Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,825 G→C 8.0% P88A (CCC→GCC) ‡ ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,8250GC 8.0% 244.7 / 12.6 76P88A (CCC→GCC) ‡ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (37/32);  new base C (3/3);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-02

GCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGATTTC  >  NC_000913/3601679‑3601973
                                                                                                                                                  |                                                                                                                                                    
gCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCa                                                                                                                                                    >  4:748631/1‑149 (MQ=255)
  cTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGc                                                                                                                                                  <  4:227658/149‑1 (MQ=255)
      cTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCa                                                                                                                                              <  4:463442/149‑1 (MQ=255)
        cGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCaga                                                                                                                                            <  4:24908/149‑1 (MQ=255)
         ggCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTctgtt                                                                                                                                           <  2:445299/149‑5 (MQ=255)
             tGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAAt                                                                                                                                       <  1:625862/149‑1 (MQ=255)
              gCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATg                                                                                                                                      <  3:235139/149‑1 (MQ=255)
                  cTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGa                                                                                                                                  >  4:261273/1‑149 (MQ=255)
                     tgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTct                                                                                                                                              <  2:639940/134‑2 (MQ=255)
                     tgCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGCCCAGCGTct                                                                                                                                              >  1:639940/1‑133 (MQ=255)
                        caccacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAATAGTGATTTGCTGTGACGGATAATACTGCGCCGCAGCCTTGTTAACGTGTTTGTACCCCATACAACAGGCGTGGGGCAGCGTCAGAGAAATGGCGATAACCa                                                                                                                            <  2:319968/149‑1 (MQ=255)
                         accacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCAt                                                                                                                           <  1:494261/149‑1 (MQ=255)
                          ccacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATc                                                                                                                          >  3:595099/1‑149 (MQ=255)
                          ccacGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATc                                                                                                                          >  4:433781/1‑149 (MQ=255)
                                 cGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTa                                                                                                                   <  2:268448/149‑1 (MQ=255)
                                         cGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTGTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACAATCACCTTTAAAAAtgtg                                                                                                           >  2:273763/1‑149 (MQ=255)
                                          gTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTgg                                                                                                          <  4:705666/149‑1 (MQ=255)
                                           tCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGc                                                                                                         >  3:488856/1‑149 (MQ=255)
                                                 tCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaaa                                                                                                               <  3:621845/137‑1 (MQ=255)
                                                 tCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaaa                                                                                                               >  4:621845/1‑137 (MQ=255)
                                                   cAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGtt                                                                                                 >  1:675880/1‑149 (MQ=255)
                                                    aGCGTTTTTTGCAGATAAACAGTGATCTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTAAACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGttt                                                                                                >  3:635577/1‑149 (MQ=255)
                                                        ttttttGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCtt                                                                                            <  2:470977/149‑1 (MQ=255)
                                                           tttGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTc                                                                                         <  1:630826/149‑1 (MQ=255)
                                                           tttGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTc                                                                                         <  1:42665/149‑1 (MQ=255)
                                                                aGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATc                                                                                    >  2:318787/1‑149 (MQ=255)
                                                                aGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATc                                                                                    >  4:661780/1‑149 (MQ=255)
                                                                 gATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATcc                                                                                   >  4:21681/1‑149 (MQ=255)
                                                                  aTAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCt                                                                                  >  2:195631/1‑149 (MQ=255)
                                                                    aaaCAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGc                                                                                >  2:673914/1‑149 (MQ=255)
                                                                       cAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAAt                                                                             <  1:761287/149‑1 (MQ=255)
                                                                         gTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaa                                                                                                                >  4:148516/1‑112 (MQ=255)
                                                                         gTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTaaaa                                                                                                                <  3:148516/112‑1 (MQ=255)
                                                                           gATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGGAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGCTCCTGCAATgcgg                                                                         <  4:491364/149‑2 (MQ=255)
                                                                               tggagagACGGATAATACTTCGCCGCCGGCTGGTTAACGTTTTTCTACACCATATAGAAGACCAACGGCAGCGTACGAGCAATAGCGATAACCATCACCGTTAAAAGCGTGGAGAACGGTTTGCTTTTCAGCTCCAGCAATGCGCCGTg                                                                     <  2:56368/143‑1 (MQ=255)
                                                                                  ggTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGaa                                                                  <  4:537233/149‑1 (MQ=255)
                                                                                  ggTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGaa                                                                  >  1:172189/1‑149 (MQ=255)
                                                                                    tGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGACAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAgg                                                                <  3:58414/149‑1 (MQ=255)
                                                                                     gACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGc                                                               <  2:744490/149‑1 (MQ=255)
                                                                                         gATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAg                                                           >  3:439102/1‑149 (MQ=255)
                                                                                         gATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAg                                                           >  1:173924/1‑149 (MQ=255)
                                                                                          ataataCTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAgc                                                          >  1:669767/1‑149 (MQ=255)
                                                                                             ataCTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCa                                                       <  2:675880/149‑1 (MQ=255)
                                                                                               aCTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCAcc                                                     >  3:592413/1‑149 (MQ=255)
                                                                                                  gCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGt                                                  <  4:592413/149‑1 (MQ=255)
                                                                                                       gccgccTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                             <  1:180521/149‑1 (MQ=255)
                                                                                                        ccgccTGGTAAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTg                                            <  1:528272/149‑1 (MQ=255)
                                                                                                             tGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAAc                                       >  1:368325/1‑149 (MQ=255)
                                                                                                                ttAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATATCCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTc                                                <  4:244061/137‑1 (MQ=255)
                                                                                                                ttAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAg                                                                 <  4:308975/120‑1 (MQ=255)
                                                                                                                ttAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAg                                                                 >  3:308975/1‑120 (MQ=255)
                                                                                                                ttAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTc                                                >  3:244061/1‑137 (MQ=255)
                                                                                                                ttAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGtt                                    <  3:410597/149‑1 (MQ=255)
                                                                                                                     gTTTTTGTACACCATATAACAGACGCTGAGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGttt                               >  4:276772/1‑149 (MQ=255)
                                                                                                                            tACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTg                                                                                                           <  4:428135/66‑1 (MQ=255)
                                                                                                                            tACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTg                                                                                                           >  3:428135/1‑66 (MQ=255)
                                                                                                                              cacCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCa                                                                                        >  1:347700/1‑83 (MQ=255)
                                                                                                                              cacCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCa                                                                                        <  2:347700/83‑1 (MQ=255)
                                                                                                                              cacCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTa                      <  4:615800/149‑1 (MQ=255)
                                                                                                                                 cATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCg                   >  2:575467/1‑149 (MQ=255)
                                                                                                                                  atatAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCgg                  >  4:703529/1‑149 (MQ=255)
                                                                                                                                  atatAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCgg                  >  4:369050/1‑149 (MQ=255)
                                                                                                                                   tttctctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGt                 >  2:470088/8‑149 (MQ=255)
                                                                                                                                   tataACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGt                 >  4:649232/1‑149 (MQ=255)
                                                                                                                                      aaCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGtttt              <  3:264301/149‑1 (MQ=255)
                                                                                                                                       tctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                             <  2:497962/114‑1 (MQ=255)
                                                                                                                                       tctgACGCTGCCCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTAGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                             >  1:497962/4‑117 (MQ=255)
                                                                                                                                       aCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg             >  2:381433/1‑149 (MQ=255)
                                                                                                                                       aCAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTg             >  2:618911/1‑149 (MQ=255)
                                                                                                                                        cAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                             >  1:522314/1‑116 (MQ=255)
                                                                                                                                        cAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGtt                                             <  2:522314/116‑1 (MQ=255)
                                                                                                                                         aGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGc           >  3:56077/1‑149 (MQ=255)
                                                                                                                                         aGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGc           >  3:541362/1‑149 (MQ=255)
                                                                                                                                         aGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGc           >  1:142513/1‑149 (MQ=255)
                                                                                                                                          gACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCg          >  3:86352/1‑149 (MQ=255)
                                                                                                                                            cGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCCTTTCTGACCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAg        <  2:552171/149‑1 (MQ=255)
                                                                                                                                                gggCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGAtt    >  4:101269/1‑149 (MQ=255)
                                                                                                                                                gggCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGAtt    >  4:62269/1‑149 (MQ=255)
                                                                                                                                                  gCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGATTTc  >  4:228564/1‑149 (MQ=255)
                                                                                                                                                  gCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGATTTc  >  3:146894/1‑149 (MQ=255)
                                                                                                                                                  gCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGATTTc  <  3:351280/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCTTGCTCGGCCTGCAACTGTGCCACCACGCCCGCAGCAGCGTCATCGTCCAGCGTTTTTTGCAGATAAACAGTGATTTGCGGTGACGGATAATACTGCGTCGCCGCCTGGTTAACGTTTTTGTACACCATATAACAGACGCTGGGCAGCGTCAGAGAAATGGCGATAACCATCACCGTTAAAAACGTGGCGAACGGTTTGCTTTTCAGATCCTGCAATGCGCCGTGGAAGGCATAGCGCACCTGTTCGTTGAAAACGTTGGTTTTGCGATTTACCGGTTTTGGCGAGGATTTC  >  NC_000913/3601679‑3601973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: