Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,434,097 T→C 5.7% A215A (GCA→GCG dedA ← DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,434,0970TC 5.7% 338.0 / 7.5 105A215A (GCA→GCGdedADedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (41/58);  new base C (3/3);  total (44/61)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 109 total aligned reads displayed.

TGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTA  >  NC_000913/2433949‑2434239
                                                                                                                                                    |                                                                                                                                              
tGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGt                                                                                                                                                >  3:371710/1‑149 (MQ=255)
tGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGt                                                                                                                                                <  1:69419/149‑1 (MQ=255)
tGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGt                                                                                                                                                >  4:373570/1‑149 (MQ=255)
        tGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAgc                                                                                                                                        <  3:757313/149‑1 (MQ=255)
         gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAgcg                                                                                                                                       >  3:450101/1‑149 (MQ=255)
         gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                                                                       <  1:165353/149‑4 (MQ=255)
         gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                                                                       <  2:460839/149‑4 (MQ=255)
            aGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGt                                                                                                                                    >  3:144526/1‑149 (MQ=255)
              ttttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGttt                                                                                                                                  >  1:619021/1‑149 (MQ=255)
              ttttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGttt                                                                                                                                  <  1:329649/149‑1 (MQ=255)
                  aTCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGa                                                                                                                              <  2:98320/149‑1 (MQ=255)
                   tCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGAc                                                                                                                             >  2:746367/1‑149 (MQ=255)
                   tCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGAc                                                                                                                             <  3:701375/149‑1 (MQ=255)
                      ttGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGa                                                                                                                          <  1:560113/149‑1 (MQ=255)
                        gggtACGCATAATGCCATTTTTGCCCCCAACATACAATTAATGTTGAACATTCAACCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGATCCGTGGAGTTACTTTTTATTTTGCGGCGTGTGCCGCAGCGCGTTTGTGTCGGatt                                                                                                                        <  2:563425/149‑1 (MQ=255)
                         gggACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGatta                                                                                                                       <  3:720966/149‑1 (MQ=255)
                           gACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGattatt                                                                                                                     <  3:468207/149‑1 (MQ=255)
                             cGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGTGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTc                                                                                                                   <  4:278209/149‑1 (MQ=255)
                             cGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTc                                                                                                                   <  1:38037/149‑1 (MQ=255)
                               cATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGa                                                                                                                 <  1:26328/149‑1 (MQ=255)
                                aTAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGAt                                                                                                                <  1:401733/149‑1 (MQ=255)
                                aTAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGAt                                                                                                                <  2:457308/149‑1 (MQ=255)
                                   aTGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGAc                                                                                                             >  1:481479/1‑149 (MQ=255)
                                     gCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGc                                                                                                           >  3:579277/1‑149 (MQ=255)
                                        aTTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCg                                                                                                        <  4:451289/149‑1 (MQ=255)
                                              gCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCaaaa                                                                                                  <  3:618970/149‑1 (MQ=255)
                                              gCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCaaaa                                                                                                  <  3:14057/149‑1 (MQ=255)
                                                ccccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATa                                                                                                >  1:337486/1‑149 (MQ=255)
                                                ccccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATa                                                                                                <  1:230535/149‑1 (MQ=255)
                                                 cccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAg                                                                                               <  2:427205/149‑1 (MQ=255)
                                                 cccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAg                                                                                               >  1:92355/1‑149 (MQ=255)
                                                  ccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGa                                                                                              <  1:510134/149‑1 (MQ=255)
                                                  ccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGa                                                                                              >  2:363925/1‑149 (MQ=255)
                                                   cAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGTACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTCCGATGACGCCCGGCAAAATAGaa                                                                                             >  4:712036/1‑149 (MQ=255)
                                                   cAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGaa                                                                                             <  4:164828/149‑1 (MQ=255)
                                                    aaCAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                                                                       <  4:506833/106‑4 (MQ=255)
                                                    aaCAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaa                                                                                                                                       >  3:506833/1‑103 (MQ=255)
                                                          ccATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaa                                                                                      <  1:363925/149‑1 (MQ=255)
                                                           cATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaat                                                                                     >  3:307413/1‑149 (MQ=255)
                                                           cATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaat                                                                                     >  4:577831/1‑149 (MQ=255)
                                                            aTGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGCCGCCCGGCAAAATAGAAACCACaata                                                                                    >  1:251618/1‑149 (MQ=255)
                                                            aTGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaata                                                                                    >  1:698377/1‑149 (MQ=255)
                                                            aTGAATGTTGCACAATAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaata                                                                                    >  3:499585/1‑149 (MQ=255)
                                                             tGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtgt                                                                                                                                <  1:83866/104‑1 (MQ=255)
                                                             tGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtgt                                                                                                                                >  2:83866/1‑104 (MQ=255)
                                                              gAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaataat                                                                                  >  2:323600/1‑149 (MQ=255)
                                                                 tGTTGCACATTAAAACATAACAGGCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATccc                                                                               >  2:107665/1‑149 (MQ=255)
                                                                    tGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgac                                                                            <  2:619021/149‑1 (MQ=255)
                                                                    tGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgac                                                                            <  1:457283/149‑1 (MQ=255)
                                                                    tGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgac                                                                            <  3:242963/149‑1 (MQ=255)
                                                                     gCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcga                                                                             <  1:112463/147‑1 (MQ=255)
                                                                     gCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcga                                                                             >  2:112463/1‑147 (MQ=255)
                                                                       acaTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGAt                                                                         >  2:188326/1‑149 (MQ=255)
                                                                        caTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATc                                                                        <  4:579277/149‑1 (MQ=255)
                                                                         aTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATca                                                                       >  3:211071/1‑149 (MQ=255)
                                                                         aTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATca                                                                       >  4:654579/1‑149 (MQ=255)
                                                                         aTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATca                                                                       >  4:163673/1‑149 (MQ=255)
                                                                          ttAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATcag                                                                      >  3:628069/1‑149 (MQ=255)
                                                                          ttAAAACATAACAGCACGAAACTTTGGATAAAAAAGTGGTCGTACCGAAGAGATACTTTTTATTTTGCGGCGCGTGCGGCAGCGCGGCTGTGACGGATTATTTCGATCTCGCTCGGCAAAATAGAAACCAGAATAATCGcgacgagccg                                                                      >  2:760081/1‑145 (MQ=255)
                                                                             aaaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGTGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATa                                                                                                <  2:228809/120‑1 (MQ=255)
                                                                             aaaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATa                                                                                                >  1:228809/1‑120 (MQ=255)
                                                                                cATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCtt                                                                >  3:69397/1‑149 (MQ=255)
                                                                                  tAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTaa                                                              <  2:375295/149‑1 (MQ=255)
                                                                                  tAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTaa                                                              <  4:57514/149‑1 (MQ=255)
                                                                                        cccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTACGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTAt                                                        <  3:646665/149‑1 (MQ=255)
                                                                                         ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATc                                                       <  2:510056/149‑1 (MQ=255)
                                                                                         ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATc                                                       <  1:190477/149‑1 (MQ=255)
                                                                                         ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATc                                                       <  2:698377/149‑1 (MQ=255)
                                                                                         ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATc                                                       >  1:534130/1‑149 (MQ=255)
                                                                                                tttGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCaaaa                                                                                                  >  4:680052/1‑99 (MQ=255)
                                                                                                tttGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCaaaa                                                                                                  <  3:680052/99‑1 (MQ=255)
                                                                                                     aTAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCgg                                           >  2:480061/1‑149 (MQ=255)
                                                                                                       aaaaaaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGa                                         <  4:324869/149‑1 (MQ=255)
                                                                                                         aaaaGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGAtt                                       <  2:481479/149‑1 (MQ=255)
                                                                                                            aGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTa                                    <  2:337486/149‑1 (MQ=255)
                                                                                                                 tCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaa                               <  4:211071/149‑1 (MQ=255)
                                                                                                                 tCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaa                               <  3:539586/149‑1 (MQ=255)
                                                                                                                       cgcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATa                         >  2:522430/1‑149 (MQ=255)
                                                                                                                        gcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAg                        <  4:661855/149‑1 (MQ=255)
                                                                                                                         cgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGc                       <  3:73130/149‑1 (MQ=255)
                                                                                                                              ttACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCg                  >  3:346083/1‑149 (MQ=255)
                                                                                                                                 cTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAg               <  4:304624/149‑1 (MQ=255)
                                                                                                                                  tttttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGTATTGTACCGAAGAAATAGCCTGCGTAgg              >  1:113245/1‑149 (MQ=255)
                                                                                                                                  tttttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAgg              >  2:702916/1‑149 (MQ=255)
                                                                                                                                  tttttATTTTCCGGCGCGTGCCGCACCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGATTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAgg              >  1:410539/1‑149 (MQ=255)
                                                                                                                                   ttttATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGt             >  3:285157/1‑149 (MQ=255)
                                                                                                                                        ttttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaaa        <  4:733121/149‑1 (MQ=255)
                                                                                                                                        ttttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaaa        >  4:437050/1‑149 (MQ=255)
                                                                                                                                        ttttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTaaaaa        <  3:427159/149‑1 (MQ=255)
                                                                                                                                          ttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGaa                                                  >  3:166378/1‑105 (MQ=255)
                                                                                                                                          ttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGaa                                                  <  4:166378/105‑1 (MQ=255)
                                                                                                                                          ctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGc      >  2:538240/2‑149 (MQ=255)
                                                                                                                                           tGCGGCGTGTGCCGCAGCGCGATTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCa     <  2:251618/149‑1 (MQ=255)
                                                                                                                                           tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAACATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCa     <  3:654579/149‑1 (MQ=255)
                                                                                                                                           tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCa     <  4:628069/149‑1 (MQ=255)
                                                                                                                                           tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCa     <  2:159028/149‑1 (MQ=255)
                                                                                                                                           tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCa     <  2:113245/149‑1 (MQ=255)
                                                                                                                                           tGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCa     <  1:316188/149‑1 (MQ=255)
                                                                                                                                             cggcgCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGt   <  3:327744/149‑1 (MQ=255)
                                                                                                                                              ggcgCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTa  <  4:371710/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTA  >  NC_000913/2433949‑2434239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: