Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,549,556 C→G 5.2% intergenic (+150/+90) yfeW → / ← yfeX penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,549,5560CG 5.2% 269.1 / 5.1 78intergenic (+150/+90)yfeW/yfeXpenicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic
Reads supporting (aligned to +/- strand):  ref base C (33/40);  new base G (2/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

AAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACC  >  NC_000913/2549408‑2549700
                                                                                                                                                    |                                                                                                                                                
aaaaaGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                                  >  3:479524/1‑149 (MQ=255)
aaaaaGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATAGATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                                  >  1:226669/1‑149 (MQ=255)
   aaGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                                                               >  4:645341/1‑149 (MQ=255)
        cagtcagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                          <  1:6026/149‑2 (MQ=25)
        cagtcagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCg                                                                                                                                          >  1:369846/1‑149 (MQ=255)
         agtcagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCgt                                                                                                                                         <  3:44366/149‑1 (MQ=255)
           tcagtAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGGATGCGGCgtga                                                                                                                                       <  3:634994/149‑2 (MQ=255)
               tAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACaa                                                                                                                                   >  2:712212/1‑149 (MQ=255)
                  tGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAAcgc                                                                                                                                <  3:322440/149‑1 (MQ=255)
                   gTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCACTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAAcgcg                                                                                                                               >  1:505806/1‑149 (MQ=255)
                    ttACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACgcgc                                                                                                                              >  4:563864/1‑149 (MQ=255)
                        tggctggTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACtt                                                                                                                          <  3:513258/149‑1 (MQ=255)
                        tggctggTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACtt                                                                                                                          >  3:10146/1‑149 (MQ=255)
                          gctggTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTgg                                                                                                                        >  3:709403/1‑149 (MQ=255)
                           ctggTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGt                                                                                                                       <  4:576306/149‑1 (MQ=255)
                                      gTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGa                                                                                                            <  3:419258/149‑1 (MQ=255)
                                       tGCCGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAg                                                                                                           <  1:379153/149‑1 (MQ=255)
                                        gCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAgg                                                                                                          <  1:15556/149‑1 (MQ=255)
                                          tGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGcc                                                                                                        >  4:575095/1‑149 (MQ=255)
                                               aaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcagtca                                                                                                   >  1:306936/1‑149 (MQ=255)
                                                  gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTa                                                                                                >  2:542765/1‑149 (MQ=255)
                                                       aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGtt                                                                                           <  3:139720/149‑1 (MQ=255)
                                                        cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTa                                                                                          >  3:11991/1‑149 (MQ=255)
                                                             tGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGcc                                                                                     >  4:263283/1‑149 (MQ=255)
                                                               aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTactgactg                                                                                   <  2:753671/149‑1 (MQ=255)
                                                                  cgcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGcc                                                                                <  3:733998/149‑1 (MQ=255)
                                                                   gcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCt                                                                               <  3:644393/149‑1 (MQ=255)
                                                                   gcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCt                                                                               >  2:660648/1‑149 (MQ=255)
                                                                    cTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCtt                                                                              <  2:226669/149‑1 (MQ=255)
                                                                        gCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAAt                                                                          <  2:369846/149‑1 (MQ=255)
                                                                         cTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATc                                                                         >  4:176846/1‑149 (MQ=255)
                                                                           tATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa                                                                       <  3:688109/149‑1 (MQ=255)
                                                                           tATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa                                                                       <  3:421898/149‑1 (MQ=255)
                                                                             tCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATg                                                                     >  3:632752/1‑149 (MQ=255)
                                                                               aGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGaa                                                                   <  1:236655/149‑1 (MQ=255)
                                                                                ggCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAAt                                                                  <  4:595400/149‑1 (MQ=255)
                                                                                ggCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAAt                                                                  <  4:604977/149‑1 (MQ=255)
                                                                                 gCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcag                                                                                                      <  1:118696/112‑1 (MQ=255)
                                                                                 gCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcag                                                                                                      >  2:118696/1‑112 (MQ=255)
                                                                                   cTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGTTAAGGCGTTCACGCCGCATCCGGCGCATACAACGCGCACCCGGTCAACAATCTGAGTCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAg                                                               >  4:496414/1‑149 (MQ=37)
                                                                                        tGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACg                                                          >  1:541882/1‑149 (MQ=255)
                                                                                          gAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCt                                                        <  4:253233/149‑1 (MQ=255)
                                                                                            gCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa                                                                       <  2:708262/132‑1 (MQ=255)
                                                                                            gCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCaa                                                                       >  1:708262/1‑132 (MQ=255)
                                                                                            gCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTa                                                      <  3:575095/149‑1 (MQ=255)
                                                                                            gCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTa                                                      <  4:694215/149‑1 (MQ=255)
                                                                                            gCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTa                                                      <  2:32440/149‑1 (MQ=255)
                                                                                              ccACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACa                                                    <  2:214538/149‑1 (MQ=255)
                                                                                               cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAg                                                   >  2:716208/1‑149 (MQ=255)
                                                                                               cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAg                                                   >  4:601603/1‑149 (MQ=255)
                                                                                               cacaATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAg                                                   >  1:179627/1‑149 (MQ=255)
                                                                                                   atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAgc                                                  >  1:731624/1‑146 (MQ=255)
                                                                                                   atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAgc                                                  <  2:731624/146‑1 (MQ=255)
                                                                                                   atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGcc                                               >  1:609066/1‑149 (MQ=255)
                                                                                                    tatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCa                                              <  4:479524/149‑1 (MQ=255)
                                                                                                       atTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCa                                           >  3:395785/1‑149 (MQ=255)
                                                                                                        ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcagtc                                                                                                    <  1:348069/91‑1 (MQ=255)
                                                                                                        ttGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCcagtc                                                                                                    >  2:348069/1‑91 (MQ=255)
                                                                                                         tGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAAc                                         <  3:563864/149‑1 (MQ=255)
                                                                                                           aaTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACtt                                       <  4:632752/149‑1 (MQ=255)
                                                                                                              tAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTc                                    <  2:505806/149‑1 (MQ=255)
                                                                                                              tAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTc                                    <  4:10146/149‑1 (MQ=255)
                                                                                                               aGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTcc                                   <  2:716823/149‑1 (MQ=255)
                                                                                                                gCACGATTTCGCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCa                                  >  4:457415/1‑149 (MQ=255)
                                                                                                                 cACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAg                                 <  1:557705/149‑1 (MQ=255)
                                                                                                                 cACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAg                                 >  2:415752/1‑149 (MQ=255)
                                                                                                                     aTTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGAc                             <  2:728793/149‑1 (MQ=255)
                                                                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGaaa                    >  1:682110/1‑149 (MQ=255)
                                                                                                                                cGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTGGcc                                                                                >  2:176815/1‑87 (MQ=21)
                                                                                                                                cGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTGGcc                                                                                <  1:176815/87‑1 (MQ=21)
                                                                                                                                  gATAAGGCGTTCCCGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAaataat                <  4:577407/149‑1 (MQ=255)
                                                                                                                                   aTAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAataata               <  2:306936/149‑1 (MQ=255)
                                                                                                                                    tAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAg              <  3:293978/149‑1 (MQ=255)
                                                                                                                                       ggCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGaaa                    <  3:326087/140‑1 (MQ=255)
                                                                                                                                       ggCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGaaa                    >  4:326087/1‑140 (MQ=255)
                                                                                                                                        ttgtacACGCCGGATGCGGCGTGAACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgccgc          >  2:159599/6‑149 (MQ=255)
                                                                                                                                          gTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGccgccg        >  3:160276/1‑149 (MQ=255)
                                                                                                                                           ttCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCgg       >  1:296155/1‑149 (MQ=255)
                                                                                                                                           tacACGCCGGATGCGGCGTGAACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAaataat                <  4:193080/138‑1 (MQ=255)
                                                                                                                                           tacACGCCGGATGCGGCGTGAACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAaataat                >  3:193080/3‑140 (MQ=255)
                                                                                                                                               cgccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTAc   <  4:269657/149‑1 (MQ=255)
                                                                                                                                                gccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTAcc  >  1:21879/1‑149 (MQ=255)
                                                                                                                                                gccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTAcc  >  3:232892/1‑149 (MQ=255)
                                                                                                                                                   gcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTAcc  <  2:276589/146‑1 (MQ=255)
                                                                                                                                                   gcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTAcc  >  1:276589/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AAAAAGGCCAGTCAGTAATGTTACTGGCTGGTCTGAGAGTGCTGACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACC  >  NC_000913/2549408‑2549700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: