Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 207,483 A→C 6.4% V786V (GTA→GTC dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913207,4830AC 6.4% 310.0 / 9.8 91V786V (GTA→GTCdnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (43/42);  new base C (3/3);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

GCTGAACTGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTATG  >  NC_000913/207337‑207625
                                                                                                                                                  |                                                                                                                                              
gCTGAACTGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTaac                                                                                                                                              <  3:450515/149‑2 (MQ=255)
gCTGAACTGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAAt                                                                                                                                              >  1:740288/1‑149 (MQ=255)
  tgcaCTGGCGATGAAAATCTTCGACATGGTGGAGAAATTCGCTGGTTACGGATTTCACAAATCGCACTCTGCCGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTACCCTGCGGAGTTTGTGGAGGCGGTCATgt                                                                                                                                            <  2:312951/146‑2 (MQ=255)
  tGAACTGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGa                                                                                                                                            <  1:325154/149‑1 (MQ=255)
           gATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGata                                                                                                                                   >  4:747756/1‑149 (MQ=255)
           gATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGata                                                                                                                                   <  2:418119/149‑1 (MQ=255)
            aTGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGatat                                                                                                                                  <  3:493445/149‑1 (MQ=255)
            aTGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGatat                                                                                                                                  <  1:530725/149‑1 (MQ=255)
             tGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATg                                                                                                                                 <  2:530100/149‑1 (MQ=255)
              gAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATgg                                                                                                                                >  4:570443/1‑149 (MQ=255)
               aaaaTCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTCATTAccgccgccataaa                                                                                                                               <  4:478619/149‑8 (MQ=255)
               aaaaTCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGa                                                                                                                               <  3:301657/149‑1 (MQ=255)
                 aaTCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTaa                                                                                                                                               >  2:630154/1‑131 (MQ=255)
                 aaTCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTaa                                                                                                                                               <  1:630154/131‑1 (MQ=255)
                 aaTCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGaca                                                                                                                             >  4:471890/1‑149 (MQ=255)
                 aaTCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGaca                                                                                                                             >  2:383088/1‑149 (MQ=255)
                   tCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGAACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGacaac                                                                                                                           >  3:542466/1‑149 (MQ=255)
                     ttCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAacac                                                                                                                         >  3:539570/1‑149 (MQ=255)
                            tggtggAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAg                                                                                                                  <  2:318793/149‑1 (MQ=255)
                             ggtggAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAgg                                                                                                                 <  3:576585/149‑1 (MQ=255)
                             ggtggAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAgg                                                                                                                 <  4:303091/149‑1 (MQ=255)
                                ggAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAggtgg                                                                                                              >  4:615808/1‑149 (MQ=255)
                                 gagaAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAggtggt                                                                                                             <  1:415517/149‑1 (MQ=255)
                                 gagaAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAggtggt                                                                                                             <  3:747756/149‑1 (MQ=255)
                                 gagaAATTCGCTGGTTACGGATTTAACAAATCGCACTCAGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAggtggt                                                                                                             <  4:360796/149‑1 (MQ=255)
                                   gaAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGtggtgg                                                                                                           <  1:94095/149‑1 (MQ=255)
                                   gaAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGtggtgg                                                                                                           <  3:470160/149‑1 (MQ=255)
                                   gaAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGtggtgg                                                                                                           <  3:183505/149‑1 (MQ=255)
                                      aTTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTc                                                                                                        <  3:744742/149‑1 (MQ=255)
                                        tCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCtg                                                                                                      <  1:706171/149‑1 (MQ=255)
                                        tCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCtg                                                                                                      >  1:550302/1‑149 (MQ=255)
                                          gCTGGTTACCGATTTAACAAATCGCACTCTGCCGCCTATGCCTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCCGCGGAGTTTATGGCAGCGGTAATGACCGCCGACATGGCCAACCCCGAGAAGGTGGTGGGTCtggt                                                                                                    <  4:650407/149‑1 (MQ=255)
                                           cTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCtggtg                                                                                                   <  2:740288/149‑1 (MQ=255)
                                           cTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCtggtg                                                                                                   >  1:618200/1‑149 (MQ=255)
                                              gTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGAt                                                                                                <  4:299874/149‑1 (MQ=255)
                                               ttACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATg                                                                                               >  1:293606/1‑149 (MQ=255)
                                                tACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGa                                                                                              <  1:432983/149‑1 (MQ=255)
                                                   ggATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTg                                                                                           >  3:747400/1‑149 (MQ=255)
                                                          aCAAATCTCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCgg                                                                                    >  2:412945/1‑149 (MQ=255)
                                                          aCAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGgtggtg                                                                                                            <  2:667240/125‑1 (MQ=255)
                                                          aCAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGgtggtg                                                                                                            >  1:667240/1‑125 (MQ=255)
                                                           cAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGa                                                                                   >  2:558055/1‑149 (MQ=255)
                                                           cAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGa                                                                                   <  2:311059/149‑1 (MQ=255)
                                                            aaaTCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGAt                                                                                  <  3:123882/149‑1 (MQ=255)
                                                            aaaTCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGAt                                                                                  >  4:432603/1‑149 (MQ=255)
                                                               tCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATggg                                                                               <  2:206436/149‑1 (MQ=255)
                                                               tCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATggg                                                                               <  1:646257/149‑1 (MQ=255)
                                                               tCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATggg                                                                               >  1:217440/1‑149 (MQ=255)
                                                                       tGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGaaaa                                                                       <  1:383088/149‑1 (MQ=255)
                                                                       tGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGaaaa                                                                       >  4:235699/1‑149 (MQ=255)
                                                                        gCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGGCCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAAt                                                                      <  2:609632/149‑1 (MQ=255)
                                                                              tATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGcc                                                                <  3:732644/149‑1 (MQ=255)
                                                                              tATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGcc                                                                >  1:187277/1‑149 (MQ=255)
                                                                               aTGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGCCAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGcca                                                               <  4:409230/149‑1 (MQ=255)
                                                                                  cTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGccacca                                                            >  3:173311/1‑149 (MQ=255)
                                                                                   tttGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTCATTAccgccg                                                                                                                                      <  4:708752/74‑1 (MQ=255)
                                                                                   tttGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTCATTAccgccg                                                                                                                                      >  3:708752/1‑74 (MQ=255)
                                                                                   tttGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAg                                                           >  2:665837/1‑149 (MQ=255)
                                                                                    ttGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGa                                                          <  1:343068/149‑1 (MQ=255)
                                                                                          tCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATaaa                                                    <  2:293606/149‑1 (MQ=255)
                                                                                            atatCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACt                                                  >  1:120448/1‑149 (MQ=255)
                                                                                              atCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTcc                                                >  1:426682/1‑149 (MQ=255)
                                                                                               tCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGa                                                                                              <  4:180398/102‑1 (MQ=255)
                                                                                               tCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGa                                                                                              >  3:180398/1‑102 (MQ=255)
                                                                                               tCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCg                                               >  3:172378/1‑149 (MQ=255)
                                                                                               tCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCg                                               >  4:587606/1‑149 (MQ=255)
                                                                                               tCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCg                                               >  2:466762/1‑149 (MQ=255)
                                                                                                cAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCgg                                              >  3:295890/1‑149 (MQ=255)
                                                                                                 aaaCGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGt                                             >  1:254422/1‑149 (MQ=255)
                                                                                                 aaaCGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGt                                             >  2:696834/1‑149 (MQ=255)
                                                                                                 aaaCGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGt                                             >  3:331336/1‑149 (MQ=255)
                                                                                                     gTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCttt                                         <  4:542466/149‑1 (MQ=255)
                                                                                                     gTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCttt                                         >  4:8119/1‑149 (MQ=255)
                                                                                                          ggCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCt                                                                   >  1:273607/1‑118 (MQ=255)
                                                                                                          ggCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCt                                                                   <  2:273607/118‑1 (MQ=255)
                                                                                                          ggCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCAt                                    >  1:392232/1‑149 (MQ=255)
                                                                                                          ggCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCAt                                    >  1:688021/1‑149 (MQ=255)
                                                                                                              gAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTcc                                >  2:365565/1‑149 (MQ=255)
                                                                                                              gAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTcc                                <  2:187277/149‑1 (MQ=255)
                                                                                                               aaaGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGa                                                                                                 >  3:696369/1‑83 (MQ=255)
                                                                                                               aaaGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGa                                                                                                 <  4:696369/83‑1 (MQ=255)
                                                                                                                aaGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCAc                              >  1:592432/1‑149 (MQ=255)
                                                                                                                  gcgcACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGt                            >  3:265472/1‑149 (MQ=255)
                                                                                                                    gcACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCa                          >  4:571417/1‑149 (MQ=255)
                                                                                                                      aCTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAac                        <  3:583267/149‑1 (MQ=255)
                                                                                                                             tGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAacgacgacg                 >  3:314981/1‑149 (MQ=255)
                                                                                                                             tGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGCGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAacgacgacg                 >  2:66698/1‑149 (MQ=255)
                                                                                                                                ggAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCg              >  3:83920/1‑149 (MQ=255)
                                                                                                                                       atatcggcggTCATTACCGCCGATATGGACAACACCGAGAAGGAGGTGGGTCTGGTGGATGACTGCTGGCGGATGGTGCTGAAAATCCTGCCACCAGATATAAACTCAGGTCTTTACTATTTCCACGTCAACGACGACGGCGAAATCgt       >  3:468328/5‑149 (MQ=255)
                                                                                                                                       aTGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCgt       <  3:587606/149‑1 (MQ=255)
                                                                                                                                         ggcggcGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCgtgt     <  2:592432/149‑1 (MQ=255)
                                                                                                                                          gcggcgGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTa    <  3:8119/149‑1 (MQ=255)
                                                                                                                                           cggcggTCATTACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTAt   >  2:208954/1‑149 (MQ=255)
                                                                                                                                            ggcggTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTATg  <  4:173311/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GCTGAACTGGCGATGAAAATCTTCGACCTGGTGGAGAAATTCGCTGGTTACGGATTTAACAAATCGCACTCTGCGGCCTATGCTTTGGTGTCATATCAAACGTTATGGCTGAAAGCGCACTATCCTGCGGAGTTTATGGCGGCGGTAATGACCGCCGATATGGACAACACCGAGAAGGTGGTGGGTCTGGTGGATGAGTGCTGGCGGATGGGGCTGAAAATCCTGCCACCAGATATAAACTCCGGTCTTTACCATTTCCACGTCAACGACGACGGCGAAATCGTGTATG  >  NC_000913/207337‑207625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: