Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,449,050 T→A 11.6% intergenic (+32/+26) feaB → / ← tynA phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,0500TA11.6% 232.7 / 18.5 77intergenic (+32/+26)feaB/tynAphenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base T (39/29);  new base A (5/4);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

TTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAT  >  NC_000913/1448902‑1449194
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ttAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCt                                                                                                                                                  >  2:105017/1‑149 (MQ=255)
  aaTTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTgg                                                                                                                                                >  2:704827/1‑149 (MQ=255)
      gACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGcc                                                                                                                                            <  2:32357/149‑1 (MQ=255)
      gACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGcc                                                                                                                                            <  2:724362/149‑1 (MQ=255)
         gCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGtt                                                                                                                                         <  3:619791/149‑1 (MQ=255)
          cTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGttt                                                                                                                                        <  4:140148/149‑1 (MQ=255)
           tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGtttt                                                                                                                                       <  4:720547/149‑1 (MQ=255)
           tAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGtttt                                                                                                                                       <  1:508424/149‑1 (MQ=255)
               ttACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAACACGGCACCAGGTGCCGtttttt                                                                                                                                     <  2:529277/147‑1 (MQ=255)
               ttACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTg                                                                                                                                   >  3:380322/1‑149 (MQ=255)
               ttACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGtttttt                                                                                                                                     >  1:529277/1‑147 (MQ=255)
                  ccGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCt                                                                                                                                <  4:415399/149‑1 (MQ=255)
                  ccGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCt                                                                                                                                <  4:659161/149‑1 (MQ=255)
                         gtggGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGtt                                                                                                                                         >  3:244914/1‑133 (MQ=255)
                         gtggGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGtt                                                                                                                                         <  4:244914/133‑1 (MQ=255)
                         gtggGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACaa                                                                                                                         >  4:431122/1‑149 (MQ=255)
                          tggGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGtttt                                                                                                                                       >  3:41010/1‑134 (MQ=255)
                          tggGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCAGGTGCCGtttt                                                                                                                                       <  4:41010/134‑1 (MQ=255)
                             gATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCAc                                                                                                                     <  1:704827/149‑1 (MQ=255)
                                 aaGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAt                                                                                                                 >  1:10737/1‑149 (MQ=255)
                                  aGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtc                                                                                                                <  1:489255/149‑1 (MQ=255)
                                      gTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtctttc                                                                                                            <  2:550163/149‑1 (MQ=255)
                                       tCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTAtctttct                                                                                                           >  2:626424/1‑149 (MQ=255)
                                         aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTttcttc                                                                                                         >  4:370446/1‑149 (MQ=255)
                                         aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTttcttc                                                                                                         >  4:206737/1‑149 (MQ=255)
                                           gAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAg                                                                                                       <  3:673581/149‑1 (MQ=255)
                                               gggCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGcc                                                                                                   >  3:103761/1‑149 (MQ=255)
                                                ggCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGccc                                                                                                  <  4:611726/149‑1 (MQ=255)
                                                 gCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGcccc                                                                                                 >  2:1395/1‑149 (MQ=255)
                                                   cGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTa                                                                                               >  3:543799/1‑149 (MQ=255)
                                                     tGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGc                                                                                             <  3:513523/149‑1 (MQ=255)
                                                          ttGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCGCTATCGTTgg                                                                                        <  2:185805/149‑1 (MQ=255)
                                                               cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGttt                                                                                   >  4:525155/1‑149 (MQ=255)
                                                                     tggctggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTGTTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCaa                                                                             >  4:44444/1‑149 (MQ=255)
                                                                         tggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCt                                                                                                                                                  >  4:115412/1‑76 (MQ=255)
                                                                         tggACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCt                                                                                                                                                  <  3:115412/76‑1 (MQ=255)
                                                                                ttGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCAt                                                                  <  3:206737/149‑1 (MQ=255)
                                                                                    tgtgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGtt                                                              >  1:181924/1‑149 (MQ=255)
                                                                                     gtgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGttt                                                             <  2:294286/149‑1 (MQ=255)
                                                                                      tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGttt                                                                                                                                        <  2:392469/73‑1 (MQ=255)
                                                                                      tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGttt                                                                                                                                        >  1:392469/1‑73 (MQ=255)
                                                                                      tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATgg                                                                <  3:154381/145‑1 (MQ=255)
                                                                                      tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATgg                                                                >  4:154381/1‑145 (MQ=255)
                                                                                      tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTc                                                            >  2:692728/1‑149 (MQ=255)
                                                                                       gAAACTAAGTCGGTGTGTGTACGGTATTAATCTGATTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTca                                                           <  1:139227/149‑1 (MQ=255)
                                                                                         aaCTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaagaa                                                                         >  1:334234/1‑133 (MQ=255)
                                                                                         aaCTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaagaa                                                                         <  2:334234/133‑1 (MQ=255)
                                                                                          aCTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagca                                                        >  3:175895/1‑149 (MQ=255)
                                                                                               gTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTa                                                   >  1:614796/1‑149 (MQ=255)
                                                                                               gTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTa                                                   <  2:402065/149‑1 (MQ=255)
                                                                                                   gtgtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTa                                               >  4:8964/1‑149 (MQ=255)
                                                                                                   gtgtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTa                                               >  1:414737/1‑149 (MQ=255)
                                                                                                   gtgtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTa                                               <  1:739925/149‑1 (MQ=255)
                                                                                                     gtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTAcc                                             >  1:335805/1‑149 (MQ=255)
                                                                                                      tgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCGAAGAAGTTCCATGGTTTCAGCAGAGTATGTAccc                                            <  1:105017/149‑1 (MQ=255)
                                                                                                        tgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCAt                                          >  1:774131/1‑149 (MQ=255)
                                                                                                             ggTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcggt                                     >  3:2990/1‑149 (MQ=255)
                                                                                                             ggTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcggt                                     >  4:630684/1‑149 (MQ=255)
                                                                                                                aTTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcggtcgg                                  <  4:2990/149‑1 (MQ=255)
                                                                                                                aTTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcggtcgg                                  >  1:272264/1‑149 (MQ=255)
                                                                                                                    aTCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATa                              >  1:27962/1‑149 (MQ=255)
                                                                                                                     tCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATaa                             >  1:449960/1‑149 (MQ=255)
                                                                                                                     tCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATaa                             >  4:222744/1‑149 (MQ=255)
                                                                                                                          ttCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGc                        >  3:169851/1‑149 (MQ=255)
                                                                                                                              cTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCa                                                                   <  2:673723/102‑1 (MQ=255)
                                                                                                                              cTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCa                                                                   >  1:673723/1‑102 (MQ=255)
                                                                                                                               tCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCGGAGTATGTACCCATTCGGTCGGCATAATCGGCCActc                   >  1:228774/1‑149 (MQ=255)
                                                                                                                               tCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCActc                   >  2:519410/1‑149 (MQ=255)
                                                                                                                                 ttAAGTAAACAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCActctc                 >  4:653913/2‑148 (MQ=255)
                                                                                                                                  tAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTc                <  2:100070/149‑1 (MQ=255)
                                                                                                                                      aaaaaaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgcg            >  1:459397/2‑149 (MQ=255)
                                                                                                                                       aaaaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgcgc           >  1:282498/1‑149 (MQ=255)
                                                                                                                                         aaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCgg         <  2:282498/149‑1 (MQ=255)
                                                                                                                                         aaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCgg         >  2:138942/1‑149 (MQ=255)
                                                                                                                                         aaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCgg         >  3:381497/1‑149 (MQ=255)
                                                                                                                                         aaaaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCgg         <  1:1395/149‑1 (MQ=255)
                                                                                                                                         aaaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCgg         >  2:493603/1‑149 (MQ=255)
                                                                                                                                         aaaaCGGCACCAGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCgg         >  2:276281/1‑149 (MQ=255)
                                                                                                                                           aaCGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGc       >  1:177164/1‑149 (MQ=255)
                                                                                                                                                cACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAt  <  3:71674/149‑1 (MQ=255)
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TTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCGCGGGCCACAT  >  NC_000913/1448902‑1449194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑