Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,858 G→C 5.7% intergenic (‑100/+15) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,8580GC 5.7% 291.9 / 5.4 83intergenic (‑100/+15)yecJ/azuCDUF2766 family protein/acid‑inducible small membrane‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (39/39);  new base C (2/3);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTT  >  NC_000913/1987712‑1988001
                                                                                                                                                  |                                                                                                                                                
aCCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGa                                                                                                                                                >  1:392933/1‑149 (MQ=255)
   acaTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCa                                                                                                                                             >  4:550760/1‑149 (MQ=255)
     aTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAgg                                                                                                                                           >  1:486748/1‑149 (MQ=255)
      tCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑t                                                                                                                                         >  1:183981/1‑149 (MQ=255)
      tCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑t                                                                                                                                         >  1:739533/1‑149 (MQ=255)
       cAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑tt                                                                                                                                        <  2:186529/149‑1 (MQ=255)
        aGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑ttt                                                                                                                                       <  2:660255/149‑1 (MQ=255)
         gAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGG‑tttt                                                                                                                                      <  2:208815/149‑1 (MQ=255)
         gAAACCAATTCCTGATCGGCACTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGG‑tttt                                                                                                                                      <  3:514496/149‑1 (MQ=255)
          aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑ttttt                                                                                                                                     <  3:182035/149‑1 (MQ=255)
           aaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑tttttt                                                                                                                                    <  3:550760/149‑1 (MQ=255)
              cAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACAC‑AAAAAACCTGCTCCGGCAGG‑tttttttgtg                                                                                                                                <  4:569039/149‑5 (MQ=255)
                  tCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCg                                                                                                                             <  3:11338/149‑1 (MQ=255)
                     tGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGaa                                                                                                                          <  2:726629/149‑1 (MQ=255)
                     tGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGaa                                                                                                                          >  1:503648/1‑149 (MQ=255)
                          ggCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACatat                                                                                                                     >  4:565579/1‑149 (MQ=255)
                             aTTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGc                                                                                                                  >  2:760489/1‑149 (MQ=255)
                             aTTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGc                                                                                                                  >  1:720618/1‑149 (MQ=255)
                             aTTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGc                                                                                                                  <  2:392933/149‑1 (MQ=255)
                                  gCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGc                                                                                                             >  4:398175/1‑149 (MQ=255)
                                          gACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTc                                                                                                     <  3:363834/149‑1 (MQ=255)
                                          gACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTACTGGACCATATTGACTAGCGGTACGTc                                                                                                     >  1:297104/1‑149 (MQ=255)
                                              tCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTg                                                                                                 <  2:765037/149‑1 (MQ=255)
                                              tCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTg                                                                                                 >  1:251690/1‑149 (MQ=255)
                                                gTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGaa                                                                                               <  1:15217/149‑1 (MQ=255)
                                                gTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGaa                                                                                               <  2:183981/149‑1 (MQ=255)
                                                 tAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAAc                                                                                              <  3:135641/149‑1 (MQ=255)
                                                       ccTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTg                                                                                        <  2:251690/149‑1 (MQ=255)
                                                        cTGATAGCATTGTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGc                                                                                       >  1:574562/1‑149 (MQ=255)
                                                          gATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCaa                                                                                     >  1:439772/1‑149 (MQ=255)
                                                           aTAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCATGGCGGTACGTCTTTGAACGTCTTGCAAt                                                                                    >  4:194204/1‑149 (MQ=255)
                                                            tAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa                                                                                   >  3:753582/1‑149 (MQ=255)
                                                            tAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATa                                                                                   <  1:760489/149‑1 (MQ=255)
                                                             aGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAg                                                                                  <  3:389072/149‑1 (MQ=255)
                                                             aGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAg                                                                                  >  3:391127/1‑149 (MQ=255)
                                                               cATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑tttttt                                                                                                                                    <  2:604998/97‑1 (MQ=255)
                                                               cATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑tttttt                                                                                                                                    >  1:604998/1‑97 (MQ=255)
                                                               cATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGtt                                                                                >  4:369927/1‑149 (MQ=255)
                                                                 ttttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                              <  2:490207/149‑1 (MQ=255)
                                                                 ttttGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                              <  4:575615/149‑1 (MQ=255)
                                                                     gAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGTAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGaa                                                                          <  2:237225/149‑1 (MQ=255)
                                                                     gAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGaa                                                                          <  2:73201/149‑1 (MQ=255)
                                                                     gAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGaa                                                                          <  2:546718/149‑1 (MQ=255)
                                                                         gCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATa                                                                      >  4:742604/1‑149 (MQ=255)
                                                                            gTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACtt                                                                   >  3:704441/1‑149 (MQ=255)
                                                                                   tagCGACTTCAAATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGa                                                            >  3:443057/1‑149 (MQ=255)
                                                                                     gCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAAc                                                                                                                         >  4:590825/1‑86 (MQ=255)
                                                                                     gCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAAc                                                                                                                         <  3:590825/86‑1 (MQ=255)
                                                                                     gCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                              <  3:100524/129‑1 (MQ=255)
                                                                                     gCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTAt                                                                              >  4:100524/1‑129 (MQ=255)
                                                                                     gCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtt                                                          >  3:542109/1‑149 (MQ=255)
                                                                                       gACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGAtttt                                                        <  1:550247/149‑1 (MQ=255)
                                                                                           tCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGtt                                                                                <  2:216916/121‑1 (MQ=255)
                                                                                           tCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgcgc                                                    >  1:443787/1‑149 (MQ=255)
                                                                                           tCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGCCTTGCAATAGtt                                                                                >  1:216916/1‑121 (MQ=255)
                                                                                              caTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGt                                                 <  4:443057/149‑1 (MQ=255)
                                                                                              caTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGt                                                 <  2:439772/149‑1 (MQ=255)
                                                                                               aTCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGtt                                                <  4:753582/149‑1 (MQ=255)
                                                                                                  ttCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCa                                             >  1:561910/1‑149 (MQ=255)
                                                                                                    ccgCGATAGTCACATCCACCGTAATCAGGACACCAAAAAACCTGCCGGAGCAAG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATc                                           <  2:561910/147‑1 (MQ=255)
                                                                                                    cAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATc                                           <  3:377378/149‑1 (MQ=255)
                                                                                                       cGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATcgcg                                        >  1:650283/1‑149 (MQ=255)
                                                                                                       cGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATcgcg                                        <  4:147799/149‑1 (MQ=255)
                                                                                                           aGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCAc                                    >  4:47688/1‑149 (MQ=255)
                                                                                                               acaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCg                                >  3:266690/1‑149 (MQ=255)
                                                                                                               acaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCg                                >  1:167347/1‑149 (MQ=255)
                                                                                                               acaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCg                                >  1:259934/1‑149 (MQ=255)
                                                                                                               acaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCg                                >  2:305246/1‑149 (MQ=255)
                                                                                                                 aTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAc                              >  4:442860/1‑149 (MQ=255)
                                                                                                                     aCCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAtt                          <  3:591075/149‑1 (MQ=255)
                                                                                                                      ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAttt                         <  2:574562/149‑1 (MQ=255)
                                                                                                                      ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAttt                         <  2:259934/149‑1 (MQ=255)
                                                                                                                      ccGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAttt                         <  4:704441/149‑1 (MQ=255)
                                                                                                                         tcatcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                      >  2:675454/1‑149 (MQ=255)
                                                                                                                         tcatcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                      <  2:708557/149‑1 (MQ=255)
                                                                                                                         tcatcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGtt                      <  3:142839/149‑1 (MQ=255)
                                                                                                                           atcaGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTtat                    >  2:533867/1‑149 (MQ=255)
                                                                                                                                 acacAAAAAAACCTGCCCGAGCAGG‑TTTTTCGTTATCGGAACATATTGCGTGGCGGTACGTCTTTGAACGTCTTTCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCAGCGCGGCACTCCGACCATTGGTTATACAGgt              >  4:468828/1‑149 (MQ=255)
                                                                                                                                    caAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGtt           <  3:369927/149‑1 (MQ=255)
                                                                                                                                      aaaaaaCCTGCTCCGGCAGGTTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTa          >  3:176694/1‑149 (MQ=255)
                                                                                                                                      aaaaaaCCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt         >  3:751924/1‑149 (MQ=255)
                                                                                                                                           aCCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCt    >  2:462275/1‑149 (MQ=255)
                                                                                                                                             cTGCCGGATCAGG‑TATTTTGTTATCGGAACACATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTCATTTAACATACTTTTGAGGATGTTGCGCAGTTTCGTCGTGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCttt  <  3:468828/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGG‑TTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTT  >  NC_000913/1987712‑1988001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑