Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,089,196 | T→A | 6.1% | intergenic (‑68/+15) | yeeZ ← / ← yoeB | putative epimerase/toxin of the YoeB‑YefM toxin‑antitoxin system |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,089,196 | 0 | T | A | 6.1% | 338.9 / 10.2 | 99 | intergenic (‑68/+15) | yeeZ/yoeB | putative epimerase/toxin of the YoeB‑YefM toxin‑antitoxin system |
Reads supporting (aligned to +/- strand): ref base T (47/45); new base A (2/4); total (49/50) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 |
Only 100 of 103 total aligned reads displayed.
GACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTAC > NC_000913/2089048‑2089344 | gACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCt > 2:495932/1‑149 (MQ=255) ttGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTat‑g > 1:508098/1‑149 (MQ=255) ttGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTat‑g > 4:175981/1‑149 (MQ=255) gCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTat‑gaa < 1:595143/149‑1 (MQ=255) gCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTat‑gaa < 2:626206/149‑1 (MQ=255) gCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAt‑gaatgaa < 2:132789/149‑1 (MQ=255) gCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCATa‑gaatgaa < 1:164399/149‑1 (MQ=255) ccTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑gaatgaat < 1:589779/149‑1 (MQ=255) ccTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑gaatgaat < 4:404078/149‑1 (MQ=255) ccTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑gaatgaat < 2:144263/149‑1 (MQ=255) cTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCATA‑gaatgaatt < 3:463025/149‑2 (MQ=255) tCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCt < 1:228401/149‑1 (MQ=255) tCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCt > 4:434748/1‑149 (MQ=255) cgcTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGtt < 2:543833/149‑1 (MQ=255) cgcTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTC‑ATAgaatgaattgtca < 3:113196/149‑6 (MQ=255) gcTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTc < 2:508098/149‑1 (MQ=255) aGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCaataat < 2:609069/149‑1 (MQ=255) gACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGTGACCTTTTTCATCCGTTACCTCCTGATACACCATAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATGTGTTCAATAATGAt > 3:633712/1‑149 (MQ=255) gACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGAt > 2:327999/1‑149 (MQ=255) aCATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATa > 1:426391/1‑149 (MQ=255) aTCGCCAGCGGCATGCCT‑ACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACg > 2:63507/1‑149 (MQ=255) tCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACg > 1:454029/1‑149 (MQ=255) cGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGa < 3:295447/149‑1 (MQ=255) ccAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTat‑gaat < 2:38441/123‑1 (MQ=255) ccAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTat‑gaat > 1:38441/1‑123 (MQ=255) aGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCACCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACAtg < 2:454029/149‑1 (MQ=255) gCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCATA‑gaatgaatt > 2:747376/1‑121 (MQ=255) gCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCATA‑gaatgaatt < 1:747376/122‑2 (MQ=255) aTGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCaa > 1:364158/1‑149 (MQ=255) aaCCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGa > 4:659091/1‑127 (MQ=255) aaCCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGa < 3:659091/127‑1 (MQ=255) aaCCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCa > 3:473983/1‑149 (MQ=255) accaccCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAg < 4:290052/149‑1 (MQ=255) accaccCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAg > 3:753629/1‑149 (MQ=255) accCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCaataat < 4:409981/121‑1 (MQ=255) accCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCaataat > 3:409981/1‑121 (MQ=255) ccTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAt < 1:680961/149‑1 (MQ=255) aCCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAtcgtc < 4:131195/149‑1 (MQ=255) aCCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAtcgtc < 2:85081/149‑1 (MQ=255) cccGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAAgcgtct < 4:516808/149‑2 (MQ=255) cGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGt > 1:319711/1‑149 (MQ=255) gACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTa > 2:35852/1‑149 (MQ=255) cGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGta < 4:472235/149‑1 (MQ=255) gACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGtat > 2:418167/1‑149 (MQ=255) aCCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTAAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGtata > 3:654818/1‑149 (MQ=255) cTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATAcc > 4:676598/1‑149 (MQ=255) tttttCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCa > 2:434801/1‑149 (MQ=255) tCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACg > 1:508814/1‑149 (MQ=255) cATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACgg > 3:605124/1‑149 (MQ=255) tCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTg > 2:202468/1‑149 (MQ=255) cGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGct > 3:700743/1‑149 (MQ=255) tATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGctcct < 1:63507/149‑1 (MQ=255) tcCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGt > 3:218814/1‑149 (MQ=255) tcCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGt > 1:333797/1‑149 (MQ=255) ccTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTa < 1:149038/149‑1 (MQ=255) cTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTaa < 3:536281/149‑1 (MQ=255) cTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTaa > 1:738730/1‑149 (MQ=255) cacCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTc > 1:459087/1‑149 (MQ=255) acCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCg < 4:146521/149‑1 (MQ=255) acCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCg < 2:426391/149‑1 (MQ=255) ccTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCgg > 1:189838/1‑149 (MQ=255) ttAGATCTATAAGGCTACGCTAGCGTATCATAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGa > 3:232430/1‑149 (MQ=255) ttAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCgg > 2:645726/1‑147 (MQ=255) ttAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCgg < 1:645726/147‑1 (MQ=255) tAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGAc < 1:410250/149‑1 (MQ=255) aTCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAg > 1:207456/1‑149 (MQ=255) tataAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGaagccaga > 4:585303/1‑142 (MQ=255) taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAAcc > 1:771634/1‑149 (MQ=255) aaGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCATTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGACACCt < 2:587158/149‑1 (MQ=255) aaGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCt < 1:133450/149‑1 (MQ=255) tACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaa < 4:103151/149‑1 (MQ=255) aCGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACaaa < 1:327999/149‑1 (MQ=255) gCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAAtt > 2:531471/1‑149 (MQ=255) gCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGt > 1:496254/1‑149 (MQ=255) gTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCt < 1:520164/135‑1 (MQ=255) gTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCt > 2:520164/1‑135 (MQ=255) aTCAAAACTGACAATTCATTCTAT‑GAATTAATCTGTTAAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGATTACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATGATGTTTCa > 4:149847/1‑149 (MQ=255) aTCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCa < 1:495932/149‑1 (MQ=255) tCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTc > 2:479853/1‑118 (MQ=255) tCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTc < 1:479853/118‑1 (MQ=255) tCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAg < 4:605124/149‑1 (MQ=255) tCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAg < 2:279458/149‑1 (MQ=255) cAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAgg < 2:319711/149‑1 (MQ=255) cAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAgg < 2:480078/149‑1 (MQ=255) cAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAgg < 1:661096/149‑1 (MQ=255) aaaaCTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAggg > 3:589956/1‑149 (MQ=255) aaaCTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTAt > 2:702685/1‑139 (MQ=255) aaaCTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTAt < 1:702685/139‑1 (MQ=255) aaaCTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAgggg > 2:424607/1‑149 (MQ=255) aaCTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGt > 1:548706/1‑149 (MQ=255) aCTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGtt < 2:189838/149‑1 (MQ=255) cTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTc > 2:127642/1‑149 (MQ=255) tGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCt < 2:207456/149‑1 (MQ=255) aCAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTgg < 2:281607/149‑1 (MQ=255) cAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGc < 3:140814/149‑1 (MQ=255) agattcattcATA‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACcgc < 1:519513/74‑1 (MQ=38) attcattcATA‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGc > 2:590741/1‑147 (MQ=255) tAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTAc < 2:738730/149‑1 (MQ=255) tAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTAc > 1:178879/1‑149 (MQ=255) tAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTAc > 3:164631/1‑149 (MQ=255) | GACTTGCCAGCCTCGCGCTGAAAGTGACATCGCCAGCGGCATGCCTAACCACCCTAACCCGACAATTGCGACCTTTTTCATCCGTTATCTCCTGATACACCTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTAT‑GAATGAATCTGTTCAATAATGATAACGACATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTAC > NC_000913/2089048‑2089344 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |