Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,928,802 G→T 5.4% T13K (ACG→AAG)  ptrB ← protease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,928,8020GT 5.4% 328.3 / 7.4 92T13K (ACG→AAG) ptrBprotease II
Reads supporting (aligned to +/- strand):  ref base G (44/43);  new base T (2/3);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

GATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGAT  >  NC_000913/1928657‑1928948
                                                                                                                                                 |                                                                                                                                                  
gATGCGATCCTGCAGGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCa                                                                                                                                                 >  3:58046/1‑149 (MQ=255)
  tGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATg                                                                                                                                               >  2:45420/1‑149 (MQ=255)
    cGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGc                                                                                                                                             <  4:697075/149‑1 (MQ=255)
     gATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCg                                                                                                                                            >  2:691706/1‑149 (MQ=255)
      aTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGt                                                                                                                                           >  3:634077/1‑149 (MQ=255)
         cTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTggg                                                                                                                                        <  4:58046/149‑1 (MQ=255)
          tGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATg                                                                                                                                               >  2:581376/1‑141 (MQ=255)
          tGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATg                                                                                                                                               <  1:581376/141‑1 (MQ=255)
                gCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcg                                                                                                                                 >  4:723687/1‑149 (MQ=255)
                 cTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgg                                                                                                                                <  2:416365/149‑1 (MQ=255)
                           ggCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTg                                                                                                                      <  3:264303/149‑1 (MQ=255)
                            gCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                                                                                                     >  4:306150/1‑149 (MQ=255)
                             ccATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGt                                                                                                                    <  3:723687/149‑1 (MQ=255)
                              cATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                                   <  4:554245/149‑1 (MQ=255)
                               aTCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg                                                                                                                  >  1:656075/1‑149 (MQ=255)
                               aTCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg                                                                                                                  >  1:551543/1‑149 (MQ=255)
                                tCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGc                                                                                                                 >  2:158056/1‑149 (MQ=255)
                                 cACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                                                                                                                <  2:640093/149‑1 (MQ=255)
                                  aCCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCAt                                                                                                               <  1:173590/149‑1 (MQ=255)
                                     cGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGtt                                                                                                            <  4:634077/149‑1 (MQ=255)
                                        tGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTAtt                                                                                                         <  1:691706/149‑1 (MQ=255)
                                            cGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGCGGGGACTGCGGGCGGCTTTTGGTAGCATGTTATTGttc                                                                                                     >  3:527394/1‑149 (MQ=255)
                                             gTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttct                                                                                                    <  4:13107/149‑1 (MQ=255)
                                              tAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctt                                                                                                   >  1:445443/1‑149 (MQ=255)
                                              tAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctt                                                                                                   >  4:452323/1‑149 (MQ=255)
                                              tAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctt                                                                                                   >  4:690942/1‑149 (MQ=255)
                                                   aTTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTgg                                                                                              <  3:598237/149‑1 (MQ=255)
                                                    ttttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGt                                                                                             <  1:45420/149‑1 (MQ=255)
                                                    ttttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGCCTATTGGTAGCATGTTATTGTTCTTTCTGGt                                                                                             <  4:464741/149‑1 (MQ=255)
                                                     tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGtt                                                                                            <  3:709976/149‑1 (MQ=255)
                                                     tttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGtt                                                                                            >  3:166482/1‑149 (MQ=255)
                                                      ttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTg                                                                                           <  1:157704/149‑1 (MQ=255)
                                                       tCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGa                                                                                          <  2:567255/149‑1 (MQ=255)
                                                        cTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaa                                                                                         >  4:362483/1‑149 (MQ=255)
                                                         ttgttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaaa                                                                                        <  2:324723/149‑1 (MQ=255)
                                                         ttgttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaaa                                                                                        >  3:2060/1‑149 (MQ=255)
                                                             tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATc                                                                                    <  3:225128/149‑1 (MQ=255)
                                                                aGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTta                                                                                 <  4:70479/149‑1 (MQ=255)
                                                                 ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTtat                                                                                >  3:274167/1‑149 (MQ=255)
                                                                       ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGt                                                                          <  3:547007/149‑1 (MQ=255)
                                                                       ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGt                                                                          <  1:128785/149‑1 (MQ=255)
                                                                        cAGGCCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGGGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                         >  2:672840/1‑149 (MQ=255)
                                                                        cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATaa                                                                              <  1:727024/144‑1 (MQ=255)
                                                                        cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATaa                                                                              >  2:727024/1‑144 (MQ=255)
                                                                        cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                         >  2:531442/1‑149 (MQ=255)
                                                                        cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                         >  4:226806/1‑149 (MQ=255)
                                                                        cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                         <  4:443878/149‑1 (MQ=255)
                                                                         aGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaaa                                                                                        <  1:672976/133‑1 (MQ=255)
                                                                         aGGACTACTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaaa                                                                                        >  2:672976/1‑133 (MQ=255)
                                                                          ggACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGc                                                                       >  3:73649/1‑149 (MQ=255)
                                                                              ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   <  1:753273/149‑1 (MQ=255)
                                                                              ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  3:223908/1‑149 (MQ=255)
                                                                              ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  2:659606/1‑149 (MQ=255)
                                                                              ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  2:310363/1‑149 (MQ=255)
                                                                              ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  3:747625/1‑149 (MQ=255)
                                                                              ttCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTGGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaaa                                                                   >  2:725372/1‑149 (MQ=255)
                                                                               tCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                                  <  1:57290/149‑1 (MQ=255)
                                                                                 tGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTc                                                                >  3:204668/1‑149 (MQ=255)
                                                                                  ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                                   >  2:376694/1‑97 (MQ=255)
                                                                                  ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                                   <  1:376694/97‑1 (MQ=255)
                                                                                  ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                                  >  4:238355/1‑146 (MQ=255)
                                                                                  ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                                  <  3:238355/146‑1 (MQ=255)
                                                                                   gCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCAc                                                              <  3:399110/149‑1 (MQ=255)
                                                                                   gCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCAc                                                              <  1:659606/149‑1 (MQ=255)
                                                                                     tGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttc                                                                                                 >  3:452692/1‑112 (MQ=255)
                                                                                     tGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctttc                                                                                                 <  4:452692/112‑1 (MQ=255)
                                                                                        gaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCgggg                                                         >  3:679258/1‑149 (MQ=255)
                                                                                               tATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAg                                                  <  4:274167/149‑1 (MQ=255)
                                                                                                       cGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAg                                          <  3:690942/149‑1 (MQ=255)
                                                                                                       cGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAg                                          >  2:558364/1‑149 (MQ=255)
                                                                                                         cAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAggg                                        <  4:223908/149‑1 (MQ=255)
                                                                                                         cAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAggg                                        <  3:537954/149‑1 (MQ=255)
                                                                                                           gCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGa                                      <  3:596160/149‑1 (MQ=255)
                                                                                                               gtagtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCGCCGGGGATGCAAGCGAAACAGGGGAGTCa                                  >  2:376160/1‑149 (MQ=255)
                                                                                                                  gtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTg                               >  2:276731/1‑149 (MQ=255)
                                                                                                                    aaTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCt                             <  1:725372/149‑1 (MQ=255)
                                                                                                                     aTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGa                                      <  4:726669/139‑1 (MQ=255)
                                                                                                                     aTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGa                                      >  3:726669/1‑139 (MQ=255)
                                                                                                                     aTTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCtt                            <  3:2049/149‑1 (MQ=255)
                                                                                                                        ctCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAga                         >  3:30439/2‑149 (MQ=255)
                                                                                                                            aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAgatgat                     >  2:743301/1‑149 (MQ=255)
                                                                                                                              gcgcGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGAtgatga                   <  3:452323/149‑1 (MQ=255)
                                                                                                                                gcgTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACa                 >  1:759790/1‑149 (MQ=255)
                                                                                                                                gcgTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACa                 >  1:491857/1‑149 (MQ=255)
                                                                                                                                     tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATc                                                                                    >  2:393134/1‑77 (MQ=255)
                                                                                                                                     tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATc                                                                                    <  1:393134/77‑1 (MQ=255)
                                                                                                                                       gCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAggg                                        <  2:601537/119‑1 (MQ=255)
                                                                                                                                       gCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAggg                                        >  1:601537/1‑119 (MQ=255)
                                                                                                                                          aTGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGgcg       >  2:123085/1‑149 (MQ=255)
                                                                                                                                           tGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGgcgc      <  1:32095/149‑1 (MQ=255)
                                                                                                                                            gAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGcgcg     >  1:770326/1‑149 (MQ=255)
                                                                                                                                               gCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGat  <  3:226806/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCGGAT  >  NC_000913/1928657‑1928948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: