Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,794,970 G→C 5.6% M14I (ATG→ATC waaF → ADP‑heptose:LPS heptosyltransferase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,794,9700GC 5.6% 1007.1 / 36.6 282M14I (ATG→ATCwaaFADP‑heptose:LPS heptosyltransferase II
Reads supporting (aligned to +/- strand):  ref base G (123/142);  new base C (7/9);  total (130/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 306 total aligned reads displayed.

ATCTGACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACGGAGCG  >  NC_000913/3794822‑3795117
                                                                                                                                                    |                                                                                                                                                   
  cTGACAAATCTGCGCGCTGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAACACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGCTGGCGACatgat                                                                                                                                                   <  4:640756/149‑1 (MQ=255)
  cTGACAAATCTGCGCGCGGCGGGTTACGACACACCGTTCAAAACCGTTGCTGAAGGTATAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgat                                                                                                                                                   <  2:124480/149‑1 (MQ=255)
  cTGACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgat                                                                                                                                                   >  2:482316/1‑149 (MQ=255)
   tGACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGAcatcatc                                                                                                                                                  <  2:560788/149‑2 (MQ=255)
   tGACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgatg                                                                                                                                                  >  2:364109/1‑149 (MQ=255)
    gACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgatga                                                                                                                                                 >  1:677449/1‑149 (MQ=255)
     aCAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgatgat                                                                                                                                                >  1:297818/1‑149 (MQ=255)
     aCAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgatgat                                                                                                                                                <  4:581322/149‑1 (MQ=255)
             gcgcgcGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCaaa                                                                                                                                        <  2:92419/149‑1 (MQ=255)
             gcgcgcGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCaaa                                                                                                                                        <  3:593939/149‑1 (MQ=255)
             gcgcgcGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCaaa                                                                                                                                        <  2:619829/149‑1 (MQ=255)
               gcgcgGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGt                                                                                                                                      <  1:409174/149‑1 (MQ=255)
                cgcgGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGtc                                                                                                                                     <  3:29430/149‑1 (MQ=255)
                   ggcggGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTctct                                                                                                                                  <  3:753254/149‑1 (MQ=255)
                      gggTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgatgat                                                                                                                                                <  3:709691/132‑1 (MQ=255)
                         ttACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCa                                                                                                                            >  3:537826/1‑149 (MQ=255)
                             gACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCt                                                                                                                        >  3:212398/1‑149 (MQ=255)
                             gACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCt                                                                                                                        >  4:193935/1‑149 (MQ=255)
                              aCAAACCTTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTc                                                                                                                       >  2:414487/1‑149 (MQ=255)
                               cAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTcc                                                                                                                      >  1:308943/1‑149 (MQ=255)
                                  aCCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATCATCATGTc                                                                                                                                             >  4:650667/1‑123 (MQ=255)
                                  aCCGATCAAAACCGTTGATGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAATAGCTCTGCTTGAAAATAAGGGTGATCGGCCCGTCTTGCGTTGGCGACATGATGATCTCGCAAAGTCTCTATCGCACGCTCCAgg                                                                                                                   >  2:647598/1‑149 (MQ=255)
                                     gTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGgcgc                                                                                                                >  4:446402/1‑149 (MQ=255)
                                           aaCCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATc                                                                                                          >  3:15153/1‑149 (MQ=255)
                                            aCCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATcc                                                                                                         >  3:375379/1‑149 (MQ=255)
                                               gTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCa                                                                                                      >  4:702650/1‑149 (MQ=255)
                                                ttGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAg                                                                                                     <  1:260251/149‑1 (MQ=255)
                                                    tGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGA                                                                                                 <  2:590471‑M1/149‑1 (MQ=255)
                                                    tGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGA                                                                                                 >  2:30181‑M1/1‑149 (MQ=255)
                                                     gAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGAT                                                                                                >  1:238324‑M1/1‑149 (MQ=255)
                                                        ggTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAAT                                                                                             <  3:113454‑M1/149‑1 (MQ=255)
                                                          tgtAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAAT                                                                                             >  1:299887‑M1/1‑147 (MQ=255)
                                                            tAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAt                                                                                         <  4:320778/149‑1 (MQ=255)
                                                            tAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAt                                                                                         <  4:558583/149‑1 (MQ=255)
                                                            tAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAt                                                                                         <  2:429629/149‑1 (MQ=255)
                                                            tAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAt                                                                                         >  2:278269/1‑149 (MQ=255)
                                                            tAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAt                                                                                         <  1:555479/149‑1 (MQ=255)
                                                             aaCGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATaatgg                                                                                        >  2:520884‑M1/1‑144 (MQ=255)
                                                              aCGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACatgatgat                                                                                                                                                <  3:732848/92‑1 (MQ=255)
                                                              aCGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAtgt                                                                                       <  2:316278/149‑1 (MQ=255)
                                                              aCGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAtgt                                                                                       <  1:190374/149‑1 (MQ=255)
                                                                ggAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGa                                                                                     <  3:319930/149‑1 (MQ=255)
                                                                  aaTACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATg                                                                                   <  4:283445/149‑1 (MQ=255)
                                                                      cATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCAc                                                                               <  2:388874/149‑1 (MQ=255)
                                                                              ggCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATgg                                                                       >  2:183117/1‑149 (MQ=255)
                                                                              ggCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGAT                                                                                                <  2:535655‑M1/124‑1 (MQ=255)
                                                                              ggCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGAT                                                                                                >  1:535655‑M1/1‑124 (MQ=255)
                                                                               gCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGt                                                                      <  1:151883/149‑1 (MQ=255)
                                                                                  gAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGcc                                                                   <  1:476232/149‑1 (MQ=255)
                                                                                  gAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGcc                                                                   <  3:524071/149‑1 (MQ=255)
                                                                                     tCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGAt                                                                                         >  4:749377/1‑124 (MQ=255)
                                                                                      ctcgACGCATAAGATCTCTGCATGAAAATACTGGTGATGGGCCCATCTTGGGTTAGCGACATGATGATGTCGCGAAGTCTATAGCGCACGCTCCAGGCACGCTGTCCCCAAACGATAATCGATGTGATGGCACTGGCGTGGTTCCGTcc                                                               >  2:308301/3‑149 (MQ=255)
                                                                                      cgcgACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTcc                                                               <  1:636416/149‑1 (MQ=255)
                                                                                      cgcgACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTcc                                                               <  4:214476/149‑1 (MQ=255)
                                                                                       gcgACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCa                                                              <  4:497143/149‑1 (MQ=255)
                                                                                        cgACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCat                                                             <  1:172348/149‑1 (MQ=255)
                                                                                         taCGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCAGCGGCATGGTGCCGTCTatt                                                            <  4:152479/148‑1 (MQ=255)
                                                                                         gACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCatt                                                            >  4:705562/1‑149 (MQ=255)
                                                                                            gCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATaatggacggcaccatgccggtgccatcacatcgatt                                                         <  3:585212‑M1/149‑37 (MQ=255)
                                                                                            gCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTatt                                                         >  2:226076‑M2/1‑146 (MQ=255)
                                                                                             cATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTatta                                                        <  2:746806‑M2/149‑5 (MQ=255)
                                                                                               tAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATaatggacggcaccatgccggtgccatcacatcgattatc                                                      <  2:152162‑M1/149‑40 (MQ=255)
                                                                                               tAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTattatc                                                      <  1:212365‑M2/149‑7 (MQ=255)
                                                                                                 agagCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCGAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCAACAATCGATGTGATTGCGCCGGCATGGTGCCGTCCATTattatcgc                                                    >  4:465753‑M2/1‑141 (MQ=255)
                                                                                                 agagCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTattatcgc                                                    >  4:606719‑M2/1‑141 (MQ=255)
                                                                                                 agagCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTattatcgc                                                    >  3:228251‑M2/1‑141 (MQ=255)
                                                                                                 agagCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTattatcgc                                                    <  4:587113‑M2/149‑9 (MQ=255)
                                                                                                  gagCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATcc                                                                                                         <  1:588537/95‑1 (MQ=255)
                                                                                                   agCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCgg                                                  >  2:570720/1‑149 (MQ=255)
                                                                                                    gCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGa                                                 <  3:446402/149‑1 (MQ=255)
                                                                                                        tGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGcc                                             <  4:371210/149‑1 (MQ=255)
                                                                                                        tGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGcc                                             <  1:753531/149‑1 (MQ=255)
                                                                                                           aTGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGa                                          >  4:114303/1‑149 (MQ=255)
                                                                                                            tGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGaa                                         <  3:659682/149‑1 (MQ=255)
                                                                                                                aaTACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGa                                                 >  4:709816/1‑137 (MQ=255)
                                                                                                                 aTACTTGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCGGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTaa                                    <  4:100783/149‑1 (MQ=255)
                                                                                                                     tGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGaa                                >  3:168351/1‑149 (MQ=255)
                                                                                                                      ggTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAg                               >  4:727453/1‑149 (MQ=255)
                                                                                                                         gATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTa                            >  4:678030/1‑149 (MQ=255)
                                                                                                                          aTCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTAt                           <  3:27114/149‑1 (MQ=255)
                                                                                                                           tCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTAtt                          >  3:189347/1‑149 (MQ=255)
                                                                                                                            cGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTg                                                                     <  3:426181/105‑1 (MQ=255)
                                                                                                                            cGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTg                                                                     >  4:426181/1‑105 (MQ=255)
                                                                                                                            cGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTc                         >  1:7384/1‑149 (MQ=255)
                                                                                                                             ggCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTcc                        >  4:142129/1‑149 (MQ=255)
                                                                                                                               cccGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTa                      >  1:373239/1‑149 (MQ=255)
                                                                                                                               cccGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTa                      <  3:571910/149‑1 (MQ=255)
                                                                                                                                ccGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTAt                     >  1:122358/1‑149 (MQ=255)
                                                                                                                                ccGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTAt                     <  2:660450/149‑1 (MQ=255)
                                                                                                                                ccGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTAGTCCTAt                     <  1:354648/149‑1 (MQ=255)
                                                                                                                                  gTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGc                   <  3:397364/149‑1 (MQ=255)
                                                                                                                                  gTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGc                   <  1:448795/149‑1 (MQ=255)
                                                                                                                                  gTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGc                   <  3:635043/149‑1 (MQ=255)
                                                                                                                                          tttgCGACATCATCATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTc                         >  4:252840/4‑135 (MQ=255)
                                                                                                                                          tttgCGACATCATCATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTACGCCTCTCGGt           >  2:550236/4‑149 (MQ=255)
                                                                                                                                          ttGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGt           <  1:115564/149‑1 (MQ=255)
                                                                                                                                             gCGACATCATCATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCAc        >  1:518345/1‑149 (MQ=255)
                                                                                                                                              cGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACg       <  2:19113/149‑1 (MQ=255)
                                                                                                                                               gACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACgg      >  1:431902/1‑149 (MQ=255)
                                                                                                                                               gACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATATGATGGCACCGGCATGGTGCCGTCCATTATTGTCGCGGATGACGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACgg      >  3:471979/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCTGACAAATCTGCGCGCGGCGGGTTACGACAAACCGTTCAAAACCGTTGCTGAAGGTGTAACGGAATACATGGCCTGGCTGAATCGCGACGCATAAGAGCTCTGCATGAAAATACTGGTGATCGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACGGAGCG  >  NC_000913/3794822‑3795117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: