Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,227,836 T→C 5.1% L12L (CTT→CTC ygjJ → putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,227,8360TC 5.1% 250.9 / 3.6 78L12L (CTT→CTCygjJputative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (40/34);  new base C (2/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-01

CAGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATAT  >  NC_000913/3227688‑3227979
                                                                                                                                                    |                                                                                                                                               
cAGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACtt                                                                                                                                                 <  1:31392/149‑1 (MQ=255)
 aGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTg                                                                                                                                                >  1:586668/1‑149 (MQ=255)
    cATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGctc                                                                                                                                             <  4:714383/149‑1 (MQ=255)
    cATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGctc                                                                                                                                             <  1:613845/149‑1 (MQ=255)
     aTTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGctct                                                                                                                                            >  1:259006/1‑149 (MQ=255)
           ccTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGtt                                                                                                                                      >  3:158471/1‑149 (MQ=255)
            ctctTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGttt                                                                                                                                     <  2:603177/149‑1 (MQ=255)
                  cTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTAc                                                                                                                               <  1:177338/149‑1 (MQ=255)
                  cTGGATGAGGACAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTAc                                                                                                                               >  3:491463/1‑149 (MQ=255)
                   tGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACg                                                                                                                              >  3:707436/1‑149 (MQ=255)
                    ggATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTCGCTCTGCCGTTTTGCTACGc                                                                                                                             >  1:213033/1‑149 (MQ=255)
                    ggATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTCGCTCTGCCGTTTTGCTACGc                                                                                                                             >  1:715826/1‑149 (MQ=255)
                     gATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGcc                                                                                                                            <  2:725448/149‑1 (MQ=255)
                       tGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCtt                                                                                                                          <  4:663358/149‑1 (MQ=255)
                          ggagAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTc                                                                                                                       >  3:675027/1‑149 (MQ=255)
                               gggAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCgg                                                                                                                  <  2:680517/149‑1 (MQ=255)
                                ggAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTTCGGc                                                                                                                 >  2:230349/1‑149 (MQ=255)
                                ggAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGAATTAACACAATGAAATTAATCACTGCACCATGCAAGGCATTACTTGCTCTGCCGTTTTGCTACTCCTTTTCTGCGGc                                                                                                                 >  4:334602/1‑149 (MQ=255)
                                 gAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCa                                                                                                                <  3:571107/149‑1 (MQ=255)
                                 gAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCa                                                                                                                >  3:239156/1‑149 (MQ=255)
                                  aaaaTAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAg                                                                                                               >  3:216926/1‑149 (MQ=255)
                                    aaTAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGga                                                                                                             <  1:230349/149‑1 (MQ=255)
                                     aTAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGgag                                                                                                            >  3:650341/1‑149 (MQ=255)
                                       aGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGagaa                                                                                                          <  1:389502/149‑1 (MQ=255)
                                            cTGCCTTTATATTTTTATAATTACAACGATAAAAGGTTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGc                                                                                                     <  4:513627/149‑1 (MQ=255)
                                            cTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGc                                                                                                     <  3:490794/149‑1 (MQ=255)
                                                cTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTACTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGt                                                                                                 >  2:132346/1‑149 (MQ=255)
                                                 tttATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTCATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGtt                                                                                                <  4:491536/149‑2 (MQ=255)
                                                 tttATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTc                                                                                                >  2:55789/1‑149 (MQ=255)
                                                  ttATATTTTTATAATTACAACGATAAAAGGCTTTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTTCACCATACAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTcc                                                                                               >  3:319377/1‑149 (MQ=255)
                                                  ttATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTCACACAATGAAATTACTCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGACAAGAAGCACGTct                                                                                               <  4:491463/149‑2 (MQ=255)
                                                  ttATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCAAGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGttt                                                                                                                                     <  4:502564/111‑1 (MQ=255)
                                                  ttATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCAAGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGttt                                                                                                                                     >  3:502564/1‑111 (MQ=255)
                                                   tataTTTTTATAATTACAACGATAAAACGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCg                                                                                              >  3:507811/1‑149 (MQ=255)
                                                        ttttATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGa                                                                                         >  4:99552/1‑149 (MQ=255)
                                                         tttATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCAGCAGaa                                                                                        <  2:591840/149‑1 (MQ=255)
                                                               aTTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgac                                                                                  >  3:217805/1‑149 (MQ=255)
                                                               aTTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgac                                                                                  <  4:507811/149‑1 (MQ=255)
                                                                ttACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgacg                                                                                 >  1:507611/1‑149 (MQ=255)
                                                                 tACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATgacga                                                                                <  4:325568/149‑1 (MQ=255)
                                                                    aaCGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGacac                                                                             <  2:586668/149‑1 (MQ=255)
                                                                      cGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACaa                                                                           >  2:702887/1‑149 (MQ=255)
                                                                          aaaaGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAGATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAac                                                                       >  1:694115/1‑149 (MQ=255)
                                                                          aaaaGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAac                                                                       >  1:292909/1‑149 (MQ=255)
                                                                            aaGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAacac                                                                     >  2:627509/1‑149 (MQ=255)
                                                                             aGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACAcc                                                                    <  1:702887/149‑1 (MQ=255)
                                                                              ggCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACAccc                                                                   <  2:201215/149‑1 (MQ=255)
                                                                                      tttttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTAc                                                           >  1:94591/1‑149 (MQ=255)
                                                                                       ttttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTAcc                                                          <  1:55789/149‑1 (MQ=255)
                                                                                         ttCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTc                                                        <  3:237963/149‑1 (MQ=255)
                                                                                            tttAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGAc                                                     >  2:138582/1‑149 (MQ=255)
                                                                                                  tCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAACAACACCCGCAATTACCTCGACAtcttc                                               >  3:53384/1‑149 (MQ=255)
                                                                                                  tCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACAtcttc                                               >  3:696728/1‑149 (MQ=255)
                                                                                                        aTTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTc                                         >  1:590935/1‑149 (MQ=255)
                                                                                                          tAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCAt                                       <  2:694115/149‑1 (MQ=255)
                                                                                                             cacaATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCTGTTTTGCTACGCCTTTTCTGCGGCAGGAGTAGAAGCACGTCAGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTc                                    <  2:368569/149‑1 (MQ=255)
                                                                                                               caATGAAATTAATCACTGCACCATGCAGAGCATTACTCGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGt                                  <  2:213033/149‑1 (MQ=255)
                                                                                                               caATGAAATTAATCACTGCACCATGCAGAGCATTACTCGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGt                                  <  2:715826/149‑1 (MQ=255)
                                                                                                                aaTGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGtt                                 >  1:537683/1‑149 (MQ=255)
                                                                                                                aaTGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGtt                                 >  2:115714/1‑149 (MQ=255)
                                                                                                                aaTGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGtt                                 >  3:107932/1‑149 (MQ=255)
                                                                                                                  tGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGtttt                               >  3:80549/1‑149 (MQ=255)
                                                                                                                     aaTTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTAc                            <  2:507611/149‑1 (MQ=255)
                                                                                                                     aaTTAATCACGGCACCATGCAGAGCATTACTTGCTCTGTCGTTTTGCTACGCCTTTTCTGGGGCAGGAGAAGGAGCGCGTCCGGCGGGACGTGACGACACAAAAACACCCGCAATTACCGCGACATCTTCTCCTTCATTTCGtttttgc                            >  4:10259/1‑147 (MQ=255)
                                                                                                                      aTTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACg                           <  1:123162/149‑1 (MQ=255)
                                                                                                                              cTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGagaag                                                                                                         <  1:384302/63‑1 (MQ=255)
                                                                                                                              cTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGagaag                                                                                                         >  2:384302/1‑63 (MQ=255)
                                                                                                                               tGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAg                  <  4:383878/149‑1 (MQ=255)
                                                                                                                               tGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAg                  >  3:489595/1‑149 (MQ=255)
                                                                                                                                 cACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAggg                <  4:158471/149‑1 (MQ=255)
                                                                                                                                    cATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGtt             >  4:570415/1‑149 (MQ=255)
                                                                                                                                     aTGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTATGGCGAATTAGGGGTtg            <  2:564122/149‑1 (MQ=255)
                                                                                                                                          gagCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGa       >  1:142692/1‑149 (MQ=255)
                                                                                                                                          gagCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGa       >  3:553813/1‑149 (MQ=255)
                                                                                                                                               ttACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTc                                    <  4:757727/115‑1 (MQ=255)
                                                                                                                                               ttACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTc                                    >  3:757727/1‑115 (MQ=255)
                                                                                                                                               ttACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGatatat  <  4:216926/149‑1 (MQ=255)
                                                                                                                                                  cTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAgg                 <  2:351303/131‑1 (MQ=255)
                                                                                                                                                  cTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAgg                 >  1:351303/1‑131 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CAGGCATTTTCCCTCTTCCTGGATGAGGAGAGGGAAAATAGTTTCTGCCTTTATATTTTTATAATTACAACGATAAAAGGCTGTACTTTTTCTTTAGCTCATGGATTAACACAATGAAATTAATCACTGCACCATGCAGAGCATTACTTGCTCTGCCGTTTTGCTACGCCTTTTCTGCGGCAGGAGAAGAAGCACGTCCGGCAGAACATGACGACACAAAAACACCCGCAATTACCTCGACATCTTCTCCTTCATTTCGTTTTTACGGCGAATTAGGGGTTGGTGGATATAT  >  NC_000913/3227688‑3227979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: