Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,456,819 T→A 8.1% I130I (ATT→ATA)  paaE → ring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,456,8190TA 8.1% 251.6 / 11.0 74I130I (ATT→ATA) paaEring 1,2‑phenylacetyl‑CoA epoxidase, NAD(P)H oxidoreductase component
Reads supporting (aligned to +/- strand):  ref base T (36/32);  new base A (4/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTC  >  NC_000913/1456680‑1456967
                                                                                                                                           |                                                                                                                                                    
cccGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGccacc                                                                                                                                             >  2:124623/1‑149 (MQ=255)
    cgAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACtt                                                                                                                                         <  1:640376/149‑1 (MQ=255)
     gAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACttt                                                                                                                                        >  4:118853/1‑149 (MQ=255)
         acaTCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATc                                                                                                                                                   >  2:558999/1‑134 (MQ=255)
         acaTCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATc                                                                                                                                                   <  1:558999/134‑1 (MQ=255)
         acaTCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACaa                                                                                                                                    >  4:124867/1‑149 (MQ=255)
           aTCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAAc                                                                                                                                  >  3:495117/1‑149 (MQ=255)
               gCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAg                                                                                                                              >  3:520392/1‑149 (MQ=255)
                ccAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGc                                                                                                                             <  1:589742/149‑1 (MQ=255)
                 cAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGcc                                                                                                                            >  3:703094/1‑149 (MQ=255)
                   gggTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTg                                                                                                                          <  3:578950/149‑1 (MQ=255)
                      tATGACGGTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGAAGGCCGAACGCCAGGGGCGCTAGCTGGCAATTGCAGCAGAATCAGGTATTACGGCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGaaa                                                                                                                       <  2:613636/149‑1 (MQ=255)
                      tATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCTGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGaaa                                                                                                                       <  2:670625/149‑1 (MQ=255)
                          aCGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCa                                                                                                                   <  4:174915/149‑1 (MQ=255)
                            gCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGt                                                                                                                 <  3:37730/149‑1 (MQ=255)
                             cTGGAGGTCATGGTGCCGCAGGGGCATTTCCGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGtt                                                                                                                <  4:118388/149‑1 (MQ=255)
                               ggAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCa                                                                                                              <  2:275392/149‑1 (MQ=255)
                                   gTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCAGAAAGTCAGTTCACCCt                                                                                                          >  1:42927/1‑149 (MQ=255)
                                    tCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTg                                                                                                         >  1:399577/1‑149 (MQ=255)
                                      aTGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCGATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTTAAAGTCAGTTCACCCTGAt                                                                                                       <  3:597936/149‑1 (MQ=255)
                                      aTGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGAt                                                                                                       <  4:236558/149‑1 (MQ=255)
                                      aTGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGAt                                                                                                       >  1:767002/1‑149 (MQ=255)
                                       tGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATc                                                                                                      <  4:481417/149‑1 (MQ=255)
                                        ggTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCt                                                                                                     >  1:512749/1‑149 (MQ=255)
                                         gTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTa                                                                                                    >  3:436924/1‑149 (MQ=255)
                                              gCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTa                                                                                               >  2:133941/1‑149 (MQ=255)
                                                aGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAAc                                                                                             >  2:251775/1‑149 (MQ=255)
                                                aGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAAc                                                                                             <  2:123559/149‑1 (MQ=255)
                                                   ggCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGt                                                                                          >  4:606560/1‑149 (MQ=255)
                                                    gCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTa                                                                                         >  2:420380/1‑149 (MQ=255)
                                                     cATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTAc                                                                                        <  2:265381/149‑1 (MQ=255)
                                                           ggCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTAccagcca                                                                                  <  4:703094/149‑1 (MQ=255)
                                                             cTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCaga                                                                                <  1:494011/149‑1 (MQ=255)
                                                              tATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCagag                                                                               >  2:513937/1‑149 (MQ=255)
                                                                tCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCa                                                                             >  1:323842/1‑149 (MQ=255)
                                                                   gCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCatga                                                                          >  4:327769/1‑149 (MQ=255)
                                                                            cGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGcc                                                                 <  1:124623/149‑1 (MQ=255)
                                                                             gAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGcga                                                                <  4:520392/149‑3 (MQ=255)
                                                                              aaCGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAg                                                               <  4:482952/149‑1 (MQ=255)
                                                                               aCGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAgg                                                              <  3:606560/149‑1 (MQ=255)
                                                                                     ggggCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTgg                                                        <  2:767002/149‑1 (MQ=255)
                                                                                      gggCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGc                                                       >  4:146618/1‑149 (MQ=255)
                                                                                            tATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCt                                                 <  2:457238/149‑1 (MQ=255)
                                                                                                tGGCAATTGCAGCAGGATCAGGTATTACGCCAATGGTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGaaa                                             <  4:495117/149‑1 (MQ=255)
                                                                                                tGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGaaa                                             >  4:371738/1‑149 (MQ=255)
                                                                                                 ggCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTc                                                                    >  2:557291/1‑125 (MQ=255)
                                                                                                 ggCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTc                                                                    <  1:557291/125‑1 (MQ=255)
                                                                                                 ggCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAg                                            >  4:644563/1‑149 (MQ=255)
                                                                                                  gCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGa                                           >  3:107014/1‑149 (MQ=255)
                                                                                                    aaTTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACa                                         >  1:390269/1‑149 (MQ=255)
                                                                                                          agcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATc                                   >  4:397168/1‑149 (MQ=255)
                                                                                                          agcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATc                                   >  3:648587/1‑149 (MQ=255)
                                                                                                           gcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGa                                                                                                        >  3:545689/1‑79 (MQ=255)
                                                                                                           gcagGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGa                                                                                                        <  4:545689/79‑1 (MQ=255)
                                                                                                               gATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAg                              >  3:736779/1‑149 (MQ=255)
                                                                                                                      tATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTa                        >  2:249909/1‑148 (MQ=255)
                                                                                                                      tATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTa                        <  1:249909/148‑1 (MQ=255)
                                                                                                                        ttACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAg                     <  1:586421/149‑1 (MQ=255)
                                                                                                                          aCGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAgtt                   <  3:146618/149‑1 (MQ=255)
                                                                                                                           cGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAgttg                  >  4:205176/1‑149 (MQ=255)
                                                                                                                           cGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAgttg                  >  4:234428/1‑149 (MQ=255)
                                                                                                                            gCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCat                                                                            >  2:650654/1‑90 (MQ=255)
                                                                                                                            gCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCat                                                                            <  1:650654/90‑1 (MQ=255)
                                                                                                                            gCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAgttgt                 >  1:650233/1‑149 (MQ=255)
                                                                                                                             ccAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAgttgtt                <  2:135177/149‑1 (MQ=255)
                                                                                                                                gtggtGGCGATAATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTtgtg             >  3:596701/5‑149 (MQ=255)
                                                                                                                                 tggtGGCGATAATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGc            >  1:592630/4‑149 (MQ=255)
                                                                                                                                   gtGGCGATAATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCt                                 >  2:666592/2‑126 (MQ=255)
                                                                                                                                   gtGGCGATAATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCt                                 <  1:666592/125‑1 (MQ=255)
                                                                                                                                    tGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCAtt         >  1:741962/1‑149 (MQ=255)
                                                                                                                                    tGGCGATAATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTgg                                                        <  1:749330/102‑1 (MQ=255)
                                                                                                                                    tGGCGATAATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTgg                                                        >  2:749330/1‑102 (MQ=255)
                                                                                                                                      gCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCAtttt       >  4:304899/1‑149 (MQ=255)
                                                                                                                                         aTTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTtcag    <  3:254299/149‑1 (MQ=255)
                                                                                                                                           tATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGTGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTtcagtc  <  2:390269/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
CCCGCGAACACATCCGCCAGGGTATGACGCTGGAGGTCATGGTGCCGCAGGGGCATTTCGGCTATCAGCCGCAGGCCGAACGCCAGGGGCGCTATCTGGCAATTGCAGCAGGATCAGGTATTACGCCAATGCTGGCGATTATCGCCACCACTTTACAAACCGAGCCTGAAAGTCAGTTCACCCTGATCTACGGTAACCGTACCAGCCAGAGCATGATGTTTCGCCAGGCACTGGCAGACCTGAAAGACAAATATCCTCAGCGTTTACAGTTGTTGTGCATTTTCAGTC  >  NC_000913/1456680‑1456967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑