| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,334,703 | A→G | 10.3% | intergenic (+22/‑206) | ispB → / → sfsB | octaprenyl diphosphate synthase/malPQ operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,334,703 | 0 | A | G | 10.3% | 300.8 / 22.5 | 107 | intergenic (+22/‑206) | ispB/sfsB | octaprenyl diphosphate synthase/malPQ operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base A (45/50); new base G (4/7); total (49/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
GGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTG > NC_000913/3334557‑3334851 | ggACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgc < 2:290989/149‑1 (MQ=255) gcgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgc‑gcgc > 3:291584/1‑149 (MQ=255) gTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCgg > 3:41809/1‑149 (MQ=255) cAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGa < 3:720600/149‑1 (MQ=255) cAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGa < 2:15082/149‑1 (MQ=255) cGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGATga < 1:488419/149‑2 (MQ=255) gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCT‑GCGCGGGATgag < 2:703745/149‑3 (MQ=255) gCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTc > 1:425614/1‑149 (MQ=255) gCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCT‑GCGCGGGATgaggg < 3:208977/149‑5 (MQ=255) ccGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTcc < 4:353001/149‑1 (MQ=255) cGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGC‑GCTGCGCGGGATgagggga < 1:683917/149‑7 (MQ=255) gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAg < 3:704757/149‑1 (MQ=255) gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAg < 1:286718/149‑1 (MQ=255) gaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCCCAGC‑GCGCGGGATGGTTCCAGTAAggt > 3:595107/1‑147 (MQ=255) aagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTc < 2:351787/149‑1 (MQ=255) agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTcc < 2:265906/149‑1 (MQ=255) gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCa > 3:710983/1‑149 (MQ=255) gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCa < 1:430380/149‑1 (MQ=255) gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCa > 1:641062/1‑149 (MQ=255) aCAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaa > 3:711914/1‑149 (MQ=255) aCAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaa > 4:281702/1‑149 (MQ=255) aaaGCCATCGCAGCGTTGCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa > 3:251939/1‑137 (MQ=255) aaaGCCATCGCAGCGTTGCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa < 4:251939/137‑1 (MQ=255) aaaGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa < 2:425614/149‑1 (MQ=255) aaaGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATaaaaa < 2:178634/149‑1 (MQ=255) aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca < 2:624062/149‑1 (MQ=255) cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAGAACACTTAtt < 3:444710/149‑1 (MQ=255) cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAtt < 1:157292/149‑1 (MQ=255) cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAtt < 2:49206/149‑1 (MQ=255) cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAtt < 4:261164/149‑1 (MQ=255) cAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTAAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCa < 4:159540/149‑1 (MQ=255) cGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGct < 1:147049/149‑1 (MQ=255) gTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACCCTTATTCAGCTCTATAaca < 2:147189/149‑1 (MQ=255) cccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGa < 1:123570/149‑1 (MQ=255) cacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa < 2:358694/149‑1 (MQ=255) acCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGATGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCATC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACGTGAAAAATAg < 4:659200/149‑1 (MQ=255) ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg < 1:383609/149‑1 (MQ=255) ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg < 4:710983/149‑1 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa < 1:250720/102‑1 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTaa > 2:250720/1‑102 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg > 4:72817/1‑149 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg > 4:510552/1‑149 (MQ=255) cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg > 4:101512/1‑149 (MQ=255) ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa < 1:430338/145‑1 (MQ=255) ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa > 2:430338/1‑145 (MQ=255) ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa > 1:666195/1‑149 (MQ=255) tGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgt < 1:102079/149‑1 (MQ=255) gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCAt < 4:451216/149‑1 (MQ=255) gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCAt > 1:650509/1‑149 (MQ=255) aaGCACTCATCGGCGTCGCGCACATCGCGGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATACAGCTCTATAACACCTGAAAAATAGGGATGTGCATCtt > 2:763852/1‑149 (MQ=255) aaGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCtt > 3:578496/1‑149 (MQ=255) gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta < 4:747200/149‑1 (MQ=255) aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata > 1:294462/1‑149 (MQ=255) aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata > 1:113621/1‑149 (MQ=255) cTCATAGGCCTCGCGCACATTGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat > 4:482859/1‑149 (MQ=255) tCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACAcc < 4:548729/120‑1 (MQ=255) tCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACAcc > 3:548729/1‑120 (MQ=255) aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt > 2:389236/1‑149 (MQ=255) aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt > 1:669498/1‑149 (MQ=255) aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATAtt > 1:294842/1‑149 (MQ=255) cGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCt < 2:639461/149‑1 (MQ=255) gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt < 2:279167/149‑1 (MQ=255) gcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTca > 3:470748/1‑149 (MQ=255) cacaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcac < 4:416936/149‑1 (MQ=255) acaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca > 3:358031/1‑149 (MQ=255) tCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACActc < 2:750172/149‑1 (MQ=255) tCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACActc < 2:641062/149‑1 (MQ=255) tGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTa < 3:281702/149‑1 (MQ=255) gTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTAc < 3:686415/149‑1 (MQ=255) gATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttt > 1:116831/1‑149 (MQ=255) aTCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt > 3:478651/1‑149 (MQ=255) tCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttttt < 3:228538/149‑1 (MQ=255) cGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTa < 4:607513/149‑1 (MQ=255) ttAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTaga < 4:358031/149‑1 (MQ=255) ttAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTaga < 2:294462/149‑1 (MQ=255) tAATCCCCTCCCCTCATCCCGCGTAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTagag < 2:669498/149‑1 (MQ=255) aaTCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGc > 1:451803/1‑149 (MQ=255) aaTCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGc > 3:560031/1‑149 (MQ=255) cccctcccctCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGa < 2:626334/113‑1 (MQ=255) cccctcccctCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGa > 1:626334/1‑113 (MQ=255) ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa < 3:683531/149‑1 (MQ=255) ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa > 1:191444/1‑149 (MQ=255) ccctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa > 3:56882/1‑149 (MQ=255) cctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg < 2:650509/149‑1 (MQ=255) cctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg > 3:154238/1‑149 (MQ=255) ctcccctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAgg > 1:106404/1‑149 (MQ=255) cctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCAt > 4:754346/1‑149 (MQ=255) ctcATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATc > 2:110018/1‑149 (MQ=255) ccATCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTGACAGGAACTTTTTAGAGCAATAGGCCATCa > 3:453371/2‑149 (MQ=255) cATCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAGACCACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta < 3:630360/93‑1 (MQ=255) cATCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta > 4:630360/1‑93 (MQ=255) cATCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAg > 2:375742/1‑149 (MQ=255) cATCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTCAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAg < 4:453371/149‑1 (MQ=255) aTCCCGCGCGCT‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg > 4:165892/1‑149 (MQ=255) tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa < 4:40425/149‑1 (MQ=255) tCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa > 1:433242/1‑149 (MQ=255) ccGCNCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAAAATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGt > 3:459857/1‑149 (MQ=255) ccGCGCAGC‑GCGCGGGATGGTTCCGGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTCTATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGt > 4:141462/1‑149 (MQ=255) cgcAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGtata < 4:154238/149‑1 (MQ=255) aGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTg < 3:754346/149‑1 (MQ=255) | GGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGC‑GCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTG > NC_000913/3334557‑3334851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |