Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,844,985 C→A 7.0% A341D (GCC→GAC)  adeD → cryptic adenine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,844,9850CA 7.0% 304.8 / 5.6 91A341D (GCC→GAC) adeDcryptic adenine deaminase
Reads supporting (aligned to +/- strand):  ref base C (37/47);  new base A (5/2);  total (42/49)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

ATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCAACACCATTGCC  >  NC_000913/3844841‑3845130
                                                                                                                                                |                                                                                                                                                 
aTGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGc                                                                                                                                                   <  1:245644/145‑1 (MQ=255)
aTGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGc                                                                                                                                                   >  2:245644/1‑145 (MQ=255)
 tGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGata                                                                                                                                              <  4:287380/149‑1 (MQ=255)
   aaGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATc                                                                                                                                            <  3:580448/149‑1 (MQ=255)
   aaGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATc                                                                                                                                            >  2:105202/1‑149 (MQ=255)
      gACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTc                                                                                                                                         <  4:397159/149‑1 (MQ=255)
          caTCGATGCCTTAATTCGCCGCCTGCTCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGACGATATCGGCCTgc                                                                                                                                     <  2:123764/149‑2 (MQ=255)
          caTCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGt                                                                                                                                     <  1:72905/149‑1 (MQ=255)
           aTCGCTGCCTTAATTCGCCGCCTGATCGAACAACACAATATGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCATTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGtt                                                                                                                                    <  4:473323/149‑1 (MQ=255)
           aTCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGtt                                                                                                                                    <  1:687772/149‑1 (MQ=255)
           aTCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGGCACCAGCTGGTCGATGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTTGCACCCGGCAAGCAGGCCGATATCGTCCTGtt                                                                                                                                    >  3:592089/1‑149 (MQ=255)
            tCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTg                                                                                                                                   >  4:661804/1‑149 (MQ=255)
               aTGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCg                                                                                                                                           <  2:386035/138‑1 (MQ=255)
               aTGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCg                                                                                                                                           >  1:386035/1‑138 (MQ=255)
                 gCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGa                                                                                                                              >  1:255924/1‑149 (MQ=255)
                   cTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATg                                                                                                                            >  1:532494/1‑149 (MQ=255)
                    ttAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATgc                                                                                                                           >  3:337275/1‑149 (MQ=255)
                      aaTTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATgcgc                                                                                                                         <  1:80621/149‑1 (MQ=255)
                      aaTTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATgcgc                                                                                                                         <  1:189693/149‑1 (MQ=255)
                        ttCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGtt                                                                                                                                    <  1:479998/136‑1 (MQ=255)
                        ttCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGtt                                                                                                                                    >  2:479998/1‑136 (MQ=255)
                           gccgccTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAg                                                                                                                    <  3:200817/149‑1 (MQ=255)
                                cTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAATCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCAc                                                                                                               >  4:618741/1‑149 (MQ=255)
                                 tGATCGAACAACACAATGCGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACg                                                                                                              >  3:350735/1‑149 (MQ=255)
                                  gATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACgg                                                                                                             >  1:222408/1‑149 (MQ=255)
                                    tCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTg                                                                                                           >  1:282864/1‑149 (MQ=255)
                                       aacaacACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTgcag                                                                                                        >  1:352139/1‑149 (MQ=255)
                                       aacaacACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTgcag                                                                                                        <  4:173272/149‑1 (MQ=255)
                                          aacaCAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGcagcag                                                                                                     <  1:723386/149‑1 (MQ=255)
                                           acacAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAgg                                                                                                    <  3:240708/149‑1 (MQ=255)
                                           acacAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAgg                                                                                                    >  4:67527/1‑149 (MQ=255)
                                             acaATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTa                                                                                                  >  1:279564/1‑149 (MQ=255)
                                              caATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTAc                                                                                                 <  2:529417/149‑1 (MQ=255)
                                                aTGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGtt                                                                                                                                    >  4:666655/1‑112 (MQ=255)
                                                aTGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGtt                                                                                                                                    <  3:666655/112‑1 (MQ=255)
                                                aTGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTg                                                                                               <  2:425658/149‑1 (MQ=255)
                                                 tgtgCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGACAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTgg                                                                                              <  2:90972/149‑1 (MQ=255)
                                                  gtgCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGt                                                                                             <  1:258357/149‑1 (MQ=255)
                                                      cGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGaaa                                                                                         <  3:661804/149‑1 (MQ=255)
                                                       gCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAg                                                                                        <  2:185071/149‑1 (MQ=255)
                                                         tGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGc                                                                                      <  4:337275/149‑1 (MQ=255)
                                                          gCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCg                                                                                     >  1:674591/1‑149 (MQ=255)
                                                           cATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGa                                                                                    <  4:350735/149‑1 (MQ=255)
                                                           cATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCACCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGa                                                                                    <  2:27918/149‑1 (MQ=255)
                                                               tgGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCg                                                                                >  1:391069/1‑149 (MQ=255)
                                                                    tatCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGa                                                                           >  1:136310/1‑149 (MQ=255)
                                                                       cgcgTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATgc                                                                        <  2:222408/149‑1 (MQ=255)
                                                                        gcgTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTgg                                                                                              >  2:485950/1‑126 (MQ=255)
                                                                        gcgTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTgg                                                                                              <  1:485950/126‑1 (MQ=255)
                                                                        gcgTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATgcg                                                                       >  4:146721/1‑149 (MQ=255)
                                                                            cGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCaaa                                                                   <  3:67527/149‑1 (MQ=255)
                                                                              ccAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAg                                                                                        <  2:406520/126‑1 (MQ=255)
                                                                              ccAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAg                                                                                        >  1:406520/1‑126 (MQ=255)
                                                                                     gTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTCCTGGCACCCGGCAAGCAGGCCGATATCCTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGATACTGGTGAAAGGCGa                                                                                    >  3:469602/1‑123 (MQ=255)
                                                                                     gTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGa                                                                                    <  4:469602/123‑1 (MQ=255)
                                                                                       cGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCg                                                        >  2:357703/1‑149 (MQ=255)
                                                                                          cGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGaa                                                     <  4:273759/149‑1 (MQ=255)
                                                                                            gcgcgcCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGTAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAaga                                                   <  3:65997/149‑1 (MQ=255)
                                                                                            gcgcgcCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAaga                                                   <  1:487310/149‑1 (MQ=255)
                                                                                            gcgcgcCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAaga                                                   <  3:547858/149‑1 (MQ=255)
                                                                                            gcgcgcCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAaga                                                   <  2:530184/149‑1 (MQ=255)
                                                                                              gcgcCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGt                                                 <  2:76116/149‑1 (MQ=255)
                                                                                                    cTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCga                                           <  1:113314/149‑1 (MQ=255)
                                                                                                       tGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGAc                                        <  3:385105/149‑1 (MQ=255)
                                                                                                          tCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTgg                                     >  3:596254/1‑149 (MQ=255)
                                                                                                          tCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTgg                                     >  3:308067/1‑149 (MQ=255)
                                                                                                          tCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTGGAGCGGTGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGGGCAAAGCTTACAGGCGGAAGAGTCGGCGGGACTgg                                     >  4:244349/1‑149 (MQ=255)
                                                                                                          tCTGAATCAACTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTgg                                     >  1:664973/1‑149 (MQ=255)
                                                                                                           cTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGc                                    <  2:255924/149‑1 (MQ=255)
                                                                                                           cTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGc                                    >  1:4328/1‑149 (MQ=255)
                                                                                                            tGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGca                                   <  3:146721/149‑1 (MQ=255)
                                                                                                                tCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAt                               >  1:150286/1‑149 (MQ=255)
                                                                                                                tCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAt                               <  2:352139/149‑1 (MQ=255)
                                                                                                                 cACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAtc                              <  1:337906/149‑1 (MQ=255)
                                                                                                                     tCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAtccgct                          >  3:58899/1‑149 (MQ=255)
                                                                                                                     tCGACTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAtccgct                          >  4:420044/1‑149 (MQ=255)
                                                                                                                       ggCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAtccgctcc                        <  2:4328/149‑1 (MQ=255)
                                                                                                                         cTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAtccgctccgc                      <  4:58899/149‑1 (MQ=255)
                                                                                                                              tGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCatat                 >  1:573789/1‑149 (MQ=255)
                                                                                                                               ggCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGGTGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATg                >  1:485984/1‑149 (MQ=255)
                                                                                                                                  aCCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCa             >  2:611390/1‑149 (MQ=255)
                                                                                                                                   cccGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCaa            <  2:391069/149‑1 (MQ=255)
                                                                                                                                   cccGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCaa            >  4:474895/1‑149 (MQ=255)
                                                                                                                                    ccGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAACCCTTACAGGCGGAAGAGTCGGCGCGACTGGCACAATCCGCTCCGCCATATGGCAac           <  2:279564/149‑1 (MQ=255)
                                                                                                                                        tcaacAGGACGATATCGGCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCAACACCa       >  4:137991/5‑149 (MQ=255)
                                                                                                                                        tcaacAGGACGATATCGGCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCAACACCa       >  3:204059/5‑149 (MQ=255)
                                                                                                                                          tacAGGACGATATCGGCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGTAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAtccgc                           >  1:725987/3‑127 (MQ=255)
                                                                                                                                          aacAGGACGATATCGGCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCTGCGAGACTGGCACAATCCGCTCCGCCATATGGCAACACCAtt     >  1:39785/3‑149 (MQ=255)
                                                                                                                                          aacAGGACGATATCGGCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGGCTGGCACAATCCGCTCCGCCATATGGCAACACCAtt     >  2:200186/3‑149 (MQ=255)
                                                                                                                                          aacAGGACGATATCGGCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAAtccgc                           <  2:725987/125‑1 (MQ=255)
                                                                                                                                          aGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCATAATCCGCTCCGCCATATGGCAACACCAtt     <  2:470246/149‑1 (MQ=255)
                                                                                                                                           gCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCAACACCATTg    <  2:282864/149‑1 (MQ=255)
                                                                                                                                             aGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCAACACCATTGcc  >  4:678393/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTACTGGCACCCGGCAAGCAGGCCGATATCGTCCTGTTGAGCGATGCGCGTAAGGTCACGGTGCAGCAGGTACTGGTGAAAGGCGAGCCGATTGATGCGCAAACCTTACAGGCGGAAGAGTCGGCGAGACTGGCACAATCCGCTCCGCCATATGGCAACACCATTGCC  >  NC_000913/3844841‑3845130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: