                                                                             tCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCgg                                                                   <  1:509742/136‑1 (MQ=255)
                                                                                             tCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTc                                                      >  1:360505/1‑149 (MQ=255)
                                                                                             tCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTc                                                      >  3:504203/1‑149 (MQ=255)
                                                                                                ttAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCa                                                   >  2:453258/1‑149 (MQ=255)
                                                                                                ttAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCa                                                   >  1:233083/1‑149 (MQ=255)
                                                                                                  aaCCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGc                                                 <  1:205528/149‑1 (MQ=255)
                                                                                                            gCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGc                                       <  3:393204/149‑1 (MQ=255)
                                                                                                             cGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGcc                                      >  1:103109/1‑149 (MQ=255)
                                                                                                             cGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGcc                                      >  1:22026/1‑149 (MQ=255)
                                                                                                              gACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                     <  4:179674/149‑1 (MQ=255)
                                                                                                              gACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                     >  2:644158/1‑149 (MQ=255)
                                                                                                               aCAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                    >  4:660044/1‑149 (MQ=255)
                                                                                                                cAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGa                                   >  3:168183/1‑149 (MQ=255)
                                                                                                                 agagagGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGaa                                  <  2:267628/149‑1 (MQ=255)
                                                                                                                     aggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAg                              <  4:445808/149‑1 (MQ=255)
                                                                                                                      ggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAgg                             <  4:97359/149‑1 (MQ=255)
                                                                                                                      ggagTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAgg                             <  4:51679/149‑1 (MQ=255)
                                                                                                                        agTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGga                           <  1:256578/149‑1 (MQ=255)
                                                                                                                        agTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGga                           <  2:360505/149‑1 (MQ=255)
                                                                                                                          tGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagt                         <  1:388081/149‑1 (MQ=255)
                                                                                                                               cATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTa                    <  1:773021/149‑1 (MQ=255)
                                                                                                                                aTTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCaa                                                           >  1:346931/1‑109 (MQ=255)
                                                                                                                                aTTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCaa                                                           <  2:346931/109‑1 (MQ=255)
                                                                                                                                 ttAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACCTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAtt                  >  4:117537/1‑149 (MQ=255)
                                                                                                                                 ttAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAtt                  >  4:702520/1‑149 (MQ=255)
                                                                                                                                 ttAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAtt                  <  3:318366/149‑1 (MQ=255)
                                                                                                                                       tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc              <  3:524333/147‑1 (MQ=255)
                                                                                                                                       tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc              >  4:524333/1‑147 (MQ=255)
                                                                                                                                         tATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGa          <  4:197550/149‑1 (MQ=255)
                                                                                                                                         tATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGa          <  4:5909/149‑1 (MQ=255)
                                                                                                                                          ccgACGGGGACTCTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGa          <  4:693214/146‑1 (MQ=255)
                                                                                                                                          ccgACGGGGACTCTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGa          >  3:693214/3‑148 (MQ=255)
                                                                                                                                          ccgACGGGGACTCTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGAc         >  4:746556/3‑149 (MQ=255)
                                                                                                                                           cgACGGGGACTCTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACt        >  2:645138/2‑149 (MQ=255)
                                                                                                                                                 ggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAg  >  1:364537/1‑149 (MQ=255)
                                                                                                                                                 ggAGAGTCCCCGTCGGTTGACATTTAATTAATTTGAATGTCGACGTTATATTTTTTAGTTTTGTTTATACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAg  >  3:635916/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GATGGTAAGCGGAGTGGAAAAATCAATCAAACCACCACAAAGATGTTATTTATGTGTCGTGAGTGATGTCTTGTTTACATTATGTTCTATTCGTCATTAACCTCTTGAGCGACAGAGAGGAGTGGGGCATTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAG  >  NC_000913/1226903‑1227196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: