Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,139,692 T→C 5.5% intergenic (+99/+24) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,139,6920TC 5.5% 235.6 / 3.5 73intergenic (+99/+24)yghU/hybGputative S‑transferase/hydrogenase 2 accessory protein
Reads supporting (aligned to +/- strand):  ref base T (32/37);  new base C (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

ATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCG  >  NC_000913/3139544‑3139839
                                                                                                                                                    |                                                                                                                                                   
aTGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCtt                                                                                                                                                     >  3:440613/1‑149 (MQ=255)
aTGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCtt                                                                                                                                                     <  3:468981/149‑1 (MQ=255)
aTGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCtt                                                                                                                                                     <  3:588082/149‑1 (MQ=255)
   aCGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATc                                                                                                                                                  <  1:2835/149‑1 (MQ=255)
      ccAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAgg                                                                                                                                               <  1:18409/149‑1 (MQ=255)
      ccAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAgg                                                                                                                                               >  3:442470/1‑149 (MQ=255)
       cAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGc                                                                                                                                              >  2:529979/1‑149 (MQ=255)
       cAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGc                                                                                                                                              >  1:383107/1‑149 (MQ=255)
       cAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGc                                                                                                                                              >  4:251482/1‑149 (MQ=255)
          tGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTa                                                                                                                                           >  2:621548/1‑149 (MQ=255)
          tGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTa                                                                                                                                           >  4:368530/1‑149 (MQ=255)
               tCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAGGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaaa                                                                                                                                      <  4:610984/149‑1 (MQ=255)
               tCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaaa                                                                                                                                      <  1:733888/149‑1 (MQ=255)
                 gagaCGAATACGGAAGATACGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGc                                                                                                                                    >  4:642984/1‑149 (MQ=255)
                 gagaCGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGACGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGc                                                                                                                                    <  3:609294/149‑1 (MQ=255)
                  agaCGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCt                                                                                                                                   >  2:771610/1‑149 (MQ=255)
                   gaCGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTa                                                                                                                                  <  1:251749/149‑1 (MQ=255)
                     cGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAat                                                                                                                                <  4:440613/149‑1 (MQ=255)
                     cGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAat                                                                                                                                <  4:686787/149‑1 (MQ=255)
                      gAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatc                                                                                                                               <  2:769699/149‑1 (MQ=255)
                        aTACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat                                                                                                                             >  2:531933/1‑149 (MQ=255)
                         tACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcatc                                                                                                                            <  1:761637/149‑1 (MQ=255)
                            ggAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCGGGCCTACGAAAGCTAATCATCAcg                                                                                                                         >  4:26074/1‑149 (MQ=255)
                             gAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc                                                                                                                        <  4:462004/149‑1 (MQ=255)
                               aGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACgcgc                                                                                                                      <  2:159054/149‑1 (MQ=255)
                                 aTAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg                                                                                                                    <  3:377218/149‑1 (MQ=255)
                                  tAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg                                                                                                                   >  1:151332/1‑149 (MQ=255)
                                   aaGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGt                                                                                                                  >  3:210240/1‑149 (MQ=255)
                                    aGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatc                                                                                                                               <  3:499902/135‑1 (MQ=255)
                                    aGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatc                                                                                                                               >  4:499902/1‑135 (MQ=255)
                                     gCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa                                                                                                                >  3:433788/1‑149 (MQ=255)
                                      cGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAAt                                                                                                               <  2:105002/149‑1 (MQ=255)
                                       gTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg                                                                                                              >  3:708679/1‑149 (MQ=255)
                                          aGGGGTAAGGGTTGGTGTTTGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCtt                                                                                                                                                     >  3:346123/1‑107 (MQ=255)
                                          aGGGGTAAGGGTTGGTGTTTGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCtt                                                                                                                                                     <  4:346123/107‑1 (MQ=255)
                                                   ggTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAtt                                                                                                  >  4:66935/1‑149 (MQ=255)
                                                   ggTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAtt                                                                                                  <  4:333013/149‑1 (MQ=255)
                                                    gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAttt                                                                                                 >  3:670891/1‑149 (MQ=255)
                                                    gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAttt                                                                                                 >  3:369617/1‑149 (MQ=255)
                                                     atGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTg                                                                                                <  3:66935/148‑1 (MQ=255)
                                                           ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAg                                                                                          >  4:728278/1‑149 (MQ=255)
                                                              gtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGc                                                                                       <  3:368530/149‑1 (MQ=255)
                                                                       aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAAtgt                                                                              <  4:433788/149‑1 (MQ=255)
                                                                           aTCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggct                                                                          >  2:470578/1‑149 (MQ=255)
                                                                           aTCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGCGCGTCTAATGtggct                                                                          >  4:296237/1‑149 (MQ=255)
                                                                             ccAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggcttt                                                                        <  1:603730/149‑1 (MQ=255)
                                                                             ccAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggcttt                                                                        <  1:483401/149‑1 (MQ=255)
                                                                              cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttg                                                                       <  1:599628/149‑1 (MQ=255)
                                                                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAt                                                                                                   <  2:624446/119‑1 (MQ=255)
                                                                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAt                                                                                                   >  1:624446/1‑119 (MQ=255)
                                                                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGtggctttggc                                                                     <  2:401453/149‑1 (MQ=255)
                                                                                   cGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTc                                                                  <  4:369617/149‑1 (MQ=255)
                                                                                      aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGt                                                                                     >  4:219962/1‑127 (MQ=255)
                                                                                      aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGt                                                                                     <  3:219962/127‑1 (MQ=255)
                                                                                         aGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtc                                                            <  1:771610/149‑1 (MQ=255)
                                                                                           gCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTtcgt                                                          >  1:109859/1‑149 (MQ=255)
                                                                                                 aCGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGAGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCgatga                                                    >  4:286949/1‑149 (MQ=255)
                                                                                                        aTCCGGCAATCGTGCACAATGCCTGCTGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCAt                                             <  3:496829/149‑1 (MQ=255)
                                                                                                        aTCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTATTCATAACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCAt                                             <  3:715761/149‑1 (MQ=255)
                                                                                                         tCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATg                                            <  3:322525/149‑1 (MQ=255)
                                                                                                         tCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATg                                            <  1:450245/149‑1 (MQ=255)
                                                                                                            ggCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCa                                         >  2:709996/1‑149 (MQ=255)
                                                                                                              cAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCaaa                                       >  3:596713/1‑149 (MQ=255)
                                                                                                                aTCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTACCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATc                                     >  4:228354/1‑149 (MQ=255)
                                                                                                                    tGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCTTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCACTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCgtc                                 <  4:670891/149‑3 (MQ=255)
                                                                                                                       acaATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGa                                  >  2:611958/1‑145 (MQ=255)
                                                                                                                       acaATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGa                                  <  1:611958/145‑1 (MQ=255)
                                                                                                                         aaTGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATg                                            >  2:305573/1‑133 (MQ=255)
                                                                                                                         aaTGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATg                                            <  1:305573/133‑1 (MQ=255)
                                                                                                                            gCCTGATAAGACGCGGCAGCGTCGCATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCAt                                                                <  4:441905/110‑1 (MQ=37)
                                                                                                                            gCCTGATAAGACGCGGCAGCGTCGCATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCAt                                                                >  3:441905/1‑110 (MQ=37)
                                                                                                                              cTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGc                       <  4:266273/149‑1 (MQ=255)
                                                                                                                              cTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGc                       >  3:724124/1‑149 (MQ=255)
                                                                                                                              cTGATAAGACGCGGCAGCGTCGCATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGc                       >  2:196564/1‑149 (MQ=255)
                                                                                                                                   gCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGCCGTGTACCAGCACCCa                  <  4:442470/149‑1 (MQ=255)
                                                                                                                                        gCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGc             <  4:708679/149‑1 (MQ=255)
                                                                                                                                            ccGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAg         >  3:352906/1‑149 (MQ=255)
                                                                                                                                              gcgTCGCATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTa       <  1:196564/149‑1 (MQ=255)
                                                                                                                                                   ttATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCg  >  1:68520/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGTGCGTCTAATGTGGCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCG  >  NC_000913/3139544‑3139839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: