Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,999,231 T→A 7.6% T84S (ACT→TCT)  dcyD ← D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,2310TA 7.6% 217.8 / 7.8 67T84S (ACT→TCT) dcyDD‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (26/35);  new base A (3/2);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

TGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAT  >  NC_000913/1999088‑1999378
                                                                                                                                               |                                                                                                                                                   
tGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCTCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGcc                                                                                                                                                <  2:589788/149‑1 (MQ=255)
 gCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCg                                                                                                                                               <  3:748519/149‑1 (MQ=255)
     cGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGcaca                                                                                                                                           <  4:709609/149‑1 (MQ=255)
     cGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGcaca                                                                                                                                           <  2:510934/149‑1 (MQ=255)
      gCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACAt                                                                                                                                          >  1:348299/1‑149 (MQ=255)
      gCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtc                                                                                                                                          <  4:33625/149‑4 (MQ=255)
      gCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGACTGCCGcagtc                                                                                                                                          <  2:135270/149‑4 (MQ=255)
           tttCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTa                                                                                                                                     >  4:230882/1‑149 (MQ=255)
             tCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGa                                                                                                                                   <  4:441877/149‑1 (MQ=255)
              cAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGAc                                                                                                                                  <  4:550046/149‑1 (MQ=255)
              cAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGAc                                                                                                                                  <  3:67113/149‑1 (MQ=255)
                aTCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTg                                                                                                                                >  3:298714/1‑149 (MQ=255)
                    gggTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATc                                                                                                                            <  2:120275/149‑1 (MQ=255)
                      gTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGc                                                                                                                          >  2:98779/1‑149 (MQ=255)
                           gAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCgg                                                                                                                     >  1:563247/1‑149 (MQ=255)
                             aCAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCa                                                                                                                   <  1:615037/149‑1 (MQ=255)
                                 aTCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGa                                                                                                                                   >  2:711512/1‑129 (MQ=255)
                                 aTCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGa                                                                                                                                   <  1:711512/129‑1 (MQ=255)
                                   ccAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATc                                                                                                             <  1:381729/149‑1 (MQ=255)
                                    cAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGGCTGCGGCAGTCGGCCGCACATGGTTAGACTGGATCGCCCCGGCCGTAATCa                                                                                                            <  2:412492/149‑1 (MQ=255)
                                    cAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCa                                                                                                            <  2:90668/149‑1 (MQ=255)
                                    cAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCa                                                                                                            <  2:459413/149‑1 (MQ=255)
                                                aTTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCAc                                                                                                >  2:190902/1‑149 (MQ=255)
                                                     cGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCa                                                                                           >  1:162623/1‑149 (MQ=255)
                                                           ttAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCaga                                                                                     <  2:416009/149‑1 (MQ=255)
                                                            tAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCagag                                                                                    <  3:322060/149‑1 (MQ=255)
                                                             aaaTAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGc                                                                                   >  1:193226/1‑149 (MQ=255)
                                                                tAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATc                                                                                >  2:479817/1‑149 (MQ=255)
                                                                  gTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCATACCGAGTTTGGCAGCGGCTGCGGCAGCCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTg                                                                              >  2:660685/1‑149 (MQ=255)
                                                                      tCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCcgc                                                                          <  1:190902/149‑1 (MQ=255)
                                                                        tGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGTCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCga                                                                        <  2:272989/149‑1 (MQ=255)
                                                                        tGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCga                                                                        <  3:694518/149‑1 (MQ=255)
                                                                        tGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCga                                                                        >  2:627286/1‑149 (MQ=255)
                                                                                 gCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCa                                                               <  2:685720/149‑1 (MQ=255)
                                                                                  ccAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAg                                                              <  3:230882/149‑1 (MQ=255)
                                                                                   cAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGc                                                             >  1:30699/1‑149 (MQ=255)
                                                                                      tAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTa                                                          >  1:253529/1‑149 (MQ=255)
                                                                                        ggATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACg                                                        >  2:572045/1‑149 (MQ=255)
                                                                                         gATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGt                                                       >  1:303322/1‑149 (MQ=255)
                                                                                           ttttCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTaa                                                     >  2:711023/1‑149 (MQ=255)
                                                                                            tttCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAAt                                                    <  1:597588/149‑1 (MQ=255)
                                                                                               ccAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTa                                                 >  2:324065/1‑149 (MQ=255)
                                                                                                 agcagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt                                               >  1:563814/1‑149 (MQ=255)
                                                                                                   cagcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTgc                                             <  2:162623/149‑1 (MQ=255)
                                                                                                    agcGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTgcc                                            <  2:193226/149‑1 (MQ=255)
                                                                                                        ccACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTAc                                                         >  1:243455/1‑132 (MQ=255)
                                                                                                        ccACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTAc                                                         <  2:243455/132‑1 (MQ=255)
                                                                                                         cACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACTCAGAGCATCTGCCGCga                                                                        >  2:637697/1‑116 (MQ=255)
                                                                                                         cACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCga                                                                        <  1:637697/116‑1 (MQ=255)
                                                                                                         cACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCa                                       >  3:551466/1‑149 (MQ=255)
                                                                                                           cGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCAtt                                     <  3:186705/149‑1 (MQ=255)
                                                                                                                   aGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATggg                             <  3:339447/149‑1 (MQ=255)
                                                                                                                     aCCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGATTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt                                               >  3:432382/1‑129 (MQ=255)
                                                                                                                     aCCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTAtt                                               <  4:432382/129‑1 (MQ=255)
                                                                                                                       cGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTg                         >  4:496416/1‑149 (MQ=255)
                                                                                                                        gAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCTGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGa                        <  1:135015/149‑1 (MQ=255)
                                                                                                                         aGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg                            >  1:444146/1‑144 (MQ=255)
                                                                                                                         aGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATgggg                            <  2:444146/144‑1 (MQ=255)
                                                                                                                                  gCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCg              >  4:701043/1‑149 (MQ=255)
                                                                                                                                    gACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGtt            <  2:563247/149‑1 (MQ=255)
                                                                                                                                     aCTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGttt           <  1:408851/149‑1 (MQ=255)
                                                                                                                                      gtGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtg          >  3:417463/2‑149 (MQ=255)
                                                                                                                                       tGCGGCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtga         >  1:674631/1‑149 (MQ=255)
                                                                                                                                        gCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgat        <  3:594031/149‑1 (MQ=255)
                                                                                                                                         cGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgatg       >  3:417139/1‑149 (MQ=255)
                                                                                                                                          ggCAGACTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTtgatga      >  4:502891/1‑149 (MQ=255)
                                                                                                                                              gTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAt  <  2:734160/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGCGTCGCACATTTCAATCTGGGTATTGAACAGATCCAGCAACAAGCGATTGCCGTTGGTTAAATAGTTTTCTGCGGTTGTGCCAATAGGATTTTCCAGCAGCGCCACGCAGTGCAGACCGAGTTTGGCAGCGACTGCGGCAGTCTGCCGCACATGGTTAGACTGGATCGCCCCGGCAGTAATCAGCGTATCGGCACCTTCACGCAGAGCATCTGCCGCGAGAAATTCCAGCTTACGTAATTTATTGCCGCCCATTGCCATGGGGGTGACGTCATCCCGTTTGATGAAAAT  >  NC_000913/1999088‑1999378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑