Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,566,266 C→A 7.8% G207C (GGC→TGC)  eutA ← reactivating factor for ethanolamine ammonia lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,566,2660CA 7.8% 267.4 / 10.7 77G207C (GGC→TGC) eutAreactivating factor for ethanolamine ammonia lyase
Reads supporting (aligned to +/- strand):  ref base C (34/37);  new base A (3/3);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

GCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCG  >  NC_000913/2566119‑2566414
                                                                                                                                                   |                                                                                                                                                    
gCGTCACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCt                                                                                                                                                     <  2:59911/149‑1 (MQ=255)
gCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCt                                                                                                                                                     <  4:376597/149‑1 (MQ=255)
gCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCt                                                                                                                                                     >  1:450621/1‑149 (MQ=255)
gCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCt                                                                                                                                                     <  1:360527/149‑1 (MQ=255)
  gTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGc                                                                                                                                                   >  1:323731/1‑149 (MQ=255)
        ccTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGaa                                                                                                                                             <  4:543327/149‑1 (MQ=255)
         cTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAg                                                                                                                                            >  1:130234/1‑149 (MQ=255)
          tGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGc                                                                                                                                           <  2:585262/149‑1 (MQ=255)
             gggCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACt                                                                                                                                        >  1:90080/1‑149 (MQ=255)
             gggCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACt                                                                                                                                        <  1:474/149‑1 (MQ=255)
                    aaaCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCcac                                                                                                                                 <  4:699428/149‑1 (MQ=255)
                     aaCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGac                                                                                                                                             >  2:67817/1‑135 (MQ=255)
                     aaCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGac                                                                                                                                             <  1:67817/136‑2 (MQ=255)
                           tttGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatcatc                                                                                                                          >  4:502490/1‑149 (MQ=255)
                                 tCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGcccc                                                                                                                    >  3:640866/1‑149 (MQ=255)
                                 tCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGcccc                                                                                                                    >  3:737962/1‑149 (MQ=255)
                                 tCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGac                                                                                                                                             <  4:552714/124‑2 (MQ=255)
                                 tCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGac                                                                                                                                             >  3:552714/1‑123 (MQ=255)
                                  cAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCg                                                                                                                   <  1:138032/149‑1 (MQ=255)
                                       ccTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTa                                                                                                              <  4:622116/149‑1 (MQ=255)
                                        cTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCCTCTGCCCCGGTTTAt                                                                                                             <  2:124699/149‑1 (MQ=255)
                                        cTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTAt                                                                                                             >  1:239602/1‑149 (MQ=255)
                                          gcgcGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGa                                                                                                           <  1:73030/149‑1 (MQ=255)
                                            gcgAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGc                                                                                                         >  1:568718/1‑149 (MQ=255)
                                                 cGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    <  3:705654/149‑1 (MQ=255)
                                                  ggTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAAc                                                                                                   >  3:22396/1‑149 (MQ=255)
                                                   gTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAAcc                                                                                                  >  1:586259/1‑149 (MQ=255)
                                                    tGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 >  1:283429/1‑149 (MQ=255)
                                                            ttCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCg                                                                                         >  2:39530/1‑149 (MQ=255)
                                                                   aaTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGt                                                                                  >  1:690090/1‑149 (MQ=255)
                                                                    aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTc                                                                                 <  4:398777/149‑1 (MQ=255)
                                                                    aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTc                                                                                 <  2:357538/149‑1 (MQ=255)
                                                                       aCTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGt                                                                              >  3:320611/1‑149 (MQ=255)
                                                                        cTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGtt                                                                             <  4:259872/149‑1 (MQ=255)
                                                                         ttCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTg                                                                                       >  2:538298/1‑138 (MQ=255)
                                                                         ttCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTg                                                                                       <  1:538298/138‑1 (MQ=255)
                                                                         ttCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGttt                                                                            >  2:142686/1‑149 (MQ=255)
                                                                          tCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTc                                                                           <  2:772870/149‑1 (MQ=255)
                                                                           cGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTcc                                                                          <  4:27799/149‑1 (MQ=255)
                                                                                aTCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagcag                                                                     >  4:544601/1‑149 (MQ=255)
                                                                                 tCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAgg                                                                    >  2:97263/1‑149 (MQ=255)
                                                                                    gTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGa                                                                 <  4:94731/149‑1 (MQ=255)
                                                                                     ttCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGac                                                                >  4:186086/1‑149 (MQ=255)
                                                                                       cTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGacca                                                              <  2:568718/149‑1 (MQ=255)
                                                                                        tGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGaccac                                                             <  2:90080/149‑1 (MQ=255)
                                                                                        tGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGaccac                                                             >  2:80863/1‑149 (MQ=255)
                                                                                             tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACg                                                        >  3:379027/1‑149 (MQ=255)
                                                                                                      tCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggcag                                               >  1:16587/1‑149 (MQ=255)
                                                                                                      tCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggcag                                               <  2:710668/149‑1 (MQ=255)
                                                                                                      tCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggcag                                               <  2:283429/149‑1 (MQ=255)
                                                                                                       cACCTGCACCAGCTCCCCGCCGGTCAGCGAACGGGGGTCGATGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggcagg                                              >  4:722861/1‑149 (MQ=255)
                                                                                                           tGCACCATCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGt                                          >  2:422760/1‑149 (MQ=255)
                                                                                                              aCCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGcc                                       <  4:198898/149‑1 (MQ=255)
                                                                                                               ccAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGcc                                       >  3:167886/1‑148 (MQ=255)
                                                                                                               ccAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGcc                                       <  4:167886/148‑1 (MQ=255)
                                                                                                                 aGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCt                                    <  3:470491/149‑1 (MQ=255)
                                                                                                                    tGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGAt                                 <  2:16587/149‑1 (MQ=255)
                                                                                                                       gcgcCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGAtttt                              >  4:546334/1‑149 (MQ=255)
                                                                                                                          ccGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTTGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTcc                           <  4:737962/149‑1 (MQ=255)
                                                                                                                          ccGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTcc                           <  3:628631/149‑1 (MQ=255)
                                                                                                                                aGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATc                     >  1:189598/1‑149 (MQ=255)
                                                                                                                                 gCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCg                    >  3:73639/1‑149 (MQ=255)
                                                                                                                                   gAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGaa                  >  3:607482/1‑149 (MQ=255)
                                                                                                                                     aCGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACa                <  4:607482/149‑1 (MQ=255)
                                                                                                                                       gggCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAgg              <  2:189598/149‑1 (MQ=255)
                                                                                                                                       gggCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAgg              <  2:444702/149‑1 (MQ=255)
                                                                                                                                        ggCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCg                          >  3:692039/1‑136 (MQ=255)
                                                                                                                                        ggCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCg                          <  4:692039/136‑1 (MQ=255)
                                                                                                                                         gCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGc            >  1:29841/1‑149 (MQ=255)
                                                                                                                                         gCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGc            >  3:712859/1‑149 (MQ=255)
                                                                                                                                         gCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGc            <  2:91778/149‑1 (MQ=255)
                                                                                                                                           ttCGGTGCAGGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGt          >  1:278348/2‑149 (MQ=255)
                                                                                                                                             cGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAg        <  4:712859/149‑1 (MQ=255)
                                                                                                                                             cGGTGCAGGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAg        <  2:278348/149‑1 (MQ=255)
                                                                                                                                              ggTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGt       <  4:320611/149‑1 (MQ=255)
                                                                                                                                               gTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggcagg                                              >  2:433421/1‑109 (MQ=255)
                                                                                                                                               gTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggcagg                                              <  1:433421/109‑1 (MQ=255)
                                                                                                                                                   cTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTcgcg  >  1:140318/1‑149 (MQ=255)
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GCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCG  >  NC_000913/2566119‑2566414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: