Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,873,915 A→G 7.1% H26H (CAT→CAC) ‡ cysC ← adenosine 5'‑phosphosulfate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,873,9150AG 7.1% 218.3 / 5.8 70H26H (CAT→CAC) ‡cysCadenosine 5'‑phosphosulfate kinase
Reads supporting (aligned to +/- strand):  ref base A (27/38);  new base G (3/2);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAT  >  NC_000913/2873768‑2874058
                                                                                                                                                   |                                                                                                                                               
gagaTCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGAt                                                                                                                                                <  1:335460/149‑1 (MQ=255)
   aTCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGAc                                                                                                                                             >  1:55733/1‑149 (MQ=255)
    tCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGAcc                                                                                                                                            <  4:352024/149‑1 (MQ=255)
         gCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggt                                                                                                                                       <  3:403648/149‑1 (MQ=255)
          cATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggtg                                                                                                                                      <  4:662433/149‑1 (MQ=255)
           aTAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGgtgt                                                                                                                                     <  3:153245/149‑1 (MQ=255)
            tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                    <  1:307339/149‑1 (MQ=255)
            tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                    >  2:142781/1‑149 (MQ=255)
              aTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGc                                                                                                                                  <  1:137411/149‑1 (MQ=255)
                    ggCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCAGAGCACTACACCACGATGACCGTGGTGTAGCTCGCgt                                                                                                                            <  4:718080/149‑1 (MQ=255)
                    ggCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgt                                                                                                                            <  1:570615/149‑1 (MQ=255)
                       gAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                         >  1:559377/1‑149 (MQ=255)
                       gAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                         <  2:697968/149‑1 (MQ=255)
                         aCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGttgttg                                                                                                                       <  1:269053/149‑1 (MQ=255)
                                       cagcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                         <  1:642441/149‑1 (MQ=255)
                                        agcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATg                                                                                                        >  2:691205/1‑149 (MQ=255)
                                         gcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATgg                                                                                                       <  4:265563/149‑1 (MQ=255)
                                            gATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTa                                                                                                    >  1:383394/1‑149 (MQ=255)
                                                       aCGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgacgac                                                                                         <  1:146872/149‑1 (MQ=255)
                                                         gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                    <  4:655345/104‑1 (MQ=255)
                                                         gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCAGCGTGGTGTa                                                                                                                                    >  3:655345/1‑104 (MQ=255)
                                                         gCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGt                                                                                       >  4:40731/1‑149 (MQ=255)
                                                          ccGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGtt                                                                                      >  2:672867/1‑149 (MQ=255)
                                                            gAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGtttt                                                                                    >  2:318795/1‑149 (MQ=255)
                                                             aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                   <  4:206148/149‑1 (MQ=255)
                                                              gTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCg                                                                                  <  1:318795/149‑1 (MQ=255)
                                                                ttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTc                                                                                <  2:451731/149‑1 (MQ=255)
                                                                  aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCAt                                                                              <  2:431072/149‑1 (MQ=255)
                                                                  aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCAt                                                                              <  2:674893/149‑1 (MQ=255)
                                                                    gTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCt                                                                                                     <  4:108182/124‑1 (MQ=255)
                                                                    gTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCt                                                                                                     >  3:108182/1‑124 (MQ=255)
                                                                    gTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGc                                                                                                 <  3:245422/128‑1 (MQ=255)
                                                                    gTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGc                                                                                                 >  4:245422/1‑128 (MQ=255)
                                                                    gTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGc                                                                            <  2:233461/149‑1 (MQ=255)
                                                                     tAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCa                                                                           >  2:720708/1‑149 (MQ=255)
                                                                      aaCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAg                                                                          >  2:526229/1‑149 (MQ=255)
                                                                              ctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGACGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCatta                                                                  >  3:184136/1‑149 (MQ=255)
                                                                                   gcgcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCAttatttat                                                             <  4:754109/149‑1 (MQ=255)
                                                                                   gcgcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCAttatttat                                                             <  2:295194/149‑1 (MQ=255)
                                                                                    cgcCCCGGCTACCGTTGATTTACGGGACACGGAGAGGCCGGTAAACCACCGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTGCGTCATGCAGCGCCATTATTTATc                                                            <  3:639922/149‑1 (MQ=255)
                                                                                      ccccGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATccc                                                          >  3:681876/1‑149 (MQ=255)
                                                                                              aCCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCaaa                                                  <  4:715902/149‑1 (MQ=255)
                                                                                              aCCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCaaa                                                  <  1:467293/149‑1 (MQ=255)
                                                                                              aCCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCaaa                                                  <  3:316894/149‑1 (MQ=255)
                                                                                                 gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcg                                               >  2:743425/1‑149 (MQ=255)
                                                                                                  ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgc                                              <  2:559377/149‑1 (MQ=255)
                                                                                                  ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgc                                              >  3:724283/1‑149 (MQ=255)
                                                                                                          ccGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATc                                                >  2:364952/1‑139 (MQ=255)
                                                                                                          ccGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATc                                                <  1:364952/139‑1 (MQ=255)
                                                                                                           cGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGcccc                                     >  2:129703/1‑149 (MQ=255)
                                                                                                           cGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGcccc                                     >  3:534860/1‑149 (MQ=255)
                                                                                                           cGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGcccc                                     <  3:261847/149‑1 (MQ=255)
                                                                                                            ggACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcccc                                                         <  2:250477/128‑1 (MQ=255)
                                                                                                            ggACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcccc                                                         >  1:250477/1‑128 (MQ=255)
                                                                                                                ccGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGc                                >  3:13449/1‑149 (MQ=255)
                                                                                                                  ggAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCgg                              >  4:282843/1‑149 (MQ=255)
                                                                                                                     gagGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGaaa                           <  1:648015/149‑1 (MQ=255)
                                                                                                                      agGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAg                          >  3:714337/1‑149 (MQ=255)
                                                                                                                            gTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcga                    <  1:691205/149‑1 (MQ=255)
                                                                                                                               aaCCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcgacga                 >  2:431537/1‑149 (MQ=255)
                                                                                                                                   acaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCa             <  2:55733/149‑1 (MQ=255)
                                                                                                                                     aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga           <  1:3582/149‑1 (MQ=255)
                                                                                                                                         cTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAt       <  4:406087/149‑1 (MQ=255)
                                                                                                                                          tACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt      >  3:374249/1‑149 (MQ=255)
                                                                                                                                          tACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGACTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt      >  2:662097/1‑149 (MQ=255)
                                                                                                                                          tACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt      >  1:565749/1‑149 (MQ=255)
                                                                                                                                          tACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt      <  1:217445/149‑1 (MQ=255)
                                                                                                                                             accacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCaa   >  1:679561/1‑149 (MQ=255)
                                                                                                                                             accacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCaa   <  1:129703/149‑1 (MQ=255)
                                                                                                                                              ccacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAt  >  1:59263/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GAGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAAT  >  NC_000913/2873768‑2874058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑